Dai Gyakuten Saiban 2: Naruhodō Ryūnosuke no Kakugo

Two months after the events of the previous game, Susato Mikatoba learns that her best friend, Haori Murasame, has been accused of murdering Jezail Brett, the murderer of Dr. Watson. With no defense attorney willing to help, she decides to do so. However, since women aren't allowed in Japanese courts, she disguises herself as Ryūnosuke Naruhodō's cousin Ryutaro. Aided by her father, who never was sick, she discovers that Brett's true killer is Heita Mamemomi, a reporter who hated that she was to be judged in China instead of Japan. Unfortunately, with Brett's death, the reason for her murder of John Watson remains a mystery. Susato learns that the reason she was summoned back to Japan is connected to a case she previously worked on.

In England, Ryūnosuke receives a letter from Susato asking him to look into the case in question. Since he has been banned from the courtroom for revealing government secrets, he has nothing better to do, so he does so. One day after being cleared, Soseki Natsume is accused again, this time of poisoning William Petenshy, a failed actor and fellow boarder in a building Natsume claims is cursed by the malevolent spirit of Selden, a criminal who lived in the same flat Natsume now does. Though Petenshy survives, he sues Natsume for trying to kill him. Ryūnosuke and Susato once again go up against infamous prosecutor Barok van Zieks, who has the reputation of all the people he tries dying no matter what. Ryūnosuke eventually learns that Petenshy was poisoned by Viridian Green, the victim in Natsume's first case. Petenshy had been a cellmate of Selden and heard of the treasure he'd hid in his flat. When he'd failed to rent the flat, he blew on the pipe of the building's shared gas main which filled the flat with lethal gas, killing the previous boarder, Green's fiance Duncan Ross, in the hopes of getting in, then tried the same with Natsume. When she figured it out, she covered the pipe with strychnine. After the case, Natsume goes back to Japan, while Ryūnosuke finds the treasure, a diamond studded dog collar which Sherlock Holmes forbids mention of.

Sometime later, an explosion during a demonstration of a teleportation device at the World's Fair results in the death of crime lord Elyder Meningen. The machine's inventor, Benjamin Dobinbough, who is a friend of van Zieks, is arrested. Ryūnosuke gets permission from Chief Magistrate Hart Vortex to return to court and faces van Zieks and his masked apprentice (who Vortex assigned to him) with the help of a returned Susato. During the case, they find a connection to the Professor, a serial killer who murdered people through his dog, one victim being van Zieks' brother Klimt, the collar belonging to the dog. They also learn that the device was a fraud funded by Meningen to get a huge grant. They eventually prove the killer to be Scotland Yard's Chief Coroner Courtney Sithe, who Meningen was blackmailing over her falsely declaring the Professor dead since he seemingly "rose from the dead" afterwards. After the machine's designer, Enoch Drebber, whose life Meningen had ruined, set it up to kill him and used a stolen wax figure of the Professor to fake the body's location, she finished the job when he survived. Afterwards, van Zieks sends Dobinbough away to avoid the "Grim Reaper" curse. At the same time, the wax sculpture is unmasked and the apprentice, in shock, removes his own mask to reveal a still alive Kazuma Asōgi, whose memory was lost when he was hurt and then restored by the statue's face, and who states that the face belongs to his father Genshin.

Detective Tobias Gregson is murdered and van Zieks is arrested as the prime suspect. Despite their rivalry, Ryūnosuke takes his defense against Asōgi, who wishes to avenge his father who was sent to his death by van Zieks, in a trial presided over by Vortex himself. It eventually comes out that Vortex is the head of an international group of assassins, the true Grim Reaper, whose members, past and present, include Brett, Genshin, Kazuma, Klimt, and Japanese judge Seshiro Jigoku, with Watson, Gregson and Sithe aiding, and whose ultimate goal is a crime free England. It started with the Professor killings, which Klimt actually committed. When Vortex found out, he blackmailed him into targeting people he chose. When Genshin figured out Klimt's identity, Vortex wouldn't do anything, so he killed Klimt in a duel. When Vortex figured it out, he framed Genshin for the other murders, only for Genshin to use Klimt's will that would've exposed him to facilitate a prison escape. When the attempt was nearly exposed, Vortex had Jigoku kill Genshin. Both Watson and Gregson were killed to ensure Vortex's crimes were never learned. Vortex sent Asōgi to kill Gregson and when he was injured, Jigoku took it upon himself to do it. Though Naruhodo finds the will, Vortex is confident he's untouchable, only to learn that Holmes has alerted the queen, who strips him of his rank and leaves him to be arrested. It's also revealed that Iris Watson's father isn't Watson but Klimt. Before his death, Genshin sent Susato's father to find Klimt's wife, who died in childbirth. He then left the infant Iris with his friend Holmes, who, in keeping with both Asogi and Mikatoba's wishes, made sure Iris never published the manuscript based on the Professor case. Ryūnosuke makes peace with both van Zieks and Asōgi, who decides to remain a prosecutor, and returns to Japan with Susato to continue defending the innocent.

• Official website
• Dai Gyakuten Saiban 2: Naruhodō Ryūnosuke no Kakugo at The Visual Novel Database