IAEDANS

IAEDANS
Names
	IUPAC name 5-[2-[(2-Iodo-1-oxoethyl)amino]ethylamino]-1-naphthalenesulfonic acid
	Other names 1,5-IAEDANS
Identifiers
CAS Number	36930-63-9 ;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:58984 ;
ChemSpider	82727 ;
ECHA InfoCard	100.048.418
PubChem CID	91621;
UNII	6V7G304M5M ;
CompTox Dashboard (EPA)	DTXSID10190421 ;
	InChI InChI=1S/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21) Key: ZMERMCRYYFRELX-UHFFFAOYSA-N ; InChI=1/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21) Key: ZMERMCRYYFRELX-UHFFFAOYAI;
	SMILES O=S(=O)(O)c1cccc2c1cccc2NCCNC(=O)CI;
Properties
Chemical formula	C14H15IN2O4S
Molar mass	434.25 g·mol−1
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

IAEDANS is an organic fluorophore (fluorescent molecule).[1] It stands for 5-({2-[(iodoacetyl)amino]ethyl}amino)naphthalene-1-sulfonic acid. It is widely used as a marker in fluorescence spectroscopy.

1,5-IAEDANS has a peak excitation wavelength of 336 nm and a peak emission wavelength of 490 nm. The extinction coefficient of the dye is 5700. It is soluble in dimethylformamide (DMF) or buffer above pH 6 and reacts primarily with thiols.

The absorption spectrum IAEDANS overlaps well with the emission spectrum of tryptophan, making it useful as an acceptor in FRET experiments. It can also be used as a resonance energy donor to fluorophores such as fluorescein, Alexa Fluor 488, Oregon Green, and BODIPY FL.

References

Takashi, R; Duke, J; Ue, K; Morales, M.F (1976). "Defining the "fast-reacting" thiols of myosin by reaction with 1,5 IAEDANS". Archives of Biochemistry and Biophysics. 175 (1): 279–83. doi:10.1016/0003-9861(76)90509-9. PMID 133635.

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[1] Takashi, R; Duke, J; Ue, K; Morales, M.F (1976). "Defining the "fast-reacting" thiols of myosin by reaction with 1,5 IAEDANS". Archives of Biochemistry and Biophysics. 175 (1): 279–83. doi:10.1016/0003-9861(76)90509-9. PMID 133635.