2'-hydroxybiphenyl-2-sulfinate desulfinase
In enzymology, a 2'-hydroxybiphenyl-2-sulfinate desulfinase (EC 3.13.1.3) is an enzyme that catalyzes the chemical reaction
- 2'-hydroxybiphenyl-2-sulfinate + H2O 2-hydroxybiphenyl + sulfite
| 2-(2-hydroxyphenyl)benzenesulfinate hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.13.1.3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are 2'-hydroxybiphenyl-2-sulfinate and H2O, whereas its two products are 2-hydroxybiphenyl and sulfite.
This enzyme belongs to the family of hydrolases, specifically those acting on carbon-sulfur bonds. The systematic name of this enzyme class is 2'-hydroxybiphenyl-2-sulfinate sulfohydrolase. Other names in common use include gene dszB-encoded hydrolase, 2-(2-hydroxyphenyl) benzenesulfinate:H2O hydrolase, DszB, HBPSi desulfinase, 2-(2-hydroxyphenyl) benzenesulfinate sulfohydrolase, HPBS desulfinase, 2-(2-hydroxyphenyl)benzenesulfinate hydrolase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase, and 2-(2-hydroxyphenyl)benzenesulfinate desulfinase.
Structural studies
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2DE2, 2DE3, and 2DE4.
References
- Oldfield C, Pogrebinsky O, Simmonds J, Olson ES, Kulpa CF (September 1997). "Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968)". Microbiology. 143 (9): 2961–73. doi:10.1099/00221287-143-9-2961. PMID 9308179.
- Nakayama N, Matsubara T, Ohshiro T, Moroto Y, Kawata Y, Koizumi K, Hirakawa Y, Suzuki M, Maruhashi K, Izumi Y, Kurane R (July 2002). "A novel enzyme, 2'-hydroxybiphenyl-2-sulfinate desulfinase (DszB), from a dibenzothiophene-desulfurizing bacterium Rhodococcus erythropolis KA2-5-1: gene overexpression and enzyme characterization". Biochimica et Biophysica Acta. 1598 (1–2): 122–30. doi:10.1016/s0167-4838(02)00365-5. PMID 12147352.
- Watkins LM, Rodriguez R, Schneider D, Broderick R, Cruz M, Chambers R, Ruckman E, Cody M, Mrachko GT (July 2003). "Purification and characterization of the aromatic desulfinase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase". Archives of Biochemistry and Biophysics. 415 (1): 14–23. doi:10.1016/S0003-9861(03)00230-3. PMID 12801508.