Streptomyces coelicolor

Streptomyces coelicolor is a soil-dwelling Gram-positive bacterium that belongs to the genus Streptomyces.

Streptomyces coelicolor
Scientific classification
Domain:
Bacteria
Phylum:
Actinobacteria
Order:
Actinomycetales
Family:
Streptomycetaceae
Genus:
Streptomyces
Species:
S. coelicolor
Binomial name
Streptomyces coelicolor
(Müller, 1908) Waksman and Henrici, 1948

Genome

The genome of one strain of S. coelicolor was sequenced in 2002.[1] It contains 8,667,507 bp, encoding 7,825 predicted genes, including over 20 gene clusters for the synthesis of known or predicted natural products.[2]

Small noncoding RNA
See also: s-SodF RNA

Bacterial small RNAs are involved in post-transcriptional regulation. Using deep sequencing S. coelicolor transcriptome was analysed at the end of exponential growth. 63 small RNAs were identified. Expression of 11 of them was confirmed by Northern blot. The sRNAs were shown to be only present in Streptomyces species.[3]

sRNA scr4677 (Streptomyces coelicolor sRNA 4677) is located in the intergenic region between anti-sigma factor SCO4677 gene and a putative regulatory protein gene SCO4676. scr4677 expression requires the SCO4677 activity and scr4677 sRNA itself seem to affect the levels of the SCO4676-associated transcripts.[4]

Targets of two of S. coelicolor noncoding RNAs have been identified. Noncoding RNA of Glutamine Synthetase I was shown to modulate antibiotic production.[5] The small RNA scr5239 (Streptomyces coelicolor sRNA upstream of SCO5239) has two targets. It inhibits agarase DagA expression by direct base pairing to the dagA coding region, and it represses translation of methionine synthase metE (SCO0985) at the 5' end of its open reading frame.[6][7]

Usage in biotechnology

Strains of S. coelicolor produce various antibiotics, including actinorhodin, methylenomycin, undecylprodigiosin,[8] and perimycin.[9][10] Certain strains of S. coelicolor can be used for heterologous protein expression.[2]

References
  1. Bentley SD, Chater KF, Cerdeño-Tárraga AM, et al. (May 2002). "Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)". Nature. 417 (6885): 141–7. Bibcode:2002Natur.417..141B. doi:10.1038/417141a. PMID 12000953. S2CID 4430218.
  2. "Streptomyces coelicolor". John Innes Center. Archived from the original on 19 October 2005. Retrieved 25 January 2010.
  3. Vockenhuber, Michael-Paul; Sharma, Cynthia M.; Statt, Michaela G.; Schmidt, Denis; Xu, Zhenjiang; Dietrich, Sascha; Liesegang, Heiko; Mathews, David H.; Suess, Beatrix (May 2011). "Deep sequencing-based identification of small non-coding RNAs in Streptomyces coelicolor". RNA Biology. 8 (3): 468–477. doi:10.4161/rna.8.3.14421. ISSN 1555-8584. PMC 3218513. PMID 21521948.
  4. Hindra, null; Moody, Matthew J.; Jones, Stephanie E.; Elliot, Marie A. (2014-01-01). "Complex intra-operonic dynamics mediated by a small RNA in Streptomyces coelicolor". PLOS ONE. 9 (1): e85856. Bibcode:2014PLoSO...985856H. doi:10.1371/journal.pone.0085856. ISSN 1932-6203. PMC 3896431. PMID 24465751.
  5. D'Alia, Davide; Nieselt, Kay; Steigele, Stephan; Müller, Jonas; Verburg, Ilse; Takano, Eriko (2010-02-01). "Noncoding RNA of glutamine synthetase I modulates antibiotic production in Streptomyces coelicolor A3(2)". Journal of Bacteriology. 192 (4): 1160–1164. doi:10.1128/JB.01374-09. ISSN 1098-5530. PMC 2812974. PMID 19966003.
  6. Vockenhuber, Michael-Paul; Suess, Beatrix (2012-02-01). "Streptomyces coelicolor sRNA scr5239 inhibits agarase expression by direct base pairing to the dagA coding region". Microbiology. 158 (Pt 2): 424–435. doi:10.1099/mic.0.054205-0. ISSN 1465-2080. PMID 22075028.
  7. Vockenhuber, Michael-Paul; Heueis, Nona; Suess, Beatrix (2015-01-01). "Identification of metE as a second target of the sRNA scr5239 in Streptomyces coelicolor". PLOS ONE. 10 (3): e0120147. Bibcode:2015PLoSO..1020147V. doi:10.1371/journal.pone.0120147. ISSN 1932-6203. PMC 4365011. PMID 25785836.
  8. Brian P, Riggle PJ, Santos RA, Champness WC (June 1996). "Global negative regulation of Streptomyces coelicolor antibiotic synthesis mediated by an absA-encoded putative signal transduction system". J. Bacteriol. 178 (11): 3221–31. doi:10.1128/jb.178.11.3221-3231.1996. PMC 178074. PMID 8655502.
  9. Liu CM, McDaniel LE, Schaffner CP (March 1972). "Fungimycin, biogenesis of its aromatic moiety". The Journal of Antibiotics. 25 (3): 187–8. doi:10.7164/antibiotics.25.187. PMID 5034814.
  10. Lee CH, Schaffner CP (May 1969). "Perimycin. The structure of some degradation products". Tetrahedron. 25 (10): 2229–32. doi:10.1016/S0040-4020(01)82770-8. PMID 5788396.

Further reading
  • Galet, Justine; Deveau, Aurélie; Hôtel, Laurence; Leblond, Pierre; Frey‐Klett, Pascale; Aigle, Bertrand (September 2014). "Gluconic acid‐producing Pseudomonas sp. prevent γ‐actinorhodin biosynthesis by Streptomyces coelicolor A3(2)". Archives of Microbiology. 196 (9): 619–627. doi:10.1007/s00203-014-1000-4. PMID 24906569. S2CID 9759014.


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