Lysine riboswitch

The Lysine riboswitch is a metabolite binding RNA element found within certain messenger RNAs that serve as a precision sensor for the amino acid lysine. Allosteric rearrangement of mRNA structure is mediated by ligand binding, and this results in modulation of gene expression.[1] Lysine riboswitch are most abundant in Firmicutes and Gammaproteobacteria where they are found upstream of a number of genes involved in lysine biosynthesis, transport and catabolism.[2][3][4] The lysine riboswitch has also been identified independently and called the L box.[5]

Lysine riboswitch
Predicted secondary structure and sequence conservation of Lysine riboswitch
Identifiers
SymbolLysine
RfamRF00168
Other data
RNA typeCis-reg; riboswitch
Domain(s)Bacteria
SOSO:0000035
PDB structuresPDBe

Structure

The structure of the lysine riboswitch has recently been determined.[6][7] The lysine amino acid is bound in the pocket formed by the 5-way junction. The structure is composed of a three helical bundle and a two helical bundle joined by the 5-way junction. Helices 1 and 2 are stacked in a colinear fashion as are helices 4 and 5.

  • A 3D representation of the lysine riboswitch with a bound lysine molecule shown in space filling spheres.[6]

References
  1. Mandal, M; Boese B; Barrick JE; Winkler WC; Breaker RR (2003). "Riboswitches Control Fundamental Biochemical Pathways in Bacillus subtilis and Other Bacteria". Cell. 113 (5): 577–586. doi:10.1016/S0092-8674(03)00391-X. PMID 12787499. S2CID 8012149.
  2. Sudarsan, N; Wickiser JK; Nakamura S; Ebert MS; Breaker RR (2003). "An mRNA structure in bacteria that controls gene expression by binding lysine". Genes Dev. 17 (21): 2688–2697. doi:10.1101/gad.1140003. PMC 280618. PMID 14597663.
  3. Rodionov, DA; Vitreschak AG; Mironov AA; Gelfand MS (2003). "Regulation of lysine biosynthesis and transport genes in bacteria: yet another RNA riboswitch?". Nucleic Acids Res. 31 (23): 6748–6757. doi:10.1093/nar/gkg900. PMC 290268. PMID 14627808.
  4. Mukherjee, S; Barash D; Sengupta S (2017). "Comparative genomics and phylogenomic analyses of lysine riboswitch distributions in bacteria". PLOS ONE. 12 (9): e0184314. doi:10.1371/journal.pone.0184314. PMC 5584792. PMID 28873470.
  5. Grundy, FJ; Lehman SC; Henkin TM (2003). "The L box regulon: Lysine sensing by leader RNAs of bacterial lysine biosynthesis genes". Proc Natl Acad Sci USA. 100 (21): 12057–12062. doi:10.1073/pnas.2133705100. PMC 218712. PMID 14523230.
  6. Serganov A, Huang L, Patel DJ (2008). "Structural insights into amino acid binding and gene control by a lysine riboswitch". Nature. 455 (7217): 1263–1267. doi:10.1038/nature07326. PMC 3726722. PMID 18784651.
  7. Garst AD, Héroux A, Rambo RP, Batey RT (August 2008). "Crystal structure of the lysine riboswitch regulatory mRNA element". The Journal of Biological Chemistry. 283 (33): 22347–22351. doi:10.1074/jbc.C800120200. PMC 2504901. PMID 18593706.
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