Coefficient of coincidence

In genetics, the coefficient of coincidence (c.o.c.) is a measure of interference in the formation of chromosomal crossovers during meiosis. It is generally the case that, if there is a crossover at one spot on a chromosome, this decreases the likelihood of a crossover in a nearby spot.[1] This is called interference.

DNA segment with three genes, showing a double recombination event. If the individual recombination rates (between A and B; and between B and C) are known, then the c.o.c. between the regions AB and BC can be calculated from the rate of double recombination.

The coefficient of coincidence is typically calculated from recombination rates between three genes. If there are three genes in the order A B C, then we can determine how closely linked they are by frequency of recombination. Knowing the recombination rate between A and B and the recombination rate between B and C, we would naively expect the double recombination rate to be the product of these two rates.

The coefficient of coincidence is calculated by dividing the actual frequency of double recombinants by this expected frequency:[1]

c.o.c. = actual double recombinant frequency / expected double recombinant frequency

Interference is then defined as follows:[1]

interference = 1 − c.o.c.

This figure tells us how strongly a crossover in one of the DNA regions (AB or BC) interferes with the formation of a crossover in the other region.

Worked example

Drosophila females of genotype a+a b+b c+c were crossed with males of genotype aa bb cc. This led to 1000 progeny of the following phenotypes:

a+b+c+: 244 (parental genotype, shows no recombination)
a+b+c: 81 (recombinant between B and C)
a+bc+: 23 (double recombinant)
a+bc: 152 (recombinant between A and B)
ab+c+: 148 (recombinant between A and B)
ab+c: 27 (double recombinant)
abc+: 89 (recombinant between B and C)
abc: 236 (parental genotype, shows no recombination)

From these numbers it is clear that the b+/b locus lies between the a+/a locus and the c+/c locus.

There are 23 + 152 + 148 + 27 = 350 progeny showing recombination between genes A and B. And there are 81 + 23 + 27 + 89 = 220 progeny showing recombination between genes B and C. Thus the expected rate of double recombination is (350 / 1000) * (220 / 1000) = 0.077, or 77 per 1000.

However, there are actually only 23 + 27 = 50 double recombinants. The coefficient of coincidence is therefore 50 / 77 = 0.65.

Interference is 1 − 0.65 = 0.35.

References
  1. Griffiths AJF, Wessler SR, Lewontin RC, Carroll SB (2008) Introduction to Genetic Analysis. New York: W. H. Freeman and Company.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.