List of Spider-Man (1994 TV series) characters

Peter Benjamin Parker (voiced by Christopher Daniel Barnes) is the main protagonist of the series. A one-time high school "science geek", Peter was the subject of much ridicule, especially at the hands of Flash Thompson. After his parents died in a plane crash, Peter lived with his Uncle Ben and Aunt May, who doted and worried endlessly about the reclusive boy suddenly placed in their midst. During a class field trip to see a demonstration of Neogenic radiation, carried on by Professor Farley Stillwell, a spider crawled into Stillwell's machine and became irradiated, biting Peter in the hand and granting him the proportionate abilities of a spider, including superhuman strength, durability, stamina, speed, agility, senses and a "Spider-Sense" that warned him of imminent danger.

Peter initially became Spider-Man for personal reasons, seeking the popularity that he never had at school. Constructing mechanical web-shooters in his bedroom, Parker set out to make a name for himself in show-business, making television appearances and starting a brief career as a professional wrestler.

It was after one such wrestling engagement that Peter's life changed for the worse; fame went to the idealistic Parker's head, and he refused to stop an escaping criminal, considering it "none of his business". A short time later, Parker returned home to find that his uncle Ben had been shot dead by a home invader, who had then escaped to an abandoned waterfront warehouse. Following the murderer, Parker was shocked and ashamed to discover that his uncle's death had been his fault; his uncle's killer was the same man that Parker had let escape earlier in the day.

Devastated by his own actions, Peter turned Spider-Man into a force for good, promising to protect the city and its occupants. In his civilian life, Peter got a job as a photographer at the Daily Bugle, working for J. Jonah Jameson (a man who passionately despises Spider-Man), and attending Empire State University as a college student. Spider-Man once told a little fangirl of his named Tiana about his origins (albeit not revealing his secret identity) and after she helped him defeat Dr. Octopus in a battle, he willingly revealed his identity to her - and unbeknownst to him, Madame Web had him do this because Tiana was terminally ill.

Over the years, Spider-Man has gathered an impressive gallery of villains, including such notables as the Kingpin, Mysterio, Dr. Octopus, and the ever-unpredictable alien symbiote known as "Venom". Three villains have discovered Spider-Man's civilian identity, either by accident or via prior knowledge (the Venom symbiote originally bonded with Spider-Man, and therefore knew of his secret identity by the time it found Eddie Brock. The first Green Goblin followed him long enough to see Peter take off his mask in an alley. And the second Goblin had gotten this knowledge from the first through a spiritual link after the first got into a dimensional portal after a battle with Spider-Man, but twice, did the second Goblin gain and lose this knowledge through amnesia). Spider-Man would be bearhugged by the Scorpion ("the Sting of the Scorpion"), Tombstone ("Ravages of Time"), Alistair Smythe as the Ultimate Spider-Slayer Cyborg ("the Ultimate Slayer") and most of all the Kingpin ("the Spot" and "Goblin War!").

Peter would later discover that his parents were, in fact, spies for the American government, but were branded as Soviet double agents against the United States. However, he did manage to prove their innocence in the longest episode arc, the five-part "Six Forgotten Warriors" saga. During the course of the series, Peter would also marry his longtime, on-and-off love, a clone of Mary Jane Watson (unbeknownst to him until minutes before her death), and briefly dated the real Mary Jane before she disappeared into limbo during a pitched battle against the Green Goblin (she fell into a dimensional portal because of a machine the Green Goblin had that created portals leading into other dimensions called the Time Dilation Accelerator).

By the close of the series, Peter had been chosen by Madame Web and the Beyonder to prevent the creature known as Spider-Carnage from destroying all of reality, who was living in a different reality opposite from Peter's, leading a whole group of other Spider-Men (all played by Christopher Daniel Barnes including himself in the show) from different realities to go against Spider-Carnage, including the Scarlet Spider, who was Spider-Carnage's enemy. In defeating Spider-Carnage using the uncle Ben who was alive in another reality that Peter and Spider-Carnage went into (it belonged to one of the Spider-Men who was rich and had his identity public), Madame Web fulfilled her promise to help Spider-Man find the real Mary Jane.

Eric Brooks (voiced by J.D. Hall) first appears in the episode entitled "Neogenic Nightmare Chapter 9: Blade the Vampire Hunter." In that episode, Spider-Man and Blade first meet each other, and it also marked the very first appearance of Whistler in any version of the Marvel Universe, as Whistler had never appeared in a comic book before. In this episode, Blade comes to New York to hunt down Morbius, after realizing it was not Spider-Man, who he was originally targeting, who was sucking the blood out of the citizens of New York. It was also revealed that Blade was the son of a vampire man with a human mother, but became orphaned after his mother left him in a foster home after she realized she, too, would become a vampire from her mate. Whistler, who was a vampire hunter at the time, had discovered Blade with his unique potential of having all the strengths of a vampire but none of their weaknesses, except for the thirst of human blood (plasma, as it is called in the series for censorship reasons). Whistler took Blade under his care, trained him to use his vampire powers for good and helped Blade's vampire urge for getting human blood by giving him a serum which would make him more human, but only if he wanted to be more human, and the serum always worked. By the end of the episode, after Blade was almost killed by Morbius in confrontation if it were not for Spider-Man, the latter teamed up with Blade so they could stop Morbius into turning all humans into vampires (Morbius told Blade this at the start of their confrontation).

In the following episode "The Immortal Vampire", Blade once again makes an appearance alongside Spider-Man. Morbius attempts to use the Neogenic Recombinator to turn everyone else into a vampire. Spider-Man and Blade manage to stop him (Morbius' love, Felicia Hardy, persuaded Morbius not to turn her into a vampire and for him to become a human again, but Spider-Man and Blade accidentally directed the active Recombinator at Felicia, but Morbius had thrown himself in the direction and became a full, incurable vampire). Blade also found a love interest in Police Lieutenant Terri Lee, who was a mutual ally to Spider-Man. In the show's fourth season, in the episode "Partners in Danger: Chapter 7: The Vampire Queen", Blade returns and hunts down the Vampire Queen in Eastern Europe, as explained by Whistler to the Black Cat in the previous episode, The Awakening, who is revealed to be Blade's mother. Blade made his final appearance in the show in the 5th-season episode "Secret Wars: Chapter 2: Gauntlet of the Red Skull" where he was seen working side by side with Morbius and the Black Cat against the Vampire Queen until the Cat was "abducted" by Spider-Man, who used a teleportation device from an alien world, to help him in his battle against evil on his side of good in the Secret Wars. It should also be noted that in this series, Blade wore a costume based on his appearance in the Nightstalkers comic series and his subsequent solo series. In this series, he also does not wear his trademark sunglasses and his sword has an energy blade.

Felicia Hardy (voiced by Jennifer Hale) and her alter-ego, Black Cat, were depicted as the first potential love interests for both Peter Parker and Spider-Man, respectively, rivaled only by Mary Jane Watson. Felicia was the well-bred, well-to-do daughter of business woman Anastasia Hardy, and had only vague memories of her father, John Hardesky, a career jewel thief known as the Cat, who had been imprisoned for years in the top secret and guarded prison of S.H.I.E.L.D. because he had memorized the World War II super soldier formula, which would fall into enemy hands if he was kept free. As Felicia's civilian identity, she is a slightly petite (unlike her alter-ego) but nonetheless she is a very beautiful blonde girl with a crisp brogue and a sharp mind. In an episode where there was a charity ball, she danced with Peter and even kiss him. She briefly dated and fell in love Michael Morbius. Morbius ended up becoming a vampire during a science experiment and disappeared. After Spider-Man rescued her mother from the Green Goblin, Felicia fell in love with him. Then she realized that she could not have a relationship with Spider-Man, she dated another man named Jason Philips. The latter was later revealed to be the Hobgoblin, ending their relationship when he was sent to prison. The revelations left her devastated.

In the fourth season "Partners in Danger" arc, Black Cat was finally introduced, as Wilson Fisk, the Kingpin of crime (whose civilian identity regularly worked with Anastasia Hardy), learned of John Hardesky memorizing the supersoldier formula and had him abducted from the prison known as S.H.I.E.L.D., the Kingpin sent Dr. Octopus (who was blackmailing Anastasia at the time with Hardesky's criminal past) to kidnap Felicia and threatened John if he did not reveal the super soldier formula, Felicia would die. Hardesky told the Kingpin about the formula and it was tested on Felicia to make sure John was not lying. The formula gave her great physical strength, agility and dexterity, and the ability to alter her physical appearance (i.e. hair color to white and costume change, thus transforming her into the more recognizable "comic" form; Black Cat). She was originally a crook for the Kingpin to do his bidding, but soon teamed up with Spider-Man and broke her father free from the Kingpin, putting Hardesky back into S.H.I.E.L.D. on John's will, unbeknownst to either Felicia or her mother (the knowledge from Hardesky was kept secret with Spider-Man). Spider-Man and Black Cat were partners for the following four episodes until the fourth season's seventh episode, where she teamed up with Blade and Morbius to track down and kill Blade's vampire mother, Miriam. Black Cat returned three times after this, first in the fifth-season premiere, attending Peter Parker's wedding with the clone of Mary Jane Watson. In the process, she helped Spider-Man and Kingpin (now controlling a robot called the Mega Slayer) defeat Goblin Warriors that were created by Alistair Smythe. In her second return appearance, she helped Spider-Man save Mary Jane's clone from the clone of Hydro-Man in the two-part The Return of Hydro-Man episode. At this point, she explains to Spider-Man that she came back because she needed a break from vampire hunting and after Mary Jane was rescued, she says that she must return to Morbius and hunt down Blade's mother and that it is where she is needed. She then takes off to return to Europe. Her final appearance was in the final two episodes of the three-part Secret Wars, joining Spider-Man's team as they fought the Red Skull, Dr. Doom and other villains. In the process, she met Captain America, sharing the origins of her powers (but not her identity) with him. In the end, she returns her feelings back towards Spider-Man. After the Secret Wars ended, she rejoined Blade and Morbius with no memory of her experiences in the Secret Wars, like all the other heroes involved (except for Spider-Man for a much bigger purpose, see above). This means that she ends up with Morbius. But she is still an ESU student at New York and has friends that believe she is just overseeing her mother's corporation in Europe for a few weeks. Therefore, it is very likely that she will return to New York with Morbius after Mirium is killed.

Matthew "Matt" Michael Murdock (voiced by Edward Albert), the son of prize-fighter Jonathan "Jack" Murdock, was shocked to discover that his father had worked a deal with Wilson Fisk, the Kingpin of Crime. Running from his father in shame, the devastated youth was almost killed by a chemical truck hauling radioactive waste, which splashed into his eyes and blinded him. His father swore revenge against the Kingpin, for it was the Kingpin's truck that had almost killed Matt; however, the crime lord learned of Jack's intentions and had him killed before he could reveal the information to the authorities, for it was illegal to carry radioactive waste in New York.

Wanting revenge on the Kingpin, much as his late father, Matt discovered that his blindness somehow heightened his senses to superhuman levels, allowing him to navigate with ease in even the most unfamiliar environments.

Training with his mentor, a man known only as "Stick", Matt took on the identity of Daredevil, the "Man Without Fear", a vigilante who fought crime and whose primary priority was to defeat the Kingpin and bring down his criminal empire. Taking up the legal profession of an attorney, Murdock moved to New York's "Hell's Kitchen", a dingy, drug-strewn ghetto, bringing Daredevil's fight for justice to the place where justice was needed most.

Matt Murdock eventually took on a "very" famous client; Peter Parker, secretly known as Spider-Man, whom the Kingpin had set up. Teaming up to clear both Peter and Spider-Man's names, Daredevil vowed to Spider-Man, who he revealed to him that Wilson Fisk was the Kingpin, that he would not rest until the Kingpin was brought to justice. Though the Kingpin himself escaped, Daredevil still roams the rooftops and alleys of Hell's Kitchen, making certain that the corrupt never again pray on the weak.

The Daredevil character only made two appearances in the series; first, in the two-part third-season episode, Framed, and then in The Man Without Fear; despite his infrequent appearance in the series, he has an important role in the Kingpin's past, and even revealed the Kingpin's true identity to Spider-Man, for in the past episodes, Spider-Man never knew that Wilson was the Kingpin, hence, making his appearance a grand turning point in the series.

Colonel Nicholas "Nick" Joseph Fury (voiced by Philip Abbott in "Day of the Chameleon", Jack Angel in later episodes), an eye patch wearing war veteran thought to have been killed in combat, became the leader of the secret police organization S.H.I.E.L.D., he and the rest of S.H.I.E.L.D. were involved in such adventures with Spider-Man such as stopping the Chameleon from killing two international delegates from signing a peace treaty, fighting against the Kingpin's forces to reacquire John Hardesky for Hardesky's super soldier formula knowledge and trying to stop the Kingpin and his rebranded Insidious Six from acquiring the Red Skull's Doomsday Device. The villain Electro destroyed S.H.I.E.L.D. during the five-part Six Forgotten Warriors story arc; Nick's whereabouts and present condition are unknown.

In this series, Sergei Kravinoff (voiced by Gregg Berger) has a more heroic personality than he did in the comics. He made his debut in the episode Kraven the Hunter. He has a fiancée named Dr. Mariah Crawford. Sergei became Kraven the Hunter when Sergei saved Mariah from a group of jungle cats, and Sergei was seriously wounded. Crawford uses an immediate-health serum on him, given to her by a former colleague of hers who was mutated while missing in Africa, to save his life, which causes him to become insane, and giving him extraordinary strength and agility in the process. Mariah moved back to New York City to work on a cure for the serum but Sergei tracked her down, believing Crawford was cheating on him with another man. Mariah teamed up with Spider-Man to cure Sergei but Sergei even kidnapped Joseph Robbie Robertson, a friend of Spider-Man's, because Sergei believed Robertson was the man Mariah was cheating on him with. But Sergei was cured by the end of the episode by Spider-Man to prevent him from attacking innocent people.

In Morbius, he makes a cameo when Mariah calls him from New York to Africa about Spider-Man having his disease mutated. In Duel of the Hunters, a mutative disease turned Spider-Man into a spider-like monster which went on a Hulk-like rampage, and Kraven was sent to track him down. His hunt was initially interrupted by the Punisher, who wanted to kill Spider-Man, but they eventually worked together to track down Spider-Man and restore his human form.

In The Return of Kraven, he came back to New York to cure Mariah when Sergei gave the serum that made him become Kraven to his love because Mariah was dying from a disease that was plaguing Africa, turning her into Calypso. Sergei became Kraven again and tracked down Calypso throughout New York, with Spider-Man and newcomer superhero known as Black Cat on his tail, thinking he is Calypso. But when Empire State University students Debra Whitman and Flash Thompson figured out Calypso's DNA, in which it was female, Spider-Man and Black Cat figured out about this and heard Kraven's story. The three of them teamed up and when they gave Calypso the curing serum, it only half-cured her, leaving her somewhere in between Dr. Mariah Crawford and Calypso. When Spider-Man wanted to cure her all the way back to Mariah, Mariah herself refused and she and Sergei ran off somewhere in an undisclosed location, where their fates are unknown.

Curt Connors (voiced by Joseph Campanella) first appeared in the episode "Night of the Lizard". Here, the Lizard was portrayed as possessing a genius level of intelligence but also having the savage mindset from the comics. His initial plot in his first appearance was to make the Neogenic Recombinator turn everyone around the world into humanoid lizards like him, but Spider-Man stopped him and reused the Recombinator to turn the Lizard back to Dr. Connors. The Lizard later went on to appear in the episodes "Tablet of Time", "Ravages of Time", "The Final Nightmare", "The Lizard King" and the "Secret Wars" 3-part episode from the end of this series' run in 1997. The non-mutated Curt Connors had many other appearances as a supporting character. In the Lizard's four appearances between "Night of the Lizard" and the "Secret Wars" 3-parter portray him as villainous and much more of a brute, even lacking signs of his intelligence that was displayed back in the pilot episode. But it is the Lizard's alter-ego's, Dr. Connors, appearances as a mild-mannered and well-respected, innocent scientist who plays an important role in some of Spider-Man's adventures, notably in Season 2 with the superhero's Neogenic disease, that makes the Lizard an ally, including his appearance in the 3-part "Secret Wars" episode. Dr. Connors/Lizard is a Dr Jekyll and Mr Hyde-type villain, one half a well-respected scientist, the other half a monster.

Dr. Stephen Vincent Strange, M.D. (voiced by John Vernon) appeared in the episode "Doctor Strange". Stephen and his sidekick, Wong, help Spider-Man rescue Mary Jane from Baron Mordo and Dormammu. After that, Stephen detected the presence of Madame Web. Stephen's origin dates back before his debut in the series. He used to be an expert surgeon and when he got into a car accident, he lost the ability to use his hands. He then went to a mystic figure known as the Ancient One, expecting that the Ancient One could help him out with his problems. When the Ancient One refused to do so, Stephen was left as a lonely and broken man but when he figured out the Ancient One's student, Baron Mordo, was planning to assassinate the mystic figure, Stephen tried to warn the Ancient One, but Mordo prevented him. But surprisingly, the Ancient One knew of Mordo's plan all along and foiled it, exiling Mordo for his assassination attempt. But despite Stephen's failure to warn the Ancient One, the Ancient One granted him mystic powers for being noble enough as to at least try to warn the One.

Wong (voiced by George Takei) appeared along with Stephen Strange, in the episode "Doctor Strange" as Stephen's sidekick. Wong wielded twin swords that were mystically enhanced.

Madame Web (voiced by Joan Lee) appeared in the Spider-Man animated series. On the cartoon, she had a recurring role in the third and fifth seasons, giving Spider-Man cryptic yet always vital advice in his adventures and battles against his foes and eventually led him to the Beyonder. Instead of being a mutant, Madame Web was a cosmic entity of great mystical power (dwarfing Stephen Strange's powers). So apparently, she had no human origin.

In the series, Web first appeared in a cameo in the third season's premiere episode, "Doctor Strange", where she spied on Spider-Man, Mary Jane, Stephen Strange and Wong after MJ was saved from Dormammu and Baron Mordo, and Stephen even sensed Web's presence. She made a full appearance in the next episode, "Make a Wish", introducing herself and revealing her knowledge of Spider-Man's life and secret identity. In the series, her role was typically to act as cryptic adviser to Spider-Man, offering him strange clues and riddles that would ultimately help him, as well as supposedly training him for the upcoming "Ultimate Battle". Spider-Man's relationship with Madame Web was reluctant as he wanted to live his life autonomously, whereas she insisted he would need her knowledge for the upcoming difficulties. The pair did separate for a while because when Spider-Man was going to quit in the third-season finale (like usual), Web warned him that the two-headed monster will rise above from the underworld. What Web meant by that was that his enemy, the Green Goblin, would figure out his secret identity, and Spider-Man knew the Goblin was Norman Osborn, and the two identities would clash. When Web refused to bring Green Goblin and MJ from limbo, where they both got stuck into thanks to the Green Goblin's portal making Time Dilation Accelerator machine, under Spider-Man's insistence, that is when they separated. But she returned when the "Ultimate Battle" (analogous to the Secret Wars) occurred, which was after Spider-Man's wife, Mary Jane's clone, had died off (due to her unstable cell structure which caused her to evaporate). After Spider-Man had won in the battle against evil in the entire three-part Secret Wars episode, this ultimately led to the two-part series finale of the show, Spider Wars, where it is revealed the "Secret Wars" was merely a test for Spider-Man to see how formidable for the "real" challenge he would be when he would have to lead Spider-Men from other dimensions to stop an insane Spider-Man villain known as Spider-Carnage from destroying all of reality. Spider-Man succeeded and Web carried out her promise to Spider-Man they would find Mary Jane throughout limbo.

Michael Morbius (voiced by Nick Jameson) first appeared in his human form in "Insidious Six" two-part episode. In these episodes, he has a romance with Felicia Hardy (a.k.a. Black Cat). In the episode entitled "Morbius", he stole a vial of Peter Parker's blood in the belief that this was part of Peter's entry in a science competition they were both competing in. While examining the blood in his laboratory, one of the vampire bats he was experimenting on escaped, and began to lap it up. When Michael tried to scare the bat away, it bit him. The bite infected him with Peter's irradiated blood, transforming him into a living vampire.

He appeared as a villain in the following four episodes, Enter the Punisher, Duel of the Hunters, Blade the Vampire Hunter and The Immortal Vampire, along with "The Awakening" and "The Vampire Queen" in Season 4. In his earlier appearances, he goes on a rampage to turn everyone into Vampires. However he was stopped by Spider-Man and Blade in The Immortal Vampire. At the end of "The Vampire Queen", Morbius stopped becoming an evil blood sucker thanks to a serum created by Abraham Whistler which suppresses anyone who has a vampiric urge for human blood, but only if they wanted to be human and joined forces with vampire hunter Blade and superhuman Black Cat, who is Felicia's alter-ego that Morbius figured out in The Awakening, to stop Blade's evil mother, Mirium. Morbius made a cameo at the beginning and end of the second episode of the animated version of "Secret Wars", along with Blade, who were not only concerned about stopping Mirium but curious where Black Cat went (she was transported from Earth to the Secret Wars planet to fight alongside Spider-Man as one of the heroes to help him). That was the last appearance of Morbius in the show.

A notable thing about Morbius is that the censorship for the show did not allow vampires to appear. So to allow the use of Morbius, he could not bite victims to drink their blood. Despite having fangs, and evidently being a vampire, he instead had to drain victims through blood suckers on his hands. And blood is only referred to as "plasma". The word "blood" is commonly used in non-vampire episodes. Though, the words "blood-bath","blood sample" and so on are used throughout the series (including vampire episodes). Also, in "The Immortal vampire", Morbius says to Peter:"I need a sample of the neogenic blood you created".

Frank Castle (voiced by John Beck) used to be a police officer. Frank's wife and son were shot dead by a group of evil gangs and this caused Frank to seek vengeance towards criminals by killing every single one of them he could find around the world, with the help of his computer sidekick, Microchip. Frank believes he is not bringing himself vengeance, but punishing those who do wrong (hence the Punisher). Frank first appeared in the series when he believed Spider-Man was a blood-sucking vampire who kidnapped Empire State University student Michael Morbius, while Morbius was ironically the vampire all along. Frank had tried his best to hunt down and capture a mutated, six-armed Spider-Man. Suddenly, Spider-Man had mutated into the Man-Spider due to his life evolution, a giant man-sized spider and then Frank was even more determined to kill Spider-Man now, as well as with a challenge from Sergei Kravinoff. Eventually, Sergei and Frank teamed up to cure Spider-Man and Frank called off his manhunt for the persecuted superhero.

Later Frank returned in Season 4 when he felt like killing every single criminal was not working for his life and he wanted to do something good in one innocent person's life. Opportunity knocked for him and approached Anna Watson who was missing her niece, Mary Jane, who had been gone for some time in the series. Anna blamed Mary Jane's boyfriend, Peter Parker, for her disappearance. Frank began looking for Mary Jane with Peter at the top of his suspect list, tracking him down wherever he went. Frank then tracked the Green Goblin (Harry Osborn) but could not find out what he knows about Mary Jane's disappearance as Harry was too insane. But ironically at the time when Peter got back into his house and Frank got him cornered, the clone of Mary Jane appeared, even though no one realized it was not the real MJ, and after a short reunion between Peter and Mary Jane's clone, Frank gave the clone to Anna and he left, feeling good on the inside of himself now.

Whistler (voiced by Malcolm McDowell in the first two appearances, Oliver Muirhead in later appearances) is a former vampire hunter. When he went to New York City one time, he discovered a lost child who is a human-vampire hybrid who has all of the strengths of a normal vampire but none of their weaknesses, except for their thirst of human plasma. Whistler then took the child under his wing, called him Blade and trained him to use his powers for good and hunt and kill vampires as he grew up, as well as giving him a serum that would suppress him of his vampire thirst for blood, but only if Blade wanted to. He told him how he discovered Blade to Spider-Man and detective Terri Lee in the show. Whistler had appeared first in the series in the episode, Blade the Vampire Hunter (which was also Blade's debut in the show) and last appeared in The Vampire Queen which told that Blade's mother is really now the evil vampire queen of Earth.

Whistler was an original character of Spider-Man: The Animated Series, never having appeared in the primary Marvel Universe. He would later be portrayed by Kris Kristofferson in the live-action film trilogy: Blade, Blade II and Blade: Trinity.

Tony Stark (voiced by Robert Hays) first appeared in the episodes "Venom Returns" and "Carnage" in which Dormammu orders Venom to steal the Time Dilation Accelerator from Stark Enterprises, which is capable of releasing Dormammu from his own far-off dimension. When Rhodey is too wounded to continue fighting, Tony teams up with Spider-Man and stops the Symbiotes and prevent Dormammu from leaving his dimension. Tony also makes a cameo in the episode called "The Spot, in which Tony fires Dr. Jonathon Ohn from the Time Dilation Accelerator project because Tony knows the project is dangerous after Carnage almost released Dormammu to Earth using Accelerator machinery. Tony later appears in the three-part episode Secret Wars in which the Beyonder creates a war between good and evil to see who is better. In the end, the heroes win and everyone, except for Spider-Man who has to stop the evil Spider-Carnage from destroying all of reality in the following series finale, is sent back to Earth without any memory.

Rhodey Rhodes (voiced by James Avery) is a friend of Tony Stark. Rhodey first appeared in the episodes "Venom Returns" and "Carnage" in which Dormammu orders Venom to steal the Time Dilation Accelerator from Stark Enterprises, which is capable of releasing Dormammu from his own far-off dimension. Venom is quickly defeated by Spider-Man and War Machine. However, Venom gets help from Cletus Kasady, his cellmate who has bonded with another symbiote, Carnage. After the Symbiotes steal the machine, Rhodey is too wounded to continue fighting, so Tony teams up with Spider-Man and stops the Symbiotes and prevent Dormammu from leaving his dimension.

The X-Men from X-Men: The Animated Series appeared in the crossover episodes "The Mutant Agenda" and "Mutants' Revenge". Cyclops, Wolverine, Rogue, Storm, Beast, Gambit, Jubilee, Jean Grey, and Professor X were featured as the X-Men. In the "Mutant Agenda" episode, Spider-Man's progressing neogenic mutation is making him ill and he seeks the aid of Professor Charles Xavier. In doing so he meets the X-Men but is disappointed to learn that Xavier does not 'cure' mutancy, only helps those born that way to accept and control their abilities. Wolverine in particular took issue with Spider-Man viewing his mutation as a curse. Beast however was more sympathetic to the super-hero's plight and attempted an olive branch towards him. This meeting of heroes was soon followed by a plot by Dr. Herbert Landon and the Hobgoblin to destroy mutants everywhere. But Spider-Man and the X-Men work together to defeat them and end their plot.

Later, Storm appeared in the "Secret Wars" battle on the good side against the evil side.

Captain America (voiced by David Hayter) first appeared in The Cat episode as a cameo, in which Peter Parker is narrating a flashback scene about how Captain America got his powers while reading an article of John Hardesky. Later, Captain America (voiced by David Hayter) appeared in the five-part "Six Forgotten Warriors" episode. In this episode it is revealed that after Steve Rogers was turned into Captain America, the lab was blown up by a Nazi spy and the key scientist was killed in the explosion. However, the remnants were enough to create at least five more superhumans. But their powers were temporary. So they were given rings that would keep their powers permanent, as long as they kept them on. Black Marvel (voiced by Paul Winfield), Miss America (voiced by Kathy Garver), Whizzer (voiced by Walker Edmiston), Destroyer (voiced by Roy Dotrice), and Thunderer (voiced by Hansford Rowe) were the five superheroes created. In this episode, the Kingpin steals the rings from the five heroes to use the Red Skull's doomsday device. The Chameleon tricks the Kingpin and releases the Red Skull and Captain America, who were trapped in a time dilation loophole and never aged a day because of it. Later, the Red Skull unleashes the Doomsday Device by turning his son Kragov into Electro. The Six Warriors reunite, along with Spider-Man, and defeat Electro and the Red Skull. However at the end of the five-parter Captain America, Electro, and Red Skull get trapped in the time dilation loophole, again. Later Captain America appeared in the three part episode Secret Wars in which Spider-Man choose Captain America along with some other heroes to defeat the villains involved in the Wars, which also included the Red Skull.

The Fantastic Four guest starred on the three part episode, Secret Wars, in which the Beyonder forces a team of heroes to battle a team of villains to see who is stronger: good or evil. The Beyonder had chosen Dr. Doom to be one of the villains, so Spider-Man chose the Fantastic Four since they had a past with Dr. Doom enabling the computer to bring Mister Fantastic (voiced by Cam Clarke), Invisible Woman (voiced by Gail Matthius), Human Torch (voiced by Quinton Flynn), and Thing (voiced by Patrick Pinney) to the planet.

At the end of the story, after the heroes won, the Beyonder sent the Fantastic Four back without any memories of the Secret Wars, which had occurred for every other person involved in the Wars except for Spider-Man, who had to deal with the evil Spider-Carnage's plans of destroying all of reality in the following series finale.

Baron Mordo (voiced by Tony Jay) first appears in the Spider-Man: The Animated Series episode Sins of the Fathers Chapter 1: Dr. Strange. In the show, Mordo appears as Dormammu's minion. In his first appearance, he was ordered by Dormammu to steal the Wand of Watoomb to make a portal that would release him from his dimension. To do so, Mordo brainwashes Mary Jane Watson to become his servant. His plan almost succeeded, but Spider-Man, Dr. Strange, his assistant Wong, and Mary Jane stopped him from freeing Mordo's master from his dimension. Mordo's origin is also revealed in this episode where he was once a student under the mystic figure known as the Ancient One's teachings. But when Mordo tried to kill the One, which was discovered by Strange who attempted to stop Mordo but Mordo in turn had stopped Strange, the Ancient One foils Mordo's assassination attempt and expels him, revealing that he had known about Mordo's plan all along.

Later, in "Venom Returns" and "Carnage", Mordo was ordered by Dormammu to bring the Venom symbiote back to Earth to make Eddie Brock whole once more. Then, Dormammu orders Mordo to give Brock a message. At Stark Enterprises, Mordo disguises himself as Orden Bloom in order to get the portal. When Spider-Man and War Machine join forces to fight Venom, Dormammu tells Mordo that there is another symbiote, which Mordo then bestows to Cletus Kasady, naming the result into the evil "Carnage."

After retrieving the probe, Dormammu orders Carnage to steal the "life force" energy from humans, so he may gain enough strength to enter our world. When Carnage absorbs too much force, Mordo brings him back to his lair to drain the power into an urn. When Carnage moves to send the urn into Dormammu's portal, Spider-Man, Iron Man, and Venom interrupt. Mordo ordered Carnage to throw the urn to unleash Dormammu, but was thwarted. Sacrificing himself, Venom pulled Carnage into the dimension following Dormammu's reversion, after which Mordo made his escape.

The Big Wheel (voiced by Michael Des Barres) appeared in episode named "Rocket Racer"; his real name is Jackson Weele. Weele is the head of a band of high-tech thieves, whose technology is stolen by the Rocket Racer, so Weele goes after Rocket Racer. It takes the combined power of Spider-Man and the Rocket Racer to stop the Big Wheel and put him behind bars.

The creation of Carnage was orchestrated by the evil demon Dormammu. The Venom symbiote had previously been launched into space aboard a probe by Spider-Man, but, learning that it was on the verge of asexually reproducing, Dormammu had Baron Mordo bring the probe back to Earth. When the recently arrested Cletus Kasady (voiced by Scott Cleverdon) witnessed Brock's reunion with his symbiote, he was soon offered the chance to merge with the symbiote's spawn. Becoming Carnage, he was sent out to capture enough "life energy" from human beings to provide Dormammu the strength to enter our world. Because of the degree of censorship placed on the show, this version of Carnage was quite noticeably tamer and less violent than his appearances in the comics, merely draining life from his victims, which was later restored, rather than killing them outright. He was eventually defeated by Spider-Man, Iron Man, and Venom, as he, Venom and Dormammu were sent hurling through a dimensional vortex.

In the series finale, the Carnage symbiote emerged from a dimensional portal into an alternate reality, and bonded with the emotionally unstable Peter Parker of that world.

The joining of the two created the insane Spider-Carnage, who was threatening to destroy all of reality, using an invention that combined the dimensional warp generator and an enormous bomb. The real Peter Parker, assisted by several other Spider-Men from other universes, were able to convince Spider-Carnage to fight the symbiote off.

This was done by bringing in Peter's Uncle Ben from a reality where he was not murdered. Peter knew the symbiote was influencing Spider-Carnage to commit this genocide, since they were in fact the same person and Peter could never kill another human being. Uncle Ben was able to convince Spider-Carnage he could still be a hero as long as he had the courage to reject the symbiote. However, the Carnage symbiote was too powerfully bonded and despite Spider-Carnage's best efforts, he could not get rid of it, so he threw himself into a dangerously unstable warp hole where he was killed by vaporization.

Unlike the unpredictable-but-somewhat-sane Venom, Carnage is thoroughly insane, and revels in chaos and destruction, taking lives at whim and without discrimination. Venom, on the other hand, usually safeguards innocent lives, possibly as a side effect of bonding with the sane human Eddie Brock. Lifelike robotic copies of Carnage and Venom appear in "The Haunting of Mary Jane".

Chameleon was an international hitman and spy. He also cannot (or does not) speak while he is in his true form, though his grunts were once provided by Jim Cummings. Though in the episode "Framed" Richard Fisk indicates that Chameleon told them about Peter Parker's parents. Chameleon has a belt, which is capable of capturing an image of a person, so that he can turn into a person to disguise himself, and then makes a perfect imitation of his disguise's voice. In his first appearance, he attempts to kill two diplomats at a U.N. conference to start a war, but is foiled by Spider-Man who easily picks the Chameleon out from the crowd since he had taken the appearance of Peter Parker, secretly Spider-Man's alter ego. In "The Insidious Six" and "Battle of the Insidious Six", Chameleon became a member of the Insidious Six. In "Framed" and "The Man Without Fear", he was working for the Kingpin's son, Richard Fisk in framing Peter Parker for getting restricted government information. But Parker's name was cleared by the machinations of Spider-Man and Daredevil, thus Richard and the Chameleon were sentenced to jail. Later, in "The Cat", it was revealed that he was jailed in S.H.I.E.L.D. for his dangerous international activities and he was assigned by the Kingpin to release Felicia Hardy's father. Chameleon was infused with a techo-organic virus prior to his incarceration in S.H.I.E.L.D., which made him part-machine and was able to change into any person without the belt. He successfully disguised himself as Felicia's father so no one would know the real Hardesky was abducted. Eventually, his ruse was uncovered by Nick Fury. Later, in "Six Forgotten Warriors" parts 1-5, he was rescued by the rest of the Insidious Six and became a member again, but would betray them and join forces with his foster father, the Red Skull (they have no relation in the comics), and his brother, Rhienholdt Kragov, who would later become Electro. In the end, Electro and the Red Skull, along with Captain America, were trapped in a time dilation loophole and the Chameleon escaped, never to be seen again.

Susan Choi (voiced by Amy Hill) was a federal agent part of the organisation plotting to frame Peter Parker. She was working with Richard Fisk and the Chameleon, after Terri Lee discovered that this led to Cho, Fisk and the Chameleon being imprisoned.

Doctor Doom (voiced by Tom Kane) is an enemy of the Fantastic Four. The show re-imaged Doctor Doom's role in the Secret Wars. In this episode, Doom turned part of the alien world he was on into New Latveria after driving Doctor Octopus out of the territory. However, he did not use his ruling powers for evil and had the aliens who lived in his country live in peace and harmony. He even kidnapped the Thing only to cure him of his deformity and powers, turning him back to Ben Grimm. After tricking Grimm into revealing the knowledge provided by Spider-Man on how the Secret Wars began, Doom then stole the powers from the Beyonder and almost killed the superheroes that Ben fought aside with. But the Thing knocked Doom out and the powers of the Beyonder were returned to the mystic figure himself. Doom was then returned to Earth with no memory of these events, along with every other superhero and villain.

Dormammu (voiced by Ed Gilbert) first appears in the Spider-Man: The Animated Series episode Sins of the Fathers Chapter I: Doctor Strange. In the show, Dormammu is a demon from another dimension who must consume souls in order to sustain himself. In this episode, he orders Mordo to steal the Wand of Watoomb to free him so he can enter this dimension and feed on the souls of New Yorkers. Dormammu is stopped by the combined efforts of Spider-Man and Dr. Strange. Dormammu and Mordo appear again in the episodes "Venom Returns" and "Carnage". In these episodes, Dormammu orders Mordo to reunite Eddie Brock with his symbiote, releasing the threat of Venom once more. Mordo then orders Venom to retrieve a device from Stark Enterprises that can open portals from other dimensions, so that Mordo can bring Dormammu to this dimension. After seeing that Venom's hatred of Spider-Man is preventing him from retrieving the device, he orders Mordo to give the symbiote's recently born offspring to madman Cletus Kasady, which creates the composite villain Carnage. After retrieving the device, Carnage is tasked with collecting life energy for Dormammu in order for him to get enough strength to enter our world, which he does. However, in the end, Dormammu, Mordo, and Carnage are defeated by Spider-Man, Iron Man and Venom, and Spider-Man expels Dormammu back to his dimension. Carnage is pulled into Dormammu's dimension along with Venom, and with the device destroyed (thanks to Iron Man, who makes a guest appearance in this episode), Dormammu is stopped from entering our world again.

Electro (voiced by Philip Proctor) made an appearance in season five. In this show, he was Rheinholt Kragov, instead of Max Dillon. During the course of Six Forgotten Warriors in which he appears, it is revealed that this version of Electro is the Red Skull's son and the Chameleon's half-brother. Prior to his father's return, Rheinholt posed as the chief of police of a Russian city. While he posed as Red Skull, he was unmasked by Kingpin to not be the real Red Skull as Kingpin noted that the real Red Skull would've broken free from Kingpin's grasp. In this portrayal, Electro is much more powerful than his comic book counterpart, as he is seen taking over the circuitry of vast machines and essentially brings all of S.H.I.E.L.D. to its knees. He is only defeated when Spider-Man tricks him into connecting himself to a machine made to generate a void in the space-time continuum, which traps him inside a time loop. Electro appeared in most of the episode arc in the episodes, Unclaimed Legacy, The Secrets of the Six and The Six Fight Again before he became Electro and as the villain, he only appeared in The Price of Heroism.

NOTE: Electro appears so late and so different in the show because during the show's creation, a Spider-Man film helmed by James Cameron was in production. Electro was to be one of the film's villains, alongside the Sandman, who was the only major Spider-Man villain to never appear in the series. This is also why Spider-Man's origin story was not told until much later into the show.

The Kingpin (voiced by Roscoe Lee Browne) is the main antagonist of the series. This Kingpin is fairly close to the comics version, but he is often occupied with manipulating super-powered characters to do his bidding. He's the mastermind behind the creation of the Spider-Slayers and is responsible for the creation of the Insidious Six, the animated equivalent of the Sinister Six. He's also involved in a power struggle with crime boss Silvermane. Initially he operated behind the scenes until a two-part episode where his identity was revealed to Spider-Man with the help of Daredevil, who was seeking revenge for his father's murder at Kingpin's hands. The Kingpin's associates here was first Alistair Smythe but after Smythe almost betrayed the Kingpin because he believed Kingpin was going to kill him for his son being in jail in the two-part episode where his identity was revealed to Spider-Man, Dr. Herbert Landon replaced him. This series also described an origin for the Kingpin, somewhat different from the comic-book version. As a boy, Wilson Fisk (born Wilson Moriarty) was influenced by his father, who sought employment as a mob criminal. When older, Fisk assisted his father in robbing banks and jewelry stores, culminating in one robbery where his father escaped but Fisk, hampered by his already-considerable weight, was captured by the police. In adulthood, after being released from prison, Fisk emulated his father's goal and climbed to a position of seniority within the mob, adopting the alias "Kingpin". He has had his police file destroyed, removing all record of his earlier arrest and imprisonment. He arranged for the arrest and conviction of his father, still an aspiring but minor criminal, and declared that he did this because "Sacrifices must be made". He maintains a strained relationship with his wife Vanessa, who is well aware of his activities, while his son Richard works for him. By the series finale, in an alternate dimension, the Kingpin was working with the evil Spider-Carnage, the Green Goblin, the Hobgoblin and the cyborg version of Alistair Smythe to build a mind-controlling device while Spider-Carnage's real intentions were to destroy all of reality. The Kingpin realized this from the primary Spider-Man of the series. After Spider-Carnage's plans failed and he traveled to another reality to continue his plans, his building exploded but the mystical Madame Web saved the three Spider-Men and the Kingpin from the explosion and sent the Kingpin behind bars. In the alternate dimension Spider-Carnage traveled through to continue his plans, he teamed up with the corrupt lawyer of that dimension's Spider-Man, who was Fisk, to rule all of reality, but nevertheless, Spider-Carnage admits his real plans when he kidnapped the alternate dimensional Spider-Man of that dimension's fiancée, Gwen Stacy. Kingpin bearhugs Spider-Man twice in the series, first time in "The Spot" and then again in "Goblin War". The Kingpin states that about two percent of his body mass is fat, and that he is 350 pounds of muscle.

Notably, the Kingpin has many enemies, other than Spider-Man, his most recurring enemy. Among these foes of his include opposing crime boss Silvermane, and the two supervillains, the Green Goblin and the Hobgoblin. Also unlike the comic book version mostly every supervillain works for him, or he forces them to work for him, like he did to Doctor Octopus in season 4. He was the most powerful criminal that Spider-Man faced.

There were two alternate versions of Kingpin that appeared in the final two episodes.

• The first version was from Ben Reilly's dimension in which Spider-Carnage had him use Hobgoblin and Green Goblin to destroy much of the city. After the explosion that would've destroyed the Multiverse was thwarted, Madame Web teleported that Kingpin to a prison.
• The second version was a lawyer to the high-tech Spider-Man where he was tricked by Spider-Carnage into helping him.

Norman Osborn (voiced by Neil Ross) was indebted to the Kingpin for various shady loans that Osborn was unable to repay, so the Kingpin asked Osborn to kill Spider-Man to repay the loans. Osborn then hires Spencer Smythe to build Spider-Slayers to kill Spider-Man. After the plan fails, Osborn hires the Hobgoblin to kill Wilson Fisk. But the assassination is foiled by Peter Parker, and the Kingpin suspects a conspiracy against him. For his failure, Osborn fired the Hobgoblin. Hobgoblin then joins with Kingpin and kidnaps Norman's son, Harry Osborn, for the crime boss. When Hobgoblin sees that Kingpin will not pay him immediately, he eventually betrays the Kingpin by going to Norman for better weapons to kill the Kingpin and Osborn can have his company and son. Osborn gives him a better glider and weapons and by the time the Hobgoblin returned to the Kingpin, Hobgoblin realizes that the Kingpin knows he was going to double-cross him, causes the Kingpin to flee, having the Hobgoblin think he died and took over his empire. The story concludes with the Kingpin, Osborn, and Spider-Man all loosely allying themselves to defeat the Hobgoblin, who escapes after crash-landing into the water. In the end, Osborn sells 50% of his company to the Kingpin in order to repay his debt, Harry Osborn is returned safely, and the Kingpin rebuilds his headquarters. Later on, in the two-part second-season finale, Osborn refuses to grant loans to Adrian Toomes's projects because Toomes was too old and senile. Therefore, Toomes became the villain known as the Vulture in order to try to kill Osborn, but as usual, Spider-Man saves Osborn and defeats the Vulture.

Later in the episode, Enter the Green Goblin, Norman Osborn and his partner, Mendel Stromm, were coerced into creating a formula for the Kingpin that grants superhuman strength. An unstable reaction resulted during one experiment and Norman disappeared in the explosion, presumed dead. His son Harry blamed the Oscorp stockholders J. Jonah Jameson, Anastasia Hardy, and Wilson Fisk (the Kingpin) among others for the death of his father.

Soon a mysterious airborne figure, identifying himself as the Green Goblin, began kidnapping the stockholders one by one. Spider-Man uncovered an underwater base where the Goblin intended to kill everyone he had kidnapped. Fighting the Goblin, Spider-Man unmasked him, only to discover that the Green Goblin was Norman Osborn, rather than the suspected identity of his son, Harry. Amnesia ensued and Norman was unable to remember his dual identity. The following morning at OsCorp, he announced that he will no longer build chemical weapons, much to the Kingpin's secret dismay. Osborn appeared as himself again in the episode The Ultimate Slayer at an engagement party for Harry, who was going to marry Mary Jane Watson. However, the party was crashed by Alistair Smythe, recreated as a cyborg under the Kingpin's machinations, and Smythe kidnapped Norman, his son and Mary Jane, bringing them back to the Kingpin's lair. There, the Kingpin reveals to Osborn that Spencer Smythe did not die in the explosion back in The Spider Slayer, but that he escaped early on before the explosion ensued at OsCorp and was met up by the Kingpin. But rather than killing him outright for his failure with the Spider Slayer's goal to kill Spider-Man, the Kingpin sees that he may still have some future use for Spencer, so he places him in chronological suspension. Spider-Man overhears this and tells this to Alistair, who turns on the Kingpin completely, destroys his lair and takes his chronologically suspended father away with him, thus ending whatever relationship the Kingpin and Alistair had between each other. Spider-Man managed to have saved Norman, Harry and Mary Jane, but unfortunately for Harry, Mary Jane decided to call off her engagement with Harry to be with Peter Parker.

In Goblin War!, Norman Osborn sees the Green Goblin in all reflections and becomes him again after being threatened by the Kingpin that if he did not reveal the Hobgoblin's identity, who the crime boss was currently in league with, then Harry will suffer the consequences. In this episode, he and the Hobgoblin clash over Dr. Ohn's Time Dilation Accelerator, which was what the Hobgoblin possessed that lured the Kingpin into an alliance with him. Near the end, the Green Goblin tracks the Hobgoblin down to Jason Phillip Macandale's home, since the Goblin knew from prior knowledge as Norman Osborn that Macandale was actually the Hobgoblin. So he kidnaps Macandale and his fiancé, Felicia Hardy, with the Time Dilation Accelerator, takes them to OsCorp and ties them up over a chemical vat to slowly lower them there to their doom. But Spider-Man arrives just in time for a brief fight with the Green Goblin, saves Macandale and Felicia and nearly destroys the Time Dilation Accelerator with one of the Goblin's weapons. But, seeing that there is still some power left in the Accelerator, the Goblin takes his chances into a portal, stating to Spider-Man beforehand that he'd rather be trapped in limbo than be defeated again by the superhero. However, at the end of the episode, the Goblin manages to make it back into his own dimension because the Accelerator had more power than either him or Spider-Man thought, and he starts to modify the machine while plotting the downfall of his enemies like Spider-Man and the Kingpin (the Hobgoblin is now jailed at this point because of his identity of Jason Macandale now revealed). In the next episode Turning Point, the Green Goblin finds out Spider-Man's secret identity with the Time Dilation Accelerator. As Osborn, he nearly reveals Peter's identity as the superhero in front of everyone at Harry's birthday party, but when Peter catches on, he causes a fire that abruptly interrupts Osborn. Next, Spider-Man and the Green Goblin's ensuing fight goes from Osborn's home atop the George Washington Bridge. But at the Bridge, the Green Goblin uses the Accelerator again, implying that he will go to Peter's Aunt May's home to kill her. But by the time Spider-Man arrives, the Goblin appears and tells him that he decided to switch targets, implying that he is intending to kidnap Mary Jane. The Goblin succeeds in this task and takes her back to the George Washington Bridge, where he and Spider-Man have their final confrontation. And during the battle, both Mary Jane and the Time Dilation Accelerator fall over a ledge, and the machine is accidentally activated, creating a portal which MJ falls into. Neither Spider-Man or the Goblin know what happened, which makes Spider-Man assume that MJ possibly died and vows vengeance on the Green Goblin. The villain tries to escape using the Accelerator again, but finds that it is almost out of power. Nevertheless, he manages to reactivate it before Spider-Man could reach him, but accidentally creates an unstable portal that sucks anything in its path inside. Spider-Man initially tries to push the Goblin in, but he then realizes that vengeance will not bring Mary Jane back, so he tries to save the Goblin. But the villain activates his glider to attack Spider-Man from behind to push both of them into limbo. However, Spider-Man's spider sense tingles, warning him of the glider coming in, so he dodges it, and the glider hits the Green Goblin in the chest where the Time Dilation Accelerator is situated, thus destroying it and pushing the Goblin into limbo all by himself, where the unstable portal finally ceases to be.

Though this is the last time Spider-Man and the Green Goblin would clash, this is not the end of Norman Osborn's saga. In the episode The Return of the Green Goblin, Norman, as the Goblin, contacts Harry through a spiritual link, saying that if Harry becomes the Green Goblin and kills Spider-Man, then he will see his father again. Harry hesitantly complies and, figuring out thanks to Norman that Spider-Man is Peter Parker, the new Green Goblin tries many times to kill Spider-Man while having the Punisher track them both down. Harry goes back to OsCorp for better weapons against Spider-Man and the Punisher, but Spider-Man arrives and tells Harry that his father is the Green Goblin, which the latter confirms, to Harry's shock. But Harry realizes that this is his family's legacy to be the Green Goblin, so he confronts Spider-Man and the Punisher again at the George Washington Bridge. Spider-Man defeats Harry by tackling him into the river and destroying his glider, and Harry's unconscious form is taken by the Punisher back to Peter's house for interrogation as to Mary Jane's whereabouts. Later, after the clone of Mary Jane arrives to be mistaken by everyone to be the real MJ, Spider-Man takes Harry to Ravencroft Institute to cure him of his insanity as the Green Goblin and make him think his knowledge of Peter being Spider-Man as false. But in the fifth-season premiere, The Wedding, when Harry hears that Peter and Mary Jane's clone are getting married, Norman contacts Harry again, inspiring him to escape Ravencroft and team up with Alistair Smythe, now under the employ of the Kingpin's enemy, Silvermane, to create Goblin Warriors, robot battle droids under Harry's command. He decides to use them at Peter and MJ's clone's wedding to kill Spider-Man so that Harry could marry MJ's clone, but the Goblin Warriors fail to kill Spider-Man, thanks to the help of the Black Cat and Smythe's Mega Slayer robot controlled by the Kingpin, and Mary Jane's friend Liz Allen tells Harry that she loves him. This makes Harry think that he's insane again, goes back from his plan on trying to marry MJ's clone and goes back to Ravencroft to be cured of his insanity for good.

Norman Osborn appears again as Green Goblin in I Really, Really Hate Clones, the first part of two-part series finale, in which he was working for Spider-Carnage and the Kingpin in an alternate reality alongside Hobgoblin. This is simply a parallel version of the character in this other universe, as there was nothing that implied of the previous Norman Osborn. When the Green Goblin was confronting the Spider-Men from different realities who had joined forces to stop Spider-Carnage, all of them used their webbing to stick him to a floor of the Kingpin's tower. In the next episode, although he did not appear in it, the Green Goblin dies in an explosion that destroys the tower.

Harry is voiced by Gary Imhoff. In this adaptation, Harry was Peter Parker's roommate in college. Their friendship seemed less strained and Gwen Stacy was not featured. Harry played an overall minor role, though he did act as a something of a rival for Mary Jane Watson's affections in at least one episode.

After the original Green Goblin becomes trapped in the Negative Zone, he appears to Harry. Soon, Harry unwilling made into a replacement for and by the original Green Goblin, who contacted him while in a limo. Harry tried to kill Spider-Man numerous times while the Green Goblin promised to allow Harry to see his presumed deceased father, Norman Osborn. However, Harry always failed, once getting into some trouble with the Punisher as well. In the end, Harry realized in horror that the Green Goblin was his father and instead of wanting to quit, he began to lose his sanity. Now acting as if he enjoys becoming the new villain, he continued to want Spider-Man dead. This led to a fierce confrontation where Spider-Man knocked him out while on the George Washington Bridge. He was then taken to Ravencroft by Spider-Man.

Later, when Peter Parker and the clone of Mary Jane Watson were getting married, Harry found out and grew insane, as he was in love with MJ. He interrupted the wedding and threatened to blow up the church the wedding was taking place, wanting Mary Jane to marry him instead of Peter. In the end, Liz Allan appealed to Harry, convincing him not to blow up the church nor marry Mary Jane's clone. Told by Liz that he is loved, he walked out peacefully and the wedding resumed.

Hammerhead (voiced by Nicky Blair) has a head that is made of an unbreakable steel known as adamantium, which is what Wolverine of the X-Men's skeleton is covered with. Hammerhead worked for the crime lord known as Silvermane and was with him when some of the other crime lords were plotting to overthrow the Kingpin. When Silvermane sent Hammerhead and his men to steal the mystic Tablet of Time, which can reduce the user's age, when it was arriving in New York, Spider-Man foiled the attempt and the object was taken to Empire State University to be observed by Dr. Curt Connors. As for Hammerhead, Silvermane harassed Hammerhead for his failure and was thinking about replacing him with someone else. Hammerhead quit working for Silvermane and began to work for the Kingpin. When Alistair Smythe used his Mega Slayer robot to steal the Tablet of Time, Silvermane decided to blackmail the Kingpin after using the villain known as Tombstone to kidnap the crime lord's wife and Dr. Connors who knows how to operate the Tablet. Kingpin got back to Silvermane by sending Hammerhead to kidnap his daughter, Alicia, and a deal was made that if Kingpin give away the Tablet and Alicia to Silvermane, then his wife, Vanessa, would come back to him completely safe and sound. Kingpin refused to give the Tablet and Alicia away by the meeting where a brawl involving Spider-Man occurred and Silvermane had taken the Tablet, Dr. Connors and his wife, Margaret, while Vanessa came back to Kingpin unharmed. Hammerhead tried to kidnap Alicia, but Spider-Man stopped him and defeated Hammerhead, where Alicia subsequently took Spider-Man away while Hammerhead was out. After Spider-Man saved Dr. Connors and his wife, Silvermane was turned to a baby from overuse from the Tablet and though the mystical object was thought to have been destroyed in an explosion, Hammerhead had saved it and brought it back to the Kingpin so the crime lord could sell it for a large price. But the crime lord saw it caused Vanessa to divorce him and afterward, Kingpin demanded Hammerhead would get rid of the Tablet. And Hammerhead did by selling it to the villain known as the Vulture.

Herbert Landon (voiced by David Warner) was a former friend of Hank McCoy. He first appeared in season two as a scientist working for the Brand Corporation, but secretly working for the Kingpin. He despised mutants and had developed a chemical that would destroy them, while he pretended to be making a mutant army for the Kingpin and lied to the public he was working on a mutant cure. When the Hobgoblin attempted to steal Landon's formula, a fight ensued in which Landon was doused in his own chemicals, turning him into a 30 ft (9.1 m) mutant, needing to feed on electrical energy to survive to compensate for the energy that his own cells were no longer producing after being exposed to the mutant 'cure' while lacking any mutant cells for it to destroy. Luckily, the X-Men and Spider-Man were able to reverse the procedure, but the accident left Landon permanently mutated down the right side of his body (resembling Two-Face). After that, he joined forces with Spider-Man's arch foe, the Kingpin, and served as his key scientist.

Following this, Landon was portrayed as half-human and half-reptilian (his mutant form), and Kingpin's sidekick. He used his knowledge of bioengineering to transform Alistair Smythe into the Ultimate Spider-Slayer and became replacement partner to the Kingpin, taking Alistair's spot. He also recreates the Captain America super-soldier formula in order to turn Felicia Hardy into the Black Cat. In The Awakening, he enlists the services of the Shocker to help him steal the Neogenic Recombinator and at the end of the episode, Landon is seen in the hospital right after Morbius drains his plasma. Landon makes a cameo appearance in the following episode, where in he was hypnotized by Mirium into stealing the Neogenic Recombinator from Kingpin. He was later seen fully recovered in the fifth-season premiere and last appeared in the five-part episode saga that followed the fifth-season premiere.

Herbert Landon did not possess any superhuman powers (except in his first monstrous mutant form), but was a brilliant scientist, and an expert in genetics.

The Hobgoblin (voiced by Mark Hamill) is a criminal-turned-assassin who, in his first appearance, gets hired by Norman Osborn to kill New York's most feared criminal mastermind, Wilson Fisk, also known as the Kingpin, in which the Hobgoblin himself did not know that Fisk was the Kingpin. Osborn was indebted to the Kingpin for various shady loans that Osborn was unable to repay. After the assassination is foiled by the appearance of Spider-Man and Peter Parker when they save Fisk when he was going to create a criminology school and cause the Hobgoblin to flee, the Hobgoblin demands Osborn to pay him and he will take another chance to kill Fisk. But Osborn refuses to pay him and fires him. This sets off a chain of events in which the Hobgoblin is curious about why Osborn wants Fisk dead and while snooping around Fisk's building, he is captured and realizes Fisk is the Kingpin.

Just when the Kingpin would kill the Hobgoblin, the Hobgoblin asks for a chance for a partnership with the Kingpin and defeat Norman Osborn, the man who hired the Hobgoblin to kill Fisk. Kingpin was so despised at hearing this news and Osborn for what he did that he aligns with the Hobgoblin and sends him to kidnap Harry Osborn, Norman's son, in order for the elder Osborn to make good on his payment. But when Hobgoblin sees that Kingpin will not pay him immediately, he eventually betrays the Kingpin by going to Norman for better weapons to kill the Kingpin and so that Osborn can have his company and son.

Osborn gives him a better glider and weapons, and gets into a prolonged battle with Spider-Man across New York, ending with the Hobgoblin presuming that Spider-Man died in an explosion. Later, by the time the Hobgoblin returned to the Kingpin, Hobgoblin realizes the Kingpin knows he was going to double-cross him, causes the Kingpin to flee, having the Hobgoblin think he died and took over his empire. The story concludes with the Kingpin, Osborn, and Spider-Man all loosely allying themselves to defeat the Hobgoblin, where Spider-Man fights the Hobgoblin in the Kingpin's lair and nearly destroys it. The superhero saves Harry, bringing him back to Norman and expelling the Hobgoblin from Fisk's empire, having the crime lord reclaim it as his own. The Hobgoblin tries one more time by trying to kill Osborn and his son, but Spider-Man saves them and has the Hobgoblin crash into a nearby river. In the end, Osborn sells 50% of his company to the Kingpin in order to repay his debt, Harry Osborn is returned safely, and the Kingpin rebuilds his headquarters.

Hobgoblin went on to make two more appearances, starting with the crossover of X-Men: The Animated Series. He discovered that Dr. Herbert Landon, who was said to cure mutation, (and secretly promising to make a mutant army for the Kingpin) was planning to exterminate all mutantkind. The Hobgoblin blackmailed Landon that if Landon did not give him the money he thought he deserved, then Landon's real intentions will be revealed to the public. However, Spider-Man tracked the Hobgoblin down and learned that Landon could potentially make good on his threat. Teaming up with Wolverine, Spider-Man tracked Landon to his lab. They battled Hobgoblin, who managed to escape with discs outlining Landon's master plan. Spider-Man and the X-Men managed to defeat an ironically mutated Landon after the Hobgoblin escaped, and Landon was taken into a hospital to be cured of his mutation.

The Hobgoblin's next appearance was in the second Green Goblin story arc, in which his identity was revealed to be Jason Philip Macendale, fiancé to Felicia Hardy and had a portal-creating machine called the Time Dilation Accelerator. Once his identity was revealed to his fiancé, he and Felicia were kidnapped by the Green Goblin who stole the Accelerator from the villain and took both fiancé to their doom at OsCorp where they will be killed in an acid pool. Spider-Man forced the Goblin into limbo (right before he would return in the next episode), saved Macandale and Felicia and their relationship ended. The Hobgoblin was then immediately taken into custody afterward.

An alternate-reality version of the Hobgoblin appeared in Part One in the series finale of Spider-Man as an enemy of the Scarlet Spider. He battled the various Spider-Men from different realities to try to stop Spider-Carnage from destroying all of reality, suffered defeat, and was tied up. In the last episode of the series, the Kingpin's building (where the beaten Hobgoblin was kept) exploded. Madame Web used her powers to save Spider-Man, his associates, and the Kingpin, but Hobgoblin perished in the explosion.

An interesting fact is that in his first appearance his skin was not white, but black. Also, his hair was a reddish color rather than black like he does in the comics.

Hydro-Man (voiced by Rob Paulsen) was portrayed as Mary Jane Watson's former boyfriend. In an entire episode, Hydro-Man stalked Mary Jane and Spider-Man tried to stop him, but Hydro-Man was too powerful in battle as he can create and control water. At the end of the episode, Spider-Man and Mary Jane worked together to defeat Hydro-Man. They led him away from any water source, weakening him so much that when he tried to attack Spider-Man, he collapsed and evaporated.

In "The Return of Hydro-Man", it is revealed that Dr. Miles Warren created a clone of Hydro-Man, using what was left of the original Hydro-Man from the ground he was killed on. However, the clone was still in love with Mary Jane so it demanded that Warren make a clone of Mary Jane as well. Even though it was hard to clone Mary Jane (she fell into a dimensional portal in Episode #41), Warren succeeded but Mary Jane's clone decided to be with Peter Parker rather than Hydro-Man. When it escaped, Hydro-Man later attacked them at Niagara Falls on their honeymoon. When all that was revealed by Warren himself, the scientist discovered that the clones were not stable and would vaporize. The clone of Hydro-Man went first and then MJ's clone.

When Spider-Man was bitten by the radioactive spider that gave him his powers, it also had a terrible side effects of giving him a disease that was part of his evolutionary cycle. The cycle would eventually lead Spider-Man into becoming the sadistic beast known as the Man-Spider (vocal effects provided by Jim Cummings). When Man-Spider was terrorizing citizens in New York, the crime-killing vigilante known as the Punisher and one of Spider-Man's past enemies who was also one of his friends, Sergei Kravinoff, teamed up and temporarily cured Spider-Man of his disease. How Spider-Man permanently got rid of the disease was when he was fighting the villain known as the Vulture in the two-part second-season finale. One of the Vulture's powers was absorbing youth from people younger than he was and when Vulture absorbed Spider-Man's youth, he also absorbed his powers but consequently, he even absorbed his disease. Vulture demanded from Dr. Curt Connors, who was treating Spider-Man's disease at the time, to help cure him while at the same time teaming up with the Scorpion to defeat Spider-Man after draining the disease back into Spider-Man. However, Connors betrayed Vulture and only gave Spider-Man back his youth and powers, but left his disease back in the Vulture. Man-Spider was never seen again, with the exception of the two-part series finale of the show where one of the alternate dimensional Spider-Men was mutated with only six arms, as that was part of Spider-Man's evolutionary cycle, but he became the Man-Spider. Fortunately, that Man-Spider was transported by the mystical figure known as the Beyonder into a different reality with him.

Man-Spider is similar to Man-Bat from Batman by the character's opposite names like the regular Batman and Spider-Man's names. But Spider-Man and Batman are human and Man-Spider and Man-Bat are mutated forms.

Miranda Wilson (voiced by Beverly Garland) was an actress who was nearly killed in an accident involving her movie. She had fallen into the river. When she reached the shore, she saw in the river's reflection that half her face was badly scarred. She went through the catacombs hiding her face. Mysterio found her collapsed on the ground and rebuilt her by making her part cyborg. When she learned that they remaking her movie, she kidnapped Mary Jane (who looked a lot like her) and tried to put her mind into Mary Jane's body but was stopped by Spider-Man and Mysterio. She and Mysterio were killed in an explosion after revealing their love for each other.

In this show, Mirium (voiced by Nichelle Nichols) is Blade's Mother. She was turned into a Vampire by Blade's father. She came to New York in order to turn Spider-Man, Detective Terri Lee, Black Cat, and Blade, along with many other people into vampires.

Mysterio (voiced by Gregg Berger) was a supervillain who blames Spider-Man for ruining his reputation. In his first appearance, Mysterio frames Spider-Man for various crimes, but his plan is exposed by Spider-Man and Detective Terri Lee, and he is jailed. Later, he becomes a member of the Insidious Six in the episodes "The Insidious Six" and "Battle of the Insidious Six", but the entire team fails and disbands to avoid being arrested. In Mysterio's final appearance in the series, "The Haunting of Mary Jane", his ex, Miranda Wilson, creates a studio in secret and kidnaps the clone of Mary Jane Watson and Spider-Man teams up with Mysterio, battling robot versions of villains Spider-Man fought in the past in a deathtrap Mysterio had designed to one day lure Spider-Man to and kill him. Spider-Man discovers that Mysterio was in love with a woman named Miranda Wilson, a former actress who was disfigured and planned the entire kidnapping to swap bodies with the similar-looking Mary Jane. The studio exploded, Spider-Man saved the Mary Jane, and Mysterio dies in the explosion, staying with Miranda to the end. In the five-part "Six Forgotten Warriors" storyline, in which the Kingpin hires the Insidious Six again, the Vulture replaces the late Mysterio.

Doctor Octopus (voiced by Efrem Zimbalist Jr.) made several appearances in the show. Dr. Octopus used to be Otto Octavius, who was Peter Parker's science teacher at Science Camp. This relationship of former mentor and pupil made Dr Octopus fond of Peter even after his descent into villainy. One day, Octavius created a fusion experiment, using four metal tentacles. When the experiment exploded, Octavius' tentacles were permanently stuck on his back. In his first appearance, Dr. Octopus kidnaps Felicia Hardy and J. Jonah Jameson for ransom from Felicia's mother, as Felicia's mom did not have patience to fund Ock's experiments before he was a villain. When Peter calls him, Dr. Octopus decides to have Peter bring the ransom. But Ock reveals he would not release Felicia and Jameson anyway but after he was defeated by Spider-Man, who Peter was secretly, after Ock threw Peter off into another part of the abandoned space lab Ock hid out in, Dr. Octopus was captured, and was taken to jail.

Later, he became a member of The Insidious Six in the first two episodes of Season 2 and the five-part episode, "Six Forgotten Warriors" in Season 5, and one of the Kingpin's enforcers after the first episodes of Season 2, helping him commit crimes. He even came far as unmasking Spider-Man with the Insidious Six but since Spider-Man did not fight as good as he usually does because it was part of Spider-Man's lively evolution, Ock thought he was a fake. He also came to wiping out Spider-Man's memory and making him think the two were partners-in-crime. But thanks to Spider-Man's fangirl, Tiana, and a cabbie named Mousie, he got his memory back and Octavius was sent back to jail.

Ock eventually became aware that Anastasia Hardy's husband, John Hardesky, was a cat burglar known as the Cat and used this information to blackmail Mrs. Hardy into giving him his money. But he was abducted by the Kingpin and was forced to work for him and his plans were not to reveal to the world that Mrs. Hardy's husband was a crook but merely use John Hardesky because he was jailed for knowing the super-soldier formula that created Captain America during World War II.

After Hardesky was kidnapped, Ock was sent to kidnap Felicia to blackmail Hardesky that if he did not reveal the super-soldier formula, she will die. Hardesky revealed the formula and it was tested on Felicia, turning her into the Black Cat. His final appearance in the show was in the three-part "Secret Wars", in which he ruled an alien planet city renamed "Octavia".

Unfortunately, it was stolen by Dr. Doom and renamed it New Latveria, forcing Ock to work for the Red Skull. When Doom absorbed the Beyonder's powers, Doom transported Alistair Smythe, the Red Skull and Ock back to Earth with them getting amnesia from the events. In the series finale of this show, despite Ock not appearing in it, Spider-Man was sent by Madame Web and the Beyonder to stop the evil Spider-Carnage from destroying all reality, and helping Spider-Man were other Spider-Men from different realities. One of them had metallic tentacles like Ock and that Spider-Man explained it was a "souvenir from my last fight with Doc Ock". It is unknown if that Spider-Man killed or had Ock killed in his reality, even by accident, and stole his robotic arms, but it seems likely.

His only appearance was in the second-season premiere of the series, "The Insidious Six", where he is portrayed to be one of the crime lords with Silvermane and against the Kingpin. He did not have any spoken dialogue in this scene.

Red Skull (voiced by Earl Boen) made a cameo in the second episode of Season 4 in the episode "The Cat". Later on, during the Six Forgotten Warriors story arc in Season 5, it was explained that after Red Skull and Captain America fought, they were trapped in a vortex. 50 years later, his son, Rhineholt, frees them both from the vortex, only for them to be trapped again. Finally, in Secret Wars, the Skull was sent by Beyonder to an alien planet to fight Spider-Man and his team of superheroes, but was sent back where he came from with no memory of the events when Dr. Doom absorbed the powers of the Beyonder.

Richard Fisk (voiced by Nick Jameson) is loyal to his father and heads a company front called Fisktronics. In the two-part episode of Framed and The Man Without Fear, Richard frames Peter Parker for selling government secrets, with the help of the Chameleon. Spider-Man teams up with Daredevil in an attempt to clear his name. They capture Richard, and he is sent to prison for his figured crimes and after Peter was cleared of all charges.

Later on, Richard teams up with Tombstone in Ryker's Island Prison and agrees to frame Joseph Robbie Robertson in the episode Guilty. They have him kidnapped, put a gun in his hand while he was unconscious and was sent to trial for a crime he did not mean to commit. He was originally supposed to go to New York State Penn. but with the help of Richard's father, the Kingpin, Robbie was reassigned to Ryker's Island where he would be there for the next fifteen years. But Spider-Man apparently figured out about Richard and Tombstone's plan and busted into Ryker's Island to save Robbie. And just when Richard and Tombstone would take off with their valuable cellmate in a helicopter, Spider-Man accidentally has the copter crash but not before saving Robbie (and the pilot, who was working for the Kingpin under Richard and Tombstone's interests) and have Richard and Tombstone fall back to Ryker's Island ironically.

Richard made his last appearance in the show in the Season 4 finale, The Prowler, in a flashback appearance when he was saved by Hobbie Brown from getting killed by bags of powder from another incarcerated criminal. Richard told his father of the news and got Hobbie out of prison.

Little is known about the past of Rhino (voiced by Don Stark). He first appeared as hired muscle for The Kingpin, and was ordered to steal the Promethean X from John Jameson's shuttle. After Spider-Man nearly killed him in his black symbiote costume, Rhino left their battle petrified of the wall-crawler.

What he lacks in intelligence, he makes up for with brute strength and a huge horn on the top of his head. Rhino is loyal to the Kingpin, and refused to reveal any information about his employer to Spider-Man, and chose to suffer prison rather than squeal on his boss. This loyalty was rewarded when Kingpin offered the Rhino a part in his Insidious Six, a team of supervillains he created for the sole purpose of killing Spider-Man.

Scorpion (voiced by Martin Landau in seasons 1-2, Richard Moll in seasons 4-5) works for J. Jonah Jameson as an investigator, but is nervous, plump, and balding. In an attempt to discover Spider-Man's real identity, Jameson hires a scientist to use Dr. Connors' Neogenic Recombinator to turn Gargan into a superhero called the Scorpion. This incarnation is similar to the original comics version of the Scorpion, with superhuman strength and agility resulting from his genetic enhancement, using the Neogenic Recombinator, with scorpion DNA -referred to by the scientist who carries out the experiment as "a natural predator of the spider" - and an externally mounted cybernetic weapon-tail. He encounters Spider-Man on a roof top, and after a short battle is able to defeat the wall crawler. However, when he is about to unmask Spider-Man, his body is overcome with pain, and he begins to mutate further, developing yellow eyes, green skin, and talons on his fingers. At that point, madness at the horror of what he has become descend upon him. The Scorpion then attempts to gain access to a nuclear reactor, insanely believing it will restore him to normal ("Radiation made me this way, and I'm going to use it to turn me back!"). He bearhugs, and almost crushes Spider-man to death, until J. Jonah Jameson attacks Scorpion "because you're a greater evil". Spider-Man stops him, sending him to jail. In later episodes however he appears without the later mutations (although, sometimes when he gets really angry and attacks Spiderman, he gets his green face with fangs and yellow eyes again in a few seconds).

In "The Insidious Six", Scorpion becomes a member of the Insidious Six, created by the Kingpin, to kill Spider-Man, so Kingpin can settle off his debt with his enemy, Silvermane, but Spider-Man survived in the end. In "The Final Nightmare", he makes a plan to find a cure for his mutation by kidnapping Dr. Stillwell, the scientist who is responsible for turning him into a freak. Unfortunately, Stillwell destroys the Neogenic Recombinator in order to prevent any other beings like the Scorpion from existing again. Later, he meets Adrian Toomes, a.k.a. the Vulture, who is as smart as Stillwell, and when Toomes' lab was blowing up, Scorpion saved him. In "Partners", where Moll replaced Landau, he makes another attempt to cure himself, but fails when Spider-Man stops him (this attempt was a plot created by Spider-Man because he needed Scorpion to give to Alistair Smythe to save the Black Cat). In "The Wedding", Scorpion makes a bank robbery and Spider-Man is defeated. Then, he takes the wedding rings, so that he and his girlfriend Sara can get married. And finally, the Scorpion appears in the Six Forgotten Warriors five-parter as his last appearance as a member of the Insidious Six.

Shocker (voiced by Jim Cummings) is a villain for hire. Alistair Smythe created the wristbands and electro suit that gives Shocker his power. Like the Rhino, the Shocker is loyal to the Kingpin, and served as muscle for him, accomplishing whatever was asked of him. He was originally hired to retrieve potentially incriminating evidence about the Kingpin from Eddie Brock. He was almost defeated by Spider-Man and his alien costume when they fought in a belltower.

He later joined The Insidious Six and again worked for The Kingpin to try to kill Spider-Man. As one of Fisk's most loyal supervillains, he was called upon time and time again.

Herbert Landon enlisted the services of the Shocker to kidnap Morbius and use his DNA to cure his mutation and make himself and the Kingpin immortal. However, they also attempted to kill Felicia Hardy/the Black Cat, enraging Morbius, and are nearly killed.

Silvermane (voiced by Jeff Corey as an old man, Townsend Coleman as a young adult, Matthew McCurley as a child, and Cannon Young as a baby) is an elderly crime lord. Although he is a villain, he is not a supervillain, as he has no superhuman powers. He is, however, extremely intelligent. Although he is an enemy of Spider-Man, he does not see eye-to-eye with the superhero's other foes. He is a hated rival of Wilson Fisk (Kingpin), another crime lord. Silvermane is also the father of Alisha, aka Alisha Silvers, a name she went by in order to spy on the research of Dr. Curt Connors. He first appeared in the Insidious Six arc, where in he has Hammerhead hired to capture Spider-Man. In the end of Battle of the Insidious Six, he was rescued by Spider-Man from the Kingpin who tried to kidnap him.

Silvermane never enjoyed a normal childhood, as he grew into old age, he became obsessed with finding the secrets to youth and immortality. He heard legends of an ancient tablet which allegedly was inscribed with the formula to rejuvenate an individual's youth, the Tablet of Time. When the Tablet was unearthed and brought to the United States, he arranged for Tombstone to steal the tablet and kidnap Dr. Curt Connors (Lizard), who was studying its powers. Even with the intervention of Spider-Man, Silvermane was able to kidnap Connors' wife and force Connors to activate the tablet. In the comic book the formula is for a potion. Connors prepares the potion for him, while the lives of Connors' family were used as a motivator for him to successfully decipher the tablet. Spider-Man saved Connors but Silvermane took the potion anyway despite Connors warnings of it being unstable. In the animated series version of the story, Silvermane was transformed into a baby instead of being mildly rejuvenated. There, the tablet focuses the sun's rays into lasers which produce a greenish glow. Silvermane is initially turned into a young man, fights the Lizard then transforms further into a baby. The Lizard is bombarded by the rays and mutates back to human form. Dr. Connors concludes that they might by able to use the tablet to stabilize Spider-Man's mutations, but the tablet is stolen by Hammerhead, now working for Wilson Fisk (Kingpin) after Hammerhead quit Silvermane during an initial attempt at trying to steal the Tablet, foiled by Spider-Man. Fisk, dismayed that his wife left him, orders Hammerhead to get rid of the tablet. Hammerhead sells the tablet to an elderly man, presumably The Vulture, who uses the Tablet to temporarily restore youth to himself. The Vulture is unable to remain youthful for an expended period of time, transforming back and forth uncontrollably.

In a later episode, "Baby" Silvermane was seen to have retained his adult intellect. He and Alisha got the cyborg Alistair Smythe to kidnap the Black Cat (Felicia Hardy), blackmailing Spider-Man to apprehend the Scorpion. Baby Silvermane believes that he can use the Scorpion, who had been created through the use of Neogenics, to combine his genetic structure with that of a scorpion to swap bodies with Spider-Man. The Scorpion, meanwhile, in an attempt to go straight, was living with the Vulture. The Vulture, one of the originators of the Neogenic technology, believes he himself can use Silvermane's lab to make himself permanently youthful. During the Neogenic transfer, the Vulture intervenes, allowing Spider-Man, the Black Cat and the Scorpion to escape. Silvermane and the Vulture exchange energies in the neogenic transfer allowing the Vulture to become semi-permanently young again and reverting Silvermane back to an old man.

Alisa Silver (voiced by Leigh-Allyn Baker) is a former classmate of Peter Parker (Spider-Man). Peter briefly dated her. She was also the brilliant lab assistant of Dr. Connors (Lizard). But she was not as nice as everyone thought she was. She had a secret, namely that she was the daughter of Silvermane in this show. After her father used the Tablet of Time and was accidentally transformed into a baby, she took over her father's job as crime lord.

During her brief time as a crime boss she has hired and worked with Hammerhead, Tombstone, Ivonna, and the Vulture.

Alistair Smythe (voiced by Maxwell Caulfield) is the son of Spencer Smythe (voiced by Edward Mulhare). Spencer worked for Norman Osborn to create the Spider-Slayers in exchange that Oscorp makes the hoverchair for his son. Alistair later witnessed his father's failure to destroy Spider-Man (which, as in the comics, caused his apparent death) and was convinced to create Spider Slayers to later attack Spider-Man as part of a deal made with the Kingpin.

Smythe became Kingpin's primary accomplice for some time, even after Smythe's Slayers failed to kill Spider-Man. His association with the Kingpin came to an end after the two-parter "Framed/The Man Without Fear", when Spider-Man and Daredevil teamed up to fight the Kingpin, resulting in the arrest of Richard Fisk. Angered at his son's arrest, Kingpin blamed the incident on Smythe's "incompetence" and turned him against his will into the Ultimate Spider Slayer Cyborg with the help of the Kingpin's current replacement partner, Herbert Landon.

Breaking free of Kingpin's programming and recovering his father's body (which had been kept alive in stasis by the Kingpin unbeknownst to him initially), Alistair found another employer in Silvermane, who promised to fund research to revive his father. His father was never revived throughout the series. Smythe kidnapped the Black Cat for Silvermane to coerce Spider-Man into getting either Scorpion or Vulture, in order to have the de-aged Silvermane restored to an adult. However, this plan was foiled thanks to the team-up of Spider-Man, the Black Cat, the Scorpion and the Vulture.

Still under Silvermane's employment, he worked with Harry Osborn, the second Green Goblin, who had Smythe build an army of Goblin Warriors to attack Peter Parker and the clone of Mary Jane Watson's wedding, while Smythe also helped Scorpion with other activities. Harry's plan failed in the end thanks to Spider-Man, the Kingpin and the Black Cat.

Alistair also ordered Professor Miles Warren to create genetic clones under Silvermane's orders as revealed in the two-part episode, The Return of Hydro-Man, and worked for the Red Skull in the three-part episode, Secret Wars.

An alternate Smythe appeared in the two-part series finale of the series, working for the Kingpin and Spider-Carnage in order to build a mind-control device to take over the world. However, Spider-Carnage's real intentions were to destroy all reality. After Spider-Man foiled his plans, Spider-Carnage fled to another reality, leaving a bomb in the Kingpin's building. Though the Spider-Men and the Kingpin of that reality were saved by being teleported out of the exploding building, Smythe and most likely everyone else in the building were killed.

Tombstone (voiced by Dorian Harewood) was not originally an albino, but he was turned into one by the same laboratory accident that gave him his superpowers. He was hired by Silvermane to steal the Tablet of Time in his first two appearances, "Tablet of Time" and "Ravages of Time". During an altercation with Spider-Man in Dr. Conners's lab, poison gas was released, choking Spider-Man as Tombstone bearhugged him, who was unaffected because he claimed that he did not need to breathe anymore. In fact, it is implied that he is a walking corpse, a member of the undead. Later on in the series, in the episode "Tombstone", Tombstone appeared again and became the leader of a gang that had Robbie Robertson's son, Randy, as a member, to kill the story of Alicia Silvermane being a gangster in the Daily Bugle. Tombstone promised to set Randy free to Robbie if he kills the Silvermane story. Robbie refuses and Spider-Man captures Tombstone, having him sent to jail while Robbie and Randy confess to the police.

Also, in this series, Tombstone's origin was different in the comics. He was once Lonnie Lincoln, a friend of Joseph "Robbie" Robertson who was not an albino. When he was a child, he accidentally threw his basketball through the glass of a grocery store, but, after seeing the store was closed, Lonnie decided to rob the store to get ice cream with Robbie's help (when it ended up closed upon Robbie taking too long with his homework). Lonnie ended up failing when the police came and arrested him, whereas Robbie ran away prior to the poice's arrival. After his release, Lonnie became a crook while Robbie graduated with honors at schools. Eventually, Lonnie made a break-in at the Spaulding Chemical Plant to contact Robbie. While there, it was revealed that Lonnie wanted to frame Robbie to get his revenge for abandoning him back at the grocery store. After Lonnie fell into the vat of chemicals by accident, Robbie decided to stay behind to face the consequences to think about abandoning Lonnie at the grocery store. After the fall, Lonnie's skin turned white, his hair yellow, and his flesh tough and rock-like.

Later in the series, in the fourth-season premiere, "Guilty", it was revealed that the prison Tombstone went to was Ryker's Island with Richard Fisk (who was incarcerated two episodes before Tombstone). Fisk and Tombstone agreed to team up and frame Robbie Robertson, and Robbie was sent to prison at Ryker's thanks to the machinations of Fisk's father, the Kingpin. There, Robbie would live for the next fifteen years. He also met Richard and Tombstone there again and they revealed their entire plot to him. Apparently, Spider-Man found out about this whole plan with the help of J. Jonah Jameson and rescued Robbie just when Richard and Tombstone would escape with their cellmate, also putting them back to jail.

Like the comics' version of Venom, this version is a reporter named Eddie Brock (voiced by Hank Azaria). However unlike the comic story, Brock is introduced as an antagonist to Spider-Man prior to becoming Venom. Also the series made much greater strides at establishing the rivalry between the two as well as exemplifying Brock's motives, and linking him to other notable characters.

In the series, Brock is an ambitious reporter, but demonstrates quite a lack of integrity, and blames Spider-Man for his failures instead of his own poor judgement. Brock's story begins under the employment of newspaper owner J. Jonah Jameson. When he discovers that Dr. Curt Connors was the monstrous Lizard, he was eager to expose him for a Pulitzer-worthy story, and paid no consideration for Connors' family. But fortunately he was stopped by Spider-Man, who transformed Connors back to normal in time for Brock to look like a fool in front of Jameson. Later, Brock collaborated with Norman Osborn and Spencer Smythe to expose Spider-Man on live TV, by capturing and unmasking him. However, when the captured Spider-Man turned out to be Flash Thompson in his own costume for a prank, Jameson fires Brock due to the humiliation of faulty journalism.[1] Afterwards, Alistair Smythe attacks Brock, Osborn and Jameson with a newly rebuilt "Black Widow" Spider-Slayer, blaming them for the supposed death of his father Spencer, but they were rescued by Spider-Man. Despite this, the Slayer's destruction ended up costing Brock another job. Again Brock blames Spider-Man and swears revenge.[2]

In "The Alien Costume" three-parter, Brock manages to shoot pictures of Spider-Man at a crashed space shuttle, who was there to rescue the astronauts, John Jameson and his co-pilot. He attempts to frame Spider-Man to robbing the shuttle, which was actually robbed by the Rhino for the Kingpin. Jameson gives Brock his job back, and campaigned aggressively for Spider-Man's capture, until John Jameson reveals to his father about the man in the Rhino costume. Jameson retracts his campaign against Spider-Man and furiously fires Brock once again. Brock is never seen working for anyone again, and once again blames Spider-Man for his misfortune.

Soon, Brock would become bonded to an alien symbiote that was once attached to Spider-Man. The symbiote's original origin from the comics is altered. In this series, the alien was inadvertently brought back by John Jameson from his Moon-based space exploration. During their return, the astronauts are assaulted by the symbiote and crash their ship in the center of New York where Spider-Man arrives to help, inadvertently collecting the symbiote on his costume when he leaves. Brock, seeking revenge, follows Spider-Man to a church tower. Realizing that the symbiote costume's benefits are outweighed by the negative emotions it is creating, Spider-Man uses the sound of bells in the church tower to force it to leave his body, where it bonds with Brock below.[3]

Upon merging with the symbiote, Brock gains the knowledge of Peter Parker as Spider-Man as well as many of his abilities and declares that "From now on we're poison to you Spider-Man, that's why we call ourselves Venom." He also reveals to Spider-Man that he has vast knowledge of the universe but the symbiote's main goal is to survive. He seemingly has an advantage over Spider-Man during their conflict but, Brock's plan to exact revenge on Spider-Man comes to a halt when he is separated from the symbiote by Spider-Man, and taken to prison.[4] Yet he would later be reunited with both his symbiote and Spider-Man.

As a series adaptation of the original comic book stories, Venom's activities in the show are drastically different from the original written material. Despite the fact that he is a popular character, Venom was only featured in a handful of episodes (although he appears at the very end of the opening credits for each episode, even in seasons where he is not present). Brock's role as an antagonist changes, when he finds himself teaming with Spider-Man. In the episode "Carnage," when his symbiote's offspring known as Carnage, tries to take his girlfriend through a portal, Venom tackles Carnage, and he loses his grip on her. Both Brock and Venom are sucked in, his last words were that he had always loved her.

In his last appearance, a robotic copy of Venom and Carnage, reappear in "The Haunting of Mary Jane", before being blasted.

Adrian Toomes (voiced by Eddie Albert as an old man and Alan Johnson as a young man) is an inventor and engineer. In his first three appearances in "Ravages of Time", "Shriek of the Vulture" and "The Final Nightmare", he is an elderly man who develops strong grudge against Norman Osborn for rejecting and ridiculing his work. Later, he used his Vulture talons to suck youth out of people to temporarily make himself young. In "The Final Nightmare", after draining Spider-Man's youth, the Vulture unintentionally got Spider-Man's mutation disease. At the lab, Dr. Connors tricked him into holding this disease, which causes him to go into hiding.

In "Partners", it is shown that he somehow got rid of the mutation disease but then started changing from his young to old self (and vice versa), without control over this transformation. He also becomes young full-time by stealing baby Silvermane's youth, reverting Silvermane back to an old man. In the "Six Forgotten Warriors", part 1-5, Vulture became a member of the Insidious Six, replacing Mysterio, who was originally a member but died in an earlier episode.

The Beyonder (voiced by Earl Boen) in the cartoon greatly differs from the comics, as he sports a goatee as opposed to having a clean-shaven face. In all his appearances he also wore the brief "super hero" costume his comic counterpart Secret Wars II instead of the more usual disco outfit. He is also actually more of a good guy than a villain. The Beyonder first appeared in the episode "Arrival" of Season 5, the first chapter of that Secret Wars in the series. The Beyonder worked with Madame Web to prepare Spider-Man for the Secret Wars. When Doctor Doom was defeated in "Doom" (the third and last chapter to the Secret Wars) after the villain temporarily stole the Beyonder's powers, the Beyonder had all the heroes Spider-Man had summoned to assist him forget their actions, and sent them home. Spider-Man, however, was needed for something bigger, so he was the only one whose memory was untouched. It was revealed that the evil Spider-Carnage had destroyed the entire multiverse with a giant bomb. However, when the destruction reached the Beyonder's own reality, he used all of his powers to roll back time. Though it tested him to the extreme, he sent his servant, Madame Web, into the past to train Spider-Men from many different realities to find out which one would be worthy enough to lead the others against Spider-Carnage. In the series finale, he was growing weak because he had stayed too long out of his own reality, but had managed to find enough strength to teleport the six-armed Spider-Man, who had mutated into a Man-Spider, away back into another reality with him. That was the last time the Beyonder was seen in the series.

A stone gargoyle on top of a building whom Spidey talks to whenever he doubts himself. It is often thought that he got his name from Bruce Wayne, aka Batman. This is due to the fact that sometimes, Spider-Man mocks Bruce. However, John Semper revealed in an interview that Bruce was named after his good friend, Bruce Helper.

Billy Connors (voiced by Toby Scott Ganger) is the son of Margeret Connors and Dr. Curt Connors. He only appeared in the series premiere, Night of the Lizard.

Margaret Connors (voiced by Giselle Loren) is the wife of Dr. Curt Connors. She first appeared in the show's very first episode, Night of the Lizard, and her last notable appearance was in The Lizard King. Throughout most of her appearances, Margaret has been involved in one way or another in changing her husband, Curt, back into his human form from being the Lizard.

Calypso (voiced by Susan Beaubian) was initially introduced as a research scientist named Dr. Mariah Crawford, who was engaged to Sergei Kravinoff before his mystic serum transformed him into Kraven the Hunter. She assisted Spider-Man in several episodes, before she was given the serum herself by Kravinoff when she fell ill from an African plague. This transformed her into a feral woman who greatly resembled the comic version of Calypso except for having a lioness-like face and ears, different colored eyes, and claws. She was eventually half-cured by Spider-Man, the Black Cat and Kraven the Hunter and she refused to be cured all the way back to Dr. Mariah Crawford, running away with Kraven with the both of them with unknown fates.

During her appearances, she was called Calypso by Kravinoff even before she became the beast. Crawford explained to Spider-Man in the episode Duel of the Hunters that in Greek mythology, Calypso was a goddess who granted King Odysseus the gift of life.

Mrs. Farrel (voiced by Telma Hopkins) is Robert's mother. Only appears in the episode "Rocket Racer." Mrs. Farrell is a deeply caring and good woman, especially as a mother, to her son among others. She runs a small-time grocery store. She worries about her son for becoming the Rocket Racer, considering his unfavorable past as a street punk, and she believes, correctly though, that her son is a good, kind-hearted but misguided person. Her son's ultimate redemption as a street thug is worrying about his mom's physical health, which points that she might be considerably sick. When her store was threatened by thugs demanding protection money or else they will burn it down, her store was saved by Spider-Man and Robert.

Vanessa Fisk (voiced by Caroline Goodall) is the ex-wife of the Kingpin / Wilson Fisk. Vanessa loved her ex, Wilson Fisk, but hated his alter ego, the Kingpin. When Vanessa was kidnapped by the Kingpin's enemy force by Silvermane and she was brought back by Alistair Smythe's robot (Smythe was the Kingpin's current assistant at the time), she decided that she had enough of her husband's criminal life and left him, saying goodbye to him. Her later fate is unknown. Both she and Wilson had a son named Richard.

John Hardesky (voiced by John Phillip Law) appeared in the second and third episodes of Season 4. It is revealed that he is Felicia Hardy's father, and he was a crook known as the Cat, who was captured and taken into S.H.I.E.L.D., unbeknownst to Felicia who thought her father was deceased. But not only that but John also knew the supersoldier formula that created Captain America in World War II when John was a kid.

Later, Wilson Fisk, a.k.a. the Kingpin of Crime, found out that John Hardesky knew about the supersoldier formula, so the Kingpin had Hardesky abducted and the Chameleon posed as Hardesky and went in his cell. Kingpin sent Dr. Octopus to kidnap Felicia and threaten John if he did not reveal the supersoldier formula, Felicia would die. Hardesky told Kingpin about the formula and it was tested on Felicia off-screen where she became the Black Cat. She was originally a crook for the Kingpin to do his bidding, but she teamed up with Spider-Man and broke her father free from Kingpin and destroyed the files on the formula. At the end of episode "The Black Cat", Hardskey chooses to return to S.H.I.E.L.D. custody, so that no one can force him to reveal the formula again.

Anastasia Hardy (voiced by Rue McClanahan in the first appearance, Dimitra Arliss in season three, and Nita Talbot in season four) is the mother of Felicia. She is a businesswoman and a single mother as Felicia's father, a career jewel thief known as the Cat, had been imprisoned for years because he had memorized the World War II super soldier formula.

J. Jonah Jameson (voiced by Edward Asner) has a hatred of Spider-Man which is based less on his powers and more to his concealing his identity behind a mask; in this continuity, his wife was killed by a masked gunman. He also portrays his integrity as a journalist, refusing to cover up the truth even when it's in his best interests, and portrays his loyalty to those who work for him. He secretly hired attorney Matthew Murdock to defend Peter Parker when Peter was framed by Richard Fisk and personally uncovered evidence exonerating Robbie when he was framed by Tombstone and Richard Fisk.

In the high-tech Spider-Man's reality, J. Jonah Jameson doesn't hate Spider-Man and is more friendly towards him.

John Jameson (voiced by Michael Horton) appeared in The Alien Costume saga in which he unwittingly brings the Venom symbiote to Earth.

Stan Lee appeared in the series finale as himself in an alternate reality (this is supposed to be the reality the viewers live in). The powerless Spider-Man takes the Prime Spider-Man to his reality, in which he is an actor who plays Spider-Man. In this reality, Spider-Man is a comic book character and Stan Lee created him. The Prime Spider-Man takes him for a swing and then stop at the top of a building. Lee helps Spider-Man understand how the hero's life works based from his point of view as a writer, and Spider-Man thanks Stan Lee for creating him and making him a hero, then leaves after the conversation. Stan Lee then wonders how to get down from the building and hopes that the Fantastic Four might make an appearance and get him down.

Det. Terri Lee (voiced by Dawnn Lewis) was part of the NYPD that investigated cases that involved Spider-Man and to an extent Peter Parker, yet is unable to figure out the two are one and the same. At first, she had little trust for the web slinger, but over the course of the series she began to accept him as a worthy ally. She has ties to Cletus Kasady and has fallen for Blade. She is based on Captain Jean Dewolff.

Ned Leeds (voiced by Bob Bergen) appeared in a little cameo in the series. Peter Parker and Ned had no rivalry to win over Betty Brant, as in the series, she never appeared and her place was taken by Glory Grant. And also, Ned did not become hypnotized by the Hobgoblin into becoming a villain.

Microchip (voiced by Robert Axelrod) is a man whose nickname was Chip rather than Micro. He acted as the Punisher's accomplice and conscience, constantly urging him to use the non-lethal weaponry..

Uncle Ben (voiced by Brian Keith) appeared in the series as a spirit talking to Spider-Man whenever he was frustrated in a mission or missions. The spirit of Uncle Ben first appeared in Mysterio's debut episode. It was revealed, like in the comics, in the second episode of the third season that Ben was killed by a crook Peter let go from a wrestling rink, causing him to turn into Spider-Man.

In the series finale of the show, when Spider-Man had to stop the evil Spider-Carnage from destroying all reality, they were in a reality where Uncle Ben did not die and Spider-Man used him to make Spider-Carnage reformed. Spider-Carnage turned good and prevented the destruction of reality. Unfortunately, he could not get rid of the symbiote. So he created a portal that would cause anything inside of it disintegrate and jumped in, committing suicide. Although they lived in different realities, Ben saying that he is proud of his nephew and Spider-Man said that he will always have him in his heart, implying that Spider-Man had informed Ben of his counterpart's death.

Aunt May (voiced by Linda Gary in the first three seasons, Julie Bennett in the final two seasons) strongly disliked Spider-Man but loved his alter ego, her nephew Peter Parker. As in the comic, she does not know that her nephew is really Spider-Man. She had many encounters with villains Spider-Man fought like Venom, the Hobgoblin, Doctor Octopus, Morbius, the Chameleon (who was imitating Anna Watson), Tombstone, Green Goblin, the Scorpion and the Shocker. She has a past with Keane Marlow as friends and also, Keane was May's late husband, Ben's friend as well (and unbeknownst to her, he was also a member in the 1940s superhero team called the Six Forgotten Warriors being named the Destroyer). May has a friend relationship with Anna Watson, and despite they like each other and May has some kindness to Mary Jane, Anna has no respect for Peter and he has to tolerate her (though there was a time when Peter blurted out to her in anger where she blamed him for Mary Jane getting kidnapped by the clone of the dead Hydro-Man) and Anna even once had respect for him when he was with Mary Jane after she was saved from the Hydro-Man clone. May even gave Peter and Mary Jane her wedding rings when she had with Uncle Ben before his death when Peter and M.J. were getting married.

By the series finale of the show, it was revealed that when the Beyonder and Madame Web rounded up Spider-Men from different realities, that in the Scarlet Spider and Spider-Carnage's reality, Aunt May was dead with Uncle Ben, but her cause of death was unknown.

Richard and Mary Parker are Peter Parker's parents who both died when he was very young. They are only rarely mentioned in the series and only once appear, to Peter in a vision via the sorcery of Baron Mordo.

Hobie Brown (voiced by Tim Russ) appeared in the episode The Prowler. He first appeared working for a crime lord called Iceberg and was feeling he was not earning his fair cut for the work he was doing. When Iceberg found out, he had his boys attempt to kill Hobie. After narrowly escaping that fate, he knew he needed to get out of town. He stole a passer-by's purse in order to fund his new trip. It turned out to be Mary Jane's purse when she and Peter Parker are in the process of apartment hunting but was stopped by Spider-Man and Hobie was sent to jail for violating his parole. While in jail, he saves Richard Fisk from an attempt on his life. As payment, the Kingpin arranges for a hot shot lawyer to enable Hobie's release and gives him a special suit. He used the costume to get back at his old boss Iceberg. There are strings attached, however: the belt will detonate if he tampers with it. He and Spider-Man team up against the Kingpin and destroyed the controls to the belt so it would not detonate and Prowler's suit is recharged. Hobie gave up crime so he can have a chance at returning to a normal 'good' life.

Rocket Racer (voiced by Billy 'Pop' Atmore) only appears in the episode "Rocket Racer". Robert had great skill in gyro mechanicisms due to working at the Science center taught by Peter Parker. He created a cybernetically controlled, rocket-powered magnetic skateboard which he travels with and can attain speeds of 60 miles per hour (97 km/h). He can also scale walls. After Robert stole some jewels, he was chased by Spider-Man. Rocket Racer escaped but Spider-Man planted a tracer on him and found out his identity.

A criminal leader, whose machines were stolen, created the Big Wheel, a giant wheel with lasers, missiles, and spikes that was able to roll onto buildings and higher grounds. Spider-Man and Rocket Racer were able to stop the Big Wheel when Rocket Racer had Spider-Man electrocute the Big Wheel with two electric wires. The criminal was arrested. This taught Robert that education, not crime, would help him in the world to overcome his problems. He even helped Spider-Man defeat some thugs that plotted to burn down his mother's store due to lack of protection money.

In the 1990s' Spider-Man, Robertson (voiced by Rodney Saulsberry) was J. Jonah Jameson's right-hand man and the person trying to have Jameson see that Spider-Man is not evil. And also like in the comics, he was a former friend of Lonny Lincoln, also known as Tombstone. Back when Robbie and Lonny were kids, they last see each other when they wanted to get some ice cream but unfortunately, it was closed. Then Lonny accidentally threw his basketball through a window and he insisted that Robbie would help him get to the cold junk food. Robbie boosted him up but he knew the police would come. So he quickly abandoned him while Lonny was arrested. Robbie felt guilty about leaving Lonny on that point on. By the time Robbie got a job at a local newspaper years later, he would investigate about the Spalding Chemical Plant. He found Lonny, now a crook, there so Robbie can be put to jail so he can have his revenge for abandoning him at the grocery store. Robbie tried to catch him but Lonny unfortunately slipped and fell into a chemical pool where Robbie thought he died. In order to mend his mistakes back years ago since the grocery store, he decided to stay until he let the police hear his story and they let him go when the people at the newspaper company vouched for him.

By the time he became Jameson's right-hand man, Robbie was horrified to figure out his son, Randy, is in a thug gang called the Posse. And worse, he found out that Lonny managed to survive falling into the chemical pool but turned into an ugly mutant known as Tombstone, who also led the Posse.

In the end, Spider-Man and Robbie teamed up to put Tombstone in jail and also have Randy figure out the Posse was no place for him. But by the fourth-season premiere of Spider-Man, when Tombstone was incarcerated in Rooker's Island and wanted to get revenge on Robbie for what he did, he teamed up with Richard Fisk, who was also incarcerated in Rooker's and was jailed earlier before Tombstone, and with Richard's connections, they had Robbie framed and taken to the same prison they were so Tombstone can kill Robbie. But Spider-Man and J. Jonah Jameson saved Robbie and cleared his name.

By the Six Forgotten Warriors saga at the fifth (and final) season of this show, Peter Parker lied to J. Jonah Jameson that Spider-Man was going to Russia to rule the world. Jameson believed him and Robbie went with Peter to accompany him and make sure he gets some pictures. Robbie acted weird while in Russia because he knew some people who would explain about Peter's parents when they were thought to be traitors to Russia. Robbie even knew Omar Mosley, who knew the story about the Six Forgotten Warriors. One of the Six Forgotten Warriors' members was the Black Marvel (who was said to be dead) and Omar said he was the Black Marvel's sidekick (but secretly, Omar was the Black Marvel himself). Robbie even figured out that Wilson Fisk was the Kingpin.

By the series finale of the show, in the Scarlet Spider's reality, all of New York was destroyed with nearly everyone dead, thanks to the murderous rampage the Green Goblin and Hobgoblin have committed. The only survivors were J. Jonah Jameson and Robbie. Hobgoblin threatened Jameson if he did not give the broadcaster on the roof of J3 Communications, then he would drop Robbie off a ledge. Spider-Man saved Robbie but surprisingly, Robbie hated Spider-Man as much as Jameson did because here, in this reality, Spider-Man was Spider-Carnage and he was the boss of the Green Goblin and Hobgoblin.

Martha Robertson (voiced by Iona Morris) is Joseph "Robbie" Robertson's wife and the mother of Randy Robertson. She first appeared in the episode Tombstone, where Randy had joined a crime gang called the Posse led by a childhood friend of Robbie's. She later appeared in the episode Guilty, where Robbie had been framed by Tombstone and Richard Fisk, although Robbie was soon released.

Randy (voiced by Alfonso Ribeiro) first appeared in the episode Tombstone, where he began leading a life of crime in a gang led by Tombstone called the Posse. It later appeared that Tombstone had been a childhood friend of his father. Spider-Man discovered that Randy was in this gang and Tombstone later revealed this to Robbie, in the end, Spider-Man managed to reassure Randy that the Posse was no place for him, and Tombstone was imprisoned. Randy appeared in the episode Guilty when Robbie had been framed and imprisoned by Tombstone and his cell-mate Richard Fisk, although Robbie was soon released.

Silver Sable (voiced by Mira Furlan) has appeared in the Six Forgotten Warriors saga. The Wild Pack accompanied her, and she served as both an ally and enemy to Spider-Man against both the Kingpin and the Red Skull.

The Spider Slayers were a line of robots being commissioned by Norman Osborn for hunting Spider-Man. This culminated in Alistair Smythe working for the Kingpin; he would go on to not only recreate the "Black Widow" Spider-Slayer, but also at the same time to unleash the "Tarantula" and "Scorpion" Spider-Slayers. In this depiction, the three Spider-Slayers could join together as a massive engine of destruction. Later, Alistair would build another Slayer called the "Mega Slayer", a heavily armed humanoid robot operated by remote. Alistair left the Kingpin's service after, as punishment for repeated failures, the Kingpin's new chief scientist, the mutate Herbert Landon, genetically mutated him into the "Ultimate Spider-Slayer". In this form, Alistair was capable of walking again and was incredibly strong and resilient, enough to restrain Spider-man in two crushing bearhugs and even take on the Kingpin. He also had twin horns growing from his shoulders, both of which were organic laser guns.

The Spot (voiced by Oliver Muirhead) and Dr. Silvia Lopez (voiced by Wanda De Jesus) appear in the episode titled, "The Spot". In this episode, Jonathan Ohn is fired from his work by Tony Stark (Iron Man) for Ohn's work involves creating dimensional portals. Since the events of the episodes Venom Returns and Carnage, Stark thought it would be best if Ohn's work were shut down.

The Kingpin locates Ohn, pretending to be appreciate Ohn's job of creating portals, and gives him a job with a woman whom he loves, Dr. Silvia Lopez. As weeks pass, Ohn and Lopez finish their work but Ohn falls into one of his portals, becoming the Spot when a whole group of portals attached to him. He can create portals with his mind. The Kingpin was frustrated with Ohn and considered him and Lopez very vague. The Spot used his portal-creating abilities to rob banks and jewelry stores to get more money to fund his research. He then deduces that he works for a criminal like the Kingpin and wants to take over the empire. However, the Kingpin uses Lopez to have the Spot capture and kill Spider-Man. The Spot confronts Spider-Man but the hero defeats him. Spider-Man is aware that if the Spot fails, Lopez would die and the two start to work together. In a battle to defeat the Kingpin, Ohn opens a portal that grows so big it had the potential to engulf the Earth, starting with New York City. Spider-Man, the Spot, Lopez and Kingpin work together to take down the portal. However, the Spot cannot completely close the portal from the outside. He jumps in but not before Lopez expresses her love for him. They were both sucked in. Spot is voiced by Oliver Muirhead.

An alternate reality version of the Spot appears in part one of the series finale in a cameo as Dr. Ohn, when the Scarlet Spider explains the origin of Spider-Carnage. In this reality, Ohn works for the Kingpin and accidentally releases the Carnage symbiote through one of his portals.

Gwen Stacy (voiced by Mary Kay Bergman) appears in the series finale, when Spider-Man visits a parallel universe, in which Peter Parker (Armored Spider-Man) is a wealthy industrialist similar to Iron Man. Gwen Stacy is his fiancée, and Spider-Man reflects that his alternate self is engaged to a woman he does not even know (as he never met his reality's Gwen Stacy). Spider-Carnage who wants destroy every parallel reality kidnaps her, threatening to everyone present if one of them follows him, she will die first before he destroys all reality.

Spider-Man then understands that Spider-Carnage is not truly evil as he is confused because in his reality, his Uncle Ben and Aunt May have died and he is believed to be a clone to the Scarlet Spider, also known as Ben Reilly. So, Spider-Man then goes to the Uncle Ben who was alive in this reality (Since this world's Spider-Man has never failed at anything, Uncle Ben was still alive) and tells him everything about Spider-Carnage's plan. Meanwhile, Spider-Carnage tells Gwen on how he will destroy all reality. Spider-Man and Uncle Ben then arrive and Ben reforms Spider-Carnage while Spider-Man frees Gwen. Spider-Carnage prevents the destruction of all reality but unfortunately cannot remove the Carnage symbiote. Gwen then witnesses as Spider-Carnage jumps into an unbalanced portal, killing himself.

In this universe, Gwen Stacy is alive and engaged to a very successful but arrogant Peter Parker, who boasts that he never fails. This is an opposite of her real fate in the iconic story arc, The Night Gwen Stacy Died, from the Amazing Spider-Man comics, where Spider-Man fails to save Gwen's life in his battle against the Green Goblin.

Farley Stillwell (voiced by Michael Rye) was the scientist that led the experiment involving neogenics, an accident during this enabled Peter Parker to become Spider-Man. He also took part in the creation of the Scorpion. At the end of season two, he disappears into the shadows, never to be seen again.

Flash Thompson (voiced by Patrick Labyorteaux) appeared in the series as an Empire State University student. While he had a contentious dislike of Peter Parker he greatly admired Spider-Man, willing to defend him verbally and became invariably involved in his conflicts. Flash initially pursued a relationship with Felicia Hardy, which did not work out and he later found a new, more comfortable, relationship with the brainy Debra Whitman. He was voiced by Patrick Labyorteaux.

Maria Taina Elizondo (voiced by Stephanie Eustase) first appeared in the Make a Wish episode in which she meets Spider-Man, and Spider-Man shares his origin with her. At the end of the episode, after the battle with Dr. Octopus Spider-Man is captured and brainwashed by Dr. Octopus. In the next episode, Taina, along with the help of a kind cab driver Mousie (voiced by Anne-Marie Johnson), tries to stop Spider-Man and help him realize who he truly is. At the end of the Attack of the Octobot episode, it shows Taina lives at the Wish Come True Foundation for Terminally Ill Children, implying that Taina is dying.

Miles Warren (voiced by Jonathan Harris) is a scientist is a cloning specialist, but the government banned his experiments. Warren's clones keep dissolving into vapour, because of their DNA. Warren would take interest in the super-villain Hydro-Man, who had the ability to transform himself into water. After finding a sample of his DNA, he creates a clone of Hydro-Man. But the clone becomes violent without Mary Jane Watson, and demands that Warren creates a clone of her. In the end of the final part of The Return of Hydro-Man, the clones of Hydro-Man and Mary Jane Watson degenerated. However, Warren was able to steal a sample of Spider-Man's DNA, in a possible attempt to clone him. It is also revealed that he works for the crime lord Silvermane who he kept anonymous from Spider-Man.

Spider-Man later visited an alternate universe in which Warren had managed to create a Spider-Man clone, which had escaped. In this reality, the current Spider-Man found out about the clone, and reason led himself to believe he was the clone. So he dyed his hair blond and called himself the Scarlet Spider and changed his name to Ben Reilly. The other Spider-Man, the clone, was bonded with Carnage symbiote and became Spider-Carnage.

Mary Jane Watson (voiced by Sara Ballantine) first meets a 19-year-old Peter Parker in "The Return of the Spider-Slayers" (Season #1 Ep #4). Mary Jane gradually supplants Felicia Hardy as his primary love interest. She is last seen in Season #3 Episode 41, "Turning Point," in which the Green Goblin discovers Spider-Man's true identity. In a nod to The Night Gwen Stacy Died, he takes her to the George Washington Bridge. Spider-Man tries to save her, but she falls into a dimensional portal created by his stolen time dilation accelerator, but Spider-Man did not know about her falling into the time dilation. In Season 4, a clone of Mary Jane created by Miles Warren appears, and after the death of Mysterio at the end of the ninth episode of Season 4, Spider-Man revealed he was Peter to her and M.J. and Peter marry in the first episode of Season 5. Later, her true nature is revealed and she and a clone of Hydro-Man dissolve due to their unstable cellular structure.

In the high-tech Spider-Man's reality, Mary Jane is still friends with Peter Parker.

At the end of the Spider Wars storyline, the series ends with Spider-Man going with Madame Web to search for the real Mary Jane so that he can finally be reunited.

Debra Whitman (voiced by Liz Georges) was a prominent supporting character and a love interest to Flash Thompson. An ESU genetics major, she is portrayed as an extremely sharp intellectual. Unlike her comic appearance she has no romantic relationship with Peter Parker, who considered her as his sister, though he sometimes found her annoying.

Glory Grant (voiced by Nell Carter) is the secretary of J. Jonah Jameson's Daily Bugle.

Agent X (voiced by Rachel Davies) is a S.H.I.E.L.D. intelligence agent. She took part in the mission to capture Chameleon when the latter was on a mission to disrupt an international peace treaty. The character is clearly Black Widow, likely renamed to avoid confusion with one of the Spider Slayer robots.