Aedes taeniorhynchus

Ae. taeniorhynchus swarms can be detected through sound. Noises with frequencies between 0.3 and 3.4 kHz at sound level 21 dB are detectable across 10–50 m in distance. An individual mosquito can be heard across 2–5 cm in distance when sound level rises to 22-25 dB.[7] Male and female mosquitoes can also be distinguished by their wingbeat frequencies, which are 700–800 Hz for males and 400–500 Hz for females.[7] As a result, flight sounds are used to determine flight activity and distinguish sex of groups.

Aedes mosquitoes have a characteristic microbiome that modulate the effects of diet.[15] Microbiome makeup is reported to differ between males and females in Aedes mosquitoes, such as Aedes albopictus[16] and Aedes aegypti.[17] Namely, in Aedes albopictus, males feed on nectar to acquire Actinobacteria while females contain Proteobacteria (such as Enterobacteriaceae) that mediate levels of redox stress caused by feeding on blood-meals.[16]

Aedes taeniorhynchus adult abdomen

The main physical distinctions between Ae. taeniorhynchus and other species come from the white banding that covers several body parts along Ae. taeniorhynchus. The species, like other Aedes mosquitoes, exhibits basal banding of the abdomen, but Ae. taeniorhynchus also uniquely exhibits white-tipped palps and a central white ring on the proboscis.[13]

This species looks similar to Aedes sollicitans, except for subtle differences in the larval and adult stages. In the larval stage, Ae. taeniorhynchus has a shorter breathing tube, its scale patches are rounded instead of pointed at the tips, and spines that line the edges of each scale patch are smaller near the scale patch base.[11] In the adult stage, Ae. taeniorhynchus is smaller and mostly black while Ae. sollicitans is golden brown.[11]

The species also bears similarity to Aedes jacobinae, which falls within the Taeniorhynchus subgenus due to its particular hypopygium structure, but it is considered a distinct species because it does not have leg markings.[18] Similarly, this species can also be distinguished from Aedes albopictus, commonly known as the Asian Tiger Mosquito, as Ae. taeniorhynchus, unlike Ae. albopictus, does not have markings on its back.[19]

Females lay eggs on dry ground, and egg hatching is triggered by the presence of water, such as rain or flooding.[31] Egg laying yield from females, an indicator of fecundity, differs based on diet: in populations of low autogeny, rare autogenous females each laid less than 30 eggs, while egg yield was significantly higher in populations with majority autogenous females.[25] Eggs laid in the right temperature and humidity conditions undergo embryogenesis, then stay dormant until hatching.[30]

4th larval instar

Pupa

Adult male

Aedes taeniorhynchus stages in life cycle

Upon hatching, the species progresses through 4 larval instars: the first 3 instars are affected primarily by temperature, with minor effects by salinity; the fourth instar is affected by all environmental factors.[30] In the fourth instar, increased food sped up development time while crowding and salinity stunted growth.[30]

Preferred temperature for all 4 instars is between 30 °C and 38 °C but average preferred temperature increases with age.[32] The first instar prefers an average temperature of 31.8 °C and the early fourth instar prefers a temperature of 34.6°.[32] The late fourth instar, however, has a lower preferred temperature than the early fourth instar, at 33.0°.[32] Starved larvae were found to have a wider preferred temperature range that is centered around lower temperatures.[32] Laboratory larval colonies cultured for years 27.0 °C were found to prefer consistently lower temperatures.[32]

Fourth-instar larvae were noted to drink sea water (100 nL/h) and secrete hyperosmotic fluid through the rectum.[33] This fluid is similar to seawater but with 18-fold higher potassium levels.[33] Because the secreted fluid does not allow for osmotic balance with the ingested fluid, studies suggest that the anal papillae aid in salt secretion.[33]

All environmental factors affect pupation regarding the insect's diurnal rhythm, which has a period of 21.5 hours.[30] Factors leading to an increased pupa period include erase of light-dark cycles with all dark or all light conditions, increased salinity, and crowding. These trends continued to adhere to a preference for temperatures close to 27 °C or 32 °C.[30] Pupa also exhibit differential aggregation formation due to these environmental factors. Cluster type aggregations form alongside temporary crowding and excess of food while ball type aggregations may manifest out of temporary crowding but lack of food.[34] At lower constant temperatures of 22 °C and 25 °C, cluster type aggregations may form but higher temperatures of 30° and 32 °C inhibit aggregation formation.[34] Aggregations produced pupa with slightly heavier dry body weights and promoted developmental synchronization in ecdysis and greater likelihood of migration at emergence.[34]

Males and females mosquitoes emerge from their egg sites in similar ways. They remain in their sources of water for 12–24 hours.[23] Adults then migrate away from the egg laying ground over the course of 1–4 days.[8] Different sexes exhibit differential migration, with most females traveling at least 20 mi (32 km), and most males traveling no farther than 2 mi (3.2 km).[8] Female migration follows a random pattern with no limitation on migration direction and migration occurring along a 5-day cycle.[8] Males initially travel with females until they hit a 1–2 mi (1.6–3.2 km) stopping point, where they replace migration with swarming.[8]

Flight patterns become established in the adult stage and are not affected by behavioral patterns from earlier stages of life.[6] Adults begin biting at day 4 and follow a 5-day cycle until death. Between the sexes, peak biting intensity occurs in females at ages 4, 9, and 14 days.[23] Adult female mosquitoes continue living and laying eggs for 3–4 weeks before dying.[23] Those that survive longer continue to bite but stop laying eggs.[23]

Adult female Aedes taeniorhynchus

Ae. taeniorhynchus eggs can mature both autogenously and anautogenously, with autogenous eggs feeding on sugar and anautogenous eggs requiring a blood meal.[35] These food sources promote maturation by producing hormones from the corpora allata (CA) and medial neurosecretory cell perikarya (MNCA), of which only MNCA hormone release is responsible for anautogenous maturation.[35] Larval dependence on a blood meal can be influenced to make mosquitoes less autogenous, by not allowing females to feed on sugar and imposing other dietary changes.[36]

Aedes taeniorhynchus is an ectoparasite of waved albatrosses

Adult mosquitoes feed on a combination diet of blood and sugar, with the optimal diet consisting of sugar for males and both blood and sugar for females.[5] Most Ae. taeniorhynchus rely on mammals and birds for blood meals, especially depending on bovine, rabbits, and armadillos.[37] Mosquitoes in the Galapagos Islands feed on mammals and reptiles, with equal preference but feed little on birds.[38] Since this differs from the typical feeding of Ae. taeniorhynchus on birds, studies suggest the species is an opportunistic feeder, in which it feeds more on the most readily available, easily accessible organisms.[38] Ae. taeniorhynchus acts as an ectoparasite to Diomedea irrorata, known as waved albatrosses.[39] Mosquitoes bite the waved albatrosses, directly leading to or transmitting diseases that cause nestling mortality, colony migration, or egg desertion in albatrosses.[39]

Experimental studies show that both sexes can survive on a sugar-only diet for 2–3 months, but females require blood meals for egg production.[40] In females, supplementation of a blood meal in autogenous mosquitoes increased both egg production and lifespan.[40] Additional observational studies of Ae. taeniorhynchus in nature showed that habitat impacts the effect of the meal source: females inhabiting mangrove swamps could produce eggs even without blood meals, but those from a grassy salt marsh environment could not.[41] Females from both habitats, however, were still able to produce eggs when given blood meals.[41]

Studies observing unrestricted sugar intake of females correlated sucrose intake level with maximum accumulation of stored energy reserves.[42] In contrast, sucrose intake level does not correlate with decreased activity or changes in senescence.[42]

Carbohydrate feedings of female mosquitoes in a laboratory setting indicated that carbohydrates glucose, fructose, mannose, galactose, sucrose, trehalose, melibiose, maltose, raffinose, melizitose, dextrin, mannitol, and sorbitol are most effective to aid survival; arabinose, rhamnose, fucose, sorbose, lactose, cellobiose, inulin, a-methyl mannoside, dulcitol, and inositol are not used by the species; xylose, glycogen, a-methyl glucoside, and glycerol are used but at a slow metabolic rate; and sorbose could not be metabolized.[43] Feeding with glucose allowed for maximum flight speed while other carbohydrates, such as all pentoses, sorbose, lactose, cellobiose, glycogen, inulin, a-methyl mannoside, dulcitol, and inositol were insufficient to allow flight, indicated by a delay in flight after feeding.[29]

If emergence occurs at a location with flowers, both sexes feed on nectar prior to migration.[44] Analysis of fructose and glycogen content indicate that mosquitoes often feed on nectar soon after dark and feed sparingly on nectar during the day.[45]

Males become sexually mature about 2 days after emergence, and females become sexually mature at an age of 12 days, with plans to mate only once.[21]

Observational studies of mating interactions both in a laboratory setting and field setting noted copulation between mosquitoes occurring after sunset. Results noted that copulation depends on age of females, with insemination occurring with females of ages 30–40 hours.[46] In both settings studied, females are capable of mating without inducing insemination, as only 1% of females contained sperm after 2 notes of potential mating.[46] Mating not only provides an opportunity for insemination but also contributes to vitellogenin synthesis in females, as experimental injections of male accessory gland fluid (MAGF) has been shown to cause release of corpus cardiacum (CC) stimulating factor in the ovaries, which spurs research of egg development neurosecretory hormone (EDNH).[47]

Other laboratory studies of the species noted an age dependence in both females and males for successful copulation and insemination.[48] Copulation is initiated by males and only occurs when the male first disengages its legs, interlocks the male and female genitalia in an end-to-end position, and then hangs from the female for a short duration of time.[48] Insemination can only result from copulation.[48] If copulation is successful, the mosquitoes pair in flight, then land and remain together for a few seconds.[48] To end copulation, the male flies away or the female flies while carrying the male until it falls.[48]

Most young females rejected copulation attempts (unreceptive), and many of those that copulated rejected insemination attempts (refractory), with acceptance of copulation and insemination (receptive) both increasing with female age when exposed to an older male cohort.[48] Unreceptive females avoided males by flying away with sudden increases in speed or sharp turns.[48]

During mating, males can transfer substances produced from their accessory glands that affect female physiology and behavior.[49] These accessory gland substances can limit or improve female reproductive activities.[49] Limitations include temporarily prevention of future female mating, oviposition stimulation, and reduced host-seeking while improvements involve changes to female circadian rhythm and metabolic priorities that cause higher chance of reproduction.[49]

Females are known to practice oviposition, with preference for high moisture soils, with water saturation greater than 70%.[50] Female clutch sizes are 100-200 eggs, with at least one clutch laid per female.[21]

In experimental studies with ovariectomized female mosquitoes, females were unable to synthesize vitellogenin, a yolk-protein precursor, unless given a donor ovary from a sugar-fed or blood-fed mosquito.[51] Vitellogenin synthesis still occurred when the donor ovary came from Ae. aegypti, and ovary derivation from a blood-fed mosquito caused corpus cardiacum stimulating factor production, indicating that the hormonal processes for oviposition are not species specific.[51]

In a study of eggs laid in Rhizophora mangle L. (red mangrove) and Avicennia germinans L. (black mangrove) forest basins, egg occurrence was correlated with elevation and detritus level.[52] Oviposition was directed from black mangrove basins to red mangrove basins, possibly due to reduced detritus and reduced organic content in the soil caused by black mangrove grazing by Melampus coffeus L., a snail.[52] Because eggshells and eggs share the same habitat, it is suggested that oviposition may be delineated using eggshells.[52] Eggshell sampling analysis from 34 mangrove forest sites indicated that all mangrove basin forests can yield successful Ae. taeniorhynchus production, regardless of forest geomorphology, soil, and vegetation but recently flooded sites are most optimal.[53]

Additionally, sulfates and other salts were deemed favorable to ovipositing females in a laboratory setting but sulfate concentrations in the field may be too low for this effect to be significant.[54] Substrate texture was also determined to be a factor contributing to oviposition, with studies of egg laying on sand particle size indicating a preference for sand particles sized from 0.33 to 0.62 mm.[55]

Adult female mosquitoes ready to lay eggs differ from other adult females in many important behaviors. They perform a special flight at ages 7, 12, and 17, following a 5-day cycle.[23] Changes in diet have effects on flight in males sand females: males fed sugar alone exhibited changes in flight patterns that resembled cyclic swarming, females fed sugar alone exhibited consistent flight patterns consisting of a 4-week cycle of flight 40 minutes during dark and 20 minutes during light, females fed sugar and blood experienced reduced flight after 2 weeks, and females fed blood alone flew no more than 10 days.[40] Starved females later fed blood stayed sedentary for 8 hours before returning to flight.[40] Flights are occur with the purpose of acquiring nectar, with flight distance depending on wind speed, direction, landscape, and nectar availability.[21] Females typically fly 2–5 miles in search of nectar, but flights ranging 30 miles have been recorded as a result of other flight factors.[21] Adults searching for a blood meal may also fly up to 25 miles.[19]

Flight patterns are these mosquitoes are closely related to light sensitivity, as flight patterns increase with strength of moonlight: females increase flight activity from 95% at quarter moon to 546% at full moon.[56] Male and female adult mosquitoes are repelled by light,[31] allowing mosquitoes to be caught with light traps.[8][57] However, females ready to lay eggs to not exhibit this behavior.[23] In an experimental setting, mosquitoes raised under conditions of 12 hr light : 12 hr dark were found to exhibit flight activity at both light-off and light-on periods in a bimodal alternans pattern. Mosquitoes adjusted to new light conditions within 24–36 hours, in which a delayed light-off resets the pattern but an early light-off does not.[6]

Adult males begin forming top-swarms beginning at an age of 4 days and lasting until 2–3 weeks of age.[23] These swarms form every evening and morning at a fixed location and time[23] and last for a maximum of 30 minutes.[21] In field observations of Ae. taeniorhynchus in Florida, morning and evening swarms were typically halfway finished by the time point of 4 minutes before and after twilight, respectively.[23] The initial stimulus for swarming behavior is unknown, but time spent swarming depends on sensitivity of individual males to the swarming driving force and swarm size, with small swarms lasting for 12 minutes and large swarms lasting for 27 minutes.[23] These swarms are characterized as transient passage swarms, where males participate in the swarm for 1.5 minutes at a time rather than the full-time.[23] Despite the act of males forming top-swarms, mating has not been observed to coincide with swarming.[23]