Folin's reagent

Folin's reagent or sodium 1,2-naphthoquinone-4-sulfonate is a chemical reagent used as a derivatizing agent to measure levels of amines and amino acids.[1] The reagent reacts with them in alkaline solution to produce a fluorescent material that can be easily detected.[2]

Folin's reagent
Names
IUPAC name
Sodium 3,4-dioxo-3,4-dihydronaphthalene-1-sulfonate
Identifiers
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.007.555
EC Number
  • 208-308-9
UNII
Properties
C10H5NaO5S
Molar mass 260.20 g/mol
Hazards
Safety data sheet Oxford MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

This should not be confused with Folin-Ciocalteu reagent, that is used to detect phenolic compounds.

The Folin reagent can be used with an acidic secondary reagent to distinguish MDMA and related compounds from PMMA and related compounds.[3]

Substance Reaction
MDMA Pink[3]
MDE No Reaction[3]
MDA Yellow[3]
BZP Orange to red[3]
TFMPP Pinkish red[3]
Methylone No reaction[3]
Methamphetamine Pink[3]
Amphetamine Light orange[3]
PMA Light Yellow[3]
PMMA Light orange[3]
Ketamine No reaction[3]
Mescaline No reaction[3]
Cocaine No reaction[3]
Aspirin No reaction[3]
Sugar No reaction[3]

See also

References

  1. Saurina J, Hernández-Cassou S (1994). "Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate". Journal of Chromatography A. 676 (2): 311–9. doi:10.1016/0021-9673(94)80431-1. PMID 7921184.
  2. Kobayashi Y, Kubo H, Kinoshita T (1987). "Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography". Anal. Biochem. 160 (2): 392–8. doi:10.1016/0003-2697(87)90066-2. PMID 3578768.
  3. "Folin Reagent Testing Kit". Dancesafe. June 2015. Archived from the original on 11 September 2015. Retrieved 8 August 2015.
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