MYO10
Myosin X, also known as MYO10, is a protein that in humans is encoded by the MYO10 gene.[5][6][7]
Myosin X is an actin-based motor protein known to associate at the tips of filopodia.[8]
Model organisms
Model organisms have been used in the study of MYO10 function. A conditional knockout mouse line called Myo10tm2(KOMP)Wtsi was generated at the Wellcome Trust Sanger Institute.[9] Male and female animals underwent a standardized phenotypic screen[10] to determine the effects of deletion.[11][12][13][14] Additional screens performed: - In-depth immunological phenotyping[15]
| Characteristic | Phenotype |
|---|---|
| All data available at.[10][15] | |
| Peripheral blood leukocytes 6 Weeks | Abnormal |
| Haematology 6 Weeks | Normal |
| Insulin | Normal |
| Homozygous viability at P14 | Normal |
| Homozygous Fertility | Normal |
| General Observations | Abnormal |
| Body weight | Normal |
| Neurological assessment | Normal |
| Grip strength | Normal |
| Dysmorphology | Abnormal |
| Indirect calorimetry | Normal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Normal |
| Radiography | Normal |
| Eye morphology | Abnormal |
| Clinical chemistry | Normal |
| Haematology 16 Weeks | Normal |
| Peripheral blood leukocytes 16 Weeks | Normal |
| Heart weight | Normal |
| Salmonella infection | Normal |
| Cytotoxic T Cell Function | Normal |
| Spleen Immunophenotyping | Normal |
| Mesenteric Lymph Node Immunophenotyping | Normal |
| Epidermal Immune Composition | Normal |
| Influenza Challenge | Normal |
References
- GRCh38: Ensembl release 89: ENSG00000145555 - Ensembl, May 2017
- GRCm38: Ensembl release 89: ENSMUSG00000022272 - Ensembl, May 2017
- "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- "Entrez Gene: MYO10 myosin X".
- Bement WM, Hasson T, Wirth JA, Cheney RE, Mooseker MS (Jul 1994). "Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types". Proceedings of the National Academy of Sciences of the United States of America. 91 (14): 6549–53. doi:10.1073/pnas.91.14.6549. PMC 44240. PMID 8022818.
- Hasson T, Skowron JF, Gilbert DJ, Avraham KB, Perry WL, Bement WM, Anderson BL, Sherr EH, Chen ZY, Greene LA, Ward DC, Corey DP, Mooseker MS, Copeland NG, Jenkins NA (Sep 1996). "Mapping of unconventional myosins in mouse and human". Genomics. 36 (3): 431–9. doi:10.1006/geno.1996.0488. PMID 8884266.
- Berg JS, Cheney RE (Mar 2002). "Myosin-X is an unconventional myosin that undergoes intrafilopodial motility". Nature Cell Biology. 4 (3): 246–50. doi:10.1038/ncb762. PMID 11854753. S2CID 22683743.
- Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
- "International Mouse Phenotyping Consortium".
- Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
- White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
- "Infection and Immunity Immunophenotyping (3i) Consortium".
Further reading
- Hasson T, Skowron JF, Gilbert DJ, Avraham KB, Perry WL, Bement WM, Anderson BL, Sherr EH, Chen ZY, Greene LA, Ward DC, Corey DP, Mooseker MS, Copeland NG, Jenkins NA (Sep 1996). "Mapping of unconventional myosins in mouse and human". Genomics. 36 (3): 431–9. doi:10.1006/geno.1996.0488. PMID 8884266.
- Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Oct 1998). "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 5 (5): 277–86. doi:10.1093/dnares/5.5.277. PMID 9872452.
- Rojas K, Serrano de la Peña L, Gallardo T, Simmons A, Nyce K, McGrath R, Considine E, Vasko AJ, Peterson E, Grady D, Cox R, Andrew LJ, Lovett M, Overhauser J, Williams CJ (Dec 1999). "Physical map and characterization of transcripts in the candidate interval for familial chondrocalcinosis at chromosome 5p15.1". Genomics. 62 (2): 177–83. doi:10.1006/geno.1999.5997. PMID 10610710.
- Berg JS, Derfler BH, Pennisi CM, Corey DP, Cheney RE (Oct 2000). "Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin". Journal of Cell Science. 113. 113 Pt 19: 3439–51. PMID 10984435.
- Rogers MS, Strehler EE (Apr 2001). "The tumor-sensitive calmodulin-like protein is a specific light chain of human unconventional myosin X". The Journal of Biological Chemistry. 276 (15): 12182–9. doi:10.1074/jbc.M010056200. PMID 11278607.
- Cox D, Berg JS, Cammer M, Chinegwundoh JO, Dale BM, Cheney RE, Greenberg S (Jul 2002). "Myosin X is a downstream effector of PI(3)K during phagocytosis". Nature Cell Biology. 4 (7): 469–77. doi:10.1038/ncb805. PMID 12055636. S2CID 24127183.
- Navarro-Lérida I, Martínez Moreno M, Roncal F, Gavilanes F, Albar JP, Rodríguez-Crespo I (Feb 2004). "Proteomic identification of brain proteins that interact with dynein light chain LC8". Proteomics. 4 (2): 339–46. doi:10.1002/pmic.200300528. PMID 14760703. S2CID 8868600.
- Zhang H, Berg JS, Li Z, Wang Y, Lång P, Sousa AD, Bhaskar A, Cheney RE, Strömblad S (Jun 2004). "Myosin-X provides a motor-based link between integrins and the cytoskeleton". Nature Cell Biology. 6 (6): 523–31. doi:10.1038/ncb1136. PMID 15156152. S2CID 24260820.
- Tokuo H, Ikebe M (Jun 2004). "Myosin X transports Mena/VASP to the tip of filopodia". Biochemical and Biophysical Research Communications. 319 (1): 214–20. doi:10.1016/j.bbrc.2004.04.167. PMID 15158464.
- Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
- Sousa AD, Berg JS, Robertson BW, Meeker RB, Cheney RE (Jan 2006). "Myo10 in brain: developmental regulation, identification of a headless isoform and dynamics in neurons". Journal of Cell Science. 119 (Pt 1): 184–94. doi:10.1242/jcs.02726. PMID 16371656.
- Bohil AB, Robertson BW, Cheney RE (Aug 2006). "Myosin-X is a molecular motor that functions in filopodia formation". Proceedings of the National Academy of Sciences of the United States of America. 103 (33): 12411–6. doi:10.1073/pnas.0602443103. PMC 1567893. PMID 16894163.
- Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
External links
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