16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase

16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase (EC 2.1.1.182, S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase, KsgA, ksgA methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase.[1][2][3][4][5][6][7][8] This enzyme catalyses the following chemical reaction

4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA 4 Ribosomal RNA + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase
Identifiers
EC number2.1.1.182
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

KsgA introduces the dimethylation of adenine1518 and adenine1519 in 16S rRNA. Strains lacking the methylase are resistant to kasugamycin [1].

References
  1. Helser TL, Davies JE, Dahlberg JE (September 1971). "Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli". Nature. 233 (35): 12–4. doi:10.1038/newbio233012a0. PMID 4329247.
  2. Helser TL, Davies JE, Dahlberg JE (January 1972). "Mechanism of kasugamycin resistance in Escherichia coli". Nature. 235 (53): 6–9. doi:10.1038/newbio235006a0. PMID 4336392.
  3. van Buul CP, van Knippenberg PH (1985). "Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA". Gene. 38 (1–3): 65–72. doi:10.1016/0378-1119(85)90204-5. PMID 3905517.
  4. Formenoy LJ, Cunningham PR, Nurse K, Pleij CW, Ofengand J (1994). "Methylation of the conserved A1518-A1519 in Escherichia coli 16S ribosomal RNA by the ksgA methyltransferase is influenced by methylations around the similarly conserved U1512.G1523 base pair in the 3' terminal hairpin". Biochimie. 76 (12): 1123–8. doi:10.1016/0300-9084(94)90040-x. PMID 7538324.
  5. O'Farrell HC, Scarsdale JN, Rife JP (May 2004). "Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli". Journal of Molecular Biology. 339 (2): 337–53. doi:10.1016/j.jmb.2004.02.068. PMID 15136037.
  6. Poldermans B, Roza L, Van Knippenberg PH (September 1979). "Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions". The Journal of Biological Chemistry. 254 (18): 9094–100. PMID 383712.
  7. Demirci H, Belardinelli R, Seri E, Gregory ST, Gualerzi C, Dahlberg AE, Jogl G (May 2009). "Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine". Journal of Molecular Biology. 388 (2): 271–82. doi:10.1016/j.jmb.2009.02.066. PMC 2679894. PMID 19285505.
  8. Tu C, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X (March 2009). "Structural basis for binding of RNA and cofactor by a KsgA methyltransferase". Structure. 17 (3): 374–85. doi:10.1016/j.str.2009.01.010. PMC 2672589. PMID 19278652.
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