NPLOC4

NPLOC4
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2PJH
Identifiers
Aliases	NPLOC4, NPL4, NPL4 homolog, ubiquitin recognition factor
External IDs	OMIM: 606590 MGI: 2679787 HomoloGene: 5403 GeneCards: NPLOC4
Gene location (Human)
Chr.	Chromosome 17 (human)[1]
End	81,648,465 bp[1]
Gene location (Mouse)
Chr.	Chromosome 11 (mouse)[2]
End	120,437,708 bp[2]
RNA expression pattern
	More reference expression data
Gene ontology
Molecular function	• ubiquitin binding; • GO:0001948 protein binding; • metal ion binding; • ubiquitin protein ligase binding;
Cellular component	• cytoplasm; • cytosol; • nuclear outer membrane-endoplasmic reticulum membrane network; • endoplasmic reticulum; • cell nucleus; • UFD1-NPL4 complex; • nucleoplasm; • VCP-NPL4-UFD1 AAA ATPase complex;
Biological process	• error-free translesion synthesis; • retrograde protein transport, ER to cytosol; • Golgi organization; • ubiquitin-dependent ERAD pathway; • proteasome-mediated ubiquitin-dependent protein catabolic process; • negative regulation of type I interferon production; • negative regulation of RIG-I signaling pathway; • ubiquitin-dependent protein catabolic process;
	Sources:Amigo / QuickGO
Orthologs
	55666
	217365
	ENSG00000182446
	ENSMUSG00000039703
	Q8TAT6
	P60670
	NM_017921; NM_001369698
	NM_001195023; NM_199469
	NP_060391; NP_001356627; NP_060391.2
	NP_001181952; NP_955763
	Wikidata
View/Edit Human	View/Edit Mouse

Nuclear protein localization protein 4 homolog is a protein that in humans is encoded by the NPLOC4 gene.[5][6][7]

Model organisms

*Nploc4* knockout mouse phenotype
Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Salmonella infection	Normal[8]
Citrobacter infection	Normal[9]
All tests and analysis from[10][11]

Model organisms have been used in the study of NPLOC4 function. A conditional knockout mouse line, called Nploc4^{tm1a(EUCOMM)Wtsi}[12][13] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[14][15][16]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[10][17] Twenty six tests were carried out and two phenotypes were reported. No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; no significant abnormalities were observed in these animals.[10]

Interactions

NPLOC4 has been shown to interact with UFD1L.[5][18]

References

GRCh38: Ensembl release 89: ENSG00000182446 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000039703 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Botta A, Tandoi C, Fini G, Calabrese G, Dallapiccola B, Novelli G (Sep 2001). "Cloning and characterization of the gene encoding human NPL4, a protein interacting with the ubiquitin fusion-degradation protein (UFD1L)". Gene. 275 (1): 39–46. doi:10.1016/S0378-1119(01)00649-7. PMID 11574150.
Meyer HH, Shorter JG, Seemann J, Pappin D, Warren G (Jun 2000). "A complex of mammalian Ufd1 and Npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways". EMBO J. 19 (10): 2181–92. doi:10.1093/emboj/19.10.2181. PMC 384367. PMID 10811609.
"Entrez Gene: NPLOC4 nuclear protein localization 4 homolog (S. cerevisiae)".
"Salmonella infection data for Nploc4". Wellcome Trust Sanger Institute.
"Citrobacter infection data for Nploc4". Wellcome Trust Sanger Institute.
Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
Mouse Resources Portal, Wellcome Trust Sanger Institute.
"International Knockout Mouse Consortium".
"Mouse Genome Informatics".
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
Lass A, McConnell E, Fleck K, Palamarchuk A, Wójcik C (Aug 2008). "Analysis of Npl4 deletion mutants in mammalian cells unravels new Ufd1-interacting motifs and suggests a regulatory role of Npl4 in ERAD". Exp. Cell Res. 314 (14): 2715–23. doi:10.1016/j.yexcr.2008.06.008. PMID 18586029.

NPLOC4

Model organisms

Interactions

References

Further reading