Dio Brando

In Phantom Blood, the child of an abusive drunkard named Dario Brando, Dio Brando becomes the adopted brother of Jonathan Joestar by using the promise that George Joestar made to Dario years ago. Upon entering the Joestar estate after his father died, Dio wasted no time unequivocally making young Jonathan's life a living hell to take everything from him including abusing Jonathan's dog. Dio underestimated Jonathan's retaliation for forcefully kissing his beloved Erina Pendleton, spending the next seven years feigning to be Jonathan's friend before enacting his plan to steal the Joestar fortune by poisoning George with the same poison he used to kill Dario. When Jonathan investigates the matter for proof, Dio plots to use an ancient Aztec stone mask to murder Jonathan.

However, Dio learns the mask transforms the wearer into a vampire when exposed to blood, and uses it on himself when his scheme is exposed. Although seemingly killed when Jonathan impales him on a stone statue and leaves him in the burning Joestar mansion, Dio survives, and proceeds to start his subjugation of England in the small village of Windknight's Lot, amassing sired followers to his cause. Jonathan is sought out by William Zeppeli, a master of an ancient martial art called Hamon, which can kill Vampires, and he teaches Jonathan how to use this ability as Dio sends other vampires and zombies in his pursuit to kill him so he can take over the world.

Jonathan seemingly kills him once more, but Dio's head survives. With the help of his servant, Wang Chan, Dio attacks Jonathan on his honeymoon with Erina in an attempt to claim his enemy's body for his own. Despite having his throat fatally punctured by Dio, Jonathan is able to use Hamon one last time to overload the ship's engines, with Dio apparently destroyed in the resulting explosion. Erina, escaping unharmed with an orphaned girl, survives with their unborn son.

Dio Brando does not appear in Battle Tendency, but the aftermath of his apparent death at the hands of Jonathan Joestar affects the plot. It is ultimately revealed that Jonathan and Erina's son George Joestar II, a famed pilot of the Royal Air Force in World War I, was murdered by one of Dio's zombie henchmen who survived the battle of Knight's Lot and had infiltrated the RAF. George II's wife, the orphaned girl Elizabeth, hunted down this zombie and killed him with her Hamon. But as no one in the RAF knew that her victim was not human, she became a wanted murderer, Elizabeth was forced to go into hiding as Lisa Lisa with the help of the Speedwagon Foundation. Lisa Lisa would later encounter her son Joseph Joestar, teaching him Hamon so he can defeat the Pillar Man, Kars, whose creation of the Stone masks made him indirectly responsible for Dio's transformation.

It is revealed in Stardust Crusaders that Dio survived the ship's explosion and attached his head to Jonathan's headless body as the ship sank, confining himself in a casket at the bottom of the ocean for a century until a shipwreck salvage operation unintentionally retrieved him in 1983. Now known mononymously as "DIO", he soon meets the mystic Enya Geil who awakens his Stand, The World (世界（ザ・ワールド）, Za Wārudo), allowing him to stop the passage of time around him. As his head is attached to Jonathan Joestar's body, Dio's acquisition of The World also awakens Stand abilities in all of Jonathan's living descendants. While Joseph Joestar and his grandson Jotaro Kujo have the resolve to control their Stands, Joseph's daughter and Jotaro's mother Holly has her very life sapped away from her own Stand because of her kind disposition. This spurs Jotaro and Joseph to track down Dio along with their friends Mohammed Avdol, Noriaki Kakyoin, Jean-Pierre Polnareff, and Iggy the Boston Terrier to kill him once and for all, freeing Holly from the curse the Stand has trapped her under.

Dio responds by preemptively trying to assassinate the Joestars before they can find him in his lair in Cairo, sending other evil Stand users, some of whom he controlled mentally before they joined the Joestars on their journey. Along the way, Avdol and Iggy are killed by Dio's right-hand man Vanilla Ice, who in turn is killed by Polnareff. Dio kills Jotaro's friend Kakyoin in combat and temporarily kills Joseph after draining him of his blood, but Jotaro's own Stand Star Platinum proves to be The World's equally powered counterpart, and Jotaro's own rage at Dio being the cause of his friends' deaths and his mother's dwindling health leads him to kill Dio with Star Platinum. After the Speedwagon Foundation revives Joseph via blood transfusion from Jonathan's corpse, he and Jotaro take their ancestor's corpse out into the desert and expose it to sunlight, vanquishing Dio once and for all.

Dio is not present in either Diamond Is Unbreakable or Golden Wind, but his influence on the story is still present. In Diamond Is Unbreakable, it is revealed that Dio acquired his Stand by means of an ancient artifact known as the "Bow and Arrow", which has reappeared in the fictional Japanese town of Morioh because the children of one of Dio's former lackeys, Mr. Nijimura, are seeking out someone who can possibly free their father from the strange mutation he has suffered as a result of Dio's death. In Golden Wind, the protagonist Giorno Giovanna is Dio's son, but because Dio was in possession of Jonathan's body at the time of Giorno's conception, Giorno is biologically Jonathan's son and thus a member of the Joestar family, and his own virtuous soul prevents him from becoming evil as Dio was, thanks to his encounter with a heroic gangster in his childhood life.

Dio's influence on the JoJo story returns in Stone Ocean, where it is revealed that he met the story arc's main antagonist, Father Enrico Pucci, during the events of Stardust Crusaders. He told Father Pucci of his desire to use his Stand The World in order to reach "Heaven", and saw in the young priest a friendship that would help him achieve his goals. Father Pucci's reverence for Dio drives him to carry out his plans decades later, manipulating events to frame Jotaro Kujo's daughter Jolyne Cujoh for murder in order to trap her in the prison where he serves as a chaplain, using the final physical remains of Dio to evolve his Stand to reset reality in what he believes is Dio's vision of Heaven, a world where everyone knows their own destiny, and more importantly a world free of the Joestar bloodline. However, his hubris leads to his defeat at the hands of Jolyne's last surviving friend Emporio, who manages to reset reality back to what it once was, but not quite exactly the way it was before, as, in this new version of the world, Pucci is missing instead of the Joestars.

Dio Brando himself does not appear in Steel Ball Run, set in an alternate universe in 1890, but an alternate version of himself known as Diego Brando does. Diego Brando is, like his original counterpart, the child of an abusive household, and one who reveres his mother. She instilled in him a sense of pride that he took with him as he became a masterful jockey in his adulthood. When he decides to take part in the Steel Ball Run horse race across the United States, he winds up under the thrall of the Stand Scary Monsters (スケアリーモンスターズ, Sukearī Monsutāzu), transforming him into a dromaeosaurid dinosaur. As a result of this encounter, however, he gains his own Stand Scary Monsters, which grants him the same abilities that he now has under his control.

Diego is hired by United States President Funny Valentine in his quest to seek out the pieces of the Saint's Corpse scattered along the racecourse, but after some time fighting against the heroes Johnny Joestar and Gyro Zeppeli, he realizes that President Valentine's plans are more dangerous to the world as a whole and joins the heroes in an attempt to stop him, only to die during his attempt to kill the evil President.

Valentine then uses his Stand's powers to summon a Diego Brando from an alternate universe. This Diego Brando's possesses the same stand powers as Dio, thanks to having his own version of The World as a Stand. He manages to defeat Johnny Joestar in battle and wins the Steel Ball Run, in addition to obtaining all of the Saint's Corpse and hiding it in a secure location, even though President Valentine has already been killed by Johnny in a fight. However, in this alternate universe Diego meets his demise at the hands of Lucy Steel, who uses the skull of this universe's Diego Brando to obliterate his own head as a result of the effects of Valentine's Stand.

Dio makes a brief Cameo during the events of the "The Wonder of You (The Miracle of Your Love)" arc, along with the Stone Mask.

Dio Brando has appeared in many video games related to the JoJo's Bizarre Adventure franchise, usually as a player character or a boss. Some games include multiple versions of the character; in JoJo's Bizarre Adventure: Eyes of Heaven for example, both Dio Brando from Part 1 and Dio from Part 3 are playable characters, along with Diego Brando and Parallel World Diego from Part 7. Furthermore, he has appeared as a playable character in several crossover video games, such as Jump Super Stars, Jump Ultimate Stars, and Jump Force.

Dio is the main antagonist in the story mode of JoJo's Bizarre Adventure: Eyes of Heaven. Although the video game begins with the final scenes of Stardust Crusaders, the antagonist is a version of Dio from another universe who managed to beat the Joestar family. He seeks to control all universes by means of the Saint's Corpse, and uses his more advanced powers over time to bring all of the fallen heroes and villains back to life to serve him in preventing Jotaro Kujo and the other Joestars from obtaining all of the Saint's Corpse, or at least using them to gather the parts so he can steal them from them and achieve his ultimate power. This version of Dio is called "Dio, Gone to Heaven" (天国に到達したDIO, Tengoku ni Tōtatsu-shita Dio) and his Stand is called "The World Over Heaven" (ザ・ワールド・オーバーヘブン, Za Wārudo Ōbā Hebun).

Dio's Part 7 counterpart, Diego Brando, first encounters Scary Monsters when he comes into conflict with its original user Dr. Ferdinand, Ferdinand "infects" Diego, as well as an entire village, transforming them into dinosaurs. After gaining the Left Eye Corpse Part, Diego gains his own version of Scary Monsters that allows him to transform into a dinosaur, partially or entirely. Scary Monsters grants Diego increased strength, speed, and "dynamic vision", and retains the infection ability.

After Diego's death by the hands of Funny Valentine, Valentine summons a Diego from an alternate universe. This Diego possesses The World like Part 3's Dio, and can also stop time. The design of Diego's The World is very similar to that of Part 3, apart from its skinnier profile and the replacement of the hearts on The World’s knees for capital Ds.