ADA (buffer)

ADA is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.

ADA

Names
IUPAC name 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid[1]
Other names ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid
Identifiers
CAS Number	26239-55-4
3D model (JSmol)	Interactive image
EC Number	247-530-0
PubChem CID	117765
UNII	5C4R3O704E
InChI InChI=1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13) Key: QZTKDVCDBIDYMD-UHFFFAOYSA-N
SMILES C(C(=O)N)N(CC(=O)O)CC(=O)O
Properties
Chemical formula	C6H10N2O5
Molar mass	190.155 g/mol
Acidity (pKa)	6.6
Hazards
Main hazards	Irritant
GHS Signal word	Warning
GHS hazard statements	H302, H315, H319, H335
GHS precautionary statements	P261, P264, P270, P271, P280, P301+312, P302+352, P304+340, P305+351+338, P312, P321, P330, P332+313, P337+313, P362, P403+233, P405, P501
Flash point	Non-flammable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H⁺, Ca²⁺, and Mg²⁺, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na⁺/K⁺-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.[2]