Prostaglandin-F synthase

In enzymology, a prostaglandin-F synthase (EC 1.1.1.188) is an enzyme that catalyzes the chemical reaction

(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+

prostaglandin-F synthase
Identifiers
EC number1.1.1.188
CAS number55976-95-9
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Thus, the two substrates of this enzyme are (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate and NADP+, whereas its 3 products are (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate, NADPH, and H+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP+ 11-oxidoreductase. Other names in common use include prostaglandin-D2 11-reductase, reductase, 15-hydroxy-11-oxoprostaglandin, PGD2 11-ketoreductase, PGF2α synthetase, prostaglandin 11-ketoreductase, prostaglandin D2-ketoreductase, prostaglandin F synthase, prostaglandin F synthetase, synthetase, prostaglandin F2α, prostaglandin-D2 11-reductase, PGF synthetase, NADPH-dependent prostaglandin D2 11-keto reductase, and prostaglandin 11-keto reductase. This enzyme participates in arachidonic acid metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1RY0, 1RY8, 1VBJ, 1XF0, 1ZQ5, 2F38, and 2FGB.

References
    • Reingold DF, Kawasaki A, Needleman P (1981). "A novel prostaglandin 11-keto reductase found in rabbit liver". Biochim. Biophys. Acta. 659 (1): 179–88. doi:10.1016/0005-2744(81)90282-5. PMID 7248318.
    • Watanabe K, Shimizu T, Hayaishi O (1981). "Enzymatic conversion of prostaglandin-D2 to prostaglandin-F2α in the rat lung". Biochem. Int. 2: 603–610.
    • Watanabe K, Yoshida R, Shimizu T, Hayaishi O (1985). "Enzymatic formation of prostaglandin F2α from prostaglandin H2 and D2. Purification and properties of prostaglandin F synthetase from bovine lung". J. Biol. Chem. 260 (11): 7035–41. doi:10.1016/S0021-9258(18)88884-6. PMID 3858278.
    • Wong PY (1981). "Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver". Biochim. Biophys. Acta. 659 (1): 169–78. doi:10.1016/0005-2744(81)90281-3. PMID 7248317.
    • Wong PY (1982). "Purification of PGD2 11-ketoreductase from rabbit liver". Methods Enzymol. 86: 117–25. doi:10.1016/0076-6879(82)86179-X. PMID 7132748.


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