Exocytosis

Exocytosis (/ˌɛkssˈtsɪs/[1][2]) is a form of active transport and bulk transport in which a cell transports molecules (e.g., neurotransmitters and proteins) out of the cell (exo- + cytosis) by secreting them through an energy-dependent process. Exocytosis and its counterpart, endocytosis, are used by all cells because most chemical substances important to them are large polar molecules that cannot pass through the hydrophobic portion of the cell membrane by passive means. Exocytosis is the process by which a large amount of molecules are released; thus it is a form of bulk transport. Exocytosis occurs via secretory portals at the cell plasma membrane called porosomes. Porosomes are permanent cup-shaped lipoprotein structure at the cell plasma membrane, where secretory vesicles transiently dock and fuse to release intra-vesicular contents from the cell.

Neuron A (transmitting) to neuron B (receiving)
1. Mitochondrion
2 Synaptic vesicle with neurotransmitters
3. Autoreceptor
4. Synapse with neurotransmitter released (serotonin)
5. Postsynaptic receptors activated by neurotransmitter (induction of a postsynaptic potential)
6. Calcium channel
7. Exocytosis of a vesicle
8. Recaptured neurotransmitter
Phagocytosis versus exocytosis

In exocytosis, membrane-bound secretory vesicles are carried to the cell membrane, where they dock and fuse at porosomes and their contents (i.e., water-soluble molecules) are secreted into the extracellular environment. This secretion is possible because the vesicle transiently fuses with the plasma membrane. In the context of neurotransmission, neurotransmitters are typically released from synaptic vesicles into the synaptic cleft via exocytosis; however, neurotransmitters can also be released via reverse transport through membrane transport proteins.

Exocytosis is also a mechanism by which cells are able to insert membrane proteins (such as ion channels and cell surface receptors), lipids, and other components into the cell membrane. Vesicles containing these membrane components fully fuse with and become part of the outer cell membrane.

History

The term was proposed by De Duve in 1963.[3]

Types

In eukaryotes there are two types of exocytosis: 1) Ca2+ triggered non-constitutive (i.e., regulated exocytosis) and 2) non-Ca2+ triggered constitutive (i.e., non-regulated). Ca2+ triggered non-constitutive exocytosis requires an external signal, a specific sorting signal on the vesicles, a clathrin coat, as well as an increase in intracellular calcium. Exocytosis in neuronal chemical synapses is Ca2+ triggered and serves interneuronal signalling. Constitutive exocytosis is performed by all cells and serves the release of components of the extracellular matrix or delivery of newly synthesized membrane proteins that are incorporated in the plasma membrane after the fusion of the transport vesicle.

Vesicular exocytosis in prokaryote gram negative bacteria is a third mechanism and latest finding in exocytosis. The periplasm is pinched off as bacterial outer membrane vesicles (OMVs) for translocating microbial biochemical signals into eukaryotic host cells[4] or other microbes located nearby,[5] accomplishing control of the secreting microbe on its environment - including invasion of host, endotoxemia, competing with other microbes for nutrition, etc. This finding of membrane vesicle trafficking occurring at the host-pathogen interface also dispels the myth that exocytosis is purely a eukaryotic cell phenomenon.[6]

Steps

Five steps are involved in exocytosis:

Vesicle trafficking

Certain vesicle-trafficking steps require the transportation of a vesicle over a moderately small distance. For example, vesicles that transport proteins from the Golgi apparatus to the cell surface area, will be likely to use motor proteins and a cytoskeletal track to get closer to their target. Before tethering would have been appropriate, many of the proteins used for the active transport would have been instead set for passive transport, because the Golgi apparatus does not require ATP to transport proteins. Both the actin- and the microtubule-base are implicated in these processes, along with several motor proteins. Once the vesicles reach their targets, they come into contact with tethering factors that can restrain them.

Vesicle tethering

It is useful to distinguish between the initial, loose tethering of vesicles to their objective from the more stable, packing interactions. Tethering involves links over distances of more than about half the diameter of a vesicle from a given membrane surface (>25 nm). Tethering interactions are likely to be involved in concentrating synaptic vesicles at the synapse.

Tethered vesicles are also involved in regular cell's transcription processes.

Vesicle docking

Secretory vesicles transiently dock and fuse at the porosome at the cell plasma membrane, via a tight t-/v-SNARE ring complex.

Vesicle priming

In neuronal exocytosis, the term priming has been used to include all of the molecular rearrangements and ATP-dependent protein and lipid modifications that take place after initial docking of a synaptic vesicle but before exocytosis, such that the influx of calcium ions is all that is needed to trigger nearly instantaneous neurotransmitter release. In other cell types, whose secretion is constitutive (i.e. continuous, calcium ion independent, non-triggered) there is no priming.

Vesicle fusion

Transient vesicle fusion is driven by SNARE proteins, resulting in release of vesicle contents into the extracellular space (or in case of neurons in the synaptic cleft).

The merging of the donor and the acceptor membranes accomplishes three tasks:

  • The surface of the plasma membrane increases (by the surface of the fused vesicle). This is important for the regulation of cell size, e.g., during cell growth.
  • The substances within the vesicle are released into the exterior. These might be waste products or toxins, or signaling molecules like hormones or neurotransmitters during synaptic transmission.
  • Proteins embedded in the vesicle membrane are now part of the plasma membrane. The side of the protein that was facing the inside of the vesicle now faces the outside of the cell. This mechanism is important for the regulation of transmembrane and transporters.

Vesicle retrieval

Retrieval of synaptic vesicles occurs by endocytosis. Most synaptic vesicles are recycled without a full fusion into the membrane (kiss-and-run fusion) via porosome. Non-constitutive exocytosis and subsequent endocytosis are highly energy expending processes, and thus, are dependent on mitochondria.[7]

Examination of cells following secretion using electron microscopy demonstrate increased presence of partially empty vesicles following secretion. This suggested that during the secretory process, only a portion of the vesicular content is able to exit the cell. This could only be possible if the vesicle were to temporarily establish continuity with the cell plasma membrane at porosomes, expel a portion of its contents, then detach, reseal, and withdraw into the cytosol (endocytose). In this way, the secretory vesicle could be reused for subsequent rounds of exo-endocytosis, until completely empty of its contents.[8]

References

  1. "Exocytosis". Oxford Dictionaries UK Dictionary. Oxford University Press. Retrieved 2016-01-21.
  2. "Exocytosis". Merriam-Webster Dictionary. Retrieved 2016-01-21.
  3. Rieger, R.; Michaelis, A.; Green, M.M. 1991. Glossary of Genetics. Classical and Molecular (Fifth edition). Springer-Verlag, Berlin, .
  4. YashRoy R C (1993) Eelectron microscope studies of surface pili and vesicles of Salmonella 3,10:r:- organisms. Indian Journal of Animal Sciences, vol. 63, pp. 99-102.https://www.researchgate.net/publication/230817087_Electron_microscope_studies_of_surface_pilli_and_vesicles_of_Salmonella_310r-_organisms?ev=prf_pub
  5. Kadurugamuwa, J L; Beveridge, T J (1996). "Bacteriolytic effect of membrane vesicles from Pseudomonas aeruginosa on other bacterial including pathogens: conceptually new antibiotics". Journal of Bacteriology. 178 (10): 2767–2774. doi:10.1128/jb.178.10.2767-2774.1996. PMC 178010. PMID 8631663.
  6. YashRoy, R.C. (1998). "Discovery of vesicular exocytosis in procaryotes and its role in Salmonella invasion" (PDF). Current Science. 75 (10): 1062–1066.
  7. Ivannikov, M.; et al. (2013). "Synaptic vesicle exocytosis in hippocampal synaptosomes correlates directly with total mitochondrial volume". J. Mol. Neurosci. 49 (1): 223–230. doi:10.1007/s12031-012-9848-8. PMC 3488359. PMID 22772899.
  8. Boron, WF & Boulpaep, EL (2012), Medical Physiology. A Cellular and Molecular Approach, 2, Philadelphia: Elsevier

See also

This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.