Centromere protein E

CENPE
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1T5C
Identifiers
Aliases	CENPE, CENP-E, KIF10, PPP1R61, MCPH13, Centromere protein E
External IDs	OMIM: 117143 MGI: 1098230 HomoloGene: 20429 GeneCards: CENPE
Gene location (Human)
Chr.	Chromosome 4 (human)[1]
End	103,198,445 bp[1]
Gene location (Mouse)
Chr.	Chromosome 3 (mouse)[2]
End	135,273,611 bp[2]
RNA expression pattern
	More reference expression data
Gene ontology
Molecular function	• nucleotide binding; • ATPase activity; • ATP binding; • GO:0001948 protein binding; • microtubule motor activity; • microtubule binding; • kinetochore binding;
Cellular component	• spindle; • kinesin complex; • chromosome; • cell nucleus; • condensed chromosome kinetochore; • condensed chromosome outer kinetochore; • chromosome, centromeric region; • membrane; • midbody; • microtubules; • kinetochore; • condensed chromosome, centromeric region; • microtubule cytoskeleton; • mitotic spindle midzone; • cytoskeleton; • cytoplasm; • cytosol; • kinetochore microtubule; • nucleoplasm; • spindle midzone;
Biological process	• regulation of mitotic metaphase/anaphase transition; • cell cycle; • positive regulation of protein kinase activity; • multicellular organism development; • cell division; • antigen processing and presentation of exogenous peptide antigen via MHC class II; • kinetochore assembly; • microtubule-based movement; • sister chromatid cohesion; • retrograde vesicle-mediated transport, Golgi to ER; • metaphase plate congression; • lateral attachment of mitotic spindle microtubules to kinetochore; • GO:0007067 mitotic cell cycle; • GO:0043148 mitotic spindle organization; • mitotic chromosome movement towards spindle pole; • microtubule plus-end directed mitotic chromosome migration; • chromosome segregation; • mitotic metaphase plate congression; • attachment of mitotic spindle microtubules to kinetochore;
	Sources:Amigo / QuickGO
Orthologs
	1062
	229841
	ENSG00000138778
	ENSMUSG00000045328
	Q02224
	Q6RT24
	NM_001286734; NM_001813
	NM_173762
	NP_001273663; NP_001804
	NP_776123
	Wikidata
View/Edit Human	View/Edit Mouse

Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene.[5][6]

Centromere-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.[6]

Clinical significance

Mutations in CENPE result in autosomal recessive primary microcephaly type 13, which includes skeletal abnormalities and immunodeficiency.[7]

References

GRCh38: Ensembl release 89: ENSG00000138778 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000045328 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Testa JR, Zhou JY, Bell DW, Yen TJ (Mar 1995). "Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24→q25 and 1q32→q41, respectively, by fluorescence in situ hybridization". Genomics. 23 (3): 691–3. doi:10.1006/geno.1994.1558. PMID 7851898.
"Entrez Gene: CENPE centromere protein E, 312kDa".
"OMIM Entry - # 616051 - MICROCEPHALY 13, PRIMARY, AUTOSOMAL RECESSIVE; MCPH13". www.omim.org. Retrieved 2020-01-25.

Yen TJ, Li G, Schaar BT, et al. (1992). "CENP-E is a putative kinetochore motor that accumulates just before mitosis". Nature. 359 (6395): 536–9. doi:10.1038/359536a0. PMID 1406971. S2CID 4306312.
Yen TJ, Compton DA, Wise D, et al. (1991). "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase". EMBO J. 10 (5): 1245–54. doi:10.1002/j.1460-2075.1991.tb08066.x. PMC 452779. PMID 2022189.
Thrower DA, Jordan MA, Schaar BT, et al. (1995). "Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor". EMBO J. 14 (5): 918–26. doi:10.1002/j.1460-2075.1995.tb07073.x. PMC 398164. PMID 7889940.
Wood KW, Sakowicz R, Goldstein LS, Cleveland DW (1997). "CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment". Cell. 91 (3): 357–66. doi:10.1016/S0092-8674(00)80419-5. PMID 9363944. S2CID 12216144.
Cooke CA, Schaar B, Yen TJ, Earnshaw WC (1998). "Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase". Chromosoma. 106 (7): 446–55. doi:10.1007/s004120050266. PMID 9391217. S2CID 18884489.
Zecevic M, Catling AD, Eblen ST, et al. (1998). "Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E". J. Cell Biol. 142 (6): 1547–58. doi:10.1083/jcb.142.6.1547. PMC 2141767. PMID 9744883.
Chan GK, Schaar BT, Yen TJ (1998). "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1". J. Cell Biol. 143 (1): 49–63. doi:10.1083/jcb.143.1.49. PMC 2132809. PMID 9763420.
Chan GK, Jablonski SA, Sudakin V, et al. (1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". J. Cell Biol. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750.
Ashar HR, James L, Gray K, et al. (2000). "Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules". J. Biol. Chem. 275 (39): 30451–7. doi:10.1074/jbc.M003469200. PMID 10852915.
Uren AG, Wong L, Pakusch M, et al. (2001). "Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype". Curr. Biol. 10 (21): 1319–28. doi:10.1016/S0960-9822(00)00769-7. PMID 11084331. S2CID 18455745.
Suzuki Y, Tsunoda T, Sese J, et al. (2001). "Identification and characterization of the potential promoter regions of 1031 kinds of human genes". Genome Res. 11 (5): 677–84. doi:10.1101/gr.gr-1640r. PMC 311086. PMID 11337467.
Liu ST, Chan GK, Hittle JC, et al. (2003). "Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores". Mol. Biol. Cell. 14 (4): 1638–51. doi:10.1091/mbc.02-05-0074. PMC 153128. PMID 12686615.
Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197. S2CID 27764390.
Garcia-Saez I, Blot D, Kahn R, Kozielski F (2005). "Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure". Acta Crystallogr. D. 60 (Pt 6): 1158–60. doi:10.1107/S0907444904009564. PMID 15159587.
Tanudji M, Shoemaker J, L'Italien L, et al. (2005). "Gene silencing of CENP-E by small interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay". Mol. Biol. Cell. 15 (8): 3771–81. doi:10.1091/mbc.E03-07-0482. PMC 491836. PMID 15181147.
Garcia-Saez I, Yen T, Wade RH, Kozielski F (2004). "Crystal structure of the motor domain of the human kinetochore protein CENP-E". J. Mol. Biol. 340 (5): 1107–16. doi:10.1016/j.jmb.2004.05.053. PMID 15236970.
Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. (2005). "Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation". EMBO J. 23 (16): 3237–48. doi:10.1038/sj.emboj.7600347. PMC 514520. PMID 15297875.
Mao Y, Desai A, Cleveland DW (2005). "Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling". J. Cell Biol. 170 (6): 873–80. doi:10.1083/jcb.200505040. PMC 2171436. PMID 16144904.
Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.

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[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000138778 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000045328 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7851898-5] Testa JR, Zhou JY, Bell DW, Yen TJ (Mar 1995). "Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24→q25 and 1q32→q41, respectively, by fluorescence in situ hybridization". Genomics. 23 (3): 691–3. doi:10.1006/geno.1994.1558. PMID 7851898.

[entrez-6] "Entrez Gene: CENPE centromere protein E, 312kDa".

[7] "OMIM Entry - # 616051 - MICROCEPHALY 13, PRIMARY, AUTOSOMAL RECESSIVE; MCPH13". www.omim.org. Retrieved 2020-01-25.

Centromere protein E

Clinical significance

See also

References

Further reading