Glycoside hydrolase family 3
In molecular biology, glycoside hydrolase family 3 is a family of glycoside hydrolases.
Glycosyl hydrolase family 3 N terminal domain | |||||||||
---|---|---|---|---|---|---|---|---|---|
Identifiers | |||||||||
Symbol | Glyco_hydro_3 | ||||||||
Pfam | PF00933 | ||||||||
Pfam clan | CL0058 | ||||||||
InterPro | IPR001764 | ||||||||
PROSITE | PDOC00621 | ||||||||
SCOP2 | 1ex1 / SCOPe / SUPFAM | ||||||||
CAZy | GH3 | ||||||||
|
Glycosyl hydrolase family 3 C-terminal domain | |||||||||
---|---|---|---|---|---|---|---|---|---|
![]() crystal structure of barley beta-d-glucan glucohydrolase isoenzyme exo1 | |||||||||
Identifiers | |||||||||
Symbol | Glyco_hydro_3_C | ||||||||
Pfam | PF01915 | ||||||||
InterPro | IPR002772 | ||||||||
PROSITE | PDOC00621 | ||||||||
SCOP2 | 1ex1 / SCOPe / SUPFAM | ||||||||
|
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of over 100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]
Glycoside hydrolase family 3 CAZY GH_3 comprises enzymes with a number of known activities; beta-glucosidase (EC 3.2.1.21); beta-xylosidase (EC 3.2.1.37); N-acetyl beta-glucosaminidase (EC 3.2.1.52); glucan beta-1,3-glucosidase (EC 3.2.1.58); cellodextrinase (EC 3.2.1.74); exo-1,3-1,4-glucanase (EC 3.2.1). These enzymes are two-domain globular proteins that are N-glycosylated at three sites.[8]
External links
References
- Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. Bibcode:1995PNAS...92.7090H. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
- Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 (Pt 2): 695–6. doi:10.1042/bj3160695. PMC 1217404. PMID 8687420.
- "Home". CAZy.org. Retrieved 2018-03-06.
- Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490–5. doi:10.1093/nar/gkt1178. PMC 3965031. PMID 24270786.
- "Glycoside Hydrolase Family 3". CAZypedia.org. Retrieved 2018-03-06.
- CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes" (PDF). Glycobiology. 28 (1): 3–8. doi:10.1093/glycob/cwx089. PMID 29040563.
- Varghese JN, Hrmova M, Fincher GB (February 1999). "Three-dimensional structure of a barley beta-D-glucan exohydrolase, a family 3 glycosyl hydrolase". Structure. 7 (2): 179–90. doi:10.1016/S0969-2126(99)80024-0. PMID 10368285.