Polistes exclamans

P. exclamans lives in a hierarchical society with one queen that lays the eggs. However, all of the females have the same morphology and they all have the physiological capability of taking over the egg laying if necessary (queen death, queen migration).[9][14] Since the queen has the same morphology as the rest of the workers in the colony,[16] this raises the question as to how the queen role is assigned and what makes her special. Studies have found that queens and males have higher levels of glucose, fructose and trehalose than workers, leading to higher cryoprotectant levels. P. exclamans use these sugars as cryoprotectant, which work by increasing the solute concentration in cells. This results in a greater survivability in colder weather. It was found that queens have a 76% survival rate in 5 °C (41 °F) weather compared to 17% survival of the workers over a 15-day test period. The queens also have a 0.5mm layer of fat surrounding their bodies. This allows them to live longer into the winter and possibly extend their mating season.[16] The queens seem to be the only ones capable of diapause.[17]

While there aren’t many external morphological differences between queens and workers there are some internal traits that can be signals of a wasp's social status.[17] It has also been found that caste determination is irreversibly determined by temperature during the immature stages of development. In one experiment, some wasps emerging in June were kept in long day chambers, or chambers set at 26 °C with 16 hours of light and 8 hours of darkness. These individuals had larger and more active ovaries as compared to those who were incubated in short day chambers, which are set at 22 °C with 14 hours of light and 10 hours of darkness.[17] It is thought that this phenomenon may occur because of a juvenile hormone secretion that determines the castes and a suppression of the workers’ ovaries by environmental or hormonal factors.[17]

Given that Polistes exclamans nests are often destroyed or suffer through the death of the queen (most often through natural circumstances), it is necessary and adaptive for there to be a specific system for the replacement of the queen.[18] The current system in place can best “be described as gerontocracy.” [18] Furthermore, it is also closely related to the dominance system within the colony as well. In the dominance hierarchy, older individuals are higher in rank, whereas younger individuals serve in the lower ranks.[18] These older female workers are generally more aggressive and forage more frequently.[18] In reflection of this dominance ranking and the behavior of the female workers, it is seen that when a queen is no longer present in the colony, the next eldest worker becomes the new queen.[18] This may be due to the genetics of the next eldest queen, as the replacement queen affects the overall relatedness of the females in the brood, thereby allowing the future brood to be more related to the adult workers rearing them.[18]

P. exclamans tend to have smaller nests than other wasps, with typically fewer than one hundred individuals in a given nest. Because of the size of the nest, the queen tends to be the most active individual in the nest.[19] This is because the queen has to monitor and directly control the activities of the nest. In addition to her reproductive role, the queen must also act as the pacemaker of the nest and synchronize the worker activity.[19] These last two points have recently been disputed and the workers may be self organized.[20] It has also been observed that the queen will act aggressively towards individuals that are the least active.

P. exclamans are haplodiploid insects, as are other Polistes species including Polistes metricus, Polistes dominula, and Polistes annularis. This means they have haploid males that produce identical haploid sperm, and diploid females that produce haploid eggs through meiosis. This has consequences for genetic relatedness within colonies, since sisters receive identical sperm if they share a father, and the normal half identical contribution from the mother. This results in sex-biased conflicts between the queen and the workers with respect to the sex ratio, with workers preferring a more female biased sex ratio, and queens preferring equal investment in queens and males.[21]

In Polistes exclamans, equal sex ratio is obtained when only 46.3% of investment is devoted to females as female wasps are 1.16 times larger than male wasps.[15] In a study done by Strassmann, it was found that sexual investment is female biased, especially during years of high predation and when nests are generally less successful.[15] It has been seen that in Polistes exclamans, females are produced before males.[15] This female investment bias may result from the ability of females to become either a worker or a reproductive (this is more adaptive for the nest as it gives the nest increased flexibility compared to that of males and may be important for overall nest success) or because females provide the colony with greater nest defense.[15] Furthermore, even when the original queen is no longer present, the sexual investment is still biased towards females, which is not expected given the genetic implications of a new queen.[15]

Workers first emerge between May and July. During this first emergence, reproductive males also emerge with this first brood and are called the early males.[14] These males provide a great adaptation because they allow for the presence of reproductive males. Nests with a greater number of early males produce a greater number of workers, cells, pupae, and emergences.[14] Although it is uncertain as to why, queen deaths are common in Polistes exclamans, most frequently in May. The majority of original queens are dead by July, “well before eggs that became autumn reproductive were laid,” as reproductives emerged from late August through September.[14] Thus, queens must remain alive post June in order to birth future reproductives, and if they are not able to do so, the eldest former worker then becomes the new queen.[18] The reproductive early males produced may be adaptive for the deaths of the queens so that the colony will not die and will be able to continue.[14]

As with many other insects, P. exclamans use sexual pheromones to attract members of the opposite sex. Researchers have attempted to determine the exact role that sexual pheromones play in sexual attraction in paper wasps. They set up a wind tunnel where males and females were exposed to a sexual pheromone wick that was isolated from males and females.[22] These pheromones were taken by hexane extracts from wholes bodies and thoraces of unmated females. The male extracts were taken from the ectal mandibular and seventh sternal glands. It was found that the opposite sex was attracted upwind of the scent and the results were intensified when a fan was turned on.[22] The range of the scent was found to be around 2 metres.[22]

In nature, it was observed that males would venture away from the nest in order to attract females. The males would press their gastral sterna against a perch and rub their mandibular against it. This is used to attract females, and females were observed to visit these branches and sample the scents.[22] It was also found that on some occasions, males were attracted to other male scents.[22]

Polistes exclamans have comparably smaller nests and colonies compared to certain other eusocial wasps species. Thus, it is common for the nest to be destroyed, for the queen to die prior to the end of the season, or for the nest to fail through other means, such as predation, parasitisation, or worker mortality, leading workers to help rear broods that are less related to them than are their sister wasps.[13] Due to this, it would be advantageous for there to be wasps that remain casteless till adulthood, allowing female wasps’ reproductive capabilities to be plastic.[13] It has thus been predicted that Polistes exclamans worker wasps would act as workers if a brood were present and would begin to develop gyne characteristics if a brood was not present, exhibiting the aforementioned adaptive plasticity.[13] Experimentally, this has been found to be true. Solis and Strassmann conducted a study in which an experimental group had the eggs and larvae removed. In the experimental group, the wasps began to behave like future queens and even developed the characteristic layer of fat common to queens. In the control group, the wasps continued to behave as workers. This is indicative that the presence of the brood affects caste differentiation and that Polistes exclamans female workers exhibits adaptive reproductive plasticity.[13]

Nest mate recognition is widespread in many eusocial insects. However, when there are no physical differences in either diet or nesting materials, many can no longer discriminate nest mate from other conspecifics.[23] Recently, however, it has been indicated that many insects have the ability to discriminate relatives even having lived in identical environments with their non-relative counterpart.[23] Studies have indicated that in Polistes fuscatus, former nestmates would nest in areas located close together near their natal nests the following spring. Social halictid bees also are able to recognize their relatives.[23] In Polistes exclamans, it has been indicated that discrimination of relatives from non-relatives, even having lived in identical environments, is possible. This was found by Allen, Schulze Kellman, and Gamboa through an experiment in which hibernating wasps from different nests were put in a box together after being raised in identical environments.[23] As the number of unrelated groups of wasps increased, the more clumps were formed in the box. This indicates that the wasps were capable of differentiating relative from nonrelative, an adaptive ability in the defense of their nest.[23]

Many Polistes species aggregate over winter. Polistes exclamans clump in rather large aggregations while hibernating.[24] “These aggregations are frequently found in protected places called hibernacula that can be as varied as crevices and cracks of rocks or trunks, beneath the bark of trees, between walls of buildings, or any other natural or artificial structures that provides protection during hibernation.”[24] Although Polistes exclamans have been found in organ-pipe muddauber nests, a mud nest that can provide a certain level of protection, it is not thought to actually house hibernating Polistes exclamans over the winter.[24]

A typical P. exclamans worker will live somewhere between 14–16 days. The oldest wasp to be observed in a natural colony was 102 days old. Queens typically live 6 times longer than the workers.[5] There has been some variation observed with the life expectancy of P. exclamans. This has typically been attributed to the colony of origin. It also must be noted that while there is variation between colonies, there typically is less variation between the original nest and the satellite nest.[5] Variation inside a single colony may also occur because of the presence of different castes.[25] Foragers, those who frequently travel outside the nest, lead a riskier life and tend to have lower life expectancy than those who stay in the nest. Though, it was observed that colonies with high foraging rates also had higher reproductive rates to compensate for the lower life expectancy.[5] Another aspect that was observed in one nest was that an increase in female population led to lower longevity. This was because the females were more focused on competing to become the next queen than foraging and fulfilling their roles.[5]

Worker death is an important factor in colony failure, and is the cause of 13–76% of colony failures. This may be in part due to the small colony size. Because the colonies are small, worker longevity is crucial for the survival of the nest.[5]

Throughout the 1950s and 1960s, Polistes exclamans was extending its range, spreading into the midwestern United States.[3] Between 1958 and 1967 the following states had new state records of Polistes exclamans: Illinois, Indiana, Kentucky, Maryland, Missouri, New Jersey, New Mexico, Tennessee, and Virginia.[3] The behavioral basis behind range expansion could be based upon the exceptional capability Polistes exclamans has in the colonization of new sites and the solitary nest founding behavior Polistes exclamans exhibits.[3] This capability could be assisted by the “associated tendencies of P. exclamans foundresses to disperse and to move into newly available nesting sites.”[3] Meanwhile, species with social foundresses reproduce near the place of origin and, therefore, do not extend their range as far.[3]

Nest in Molokai, Hawaii

Satellite nests are common among P. exclamans. A queen will fly somewhere between 0.15 and 11m away from the original nest to settle in a new location. About 16–39% of nests create satellites from May to July.[14]

Similar to the wasp species Parischnogaster alternata, which constructs multitude of nests in clusters in order to create protection through dilution effect, the satellites are used as an insurance against attacks by predators and parasitoids.[26] Of 12 nests that were knocked down by birds that had satellites, 66.7% of the colony survived by moving to the satellite, compared to the reproductive success of nests with no satellite that were attacked (5.7%).[14] When Chalcoela iphitalis invades, the prevalence of satellites didn't increase the survivability, however it did have an effect when Elasmus polistis were introduced. It gave the P. exclamans a place to escape to.[14]

Workers who are older typically start satellite nests and have more developed ovaries. Younger and lesser-developed workers join the satellite after its establishment. The distribution of workers between the main nest and satellite is very important because they are needed for the establishment of the satellite and for the continued running of the main nest. Although, after several months the main nest can become abandoned.[14] If not enough workers follow the initial worker to the new nest, then it will most likely fail as a satellite.[14]

Polistes exclamans live in social nests that are open combed.[27] This leaves nests very susceptible to attacks by predators and by parasitoids. Attack by parasitoids will occur in the nest brood, as the invader will attempt to insert its own offspring into the host's nest. The two most common parasitoids are Chalcoela iphitalis and Elasmus polistis.

P. exclamans take several countermeasures against invasions. If the wasps detect an intruder they will violently bite and sting the location where the C. iphitalis moth has passed by.[27] This will cause vibrations inside of the nest and the wasps inside will become alarmed and will move around jerkily. This phenomenon is known as the parasite dance. This will continue for up to 10 hours after the moth has been detected. If the moth is found it will be eaten immediately. However, this is not likely to happen. The moth lays its eggs in the nest, and when they hatch, they will take over the nest. As a result, many wasp pupae are aborted. This is commonly seen in the late summer when the moth is most abundant.

The second common parasitoid, Elasmus polistis, also has disastrous effects on the P. exclamans nest. Up to 80 E. polistis will hatch out of a single cell. The males emerge first, and then will exit the nest and wait until the females emerge. Once the female moths emerge, the males will mate and reproduce, quickly destroying the P. exclamans population.[28] In some cases the invading E. polistis will hide larvae in the nest so that the P. exclamans cannot find them. This is important to the survival of P. exclamans. The hosts will attempt to remove as many parasitoids as possible to prevent them from spreading to nearby nests. This defense mechanism has not been proven to be effective, as more often than not the E. polistis larvae go undetected by the hosts. In one experiment conducted, it was found that over 60% of all nests lost brood to the parasitoids E. polistis or C. iphitalis in 1981.[28] It was also observed that larger satellites have a greater risk of being parasitized. This is due to the fact that the large nests are typically older.[29] This relationship between age and parasitism can be seen with the fact that older queens’ nests tend to be parasitized more than younger queens’ nests.[5]

An overpopulated Polistes exclamans nest

P. exclamans has several different predators. Their most dangerous predators are birds, which will fly by and knock the nest to the ground. This phenomenon was discovered by the disappearance of nests, and wasp nests being found on the ground near the bird's nest. It has also been found that birds eat the larvae from the P. exclamans nest. This attack kills the whole nest but it isn't very effective against the adults from the colony. When attacked, the adults fly away either to another colony or a satellite colony.

Attack by the ant Crematogaster laeviuscula is different from attacks by birds. Rather than knocking down the nest, the ants swarm all over the nest and remove the brood from it. The ants destroy the entire brood but they are unable to kill the adults.[27]

Other predators attack the adults while they are foraging or traveling to satellite nests. It is difficult to determine the number of adults that are killed away from the nest because they are difficult to track. However, it has been observed that the queen's flight to the satellite nest isn't dangerous and does not reduce its survivability.[2]

The idea of group living has been discussed by many evolutionists for its costs and benefits. Alexander predicted that the cost to large groups is that they are more likely to suffer from parasitism, but at the same time, they benefit by defense against predation. This theory is not supported in the case of P. exclamans because the parasitism does not always increase with nest size and the predation rate was independent of size.[30]

Some animals may assess rivals through specific characteristics that are relevant to their fighting abilities; however, some animals, instead, use conventional characteristics to determine the competitive ability of their rivals.[31] Conventional characteristics are signals that are indicative of the rival's abilities but are not a reflection of the animal's physiological or physical agonistic abilities.[31] Polistes exclamans uses facial patterns as a conventional characteristic to determine the agonistic abilities of competitive rivals. Larger wasps have a greater amount of brown pigmented clypei. Wasps use these pigmented clypei to determine the viability of challenging a rival.[31] The more pigmented the clypei or the larger the Polistes exclamans, the more likely the Polistes exclamans will be willing to challenge a rival. The less pigmented the clypei or the smaller the Polistes exclamans, the less willing it would be to challenge a rival.[31] This assists in the minimization of the “costs of conflict during dominance competition among nest-founding queens.”[31]

Polistes exclamans exhibit an alarm response, typical of many higher level eusocial Hymenoptera.[32] Although it has been previously indicated that nest mates may alarm the rest of the nest through jerky movement and buzzing of wings, Polistes exclamans is capable of alarming the rest of the nest and attracting attacking wasps through chemical means by releasing a non-species-specific venomous alarm pheromone.[32] Although a chemical signal to warn against attack would be evolutionarily adaptive, the Polistes exclamans only releases a response post the initial attack from and on the predator, as the venom is only released during the sting.[32] This venom is capable of warning and coordinating a response from the nest and attracting nearby heterospecific or conspecific females from nearby colonies to attack the predator.[32] This helps deter the predator from further attacking and is especially adaptive for the defense of the nest. Although certain other insects have been able to adapt the release of the pheromone to other means of communicating alarm, Polistes exclamans has yet to do so, indicating that it is still in its “primitive state in evolution of the complex systems of communication of alarm seen in higher social insects.”[32] In one experiment, pheromones were extracted from female glands and sacs and were spread onto venom paper. It was then found that females were attracted upwind of the venom paper and some even attempted to sting it. This also had a heterospecific response where others also reacted to it. While it was found that one wasp couldn't release enough pheromones to elicit a response, if many individuals were alarmed they would elicit a response from the rest of the nest. However, this scent is not strong enough to reach other nests, and so neighboring nests must be alarmed by physical movements and wing flapping.[32]

After being invaded, a queen may be forced to leave her nest. If a satellite has not been established, the queen may usurp another queen in a different nest. Thus not having to invest any valuable resources, the queen can then use these resources on reproducing a new brood.[33]