Sulforhodamine B
Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.[1]
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| IUPAC name
2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate | |
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| ECHA InfoCard | 100.020.482  |
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| Properties | |
| C27H30N2O7S2 | |
| Molar mass | 558.666 g/mol |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
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Spectroscopy
The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light.[1] It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.[2]
Applications
Sulforhodamine B is often used as a membrane-impermeable polar tracer[3] or used for cell density determination via determination of cellular proteins (cytotoxicity assay).[4]
References
- "Sulforhodamine B sodium salt (CAS 3520-42-1)". Santa Cruz Biotechnology.
- Coppeta, J.; Rogers, C. (1998). "Dual Emission Laser Induced Fluorescence for Direct Planar Scalar Behavior Measurements". Experiments in Fluids. 25: 1–15. doi:10.1007/s003480050202. S2CID 37649159.
- Viricel W; Mbarek A; Leblond J (2015). "Switchable Lipids: Conformational Change for Fast pH-Triggered Cytoplasmic Delivery" (PDF). Angewandte Chemie International Edition. 54 (43): 12743–12747. doi:10.1002/anie.201504661. PMID 26189870.
- Vichai V; Kirtikara K (2006). "Sulforhodamine B colorimetric assay for cytotoxicity screening". Nature Protocols. 1 (3): 1112–1116. doi:10.1038/nprot.2006.179. PMID 17406391. S2CID 1245354.
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