Dark diversity

Common cowslip (Primula veris) is a frequent plant species in European grasslands. If the environmental conditions are suitable, but the cowslip is missing from studied grassland patch, it belongs to the dark diversity for given grassland.

Dark diversity is part of the species pool concept.[3] A species pool is defined as set of all species that are able to inhabit particular site and that are present in the surrounding region or landscape.[4] Dark diversity comprises species that belong to a particular species pool but that are not currently present at a site.[1] Dark diversity is related to "habitat-specific" or "filtered" species pool which only includes species that can both disperse to and potentially inhabit the study site.[3][4] For example, if fish diversity in a coral reef site has been sampled, dark diversity includes all fish species from the surrounding region that are currently absent but can potentially disperse to and colonize the study site. Because all sampling will also miss some species actually present at a site, we also have the related idea of 'phantom species' -- those species present at a site but not detected within the sampling units used to sample the community at that site.Beck, J.B.; Larget, B.; Waller, D.M. (2018). "Phantom species: Adjusting estimates of colonization and extinction for pseudo-turnover". Oikos. 127 (11): 1605–1618. doi:10.1111/oik.05114.</ref> The existence of these phantom species means that routine measures of colonization and extinction at a site will always overestimate true rates because of "pseudo-turnover."

Dark diversity name is borrowed from dark matter: matter which cannot be seen and directly measured, but its existence and properties are inferred from its gravitational effects on visible matter. Similarly, dark diversity cannot be seen directly when only the sample is observed, but it is present if broader scale is considered, and its existence and properties can be estimated when proper data is available. With dark matter we can better understand distribution and dynamics of galaxies, with dark diversity we can understand composition and dynamics of ecological communities.

Dark diversity is the counterpart of observed diversity (alpha diversity) present in a sample. Dark diversity is habitat-specific in respect that the study site must contain favorable ecological conditions for species belonging to dark diversity. The habitat concept can be narrower (e.g. microhabitat in an old-growth forest) or broader (e.g. terrestrial habitat). Thus, habitat specificity does not mean that all species in dark diversity can inhabit all localities within study sample, but there must be ecologically suitable parts.

Habitat-specificity is making the distinction between dark diversity and beta diversity. If beta diversity is the association between alpha and gamma diversity, dark diversity connects alpha diversity and habitat-specific (filtered) species pool. Habitat-specific species pool only these which can potentially inhabit focal study site.[1] Observed diversity can be studied at any scale, and sites with varying heterogeneity. This is also true for dark diversity. Consequently, as local observed diversity can be linked to very different sample sizes, dark diversity can be applied at any study scale (1x1 m sample in a vegetation, bird count transect in a landscape, 50x50 km UTM grid cell).

Region size determines likelihood of dispersal to study site and selecting appropriate scale depends on research question. For a more general study, a scale comparable to biogeographic region can be used (e.g. a small country, a state, or radius of few hundred km). If we want to know which species potentially can inhabit study site in the near future (for example 10 years), landscape scale is appropriate.

To separate ecologically suitable species, different methods can be used.[3] Environmental niche modelling can be applied for a large number of species. Expert opinion can be used.[5] Data on species' habitat preferences is available in books, e.g. bird nesting habitats. This can also be quantitative, for example plant species indicator values, according to Ellenberg. A recently developed method estimates dark diversity from species co-occurrence matrices.[6] An online tool is available for the co-occurrence method.[7]

Dark diversity allows meaningful comparisons of biodiversity. The community completeness index can be used:

${\displaystyle \log({observeddiversity}/{darkdiversity})}$.[8]

This express the local diversity at the relative scale, filtering out the effect of regional species pool. For example, if completeness of plant diversity was studied at the European scale, it did not exhibit the latitudinal pattern seen with observed richness and species pool values. Instead, high completeness was characteristic to regions with lower human impact, indicating that anthropogenic factors are among the most important local scale biodiversity determinants in Europe.[9]

Dark diversity studies can be combined with functional ecology to understand why species pool is poorly realized in a locality. For example, if functional traits were compared between grassland species in observed diversity and dark diversity, it becomes evident, that dark diversity species have in general poorer dispersal abilities.[10]

Dark diversity can be useful in prioritizing nature conservation,[11] to identify in different regions most complete sites. Dark diversity helps to estimate the relative loss of local diversity, but also restoration progress.[12][13][14] Dark diversity of alien species, weeds and pathogens can be useful to prepare for future invasions in time.

Recently, dark diversity concept was used in to explain mechanisms behind plant diversity-productivity relationship.[15]

• DarkDivNet - a global network to explore the dark diversity of plant communities
• Shiny Dark Diversity Calculator: an online tool for calculating dark diversity based on species' co-occurrences