CCDC82

CCDC82
Identifiers
Aliases	CCDC82, HSPC048, coiled-coil domain containing 82
External IDs	MGI: 1913646 HomoloGene: 11678 GeneCards: CCDC82
Gene location (Human)
Chr.	Chromosome 11 (human)[1]
End	96,389,956 bp[1]
Gene location (Mouse)
Chr.	Chromosome 9 (mouse)[2]
End	13,292,520 bp[2]
Orthologs
	79780
	66396
	ENSG00000149231
	ENSMUSG00000079084
	Q8N4S0
	Q6PG04
	NM_014148; NM_024725; NM_001318736; NM_001318737; NM_001363594
	NM_025534
	NP_001305665; NP_001305666; NP_079001; NP_001350523
	NP_079810
	Wikidata
View/Edit Human	View/Edit Mouse

Coiled-Coil Domain Containing protein 82 (CCDC82) is a protein that in humans, is encoded for by the gene of the same name, CCDC82. The CCDC82 gene is expressed in nearly all of human tissues at somewhat low rates. As of today, there are no patents involving CCDC82 and the function remains unknown.

Gene

CCDC82 is located on chromosome 11 at 11q21.5.[5] It contains two domains of unknown function, DUF4196 and DUF4211.[6] The DNA sequence is 37,155 base pairs long[7] and contains 7 exons.[8]

Homology

CCDC82 is present in many orthologs. It is conserved throughout other mammals, reptiles, birds and bony fish. It is not found in invertebrates, bacteria or fungi. There are no paralogs.[9]

Unrooted phylogenetic tree

Scientific name	Common name	Date of divergence	Accession number	Length	Percent identity	Percent similarity
Pan troglodytes	Chimpanzee	6.3 Mya	XP_001147806.1	544 aa	60.8	99
Gorilla gorilla gorilla	Gorilla	8.8 Mya	XP_004052053.1	521 aa	56.2	91
Pongo abelii	Orangutan	15.7 Mya	NP_003253075.1	343 aa	95.3	98
Nomascus leucogenys	Crested gibbon	20.4 Mya	XP_003253075.1	554 aa	57.8	94
Papio anubis	Olive baboon	29 Mya	XP_003910631.1	542 aa	52	86
Callithrix jacchus	Marmoset	42.6 Mya	XP_002754732.1	526 aa	51.2	86
Mus musculus	Mouse	92.3 Mya	NP_079810.2	518 aa	39.7	74
Rattus norvegicus	Brown rat	92.3 Mya	NP_001007661.1	516 aa	37.4	71
Canis lupus familiaris	Dog	94.2 Mya	XP_542232.2	520 aa	46	79
Bos taurus	Cow	94.2 Mya	NP_001039559.2	522 aa	42.4	74
Ailuropoda melanoleuca	Giant panda	94.2 Mya	XP_002925755.1	528 aa	45.4	80
Loxodonta africana	African bush elephant	98.7 Mya	XP_003415705.1	521 aa	40.4	75
Sarcophilus harrisii	Tasmanian devil	162.2 Mya	XP_003764344.1	518 aa	36.9	70
Monodelphis domestica	Gray short-tailed opossum	162.2 Mya	XP_001363143.1	516 aa	36.7	72
Ornithorhynchus anatinus	Platypus	167.4 Mya	XP_001511067.1	505 aa	26.6	70
Gallus gallus	Chicken	296 Mya	XP_423807.3	460 aa	24.3	56
Meleagris gallopavo	Wild turkey	296 Mya	XP_003203546.1	462 aa	21.5	70
Taeniopygia guttata	Zebra finch	296 Mya	XP_002198267.1	575 aa	20	61
Anolis carolinensis	Carolina anole	296 Mya	XP_003219357.1	603 aa	19.9	49
Xenopus tropicalis	Western clawed frog	371.2 Mya	XP_002935613.1	462 aa	21	73

mRNA

Promoter

The predicted promoter for CCDC82 is located on the minus strand and spans from base pairs 96,122,963 to 96,123,587. It is 625 base pairs long.[10]

Transcription factors

The transcription factors listed below are for the predicted promoter sequence and are located on the minus strand.[11]

Detailed Family Information	Span	Score
Alternative splicing variant of FOXP1	48-64	1.00
Homeodomain transcription factor Otx2	34-50	.992
Hypoxia-response Elements	111-127	.985
Homeobox A10/HOX 1.8	52-68	.957
SRY box 9	80-104	.947
Mesoderm posterior 1 and 2	42-62	.937
c-Myc/Max heterodimer	44-60	.929
SAM pointed domain containing ets transcription factor	27-47	.923
cAMP-responsive element binding protein	134-154	.917
PR domain zinc finger protein 14	138-152	.912

Protein

The protein it encodes for is 344 amino acids in length. The protein itself is very acidic and is very rich in aspartic acid and glutamic acid. It is also very deficient in alanine, containing only two alanines in the entire sequence. The alanines are located adjacent to each other, amino acid number 233 and 234. Alanine 233 is highly conserved throughout the orthologs. The molecular weight is 40.0 kdal and the isoelectric point is 4.383[12]

Expression

This image shows the level of expression of CCDC82 in different tissues throughout the body.

CCDC82 is found in nearly all tissues in the human body, however it is present in higher quantities in the skeletal muscles, adrenal cortex, and the trigeminal ganglion.[13][14]

Post translational modifications

CCDC82 has several predicted phosphorylation sites.[15] There are 32 predicted serine phosphorylation sites, 5 threonine, and 3 tyrosine.[16]

Interactions

CCDC82 is known to interact with two proteins. It indirectly interacts with VHL, a gene that encodes for a tumor suppressor and ubiquitin protein ligase. It also interacts with EWSR1, which functions as a transcriptional repressor.[17]

Clinical significance

CCDC82 is a circulat-responsive gene.[18] Circulat is a product designed to restore systemic vascular health. It is a plant based product and taken by patients who suffer from diabetes or circulatory problems.[19]

Possible function

Based on the information that CCDC82 is affected by the Circulat product it could be hypothesized that CCDC82 is involved in circulatory function. However, this is purely speculation.

References

GRCh38: Ensembl release 89: ENSG00000149231 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000079084 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"CCDC82 Genomic Views". GeneCards. Weizmann Institute of Science.
"CCDC82 Protein Domains and Families". GeneCards. Weizmann Institute of Science.
"Homo sapiens chromosome 11, GRCh37.p10 Primary Assembly". NCBI.
"Prediction of several variants of multiple genes". Softberry.
"CCDC82 BLAST". BLAST. NCBI.
"Genome Browser". ElDorado. Genomatix.
"Transcription Factor Binding Sites". ElDorado. Genomatix.
"SAPS". Biology Workbench. SDSU.
"Large-scale analysis of the human transcriptome (HG-U133A)". GeoProfiles. NCBI.
Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL (August 2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–8. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
"CCDC82". NetPhos 2.0. Center for Biological Analysis.
"CCDC82". Gene Cards. Weizmann Institute of Science. Retrieved 2 May 2013.
Antoshechkin A, Olalde J, Magarici M, Muhammad A, Salom A, Suarez J, Amendola F (August 2007). "Analysis of effects of the herbal preparation Circulat on gene expression levels in cultured human fibroblasts". Phytother Res. 21 (8): 777–89. doi:10.1002/ptr.2174. PMID 17514633. S2CID 29862628.
"About Circulat". Circulat. Circulat Biotech LLC. Archived from the original on 2013-08-31.

External links

Human CCDC82 genome location and CCDC82 gene details page in the UCSC Genome Browser.

This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000149231 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000079084 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[5] "CCDC82 Genomic Views". GeneCards. Weizmann Institute of Science.

[6] "CCDC82 Protein Domains and Families". GeneCards. Weizmann Institute of Science.

[7] "Homo sapiens chromosome 11, GRCh37.p10 Primary Assembly". NCBI.

[FGENES-M-8] "Prediction of several variants of multiple genes". Softberry.

[9] "CCDC82 BLAST". BLAST. NCBI.

[10] "Genome Browser". ElDorado. Genomatix.

[11] "Transcription Factor Binding Sites". ElDorado. Genomatix.

[12] "SAPS". Biology Workbench. SDSU.

[13] "Large-scale analysis of the human transcriptome (HG-U133A)". GeoProfiles. NCBI.

[pmid10931946-14] Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL (August 2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–8. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.

[pmid17081983-15] Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (November 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.

[16] "CCDC82". NetPhos 2.0. Center for Biological Analysis.

[17] "CCDC82". Gene Cards. Weizmann Institute of Science. Retrieved 2 May 2013.

[pmid17514633-18] Antoshechkin A, Olalde J, Magarici M, Muhammad A, Salom A, Suarez J, Amendola F (August 2007). "Analysis of effects of the herbal preparation Circulat on gene expression levels in cultured human fibroblasts". Phytother Res. 21 (8): 777–89. doi:10.1002/ptr.2174. PMID 17514633. S2CID 29862628.

[19] "About Circulat". Circulat. Circulat Biotech LLC. Archived from the original on 2013-08-31.