Chestnut blight

The pathogenic fungus Cryphonectria parasitica (formerly Endothia parasitica) is a member of the Ascomycota (sac fungi). It is native to East Asia and South East Asia and was introduced into Europe and North America in the 1900s.[1] The fungus spread rapidly and caused significant tree loss in both regions.

Chestnut blight fungus
Cankers caused by the fungal infection cause the bark to split.
Scientific classification
Kingdom: Fungi
Division: Ascomycota
Class: Sordariomycetes
Order: Diaporthales
Family: Cryphonectriaceae
Genus: Cryphonectria
Species:
C. parasitica
Binomial name
Cryphonectria parasitica
(Murrill) M.E.Barr (1978)
Synonyms
  • Diaporthe parasitica Murrill (1906)

Overview

Cryphonectria parasitica is a parasitic fungus of chestnut trees. This disease came to be known as chestnut blight. Naturally found in South East Asia, accidental introductions led to invasive populations of C. parasitica in North America and Europe. The fungal disease has had a devastating economic and social impact on communities in the eastern United States. In the first half of the 20th century it killed an estimated four billion trees. Less severe impacts have occurred in Europe due to widespread CHV1 hypovirulence. CHV1 is one of at least two viral pathogens that weaken the fungus through hypovirulence and helps trees survive.[2][3]

The American chestnut and American chinquapin are highly susceptible to chestnut blight. The European chestnut is also susceptible but due to widespread CHV1 hypoviruluence, blight-induced tree death is less common.[4] The fungus can infect other tree species such as oaks, red maples, staghorn sumacs, and shagbark hickories.[5] Once infected, these trees will also exhibit orange bark with cankers. However, they will not exhibit shoot die back and death of the main tree. Instead the pathogen can persist in trees, but the fungus will spore and so may infect other trees. The fungus is spread by wind-borne ascospores and, over a shorter distance, conidia distributed by rain-splash action.[6] Infection is local in range, so some isolated American chestnuts survive where there is no other tree within 10 km (6.2 mi). The root collar and root system of the chestnut tree have some resistance to blight infection due to soil organisms adversely reacting to the fungus; consequently, a large number of small American chestnut trees still exist as shoots growing from existing root bases. However, these regrown shoots seldom reach the sexually reproductive stage before being killed by the fungus.[7]

History

North American infection

The chestnut blight was accidentally introduced to North America around 1904 when Cryphonectria parasitica was introduced into the United States from East Asia from the introduction of the cultivation of Japanese chestnut trees into the United States for commercial purposes.[8][9] It was first found in the chestnut trees on the grounds of the New York Zoological Garden (the "Bronx Zoo") by Herman W. Merkel, a forester at the zoo. In 1905, American mycologist William Murrill isolated and described the fungus responsible (which he named Diaporthe parasitica), and demonstrated by inoculation into healthy plants that the fungus caused the disease.[10] By 1940, most mature American chestnut trees had been wiped out by the disease.[11]

Infection of American chestnut trees with C. parasitica simultaneously appeared in numerous places on the East Coast, most likely from Castanea crenata, or Japanese chestnut, which had become popular imports.[12] Japanese and Chinese chestnut trees[13] have resistance to infection by C. parasitica: the infection usually does not kill these Asian chestnut species. Within 40 years the nearly four-billion-strong American chestnut population in North America was devastated;[14] only a few clumps of trees remained in Michigan, Wisconsin and the Pacific Northwest. Because of the disease, American chestnut wood almost disappeared from the market for decades, although it can still be obtained as reclaimed lumber.[15]

It is estimated that in some places, such as the Appalachian Mountains, one in every four hardwoods was an American chestnut. Mature trees often grew straight and branch-free for 50 feet and could grow up to 100 feet tall with a trunk diameter of 14 feet at a few feet above ground level. The reddish-brown wood was lightweight, soft, easy to split, very resistant to decay; and it did not warp or shrink. For three centuries many barns and homes near the Appalachian Mountains were made from American chestnut.[16] Because of its resistance to decay, industries throughout the region used wood from the American chestnut for posts, poles, piling, railroad ties, and split-rail fences.[17] Its straight-grained wood was ideal for building furniture and caskets. The fruit that fell to the ground was an important cash crop and food source. The bark and wood were rich in tannic acid, which provided tannins for use in the tanning of leather.[18] Many native animals fed on chestnuts, and chestnuts were used for livestock feed, which kept the cost of raising livestock low.[19]

Efforts started in the 1930s and are still ongoing, in Massachusetts[20] and many other places[17] in the United States, to repopulate the country with chestnut trees.[21] Surviving American chestnut trees are being bred for resistance to the blight, notably by The American Chestnut Foundation, which aims to reintroduce a blight-resistant American chestnut to its original forest range within the early decades of the 21st century.[22] Japanese chestnut and Chinese chestnut, as well as Seguin's chestnut and Henry's chestnut, have been used in these breeding programs in the US to create disease-resistant hybrids with the American chestnut.[23] Chinese chestnut trees have been found to have the highest resistance to chestnut blight;[13] however, individuals within the Chinese chestnut species may vary in blight resistance. Some individuals are still quite susceptible while others are essentially immune.[24]

Hypovirulence is not widespread in the US and attempts to commercially introduce CHV1 have not been widely successful. Though CHV1 persists in the applied tree, it does not spread naturally as it does in Europe, preventing it from being an effective form of biocontrol.

European infection

Chestnut blight was first identified around Genoa in 1938. This quickly spread and was identified in France in 1946, Switzerland in 1951 and in Greece in 1963. It has most recently been found in the UK. Due to genetic differences between the fungal populations, it is likely that a second introduction of chestnut blight occurred in Georgia and Azerbaijan in 1938.[25][26] The fungal infections initially caused widespread tree death in Europe. However, in the early 1950s trees were identified in Italy that survived fungal infection. On these trees the fungus caused more superficial cankers, that appeared to be healing. The reduced infection was due to the presence of CHV1, an RNA virus that infects C. parasitica. CHV1 spread naturally throughout Europe but is also spread artificially as a biocontrol measure (particularly in France). CHV1 is currently not present in the UK, Northern France or Eastern Georgia but introduction for biocontrol is being considered.

Symptoms
A chestnut tree that has been felled, with blight on its inner bark and trunk

The fungus enters through wounds on susceptible trees and grows in and beneath the bark, eventually killing the cambium all the way round the twig, branch or trunk.[27] The first symptom of C. parasitica infection is a small orange-brown area on the tree bark. A sunken canker then forms as the mycelial fan spreads under the bark. As the hyphae spread, they produce several toxic compounds, the most notable of which is oxalic acid. This acid lowers the pH of the infected tissue from around the normal 5.5 to approximately 2.8, which is toxic to plant cells. The canker eventually girdles the tree, killing everything above it. Distinctive yellow tendrils (cirrhi) of conidia can be seen extruding in wet weather.[28]

Environment and disease cycle

The primary plant tissues targeted by C. parasitica are the inner bark, an area containing the conductive tissue, and the cambium, a layer of actively dividing cells that give rise to secondary vascular tissues. In these tissues, the pathogen forms diffuse cankers in which the mycelium overwinters.[29] In the following spring, two types of fruiting bodies will form: pycnidia, usually first, and perithecia.[29] Following rainfall, the pycnidia ooze orange tendrils of conidia, the asexual spores, while perithecia forcibly eject ascospores, the sexual spores.[29][30] Upon becoming airborne, ascospores are carried by eddies of wind to new hosts or infect other parts of the same tree.[29] When insects, birds, or other wild life come into contact with the cankers, they can mechanically disperse the conidia to a new host.[29][30] Additionally, the asexual spores can be dispersed by rain splash.[30] Once on the new host, or new area of the tree, the spores can germinate and infect the inner bark through insect wounds and fissures in the outer bark.

If cankers continue to form and expand, the fungus can girdle the stem, severing the flow of nutrients and water to the vital vegetative tissues. The absence of nutrient dispersal will result in tree death; however, the root system will survive. As a result, American chestnuts exist mainly as shrubs sprouting from the old, surviving roots.[30] These sprouts usually succumb to infection by C. parasitica before reaching sexual maturity.

Management: hypovirulence, sanitation, and chemical control

In Europe during the late 1960s, it was found that a strain of C. parasitica was less virulent, only able to produce shallow cankers that the tree could eventually form callus tissue over. The trait of hypovirulence could be transferred from an avirulent strain to a lethal strain through anastomosis, the fusion of hyphae.[31] It was later discovered that this attenuated virulence was due to infection by a dsRNA mycovirus, Cryphonectria hypovirus 1 (CHV1).[32]

Considering the nature of hypovirulent strains, there has been a strong interest to use them to manage lethal C. parasitica strains. In Europe, natural dissemination of hypovirulence in pathogen populations resulted in the restoration of economically valuable chestnuts.[31] Unfortunately, this was not the case in the United States. Unlike Europe, the US has a greater diversity of C. parasitica strains.[33] Thus, the spread of the mycovirus in American C. parasitica populations are inhibited by vegetative incompatibility, an allorecognition system that inhibits the fusion of hyphae between individuals that are genetically distinct at specific loci.[32][31] Recently, however, "super mycovirus donor strains" of C. parasitica have been engineered to overcome this incompatibility system and could potentially be employed as a method of biological control.[34]

In addition to biocontrol, chestnut blight can also be managed by sanitation practices and chemical control; however, such management strategies are only feasible on a small scale, such as in an orchard. Sanitation practices like the pruning of symptomatic limbs and removal of infected trees can serve to eliminate sources of inoculum and limit the spread of the pathogen.[29] Additionally, some fungicides have been shown to be effective at controlling disease. In a study on the chemical control of chestnut blight in Castanea sativa, it was found that the external application of both copper oxychloride and carbendazim could reduce the rate of disease by almost 50%.

Conservation efforts in North America

There are approximately 2,500 chestnut trees growing on 60 acres near West Salem, Wisconsin, which is the world's largest remaining stand of American chestnut. These trees are the descendants of those planted by Martin Hicks, an early settler in the area. In the late 1800s, Hicks planted fewer than a dozen chestnuts. Planted outside the natural range of American chestnut, these trees escaped the initial wave of infection by chestnut blight, but in 1987, scientists found blight also in this stand. Scientists are working to try to save the trees. There is a program to bring American chestnut back to the Eastern forest funded by the American Chestnut Foundation, Wisconsin Department of Natural Resources, USDA Forest Service, West Virginia University, Michigan State University, and Cornell University.[35]

Removing blighted trees to control the disease was first attempted when the blight was discovered, but this proved to be an ineffective solution. Scientists then set out to introduce a hyperparasitic hypovirus into the chestnut blight fungus. The trees infected with virus-treated fungus responded immediately and began to heal over their cankers. However, the virus was so efficient at attenuating fungal growth that it prevented spreading of the virus from an infected fungus growing on one tree to that growing on another tree. Only the virus-treated trees recovered. Scientific opinion regarding the future of the stand varies.[35]

Hybrid chestnut trees

Current efforts are underway by the Forest Health Initiative to use modern breeding techniques and genetic engineering to create resistant tree strains, with contributions from SUNY College of Environmental Science and Forestry, Penn State, the University of Georgia, and the US Forest Service. One of the most successful methods of breeding is to create a back cross of a resistant species (such as one from China or Japan) and American chestnut. Researchers identified two or three genes that allow for blight resistance, and are focusing on giving the American chestnut hybrids only those genes from the Chinese or Japanese chestnut.[36]

The two species are first bred to create a 50/50 hybrid. After three back crosses with American chestnut, the remaining genome is approximate 1/16 that of the resistant tree, and 15/16 American. The strategy is to select blight-resistance genes during the back crossing, while preserving the more wild-type traits of American chestnut as the dominant phenotype. Thus, the newly bred hybrid chestnut trees should reach the same heights as the original American chestnut. Many of these 15/16 American chestnut hybrids have been planted along the East Coast, including in the Jefferson National Forest and on the Flight 93 National Memorial. Some of these sites have had researchers check on the saplings that have been planted to see their survival rate. For the hybrids to do well, they need areas with decent drainage and abundant sunlight.[37] Meeting these needs can be hard to do, so not all restoration areas have been successful with hybrid survival.

Transgenic blight-resistant chestnut trees

Plant pathologists, Drs. William Powell and Charles Maynard, working at the State University of New York College of Environmental Science and Forestry, have developed American chestnuts which have full blight resistance. Full resistance was attained by introducing a wheat gene coding for the enzyme oxalate oxidase into the American chestnut genome. This enzyme breaks down the oxalic acid secreted by the fungus into carbon dioxide and hydrogen peroxide. Early studies on hypovirulence showed that less virulent strains of the chestnut blight produced less oxalic acid when attacking the cambium.[38] The transgenic trees have blight resistance either equal to or surpassing that of Chinese chestnuts.[39] In 2013, SUNY ESF had over 100 individual events being tested, with more than 400 slated to be in the field or in the lab for various assay tests in the next several years and more than 1,000 trees growing in several field sites in 2014.[40] Government approval will be required before returning any of these blight resistant trees to the wild.[41] The New York Botanical Garden has planted several of the transgenic trees for public display.[42]

Economic and ecological impact of disease

In less than fifty years after its emergence, C. parastica virtually eliminated American chestnut as a canopy species in 8.8 million acres (3.6×10^6 ha) acres of forest.[43] The chestnut fruit was a major food source for animals in the low elevation Appalachian forests. This loss resulted in a drastic decrease of the squirrel population, the extinction of seven native moth species, and the slowed recovery of deer, Cooper's hawk, cougar, and bobcat populations.[44] The effects of this disease also rippled further through the ecosystem, being linked to a decrease in the abundance of cavity-nesting birds and to a decrease in river water quality which negatively affected aquatic invertebrate populations.[43]

In 1912, standing chestnut timber in just three states was estimated to be $82.5 million ($1.9 billion in current dollars) in value.[43] Therefore, in addition to ecological impacts, C. parasitica potentially caused a devastating loss in economic welfare for communities dependent on the chestnut tree. Mountaineers, residents of Appalachian Mountain communities, had to drastically alter their life styles to cope with the effects of this disease.[44]

Economic effects have also been considerable in Europe, particularly before CHV1 spreads naturally to a region. In Greece for example, the disease forced the migration of people who could not longer afford to live off chestnut trees. It has also led to a 40% decline in Greek chestnut production.[45]

See also

References
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Roane, M. K., Griffin, G .J. & Elkins, J. K. 1986. Chestnut Blight, Other Endothia Diseases, and the Genus Endothia. American Phytopathological Society, APS Press, St. Paul.

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