Gibbs–Donnan effect

The Gibbs–Donnan effect (also known as the Donnan's effect, Donnan law, Donnan equilibrium, or Gibbs–Donnan equilibrium) is a name for the behaviour of charged particles near a semi-permeable membrane that sometimes fail to distribute evenly across the two sides of the membrane.[1] The usual cause is the presence of a different charged substance that is unable to pass through the membrane and thus creates an uneven electrical charge.[2] For example, the large anionic proteins in blood plasma are not permeable to capillary walls. Because small cations are attracted, but are not bound to the proteins, small anions will cross capillary walls away from the anionic proteins more readily than small cations.

Donnan equilibrium across a cell membrane (schematic)

Thus, some ionic species can pass through the barrier while others cannot. The solutions may be gels or colloids as well as solutions of electrolytes, and as such the phase boundary between gels, or a gel and a liquid, can also act as a selective barrier. The electric potential arising between two such solutions is called the Donnan potential.

The effect is named after the American physicist Josiah Willard Gibbs who proposed it in 1878 and the British chemist Frederick G. Donnan who studied it experimentally in 1911.[3]

The Donnan equilibrium is prominent in the triphasic model for articular cartilage proposed by Mow and Lai, as well as in electrochemical fuel cells and dialysis.

The Donnan effect is tactic pressure attributable to cations (Na+ and K+) attached to dissolved plasma proteins.

Example

The presence of a charged impermeant ion (for example, a protein) on one side of a membrane will result in an asymmetric distribution of permeant charged ions. The Gibbs–Donnan equation at equilibrium states (assuming permeant ions are Na+ and Cl−):

Equivalently,

StartEquilibrium Osmolarity
: 9 Na, 9 Cl
: 9 Na, 9 Protein		: 6 Na, 6 Cl
: 12 Na, 3 Cl, 9 Protein		 : 12
: 24

Double Donnan

Note that Sides 1 and 2 are no longer in osmotic equilibrium (i.e. the total osmolytes on each side are not the same)

In vivo, ion balance does equilibriate at the proportions that would be predicted by the Gibbs–Donnan model, because the cell cannot tolerate the attendant large influx of water. This is balanced by instating a functionally impermeant cation, Na+, extracellularly to counter the anionic protein. Na+ does cross the membrane via leak channels (the permeability is approximately 1/10 that of K+, the most permeant ion) but, as per the pump-leak model, it is extruded by the Na+/K+-ATPase.[4]

pH change

Because there is a difference in concentration of ions on either side of the membrane, the pH may also differ when protons are involved. In many instances, from ultrafiltration of proteins to ion exchange chromatography, the pH of the buffer adjacent to the charged groups of the membrane is different from the pH of the rest of the buffer solution.[5] When the charged groups are negative (basic), then they will attract protons so that the pH will be lower than the surrounding buffer. When the charged groups are positive (acidic), then they will repel protons so that the pH will be higher than the surrounding buffer.

Red blood cells

When tissue cells are in a protein-containing fluid, the Donnan effect of the cytoplasmic proteins is equal and opposite to the Donnan effect of the extracellular proteins. The opposing Donnan effects cause chloride ions to migrate inside the cell, increasing the intracellular chloride concentration. The Donnan effect may explain why some red blood cells do not have active sodium pumps; the effect relieves the osmotic pressure of plasma proteins, which is why sodium pumping is less important for maintaining the cell volume .[6]

Neurology

Brain tissue swelling, known as cerebral oedema, results from brain injury and other traumatic head injuries that can increase intracranial pressure (ICP). Negatively charged molecules within cells create a fixed charge density, which increases intracranial pressure through the Donnan effect. ATP pumps maintain a negative membrane potential even though negative charges leak across the membrane; this action establishes a chemical and electrical gradient.[7]

The negative charge in the cell and ions outside the cell creates a thermodynamic potential; if damage occurs to the brain and cells lose their membrane integrity, ions will rush into the cell to balance chemical and electrical gradients that were previously established. The membrane voltage will become zero, but the chemical gradient will still exist. To neutralize the negative charges within the cell, cations flow in, which increases the osmotic pressure inside relative to the outside of the cell. The increased osmotic pressure forces water to flow into the cell and tissue swelling occurs.[8]

See also

References
  1. "Gibbs–Donnan effect". Archived from the original on 2007-06-18. Retrieved 2006-08-28.
  2. The Gibbs–Donnan Equilibrium..., D.C. Mikulecky, retrieved 28 August 2006
  3. Donnan, F. G. (1911). "Theorie der Membrangleichgewichte und Membranpotentiale bei Vorhandensein von nicht dialysierenden Elektrolyten. Ein Beitrag zur physikalisch-chemischen Physiologie" [The theory of membrane equilibrium and membrane potential in the presence of a non-dialyzable electrolyte. A contribution to physical-chemical physiology]. Zeitschrift für Elektrochemie und Angewandte Physikalische Chemie. 17 (10): 572–581. doi:10.1002/bbpc.19110171405 (inactive 2021-01-14).CS1 maint: DOI inactive as of January 2021 (link)
  4. Leaf, Alexander (1959). "Maintenance of Concentration Gradients and Regulation of Cell Volume". Annals of the New York Academy of Sciences. 72 (12): 396–404. Bibcode:1959NYASA..72..396L. doi:10.1111/j.1749-6632.1959.tb44168.x. PMID 13627925. S2CID 44583830.
  5. Bolton, Glen R.; Boesch, Austin W.; Basha, Jonida; LaCasse, Daniel P.; Kelley, Brian D.; Acharya, Hari (2011-01-01). "Effect of protein and solution properties on the donnan effect during the ultrafiltration of proteins". Biotechnology Progress. 27 (1): 140–152. doi:10.1002/btpr.523. ISSN 1520-6033. PMID 21312362. S2CID 5931842.
  6. Kurbel, S. (2011). Donnan effect on chloride ion distribution as a determinant of body fluid composition that allows action potentials to spread via fast sodium channels. Theoretical Biology & Medical Modelling, 8, 16. http://doi.org/10.1186/1742-4682-8-16
  7. Elkin, Benjamin S.; Shaik, Mohammed A.; Morrison, Barclay (13 February 2010). "Fixed negative charge and the Donnan effect: a description of the driving forces associated with brain tissue swelling and oedema". Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences. 368 (1912): 585–603. Bibcode:2010RSPTA.368..585E. doi:10.1098/rsta.2009.0223. PMC 2944388. PMID 20047940.
  8. Elkin, B. S., Shaik, M. A., & Morrison, B. (2010). Fixed negative charge and the Donnan effect: a description of the driving forces associated with brain tissue swelling and oedema. Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences, 368(1912), 585–603. http://doi.org/10.1098/rsta.2009.0223
  • IUPAC Compendium of Chemical Terminology 2nd Edition (1997)
  • Van C. Mow Basic orthopaedic biomechanics and mechano-biology, 2nd Ed. Lippincott Williams & Wilkins, Philadelphia, 2005
  • Mapleson W. W. "Computation of the effect of Donnan equilibrium on pH in equilibrium dialysis". Journal of Pharmacological Methods, May 1987.
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