Three-spined stickleback

The three-spined stickleback (Gasterosteus aculeatus) is a fish native to most inland and coastal waters north of 30°N. It has long been a subject of scientific study for many reasons. It shows great morphological variation throughout its range, ideal for questions about evolution and population genetics. Many populations are anadromous (they live in seawater but breed in fresh or brackish water) and very tolerant of changes in salinity, a subject of interest to physiologists. It displays elaborate breeding behavior (defending a territory, building a nest, taking care of the eggs and fry) and it can be social (living in shoals outside the breeding season) making it a popular subject of enquiry in fish ethology and behavioral ecology. Its antipredator adaptations, host-parasite interactions, sensory physiology, reproductive physiology, and endocrinology have also been much studied. Facilitating these studies is the fact that the three-spined stickleback is easy to find in nature and easy to keep in aquaria.[2]

Three-spined stickleback
Scientific classification
Kingdom: Animalia
Phylum: Chordata
Class: Actinopterygii
Order: Gasterosteiformes
Family: Gasterosteidae
Genus: Gasterosteus
Species:
G. aculeatus
Binomial name
Gasterosteus aculeatus
Synonyms
  • Gasteracanthus cataphractus Pallas, 1814
  • Gasterosteus aculeatus algeriensis Sauvage, 1874
  • Gasterosteus aculeatus messinicus Stephanidis, 1971
  • Gasterosteus aculeatus microcephalus (non-Girard, 1854)
  • Gasterosteus algeriensis Sauvage, 1874
  • Gasterosteus argentatissimus Blanchard, 1866
  • Gasterosteus argyropomus Cuvier, 1829
  • Gasterosteus atkinsi Bean, 1879
  • Gasterosteus atkinsii Bean, 1879
  • Gasterosteus bailloni Blanchard, 1866
  • Gasterosteus biaculeatus Mitchill, 1815
  • Gasterosteus biarmatus Krynicki, 1840
  • Gasterosteus bispinosus Walbaum, 1792
  • Gasterosteus brachycentrus Cuvier, 1829
  • Gasterosteus cataphractus (Pallas, 1814)
  • Gasterosteus cuvieri Girard, 1850
  • Gasterosteus Dekayi Ayres, 1855
  • Gasterosteus dimidiatus Reinhardt, 1837
  • Gasterosteus elegans Blanchard, 1866
  • Gasterosteus gymnurus (non-Cuvier, 1829)
  • Gasterosteus hologymnus Regan, 1909
  • Gasterosteus inopinatus Girard, 1854
  • Gasterosteus insculptus Richardson, 1855
  • Gasterosteus intermedius Girard, 1856
  • Gasterosteus islandicus (non-Sauvage, 1874)
  • Gasterosteus leiurus Cuvier, 1829
  • Gasterosteus loricatus Reinhardt, 1837
  • Gasterosteus microcephalus (non-Girard, 1854)
  • Gasterosteus nemausensis Crespon, 1844
  • Gasterosteus neoboracensis DeKay, 1842
  • Gasterosteus neustrianus Blanchard, 1866
  • Gasterosteus niger Cuvier, 1829
  • Gasterosteus noveboracensis Cuvier, 1829
  • Gasterosteus obolarius Cuvier, 1829
  • Gasterosteus plebeius Girard, 1854
  • Gasterosteus ponticus Nordmann, 1840
  • Gasterosteus pugetti Girard, 1856
  • Gasterosteus quadrispinosa Crespon, 1844
  • Gasterosteus quadrispinosus Crespon, 1844
  • Gasterosteus semiarmatus Cuvier, 1829
  • Gasterosteus semiloricatus Cuvier, 1829
  • Gasterosteus serratus Ayres, 1855
  • Gasterosteus spinulosus Yarrell, 1835
  • Gasterosteus suppositus Sauvage, 1874
  • Gasterosteus teraculeatus Lacepède, 1801
  • Gasterosteus tetracanthus Cuvier, 1829
  • Gasterosteus texanus Sauvage, 1874
  • Gasterosteus trachurus Cuvier, 1829
  • Gastrosteus hologymnus Regan, 1909

Description

This species can occasionally reach lengths of 8 cm (3.1 in), but lengths of 3–4 centimetres (1.2–1.6 in) at maturity are more common. The body is laterally compressed. The base of the tail is slender. The caudal fin has 12 rays. The dorsal fin has 10–14 rays; in front of it are the three spines that give the fish its name (though some individuals may have only two or four). The third spine (the one closest to the dorsal fin) is much shorter than the other two. The back of each spine is joined to the body by a thin membrane. The anal fin has eight to 11 rays and is preceded by a short spine. The pelvic fins consist of just a spine and one ray. All spines can be locked in an erect position, making the fish extremely hard to swallow by a predator. The pectoral fins are large, with 10 rays. The body bears no scales, but is protected by bony plates on the back, flanks, and belly. Only one ventral plate is present, but the number of flank plates varies greatly across the distribution range and across habitat types (see below); it is normally higher in marine populations (some freshwater populations may in fact lack lateral plates altogether).[3]

Dorsal coloration varies, but tends towards a drab olive or a silvery green, sometimes with brown mottling. The flanks and belly are silvery. In males during the breeding season, the eyes become blue and the lower head, throat, and anterior belly turn bright red. The throat and belly of breeding females can turn slightly pink. A few populations, however, have breeding males which are all black[4] or all white.[5]

Habitat and distribution
Distribution of Gasterosteus aculeatus (Three-spine stickleback) in the United States, from USGS NAS web site

The three-spined stickleback is found only in the Northern Hemisphere, where it usually inhabits coastal waters or freshwater bodies. It can live in either fresh, brackish, or salt water. It prefers slow-flowing water with areas of emerging vegetation. It can be found in ditches, ponds, lakes, backwaters, quiet rivers, sheltered bays, marshes, and harbours.

In North America, it ranges along the East Coast from Chesapeake Bay to the southern half of Baffin Island and the western shore of Hudson Bay, and along the West Coast from southern California to the western shore of Alaska and the Aleutian Islands. It can be found throughout Europe between 35 and 70°N. In Asia, the distribution stretches from Japan and the Korean peninsula to the Bering Straits.

Its distribution could be said to be circumpolar were it not for the fact that it is absent from the north coast of Siberia, the north coast of Alaska, and the Arctic islands of Canada.

Variation in morphology and distribution

Three subspecies are currently recognized by the IUCN:

  • G. a. aculeatus is found in most of the species range, and is the subspecies most strictly termed the three-spined stickleback; its common name in Britain is the tiddler, although "tittlebat" is also sometimes used.
  • G. a. williamsoni, the unarmored threespine stickleback, is found only in North America; its recognised range is southern California, though isolated reports have been made of it occurring in British Columbia and Mexico;
  • G. a. santaeannae, the Santa Ana stickleback, is also restricted to North America.

These subspecies actually represent three examples from the enormous range of morphological variation present within three-spined sticklebacks. These fall into two rough categories, the anadromous and the freshwater forms.

The anadromous form spends most of its adult life eating plankton and fish in the sea, and returns to freshwater to breed. The adult fish are typically between 6 and 10 cm long, and have 30 to 40 lateral armour plates along their sides. They also have long dorsal and pelvic spines. The anadromous form is morphologically similar all around the Northern Hemisphere, such that anadromous fish from the Baltic, the Atlantic and the Pacific all resemble each other quite closely.

Three-spined stickleback populations are also found in freshwater lakes and streams. These populations were probably formed when anadromous fish started spending their entire lifecycle in fresh water, and thus evolved to live there all year round. Freshwater populations are extremely morphologically diverse, to the extent that many observers (and some taxonomists) would describe a new subspecies of three-spined stickleback in almost every lake in the Northern Hemisphere. One consistent difference between freshwater populations and their anadromous ancestors is the amount of body armour, as the majority of freshwater fish only have between none and 12 lateral armour plates, and shorter dorsal and pelvic spines. However, also large morphological differences occur between lakes. One major axis of variation is between populations found in deep, steep-sided lakes and those in small, shallow lakes. The fish in the deep lakes typically feed in the surface waters on plankton, and often have large eyes, with short, slim bodies and upturned jaws. Some researchers refer to this as the limnetic form. Fish from shallow lakes feed mainly on the lake bed, and are often long and heavy bodied with relatively horizontal jaws and small eyes. These populations are referred to as the benthic form.

Since each watershed was probably colonised separately by anadromous sticklebacks, morphologically similar populations in different watersheds or on different continents are widely believed to have evolved independently. A unique population is found in the meromictic Pink Lake in Gatineau Park, Quebec.

One aspect of this morphological variation is that a number of lakes contain both a limnetic and a benthic type, and these do not interbreed with each other. Evolutionary biologists often define species as populations that do not interbreed with each other (the biological species concept), thus the benthics and limnetics within each lake would constitute separate species. These species pairs are an excellent example of how adaptation to different environments (in this case feeding in the surface waters or on the lake bed) can generate new species. This process has come to be termed ecological speciation. This type of species pair is found in British Columbia. The lakes themselves only contain three-spined sticklebacks and cutthroat trout, and all are on islands. Tragically, the pair in Hadley Lake on Lasqueti Island was destroyed in the mid-1980s by the introduction of a predatory catfish, and the pair in Enos Lake on Vancouver Island has started to interbreed and are no longer two distinct species.[6] The two remaining pairs are on Texada Island, in Paxton Lake and Priest Lake, and they are listed as Endangered in the Canadian Species at Risk Act.[7]

Other species pairs which consist of a well-armored marine form and a smaller, unarmored freshwater form are being studied in ponds and lakes in south-central Alaska that were once marine habitats such as those uplifted during the 1964 Alaska earthquake. The evolutionary dynamics of these species pairs are providing a model for the processes of speciation which has taken place in less than 20 years in at least one lake. In 1982, a chemical eradication program intended to make room for trout and salmon at Loberg Lake, Alaska, killed the resident freshwater populations of sticklebacks. Oceanic sticklebacks introduced through nearby Cook Inlet recolonized the lake. In just 12 years beginning in 1990, the frequency of the oceanic form dropped steadily, from 100% to 11%, while a variety with fewer plates increased to 75% of the population, with various intermediate forms making up another small fraction.[8] This rapid evolution is thought to be possible through genetic variations that confer competitive advantages for survival in fresh water when conditions shift rapidly from salt to fresh water. However, the actual molecular basis of this evolution still remains unknown.

Although sticklebacks are found in many locations around the coasts of the Northern Hemisphere and are thus viewed by the IUCN as species of least concern, the unique evolutionary history encapsulated in many freshwater populations indicates further legal protection may be warranted.[1]

Diet

In its different forms or stages of life, the three-spined stickleback can be a bottom-feeder (most commonly chironomid larvae) or a planktonic feeder in lakes or in the ocean; it can also consume terrestrial prey fallen to the surface.[9] It can cannibalize eggs and fry.[10]

Life history
Male stickleback with red throat and shiny blue eye

Many populations take two years to mature and experience only one breeding season before dying, and some can take up to three years to reach maturity. However, some freshwater populations and populations at extreme latitudes can reach maturity in only one year.

Reproduction
Reproduction of the three-spined stickleback

From late April, males and females move from deeper waters to shallow areas. There, each male defends a territory where he builds a nest on the bottom. He starts by digging a small pit. He then fills it with plant material (often filamentous algae), sand, and various debris which he glues together with spiggin, a proteinaceous substance secreted from the kidneys (the word spiggin comes from spigg, the Swedish name for the three-spined stickleback). He then creates a tunnel through the more or less spherical nest by swimming vigorously through it. Nest building typically takes 5–6 hours[11] though it may also be spread out over several days. After this, the male courts gravid females that pass by with a zigzag dance. He approaches a female by swimming very short distances left and right, and then swims back to the nest in the same way. If the female follows, the male often pokes his head inside the nest, and may swim through the tunnel. The female then swims through the tunnel as well, where she deposits 40–300 eggs. The male follows to fertilize the eggs. The female is then chased away by the male. For the duration of the eggs' development, the male will chase away other males and nongravid females. He may, however, court other gravid females (more than one batch of eggs can be deposited in the same nest).

The sequence of territorial courtship and mating behaviours was described in detail by Niko Tinbergen in a landmark early study in ethology. Tinbergen showed that the red colour on the throat of the territorial male acts as a simple sign stimulus, releasing aggression in other males and attracting females.[12] The red colouration may also be used by females as a way to assess male quality. Red colouration is produced from carotenoids found in the diet of the fish. As carotenoids cannot be synthesised de novo, the degree of colouration gives an indication of male quality (ability to find food), with higher-quality males showing more intense colouration. Also, males that bear fewer parasites tend to exhibit brighter red colours. Many studies have shown that females prefer males with brighter red colouration.[13][14][15][16] However, the response to red is not universal across the entire species,[17][18] with black throated populations often found in peat-stained waters.

The male takes care of the developing eggs by fanning them. He lines himself up with the entrance of the nest tunnel and swims on the spot. The movement of his pectoral fins creates a current of water through the nest, bringing fresh (well-oxygenated) water to the eggs. He does this not only during the day, but throughout the night, as well.[19] Fanning levels tend to increase until the eggs are about to hatch, which takes 7–8 days at 18–20 °C. Fanning levels also increase when the water is poorly oxygenated.[20] Towards the end of the egg development phase, the male often makes holes in the roof and near the rim of the nest, presumably to improve ventilation of the nest during fanning at a time when the eggs are more metabolically active. Once the young hatch, the male attempts to keep them together for a few days, sucking up any wanderers into his mouth and spitting them back into the nest. Afterwards, the young disperse and the nest is either abandoned by the male, or repaired in preparation for another breeding cycle.

In Nova Scotia, a form of three-spined stickleback departs from the usual pattern of parental care. Unlike other sticklebacks that nest on the substrate, Nova Scotian male sticklebacks build nests in mats of filamentous algae. Surprisingly, almost immediately after fertilization, the males disperse the eggs from the nest and resume soliciting females for eggs. Hence, there appears to have been a loss of parental care in this population. Because these males have reduced dorsal pigmentation, resulting a pearlescent white appearance, they have been dubbed "white sticklebacks". It is currently unknown whether they are a distinct species, or simply a morph of the common Atlantic stickleback.[21][22][23]

Infection with the cestode parasite Schistocephalus solidus causes a reduction in egg mass production or complete absence of eggs in female three-spined sticklebacks.[24]

Cooperative behavior

Some evidence indicates the existence of cooperative behavior among three-spined sticklebacks, mainly cooperative predator inspection. Predator inspection appears to allow acquisition of information about the risk a potential predator presents, and may deter attack, with the cost being an increased chance of being attacked if the predator proves to be hungry.

Tit-for-tat strategy

Sticklebacks are known to cooperate in a tit-for-tat (TFT) strategy when doing predator inspection. The idea behind TFT is that an individual cooperates on the first move and then does whatever its opponent does on the previous move. This allows for a combination of collaborative (it starts by cooperating), retaliatory (punishes defection), and forgiving (respond to cooperation of others, even if they had defected previously) behavioral responses.[25] When three-spined sticklebacks approaching a live predator were provided with either a simulated cooperating companion or a simulated defecting one, the fish behaved according to tit-for-tat strategy, supporting the hypothesis that cooperation can evolve among egoists.[26]

Typically, sticklebacks operate in pairs. Individuals have partners with which they repeatedly perform pairwise predator inspection visits. Two reciprocal pairs per trial occur significantly more often than what was expected due to chance. These results provide further evidence for a tit-for-tat cooperation strategy in sticklebacks.[27]

Stickleback behavior is often cited as an archetypal example of cooperative behavior during predator inspection. Fish from three sites differing in predation risk inspected a model predator in pairs and reciprocated both cooperative moves and defections by the partner, but not on every opportunity.[28] Sticklebacks that originated in the two sites containing piscivorous fish were more likely to reciprocate following a cooperative move than following a defection. Individuals from higher-risk sites were generally more cooperative.[28] Individuals accompanied by a model companion show reciprocal moves of cooperation and defection in response to the model's movements about a third of the time. Both examples of stickleback behavior demonstrate the elements of a strategy of cooperation that may resemble tit-for-tat.[28]

Partner-dependence

The tit-for-tat cooperation strategy has been shown to be evident in sticklebacks. In addition, the size of a stickleback's partner fish may also be a factor in determining what a stickleback will do when both fish are faced with a predator. Two sticklebacks simultaneously presented to a rainbow trout, a predator much larger in size, will have differing risks of being attacked. Usually, the larger of the two sticklebacks has a higher risk of being attacked.[29] Individual sticklebacks are more likely to move closer to a trout (or some other predator) when a larger potential partner moves close to the trout than when a smaller partner approaches the trout.[29] Although both large and small partners behave similarly, a small partner's behavior affects the strategy of the test fish more than that of the large partner.[29] Regardless of whether it is alone or with a partner that cooperates, a larger fish will approach a predator more closely than does a smaller fish.[29] If a partner defects, then a stickleback's condition-factor (i.e. its ability to flee) determines how closely it approaches the predator rather than the stickleback's size.[29] Both the strategy and reaction to different-sized partners seem to be dependent on whether the partner cooperates or defects.

Sensory biology
A three-spined stickleback with stained neuromasts that form the lateral line system.

Sticklebacks have four colour photoreceptor cells in their retina, making them potentially tetrachromatic. They are capable of perceiving ultraviolet wavelengths of light invisible to the human eye and use such wavelengths in their normal behavioural repertoire.

Parasites

The three-spined stickleback is a known intermediate host for the hermaphroditic parasite Schistocephalus solidus, a tapeworm of fish and fish-eating birds.[30]

Genetics

Three-spined sticklebacks have recently become a major research organism for evolutionary biologists trying to understand the genetic changes involved in adapting to new environments. The entire genome of a female fish from Bear Paw Lake in Alaska was recently sequenced by the Broad Institute and many other genetic resources are available.[31] This population is under risk from the presence of introduced northern pike in a nearby lake.

References
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  2. Barber, Iain (2013). "Sticklebacks as model hosts in ecological and evolutionary parasitology". Trends in Parasitology. 29 (11): 556–566. doi:10.1016/j.pt.2013.09.004. PMID 24145060.
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  5. Haglund, T. R.; Buth, D. G.; Blouw, D. M. (1990). "Allozyme variation and the recognition of the "white stickleback"". Biochemical Systematics and Ecology. 18 (7–8): 559–563. doi:10.1016/0305-1978(90)90129-4.
  6. Behm, J. E.; Ives, A. R.; Boughman, J. W. (2010). "Breakdown in Postmating Isolation and the Collapse of a Species Pair through Hybridization". The American Naturalist. 175 (1): 11–26. doi:10.1086/648559. PMID 19916869. S2CID 15817509.
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  8. Carroll, Sean B. (2006). The Making of the Fittest: DNA and the Ultimate Forensic Record of Evolution. W.W. Norton & Co. pp. 56–57. ISBN 978-0-393-06163-5.
  9. Sánchez-Hernández, J. (2012). "Aplicación del análisis de los rasgos biológicos ("traits") de las presas para el estudio del comportamiento alimentario en peces bentófagos: el ejemplo del espinoso (Gasterosteus gymnurus Cuvier 1829)". Limnetica. 31 (1): 59–76.
  10. Whoriskey, F. G.; FitzGerald, G. J. (1985). "Sex, cannibalism and sticklebacks". Behavioral Ecology and Sociobiology. 18 (1): 15–18. doi:10.1007/BF00299233 (inactive 2021-01-05).CS1 maint: DOI inactive as of January 2021 (link)
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  12. Tinbergen, N. (1951) The study of instinct, Clarendon Press, Oxford.
  13. Milinski, M.; Bakker, T. C. M. (1990). "Female sticklebacks use male coloration in mate choice and hence avoid parasitized males" (PDF). Nature. 344 (6264): 330–333. Bibcode:1990Natur.344..330M. doi:10.1038/344330a0. S2CID 4322443.
  14. McLennan, D. A.; McPhail, J. D. (1990). "Experimental investigations of the evolutionary significance of sexually dimorphic nuptial colouration in Gasterosteus aculeatus (L.): The relationship between male colour and female behaviour". Canadian Journal of Zoology. 68 (3): 482–492. doi:10.1139/z90-071.
  15. Bakker, T. C. M.; Mundwiler, B. (1994). "Female mate choice and male red coloration in a natural three-spined stickleback (Gasterosteus aculeatus) population" (PDF). Behavioral Ecology. 5: 74–80. doi:10.1093/beheco/5.1.74.
  16. Baube, C.L.; Rowland, W.J.; Fowler, J.B. (1995). "The mechanismsof colour-based mate choice in female threespine sticklebacks: hue, contrast and configurational cues". Behaviour. 132 (13/14): 979–996. doi:10.1163/156853995x00405. JSTOR 4535315.
  17. McKinnon, J. S. (1995). "Video mate preferences of female three-spined sticklebacks from populations with divergent male coloration". Animal Behaviour. 50 (6): 1645–1655. doi:10.1016/0003-3472(95)80018-2. S2CID 53193561.
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  19. Reebs, S. G.; Whoriskey Jr., F. G.; Fitzgerald, G. J. (1984). "Diel patterns of fanning activity, egg respiration, and the nocturnal behavior of male three-spined sticklebacks, Gasterosteus aculeatus L. (f. trachurus)". Canadian Journal of Zoology. 62 (3): 329–334. doi:10.1139/z84-051.
  20. Seventer, P. (1961). "A causal study of a displacement activity (fanning in Gasterosteus aculeatus L.)". Behaviour Supplement. 9: 1–170.
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  23. Jamieson, I. G.; Blouw, D. M.; Colgan, P. W. (1992). "Field observations on the reproductive biology of a newly discovered stickleback (Gasterosteus)". Canadian Journal of Zoology. 70 (5): 1057–1063. doi:10.1139/z92-148.
  24. Heins, David C.; Baker, John A. (February 2003). "Reduction of egg size in natural populations of threespine stickleback infected with a cestode macroparasite". Journal of Parasitology. 89 (1): 1–6. doi:10.1645/0022-3395(2003)089[0001:ROESIN]2.0.CO;2. PMID 12659295.
  25. Nicholas B. Davies; John R. Krebs; Stuart A. West (2012-04-02). An introduction to behavioural ecology (4th ed.). Oxford: Wiley-Blackwell. ISBN 978-1-4051-1416-5.
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  28. Huntingford, Felicity; John Lazarus; Brian Barrie; Sally Webb (1994). "A dynamic analysis of cooperative predator inspection in sticklebacks". Animal Behaviour. 47 (2): 413–423. doi:10.1006/anbe.1994.1055. S2CID 53156278.
  29. Kulling, David; Manfred Milinski (1992). "Size-dependent predation risk and partner quality in predator inspection of sticklebacks". Animal Behaviour. 44 (5): 949–955. doi:10.1016/s0003-3472(05)80590-1. S2CID 53161426.
  30. Lobue, C. P.; Bell, M. A. (1993). "Phenotypic Manipulation by the Cestode Parasite Schistocephalus solidus of Its Intermediate Host, Gasterosteus aculeatus, the Threespine Stickleback". The American Naturalist. 142 (4): 725–35. doi:10.1086/285568. JSTOR 2462871. PMID 19425968. S2CID 40173189.
  31. "Stickleback Genome at ENSEMBL".

Further reading
  • Wootton, R.J. 1976. The biology of the sticklebacks. Academic Press, London.
  • Bell, M.A., and Foster, S.A. (eds.) 1994. The evolutionary biology of the three-spined stickleback. Oxford University Press, New York.
  • Ostlund-Nilsson, S., Mayer, I., and Huntingford, F.A. (eds.) 2007. Biology of the three-spined stickleback. CRC Press, Boca Raton.
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