Pseudouridylate synthase
In enzymology, a pseudouridylate synthase (EC 4.2.1.70) is an enzyme that catalyzes the chemical reaction
- uracil + D-ribose 5-phosphate pseudouridine 5'-phosphate + H2O
| pseudouridylate synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 4.2.1.70 | ||||||||
| CAS number | 9023-35-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are uracil and D-ribose 5-phosphate, whereas its two products are pseudouridine 5'-phosphate and H2O.
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is uracil hydro-lyase (adding D-ribose 5-phosphate pseudouridine-5'-phosphate-forming). Other names in common use include pseudouridylic acid synthetase, pseudouridine monophosphate synthetase, 5-ribosyluracil 5-phosphate synthetase, pseudouridylate synthetase, upsilonUMP synthetase, and uracil hydro-lyase (adding D-ribose 5-phosphate). This enzyme participates in pyrimidine metabolism.
Structural studies
As of late 2007, 22 structures have been solved for this class of enzymes, with PDB accession codes 1DJ0, 1K8W, 1KSK, 1KSL, 1KSV, 1PRZ, 1QYU, 1R3E, 1R3F, 1SB7, 1SGV, 1SI7, 1SZW, 1V9F, 1V9K, 1VIO, 1XPI, 1Z2Z, 1ZE1, 1ZE2, 1ZL3, and 2I82.
References
- HEINRIKSON RL, GOLDWASSER E (1964). "Studies on the Biosynthesis of 5-Ribosyluracil 5'-Monophosphate in Tetrahymena Pyriformis". J. Biol. Chem. 239: 1177–87. PMID 14165924.
- Matsushita T, Davis FF (1971). "Studies on pseudouridylic acid synthetase from various sources". Biochim. Biophys. Acta. 238 (2): 165–73. doi:10.1016/0005-2787(71)90082-7. PMID 4936431.
- Remsen JF, Matsushita T, Chirikjian JG, Davis FF (1972). "Enzymatic synthesis of deoxypseudouridylic acid and a study of certain of its properties". Biochim. Biophys. Acta. 281 (4): 481–7. doi:10.1016/0005-2787(72)90148-7. PMID 4569284.
- Suzuki T, Hochster RM (1966). "On the biosynthesis of pseudouridine and of pseudouridylic acid in Agrobacterium tumefaciens". Can. J. Biochem. 44 (2): 259–72. doi:10.1139/o66-029. PMID 5942965.