C1QBP

C1QBP
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1P32, 3RPX
Identifiers
Aliases	C1QBP, GHABP1, SF2p32, gC1Q-R, gC1qR, p32, complement component 1, q subcomponent binding protein, complement C1q binding protein, COXPD33, SF2AP32
External IDs	OMIM: 601269 MGI: 1194505 HomoloGene: 31023 GeneCards: C1QBP
Gene location (Human)
Chr.	Chromosome 17 (human)[1]
End	5,448,830 bp[1]
Gene location (Mouse)
Chr.	Chromosome 11 (mouse)[2]
End	70,983,026 bp[2]
RNA expression pattern
	; ;
	More reference expression data
Gene ontology
Molecular function	• GO:0001106 transcription corepressor activity; • transcription factor binding; • GO:0001948 protein binding; • hyaluronic acid binding; • kininogen binding; • mitochondrial ribosome binding; • mRNA binding; • protein kinase C binding; • complement component C1q binding; • adrenergic receptor binding; • translation activator activity;
Cellular component	• cytosol; • extracellular region; • cell surface; • mitochondrial matrix; • nucleolus; • cytoplasm; • membrane; • mitochondrion; • cell nucleus; • extracellular; • cell membrane; • presynaptic active zone; • presynapse; • glutamatergic synapse; • GABA-ergic synapse;
Biological process	• blood coagulation, intrinsic pathway; • positive regulation of mitochondrial translation; • negative regulation of interferon-gamma production; • positive regulation of protein kinase B signaling; • negative regulation of MDA-5 signaling pathway; • regulation of transcription, DNA-templated; • adaptive immune response; • ribosome biogenesis; • negative regulation of defense response to virus; • immune system process; • mRNA processing; • negative regulation of transcription from RNA polymerase II promoter; • transcription, DNA-templated; • positive regulation of trophoblast cell migration; • positive regulation of substrate adhesion-dependent cell spreading; • mature ribosome assembly; • negative regulation of mRNA splicing, via spliceosome; • immune response; • RNA splicing; • positive regulation of neutrophil chemotaxis; • classical complement pathway; • positive regulation of apoptotic process; • phosphatidylinositol 3-kinase signaling; • innate immune system; • GO:0022415 viral process; • positive regulation of dendritic cell chemotaxis; • regulation of complement activation; • negative regulation of RIG-I signaling pathway; • positive regulation of cell adhesion; • negative regulation of interleukin-12 production; • apoptotic process;
	Sources:Amigo / QuickGO
Orthologs
	708
	12261
	ENSG00000108561
	ENSMUSG00000018446
	Q07021
	O35658; Q8R5L1
	NM_001212
	NM_007573
	NP_001203
	NP_031599
	Wikidata
View/Edit Human	View/Edit Mouse

Complement component 1 Q subcomponent-binding protein, mitochondrial is a protein that in humans is encoded by the C1QBP gene.[5][6][7]

The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein.[7]

Protein subunit

C1QBP is 282 amino acid in length and has three homologous subunit with its N-terminal 73 amino acid residues cleaved off to produce mature C1QBP. C1QBP appears as a monomer around 33 kDa on SDS-PAGE gel under both reducing and nonreducing condition but migrates as a trimer on size-exclusion chromatography (gel filtration).[8]

Protein structure

The crystal structure of C1QBP at 2.25 Å resolution shows a homotrimeric ring displaying symmetry. The individual subunits are held together by noncovalent interactions and forms a doughnut shaped quaternary structure with a central cavity of 20 Å in diameter. Each Subunit of C1QBP has seven β-strand (β1- β7) and three α-helices (α1- α3). C1QBP is negatively charged on its soluble face while the membrane face is predominantly positively charged.[9]

Interactions

C1QBP has been shown to interact with Protein kinase D1,[10] BAT2,[11] PRKCD,[10] PKC alpha[10] and Protein kinase Mζ.[10] Other interacting partners of C1QBP include protein domains from pathogens such as bacteria,[12] virus [13] and plasmodium falciparum.[14] Plasma proteins including fibrinogen, FXII and HK have been demonstrated to interact with C1QBP in a zinc dependent manner,.[15][16] Recently, a tumour homing peptide, LyP-1(CGNKRTRGC) has been shown to selectively bind to C1QBP in tumour expressing cells.[17]

References

GRCh38: Ensembl release 89: ENSG00000108561 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000018446 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Deb TB, Datta K (March 1996). "Molecular cloning of human fibroblast hyaluronic acid-binding protein confirms its identity with P-32, a protein co-purified with splicing factor SF2. Hyaluronic acid-binding protein as P-32 protein, co-purified with splicing factor SF2". J Biol Chem. 271 (4): 2206–12. doi:10.1074/jbc.271.4.2206. PMID 8567680.
Ghebrehiwet B, Lim BL, Peerschke EI, Willis AC, Reid KB (June 1994). "Isolation, cDNA cloning, and overexpression of a 33-kD cell surface glycoprotein that binds to the globular "heads" of C1q". J Exp Med. 179 (6): 1809–21. doi:10.1084/jem.179.6.1809. PMC 2191527. PMID 8195709.
"Entrez Gene: C1QBP complement component 1, q subcomponent binding protein".
Jiang, Jianzhong (1999). "rystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein". PNAS. 96 (7): 3572–3577. Bibcode:1999PNAS...96.3572J. doi:10.1073/pnas.96.7.3572. PMC 22335. PMID 10097078.
Jiang, Jianzhong (1999). "rystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein". PNAS. 96 (7): 3572–3577. Bibcode:1999PNAS...96.3572J. doi:10.1073/pnas.96.7.3572. PMC 22335. PMID 10097078.
Storz, P; Hausser A; Link G; Dedio J; Ghebrehiwet B; Pfizenmaier K; Johannes F J (August 2000). "Protein kinase C [micro] is regulated by the multifunctional chaperon protein p32". J. Biol. Chem. UNITED STATES. 275 (32): 24601–7. doi:10.1074/jbc.M002964200. ISSN 0021-9258. PMID 10831594.
Lehner, Ben; Semple Jennifer I; Brown Stephanie E; Counsell Damian; Campbell R Duncan; Sanderson Christopher M (January 2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. United States. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. ISSN 0888-7543. PMID 14667819.
Braun, Laurence; Ghebrehiwet, Berhane; Cossart, Pascale (2000). "gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes". The EMBO Journal. 19 (7): 1458–1466. doi:10.1093/emboj/19.7.1458. PMC 310215. PMID 10747014.
Kittlesen, David J.; Chianese-Bullock, Kimberly A.; Yao, Zhi Q; Braciale, Thomas J.; Hahn, Young S. (2000). "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin Invest. 106 (10): 1239–1249. doi:10.1172/jci10323. PMC 381434. PMID 11086025.
Magallón-Tejada, Ariel (2016). "Cytoadhesion to gC1qR through Plasmodium falciparum Erythrocyte Membrane Protein 1 in Severe Malaria". PLOS Pathog. 12 (11): e1006011. doi:10.1371/journal.ppat.1006011. PMC 5106025. PMID 27835682.
Pathak, Monika; Kaira, Bubacarr G.; Slater, Alexandre; Emsley, Jonas (2018). "Cell Receptor and Cofactor Interactions of the Contact Activation System and Factor XI". Frontiers in Medicine. 5: 66. doi:10.3389/fmed.2018.00066. PMC 5871670. PMID 29619369.
Peerschke, EI; Bayer, AS; Ghebrehiwet, B; Xiong, YQ (2006). "gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues in an animal model of infective endocarditis". Infect Immun. 74 (8): 4418–4423. doi:10.1128/iai.01794-05. PMC 1539591. PMID 16861627.
Paasonen, Lauri; Sharma, Shweta; Braun, Gary B.; Kotamraju, Venkata R.; Chung, Thomas D.Y.; She, Zhigang; Sugahara, Kazuki N; Yliperttula, Marjo; Wu, Bainan; Pellecchia, Maurizio; Ruoslahti, Erkki; Teesalu, Tambet (2017). "New p32/gC1qR Ligands for Targeted Tumor Drug Delivery". ChemBioChem. 17 (7): 570–575. doi:10.1002/cbic.201500564. PMC 5433940. PMID 26895508.

External links

Human C1QBP genome location and C1QBP gene details page in the UCSC Genome Browser.

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[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000108561 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000018446 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8567680-5] Deb TB, Datta K (March 1996). "Molecular cloning of human fibroblast hyaluronic acid-binding protein confirms its identity with P-32, a protein co-purified with splicing factor SF2. Hyaluronic acid-binding protein as P-32 protein, co-purified with splicing factor SF2". J Biol Chem. 271 (4): 2206–12. doi:10.1074/jbc.271.4.2206. PMID 8567680.

[pmid8195709-6] Ghebrehiwet B, Lim BL, Peerschke EI, Willis AC, Reid KB (June 1994). "Isolation, cDNA cloning, and overexpression of a 33-kD cell surface glycoprotein that binds to the globular "heads" of C1q". J Exp Med. 179 (6): 1809–21. doi:10.1084/jem.179.6.1809. PMC 2191527. PMID 8195709.

[entrez-7] "Entrez Gene: C1QBP complement component 1, q subcomponent binding protein".

[8] Jiang, Jianzhong (1999). "rystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein". PNAS. 96 (7): 3572–3577. Bibcode:1999PNAS...96.3572J. doi:10.1073/pnas.96.7.3572. PMC 22335. PMID 10097078.

[9] Jiang, Jianzhong (1999). "rystal structure of human p32, a doughnut-shaped acidic mitochondrial matrix protein". PNAS. 96 (7): 3572–3577. Bibcode:1999PNAS...96.3572J. doi:10.1073/pnas.96.7.3572. PMC 22335. PMID 10097078.

[pmid10831594-10] Storz, P; Hausser A; Link G; Dedio J; Ghebrehiwet B; Pfizenmaier K; Johannes F J (August 2000). "Protein kinase C [micro] is regulated by the multifunctional chaperon protein p32". J. Biol. Chem. UNITED STATES. 275 (32): 24601–7. doi:10.1074/jbc.M002964200. ISSN 0021-9258. PMID 10831594.

[pmid14667819-11] Lehner, Ben; Semple Jennifer I; Brown Stephanie E; Counsell Damian; Campbell R Duncan; Sanderson Christopher M (January 2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. United States. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. ISSN 0888-7543. PMID 14667819.

[12] Braun, Laurence; Ghebrehiwet, Berhane; Cossart, Pascale (2000). "gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes". The EMBO Journal. 19 (7): 1458–1466. doi:10.1093/emboj/19.7.1458. PMC 310215. PMID 10747014.

[13] Kittlesen, David J.; Chianese-Bullock, Kimberly A.; Yao, Zhi Q; Braciale, Thomas J.; Hahn, Young S. (2000). "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin Invest. 106 (10): 1239–1249. doi:10.1172/jci10323. PMC 381434. PMID 11086025.

[14] Magallón-Tejada, Ariel (2016). "Cytoadhesion to gC1qR through Plasmodium falciparum Erythrocyte Membrane Protein 1 in Severe Malaria". PLOS Pathog. 12 (11): e1006011. doi:10.1371/journal.ppat.1006011. PMC 5106025. PMID 27835682.

[15] Pathak, Monika; Kaira, Bubacarr G.; Slater, Alexandre; Emsley, Jonas (2018). "Cell Receptor and Cofactor Interactions of the Contact Activation System and Factor XI". Frontiers in Medicine. 5: 66. doi:10.3389/fmed.2018.00066. PMC 5871670. PMID 29619369.

[16] Peerschke, EI; Bayer, AS; Ghebrehiwet, B; Xiong, YQ (2006). "gC1qR/p33 blockade reduces Staphylococcus aureus colonization of target tissues in an animal model of infective endocarditis". Infect Immun. 74 (8): 4418–4423. doi:10.1128/iai.01794-05. PMC 1539591. PMID 16861627.

[17] Paasonen, Lauri; Sharma, Shweta; Braun, Gary B.; Kotamraju, Venkata R.; Chung, Thomas D.Y.; She, Zhigang; Sugahara, Kazuki N; Yliperttula, Marjo; Wu, Bainan; Pellecchia, Maurizio; Ruoslahti, Erkki; Teesalu, Tambet (2017). "New p32/gC1qR Ligands for Targeted Tumor Drug Delivery". ChemBioChem. 17 (7): 570–575. doi:10.1002/cbic.201500564. PMC 5433940. PMID 26895508.