23S rRNA (guanine745-N1)-methyltransferase

23S rRNA (guanine745-N1)-methyltransferase (EC 2.1.1.187, Rlma(I), Rlma1, 23S rRNA m1G745 methyltransferase, YebH, RlmAI methyltransferase, ribosomal RNA(m1G)-methylase, rRNA(m1G)methylase, RrmA, 23S rRNA:m1G745 methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine745-N1)-methyltransferase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + guanine745 in 23S rRNA S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
23S rRNA (guanine745-N1)-methyltransferase
Identifiers
EC number2.1.1.187
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

The enzyme specifically methylates guanine745 at N1 in 23S rRNA.

References
  1. Liu M, Novotny GW, Douthwaite S (November 2004). "Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase". RNA. 10 (11): 1713–20. doi:10.1261/rna.7820104. PMC 1370659. PMID 15388872.
  2. Gustafsson C, Persson BC (January 1998). "Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant". Journal of Bacteriology. 180 (2): 359–65. PMC 106891. PMID 9440525.
  3. Das K, Acton T, Chiang Y, Shih L, Arnold E, Montelione GT (March 2004). "Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site". Proceedings of the National Academy of Sciences of the United States of America. 101 (12): 4041–6. doi:10.1073/pnas.0400189101. PMC 384692. PMID 14999102.
  4. Hansen LH, Kirpekar F, Douthwaite S (July 2001). "Recognition of nucleotide G745 in 23 S ribosomal RNA by the rrmA methyltransferase". Journal of Molecular Biology. 310 (5): 1001–10. doi:10.1006/jmbi.2001.4836. PMID 11501991.
  5. Liu M, Douthwaite S (April 2002). "Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria". Molecular Microbiology. 44 (1): 195–204. doi:10.1046/j.1365-2958.2002.02866.x. PMID 11967079.
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