DHRS7B

The DHRS7B gene is located on the positive strand of chromosome 17, beginning at position 21030258 and ending at position 21094836 (64579 bp).[10] DHRS7B contains seven exons with no predicted alternate splice forms, resulting in an 1841 bp mRNA product.[10][11]

Upstream of DHRS7B on the negative strand of chromosome 17p11.2 are the genes Coiled-coil domain containing 144 family, N-terminal-like (CCDC144NL) and Ubiquitin specific peptidase 22 (USP22).[12] Downstream of DHSRS7B on the negative strand of chromosome 17p11.2 is the gene Transmembrane protein 11 (TMEM11), and on the positive strand is the gene Mitogen-activated protein kinase, kinase 3 (MAP2K3).[12]

Microarray and EST data indicates that the DHRS7B gene is highly expressed in the testes, thyroid, kidneys, and adipose tissues. There is moderate expression in the brain, pancreas, mammary glands, and ovaries. Finally, there is little expression in spleen, thymus, tonsils, bone marrow, and bladder.[13][14]

In humans, DHRS7B has been shown to physically interact with other proteins such as Mediator complex subunit 19 (MED19) and Brain and reproductive expressed-modulator protein (BRE).[19] MED19 was found to interact with DHRS7B through a two hybrid screening approach and plays a role as a co-activator in regulated transcription of most RNA polymerase II dependent genes.[20] BRE is a component of the BRCA1-A complex, which recognizes Lys-63 linked ubiquitinated histones H2A and H2AX DNA lesion sites (identified using anti-tag coimmunoprecipitation).[21] Other proteins interacting with DHRS7B have only been identified through text mining.

Conservation of the DHRS7B protein sequence has been observed highly in mammals; moderately in reptiles, birds, fish and amphibians; minimally in invertebrates, insects, and fungi.[22]

Paralogs of DHRS7B are all in the SDR superfamily and conservation of the SDR functional motifs was identified in a multiple sequence alignment.[22][23]