Surface chemistry of microvasculature

Capillary walls contain of a monolayer of endothelial cells. There are two ways for molecules to diffuse through the endothelial monolayer: through gaps between the cells or directly through the cells. Molecules diffuse through the capillary walls due to concentration gradients. Diffusion between the cells changes depending upon the type of capillary. There are three different types of capillaries: continuous, fenestrated, and sinusoidal also called discontinous. In continuous capillaries the endothelial cells are tightly spaced, allowing only small molecules like ions or water to diffuse through the intercellular clefts (the gaps between the endothelial cells). In fenestrated and sinusoidal capillaries there is more space between the cells, allowing the diffusion of macro-molecules and some proteins. Sinusoidal capillaries have large holes that permit the passage of red and white blood cells.[2] The diffusion of gases and lipid-soluble molecules can occur directly through the endothelial cells, described by Fick's First Law:

${\displaystyle {\bigg .}J=-D{\frac {\partial C}{\partial x}}{\bigg .}}$

Where:

• J is the flux
• D is the diffusivity
• C is the concentration
• x is the thickness of the barrier

Different types of capillaries allow the diffusion of different molecules.

The surface charge of endothelial cells at points of diffusivity can determine which type of molecule can diffuse through the capillary walls. If the surface is hydrophilic, it will allow water and charged molecules to pass through. If it is hydrophobic, non-charged and lipophilic molecules will be able to diffuse through. These intermolecular screening forces are also known as Van der Waals forces, which is determined by the Keesom, Debye and London Dispersion forces. The lipid bilayer of an endothelial cell membrane is a hydrophobic surface. The non-polar lipids lead to a very high electrical resistivity, given by:

${\displaystyle {\bigg .}R_{e}={\frac {R_{m}em}{A}}{\bigg .}}$

Where:

• R_e is the resistivity of the membrane
• R_mem is the specific membrane resistance
• A is the area

This high resistivity prevents ions from crossing the bilayer without an integral membrane protein using facilitated diffusion.[3]

Drugs diffuse through capillary walls in the same manner as endogenous molecules. One of the most important examples of this is drug diffusion across the blood brain barrier. The blood brain barrier consists of a bed of continuous capillaries. Typically only small hydrophobic molecules are able to diffuse across the blood brain barrier.[4] This makes it very difficult to get drugs into the brain without invasively administering them directly into the brain. One possible solution is the utilization of nanoparticles. Nanoparticles are synthesized to encapsulate a target drug. The surface of the nanoparticle, if not already hydrophobic, may have polymers attached to the surface to adjust the current polarity. Ligands can also be attached to the surface of a nanoparticle to target certain receptors located within the brain. Once the nanoparticle is through the blood brain barrier it releases the drug into the brain.[5] A specific example of this solution is the delivery of anti-HIV drugs to the central nervous system by TAT-conjugated nanoparticles.[6]

Vasoconstriction affects flow rate and surface properties of the microvasculature by constricting the smooth muscle cells and decreasing the diameter of flow. The smooth muscle cells are able to constrict from both external and internal stimuli. This mechanism can be triggered by the ambient temperature or the autonomic nervous system.[7] When cold, the body attempts to capture heat in the blood by constricting the smooth muscle cells around the microvasculature. The muscle cells are constricted by an increase in calcium. The decreased cross-sectional area for flow increases the vascular resistance and lowers the flow to extremities. This mechanism allows the body to concentrate the heat around the vital organs for survival.

The formula for calculating the systemic vascular resistance is:

${\displaystyle SVR=80\times {\frac {\left(MAP-MRAP\right)}{CO}}}$

• SVR as the systemic vascular resistance
• MAP as the mean arterial pressure
• MRAP as the mean right arterial pressure
• CO as the cardiac output in milliliters per minute

Typical values are between 100-300 dynes*sec*cm-5.[8]

Vasodilation is, in essence, the opposite of vasoconstriction.[9] In vasodilation the blood vessels dilate to allow more blood flow. The smooth muscle cells are relaxed to increase the diameter of flow, decreasing the vascular resistance. This is possible due to the direct relationship between the cardiac output, mean arterial pressure and the vascular resistance. Like vasoconstriction, vasodilation can be caused by internal and external factors. For example, nitric oxide, found in food, is a very potent vasodilator. It has been found that nerves in the spinal column can trigger both processes. Vasodilation is also triggered for thermoregulation to provide heat dissipation.

Vasoconstriction and vasodilation shown next to a normal capillarity cross section for comparison.

The Starling Equation describes the flow of a solvent through a capillary bed. The equation can be seen below

${\displaystyle J_{v}=K_{f}\left[\left(P_{c}-P_{i}\right)-\sigma \left(\pi _{c}-\pi _{i}\right)\right]}$

A description of the constants, as well as some of the average values can be seen in the following table.

The filtration coefficient is determined by the hydraulic conductivity, which is a measure of how easy it is for water to move through a tortuous area. In microvasculature, this is controlled by the porosity of a capillary and whether it is continuous, fenestrated or sinusoidal. The reflection coefficient acts as a correction factor and is determined by the difference in particle size and pore ratio. It is thought of as the probability that the particle will travel into a pore within the capillary walls before traveling into the interstitial fluid. If the particle is an ion, there will be an effect experienced from the charge of endothelial linings that may increase or decrease the reflection coefficient.

In fluids with relatively low viscosity there is an almost linear, inverse relationship between temperature and surface tension.[13] The decrease in surface tension increases the wettability of the capillary walls, making it easier for the fluid to flow through the capillary.

Heat also effects the viscosity of a fluid inside a capillary. An increase in heat decreases the viscosity of the lumenal fluid. A good example of this action can be observed in the human body during exercise. When a human is exercising, there is an increase in the metabolic rate inside the muscles, creating an increase in heat production. The increase in heat is detected by thermoreceptors, a type of sensory receptor located at various points in body. These receptors send a signal to the brain that tells the body to dilate the blood vessels, including capillaries. This creates a visible change in the number of vessels on the skin. This allows for heat transfer via convection to occur.

Blood vessels transfer heat by convection. Convection is dominated by brownian motion and advection. With convection, the heat in the system moves from a point of high heat concentration to low heat concentration, a concept similar to diffusion. The amount of the heat flow is dependent upon the difference in ambient temperature and internal temperature, which is detected by thermoreceptors. If the ambient temperature is higher than the temperature in the capillaries, the body will open precapillary sphincter to increase the number of capillary beds. This increases the overall capillary surface area, allowing for increased heat transfer through convection.[14]

Surface charges on vessel walls affect the flow of ions through the capillaries and fluid mechanics in microvasculature. A charged endothelial lining creates a bound layer of ions where diffusion occurs between the cell walls and lumen, also known as the stern layer. This creates a diffuse layer, a charge gradient where ions move towards the charged surface, in the endothelial lining of capillaries. The effects of the charged endothelial cells decay exponentially over a measured distance, which is known as the Debye length. Ions in the diffuse layer impinge movement of other ions in the lumen, slowing down the surrounding solution to allow more diffusion to occur. The velocity profile can be determined by the Helmholtz-Smoluchowski[13] equation:

${\displaystyle {\vec {v}}=-{\frac {\epsilon _{o}\epsilon _{r}\zeta {\vec {E}}}{\mu }}}$

• Where ${\displaystyle {\vec {v}}}$ is the velocity
• ε_o is the permittivity of free space
• ε_r is the dielectric constant,
• ζ is the zeta potential
• μ is the viscosity of the fluid
• and ${\displaystyle {\vec {E}}}$ is the electric field created from the charged surface lining of the endothelial cells.

The electric field created by the charged endothelial cells can be conceptually modeled by a hollow cylinder. The charged surface applies a resulting electric field that acts on a particle within the center of a cylinder in a direction perpendicular to the cross section of the observed cylinder.

When there is an increased salt concentration in the lumen of the capillary, the debye length decreases. This decreases the magnitude of the velocity described by the Helmholtz-Smoluchowski equation.