Drosophila

Drosophila species are found all around the world, with more species in the tropical regions. Drosophila made their way to the Hawaiian Islands and radiated into over 800 species.[6] They can be found in deserts, tropical rainforest, cities, swamps, and alpine zones. Some northern species hibernate. The northern species D. montana is the best cold-adapted,[7] and is primarily found at high altitudes.[8] Most species breed in various kinds of decaying plant and fungal material, including fruit, bark, slime fluxes, flowers, and mushrooms. The larvae of at least one species, D. suzukii, can also feed in fresh fruit and can sometimes be a pest.[9] A few species have switched to being parasites or predators. Many species can be attracted to baits of fermented bananas or mushrooms, but others are not attracted to any kind of baits. Males may congregate at patches of suitable breeding substrate to compete for the females, or form leks, conducting courtship in an area separate from breeding sites.

Several Drosophila species, including D. melanogaster, D. immigrans, and D. simulans, are closely associated with humans, and are often referred to as domestic species. These and other species (D. subobscura, Zaprionus indianus[10][11][12]) have been accidentally introduced around the world by human activities such as fruit transports.

Side view of head showing characteristic bristles above the eye

Males of this genus are known to have the longest sperm cells of any studied organism on Earth, including one species, Drosophila bifurca, that has sperm cells that are 58 mm (2.3 in) long.[13] The cells are mostly tail, and are delivered to the females in tangled coils. The other members of the genus Drosophila also make relatively few giant sperm cells, with that of D. bifurca being the longest.[14] D. melanogaster sperm cells are a more modest 1.8 mm long, although this is still about 35 times longer than a human sperm. Several species in the D. melanogaster species group are known to mate by traumatic insemination.[15]

Drosophila species vary widely in their reproductive capacity. Those such as D. melanogaster that breed in large, relatively rare resources have ovaries that mature 10–20 eggs at a time, so that they can be laid together on one site. Others that breed in more-abundant but less nutritious substrates, such as leaves, may only lay one egg per day. The eggs have one or more respiratory filaments near the anterior end; the tips of these extend above the surface and allow oxygen to reach the embryo. Larvae feed not on the vegetable matter itself, but on the yeasts and microorganisms present on the decaying breeding substrate. Development time varies widely between species (between 7 and more than 60 days) and depends on the environmental factors such as temperature, breeding substrate, and crowding.

Fruit flies lay eggs in response to environmental cycles. Eggs laid at a time (e.g., night) during which likelihood of survival is greater than in eggs laid at other times (e.g., day) yield more larvae than eggs that were laid at those times. Ceteris paribus, the habit of laying eggs at this 'advantageous' time would yield more surviving offspring, and more grandchildren, than the habit of laying eggs during other times. This differential reproductive success would cause D. melanogaster to adapt to environmental cycles, because this behavior has a major reproductive advantage.[16]

Their median lifespan is 35–45 days.[17]

Lifecycle of Drosophila

Egg

Larva

Pupae (brown specimens are older than the white ones)

Adult D. melanogaster

The following section is based on the following Drosophila species: Drosophila simulans, and Drosophila melanogaster.

Courtship behavior of male Drosophila is an attractive behaviour.[18] Females respond via their perception of the behavior portrayed by the male.[19] Male and female Drosophila use a variety of sensory cues to initiate and assess courtship readiness of a potential mate.[18][19][20] The cues include the following behaviours: positioning, pheromone excretion, following females, making tapping sounds with legs, singing, wing spreading, creating wing vibrations, genitalia licking, bending the stomach, attempt to copulate, and the copulatory act itself.[21][18][19][20] The songs of Drosophila melanogaster and Drosophila simulans have been studied extensively. These luring songs are sinusoidal in nature and varies within and between species.[20]

The courtship behavior of Drosophila melanogaster has also been assessed for sex-related genes, which have been implicated in courtship behavior in both the male and female.[18] Recent experiments explore the role of fruitless (fru) and doublesex (dsx), a group of sex-behaviour linked genes.[22][18]

The fruitless (fru) gene in Drosophila helps regulate the network for male courtship behavior; when a mutation to this gene occurs altered same sex sexual behavior in males is observed.[23] Male Drosophila with the fru mutation direct their courtship towards other males as opposed to typical courtship, which would be directed towards females.[24] Loss of the fru mutation leads back to the typical courtship behavior.[24]

The following section is based on the following Drosophila species: Drosophila serrata, Drosophila pseudoobscura, Drosophila melanogaster, and Drosophila neotestacea. Polyandry is a prominent mating system among Drosophila.[25][26][27][28] Females mating with multiple sex partners has been a beneficial mating strategy for Drosophila.[25][26][27][28] The benefits include both pre and post copulatory mating. Pre-copulatory strategies are the behaviours associated with mate choice and the genetic contributions, such as production of gametes, that are exhibited by both male and female Drosophila regarding mate choice.[25][26] Post copulatory strategies include sperm competition, mating frequency, and sex-ratio meiotic drive.[25][26][27][28]

These lists are not inclusive. Polyandry among the Drosophila pseudoobscura in North America vary in their number of mating partners.[27] There is a connection between the number of time females choose to mate and chromosomal variants of the third chromosome.[27] It is believed that the presence of the inverted polymorphism is why re-mating by females occurs.[27] The stability of these polymorphisms may be related to the sex-ratio meiotic drive.[28]

However, for Drosophila subobscura, the main mating system is monandry, not normally seen in Drosophila.[29]

The following section is based on the following Drosophila species: Drosophila melanogaster, Drosophila simulans, and Drosophila mauritiana. Sperm competition is a process that polyandrous Drosophila females use to increase the fitness of their offspring.[30][31][32][33][34] The female Drosophila has two sperm storage organs that allows her to choose the sperm that will be used to inseminate her eggs.[34] Females have little control when it comes to cryptic female choice.[33][31] Female Drosophila through cryptic choice, one of several post-copulatory mechanisms, which allows for the detection and expelling of sperm that reduces inbreeding possibilities.[32][31] Manier et al. 2013 has categorized the post copulatory sexual selection of Drosophila melanogaster, Drosophila simulans, and Drosophila mauritiana into the following three stages: insemination, sperm storage, and fertilizable sperm.[33] Among the preceding species there are variations at each stage that play a role in the natural selection process.[33]

D. melanogaster is a popular experimental animal because it is easily cultured en masse out of the wild, has a short generation time, and mutant animals are readily obtainable. In 1906, Thomas Hunt Morgan began his work on D. melanogaster and reported his first finding of a white eyed mutant in 1910 to the academic community. He was in search of a model organism to study genetic heredity and required a species that could randomly acquire genetic mutation that would visibly manifest as morphological changes in the adult animal. His work on Drosophila earned him the 1933 Nobel Prize in Medicine for identifying chromosomes as the vector of inheritance for genes. This and other Drosophila species are widely used in studies of genetics, embryogenesis, chronobiology, speciation, neurobiology, and other areas.

However, some species of Drosophila are difficult to culture in the laboratory, often because they breed on a single specific host in the wild. For some, it can be done with particular recipes for rearing media, or by introducing chemicals such as sterols that are found in the natural host; for others, it is (so far) impossible. In some cases, the larvae can develop on normal Drosophila lab medium, but the female will not lay eggs; for these it is often simply a matter of putting in a small piece of the natural host to receive the eggs.

The Drosophila Species Stock Center located at Cornell University in Ithaca, New York, maintains cultures of hundreds of species for researchers.[35]

Drosophila is considered one of the most impeccable genetic model organisms, they have furthered genetic research unlike any other model organisms. Drosophila is a prime candidate for genetic research because the relationship between human and fruit fly genes is very close.[36] Human and fruit fly genes are so similar, that disease-producing genes in humans can be linked to those in flies. The fly has approximately 15,500 genes on its four chromosomes, whereas humans have about 22,000 genes among their 23 chromosomes. Thus the density of genes per chromosome in Drosophila is higher than the human genome.[37] Low and manageable number of chromosomes make Drosophila species easier to study. These flies also carry genetic information and pass down traits throughout generations, much like their human counterparts. The traits can then be studied through different Drosophila lineages and the findings can be applied to deduce genetic trends in humans. Research conducted on Drosophila help determine the ground rules for transmission of genes in many organisms.[38][4] Drosophila is a useful in vivo tool to analyze Alzheimer's disease.[39]

Like other animals, Drosophila is associated with various bacteria in its gut. The fly gut microbiota or microbiome seems to have a central influence on Drosophila fitness and life history characteristics. The microbiota in the gut of Drosophila represents an active current research field.

Drosophila species also harbour vertically transmitted endosymbionts, such as Wolbachia and Spiroplasma. These endosymbionts can act as reproductive manipulators, such as cytoplasmic incompatibility induced by Wolbachia or male-killing induced by the D. melanogaster Spiroplasma poulsonii (named MSRO). The male-killing factor of the D. melanogaster MSRO strain was discovered in 2018, solving a decades-old mystery of the cause of male-killing. This represents the first bacterial factor that affects eukaryotic cells in a sex-specific fashion, and is the first mechanism identified for male-killing phenotypes.[40] Alternatively, they may protect theirs hosts from infection. Drosophila Wolbachia can reduce viral loads upon infection, and is explored as a mechanism of controlling viral diseases (e.g. Dengue fever) by transferring these Wolbachia to disease-vector mosquitoes.[41] The S. poulsonii strain of Drosophila neotestacea protects its host from parasitic wasps and nematodes using toxins that preferentially attack the parasites instead of the host.[42][43][44]

Drosophila species are prey for many generalist predators, such as robber flies. In Hawaii, the introduction of yellowjackets from mainland United States has led to the decline of many of the larger species. The larvae are preyed on by other fly larvae, staphylinid beetles, and ants.

• Low, Wai Yee; Ng, Hooi Ling; Morton, Craig J.; Parker, Michael W.; Batterham, Philip; Robin, Charles (2007). "Molecular Evolution of Glutathione S-Transferases in the Genus Drosophila". Genetics. Genetics Society of America/Oxford University Press (OUP). 177 (3): 1363–1375. doi:10.1534/genetics.107.075838. ISSN 0016-6731.