Tropinone reductase II
In enzymology, a tropinone reductase II (EC 1.1.1.236) is an enzyme that catalyzes the chemical reaction
- pseudotropine + NADP+ tropinone + NADPH + H+
| tropinone reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.1.1.236 | ||||||||
| CAS number | 136111-61-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are pseudotropine and NADP+, whereas its 3 products are tropinone, NADPH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is pseudotropine:NADP+ 3-oxidoreductase. Other names in common use include tropinone (psi-tropine-forming) reductase, pseudotropine forming tropinone reductase, tropinone reductase (ambiguous), and TR-II. This enzyme participates in alkaloid biosynthesis ii.
Structural studies
As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1AE1, 1IPE, 1IPF, 1XHL, 2AE1, and 2AE2.
References
- Drager B, Hashimoto T, Yamada Y (1988). "Purification and characterization of pseudotropine forming tropinone reductase from Hyoscyamus niger root cultures". Agric. Biol. Chem. 52 (10): 2663–2667. doi:10.1271/bbb1961.52.2663.
- Couladis MM, Friesen JB, Landgrebe ME, Leete E (1991). "Enzymes catalysing the reduction of tropinone to tropine and ψ-tropine isolated from the roots of Datura innoxia". Phytochemistry. 30 (3): 801–805. doi:10.1016/0031-9422(91)85255-X.
- Nakajima K, Hashimoto T, Yamada Y (1993). "Two tropinone reductases with different stereospecificities are short-chain dehydrogenases evolved from a common ancestor". Proc. Natl. Acad. Sci. U.S.A. 90 (20): 9591–5. Bibcode:1993PNAS...90.9591N. doi:10.1073/pnas.90.20.9591. PMC 47615. PMID 8415746.
- Drager B (2006). "Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism". Phytochemistry. 67 (4): 327–37. doi:10.1016/j.phytochem.2005.12.001. PMID 16426652.