REV3L

Protein reversionless 3-like (REV3L) also known as DNA polymerase zeta catalytic subunit (POLZ) is an enzyme that in humans is encoded by the REV3L gene.[5][6][7]

REV3L
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesREV3L, POLZ, REV3, REV3 like, DNA directed polymerase zeta catalytic subunit
External IDsOMIM: 602776 MGI: 1337131 HomoloGene: 48147 GeneCards: REV3L
Gene location (Human)
Chr.Chromosome 6 (human)[1]
Band6q21Start111,299,028 bp[1]
End111,483,715 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

5980

19714

Ensembl

ENSG00000009413

ENSMUSG00000019841

UniProt

O60673

Q61493

RefSeq (mRNA)

NM_001286431
NM_001286432
NM_002912
NM_001372078

NM_011264

RefSeq (protein)

NP_001273360
NP_001273361
NP_002903
NP_001359007

NP_035394

Location (UCSC)Chr 6: 111.3 – 111.48 MbChr 10: 39.73 – 39.88 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

The Rev3 subunit interacts with Rev7 to form Pol ζ, a B family polymerase. Pol ζ lacks 3' to 5' exonuclease activity and is a moderate fidelity polymerase. It cannot add nucleotides across from DNA lesions, yet it can extend from primers with terminal mismatches. This makes Pol ζ very important in translesion synthesis (TLS), because it can act in concert with other TLS polymerases that can add across the lesion to complete the bypass of the lesion. Most polymerases have difficulty extending mismatches because they cannot bind properly to the mismatched DNA. So rather than the cell dying, it can survive albeit with a mutation that may or may not be deleterious, so it is believed that Pol ζ is a driving force of evolution.

Interactions

REV3L has been shown to interact with MAD2L2.[8][9]

References

  1. GRCh38: Ensembl release 89: ENSG00000009413 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000019841 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Gibbs PE, McGregor WG, Maher VM, Nisson P, Lawrence CW (Jul 1998). "A human homolog of the Saccharomyces cerevisiae REV3 gene, which encodes the catalytic subunit of DNA polymerase zeta". Proc Natl Acad Sci U S A. 95 (12): 6876–80. doi:10.1073/pnas.95.12.6876. PMC 22668. PMID 9618506.
  6. Morelli C, Mungall AJ, Negrini M, Barbanti-Brodano G, Croce CM (Mar 1999). "Alternative splicing, genomic structure, and fine chromosome localization of REV3L". Cytogenet Cell Genet. 83 (1–2): 18–20. doi:10.1159/000015157. PMID 9925914. S2CID 45239336.
  7. "Entrez Gene: REV3L REV3-like, catalytic subunit of DNA polymerase zeta (yeast)".
  8. Murakumo Y, Roth T, Ishii H, Rasio D, Numata S, Croce CM, Fishel R (February 2000). "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2". J. Biol. Chem. 275 (6): 4391–7. doi:10.1074/jbc.275.6.4391. PMID 10660610.
  9. Murakumo Y, Ogura Y, Ishii H, Numata S, Ichihara M, Croce CM, Fishel R, Takahashi M (September 2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7". J. Biol. Chem. 276 (38): 35644–51. doi:10.1074/jbc.M102051200. PMID 11485998.

Further reading


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