High-field asymmetric-waveform ion-mobility spectrometry

High-field asymmetric-waveform ion-mobility spectrometry (FAIMS or RF-DC ion-mobility spectrometry) is an ion-mobility spectrometry technique in which ions at atmospheric pressure are separated by the application of a high-voltage asymmetric waveform at radio frequency (RF) combined with a static (DC) waveform applied between two electrodes.[1][2][3] Depending on the ratio of the high-field and low-field mobility of the ion, it will migrate toward one or the other electrode. Only ions with specific mobility will pass through the device.

Application

One application of FAIMS is as an additional separation step between the liquid chromatography separation and mass spectrometric analysis in Liquid chromatography–mass spectrometry (LC-MS) as used in proteomic studies. It allows for online fractionation of the analyte components to improve detection of peptides in complex samples. LC-MS uses the mass to charge ratio of peptide ions to analyse samples and the resulting spectra are compared to spectral reference libraries. FAIMS can be used to filter out "chemical noise", i.e. compounds whose spectra would interfere with the spectra of the desired compound, either by overlapping with the desired compound's spectra or adding additional peaks to the spectra. It can be used to filter out interfering ions and simultaneously select peptides with charge states that are optimal for analysis.[4]

A further advantage of this technique is that it can be used to select for peptide ions that are of low abundance in the sample. Such low abundance ions are often not analysed because of the limitations of the duty cycles of the mass spectrometers. By selectively removing the more abundant ions FAIMS can assist in distinguishing between ions with similar mass to charge ratios and can prevent a more abundant ion from masking the presence of a less abundant ion.[4]

Devices

Devices utilizing the principle of RF-DC ion-mobility spectrometry include handheld explosive trace detectors "MO-2M"[5] and "Pilot-M".[6]

See also

References
  1. Guevremont R (November 2004). "High-field asymmetric waveform ion mobility spectrometry: a new tool for mass spectrometry". J Chromatogr A. 1058 (1–2): 3–19. PMID 15595648.
  2. Kolakowski BM, Mester Z (September 2007). "Review of applications of high-field asymmetric waveform ion mobility spectrometry (FAIMS) and differential mobility spectrometry (DMS)". Analyst. 132 (9): 842–64. Bibcode:2007Ana...132..842K. doi:10.1039/b706039d. PMID 17710259.
  3. Лосев, В.В.; Кумпаненко, И.В.; Рощин, А.В. (2007). "Спектроскопия подвижности ионов — перспективный метод обнаружения химически опасных веществ в многокомпонентных воздушных средах" [Ion mobility spectroscopy is a promising method for the detection of chemically hazardous substances in multicomponent air environments]. Химическая физика [Chemical physics] (in Russian). 26 (10): 33–42.
  4. Swearingen, Kristian E; Moritz, Robert L (2012). "High-field asymmetric waveform ion mobility spectrometry for mass spectrometry-based proteomics". Expert Review of Proteomics. Informa UK Limited. 9 (5): 505–517. doi:10.1586/epr.12.50. ISSN 1478-9450. PMC 4777519.
  5. "Детектор паров взрывчатых веществ МО-2М". ООО "Сибел" (in Russian).
  6. Капустин, В.И.; Коржавый, А.П. (2016). "Дрейф-спектрометрия: традиционные методы и перспективы развития" [Drift spectrometry: traditional methods and development prospects]. Российский технологический журнал [Russian technological journal] (in Russian). 4 (2): 3–24. doi:10.32362/2500-316X-2016-4-2-3-24.
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