Anthranilate 3-monooxygenase (deaminating)
In enzymology, an anthranilate 3-monooxygenase (deaminating) (EC 1.14.13.35) is an enzyme that catalyzes the chemical reaction
- anthranilate + NADPH + H+ + O2 2,3-dihydroxybenzoate + NADP+ + NH3
| anthranilate 3-monooxygenase (deaminating) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.13.35 | ||||||||
| CAS number | 37256-68-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The 4 substrates of this enzyme are anthranilate, NADPH, H+, and O2, whereas its 3 products are 2,3-dihydroxybenzoate, NADP+, and NH3.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is anthranilate,NADPH:oxygen oxidoreductase (3-hydroxylating, deaminating). Other names in common use include anthranilate hydroxylase, anthranilate 2,3-dioxygenase (deaminating), anthranilate hydroxylase (deaminating), anthranilic hydroxylase, and anthranilate 2,3-hydroxylase (deaminating). This enzyme participates in 3 metabolic pathways: benzoate degradation via hydroxylation, carbazole degradation, and nitrogen metabolism.
References
- Powlowski JB, Dagley S, Massey V, Ballou DP (1987). "Properties of anthranilate hydroxylase (deaminating), a flavoprotein from Trichosporon cutaneum". J. Biol. Chem. 262 (1): 69–74. PMID 3793735.
- Subramanian V, Vaidyanathan CS (1984). "Anthranilate hydroxylase from Aspergillus niger: new type of NADPH-linked nonheme iron monooxygenase". J. Bacteriol. 160 (2): 651–5. PMC 214784. PMID 6501219.