Genetically modified tree

A genetically modified tree (GMt, GM tree, genetically engineered tree, GE tree or transgenic tree) is a tree whose DNA has been modified using genetic engineering techniques. In most cases the aim is to introduce a novel trait to the plant which does not occur naturally within the species. Examples include resistance to certain pests, diseases, environmental conditions, and herbicide tolerance, or the alteration of lignin levels in order to reduce pulping costs.

Technician checks on genetically modified peach and apple "orchards". Each dish holds experimental trees grown from lab-cultured cells to which researchers have given new genes. Source: USDA.

Genetically modified forest trees are not yet approved ("deregulated") for commercial use, with the exception of insect-resistant poplar trees in China.[1][2] and one case of GM Eucalyptus in Brazil.[3] Several genetically modified forest tree species are undergoing field trials for deregulation, and much of the research is being carried out by the pulp and paper industry, primarily with the intention of increasing the productivity of existing tree stock.[4] Certain genetically modified orchard tree species have been deregulated for commercial use in the United States including the papaya and plum.[5] The development, testing and use of GM trees remains at an early stage in comparison to GM crops.[6]

Research

Research into genetically modified trees has been ongoing since 1988.[7] Concerns surrounding the biosafety implications of releasing genetically modified trees into the wild have held back regulatory approval of GM forest trees. This concern is exemplified in the Convention on Biological Diversity's stance:

The Conference of the Parties, Recognising the uncertainties related to the potential environmental and socio-economic impacts, including long term and trans-boundary impacts, of genetically modified trees on global forest biological diversity, as well as on the livelihoods of indigenous and local communities, and given the absence of reliable data and of capacity in some countries to undertake risk assessments and to evaluate those potential impacts, recommends parties to take a

precautionary approach when addressing the issue of genetically modified trees.[8]

A precondition for further commercialization of GM forest trees is likely to be their complete sterility.[6][9] Plantation trees remain phenotypically similar to their wild cousins in that most are the product of no more than three generations of artificial selection, therefore, the risk of transgene escape by pollination with compatible wild species is high.[10] One of the most credible science-based concerns with GM trees is their potential for wide dispersal of seed and pollen.[11] The fact that pine pollen travels long distances is well established, moving up to 3,000 kilometers from its source.[12] Additionally, many tree species reproduce for a long time before being harvested.[13] In combination these factors have led some to believe that GM trees are worthy of special environmental considerations over GM crops.[14] Ensuring sterility for GM trees has proven elusive, but efforts are being made.[15] While tree geneticist Steve Strauss predicted that complete containment might be possible by 2020, many questions remain.[16]

Proposed uses

GM trees under experimental development have been modified with traits intended to provide benefit to industry, foresters or consumers. Due to high regulatory and research costs, the majority of genetically modified trees in silviculture consist of plantation trees, such as eucalyptus, poplar, and pine.

Lignin alteration

Several companies and organisations (including ArborGen,[17] GLBRC,[18] ...) in the pulp and paper industry are interested in utilizing GM technology to alter the lignin content of plantation trees (particularly eucalyptus and poplar trees[19]). It is estimated that reducing lignin in plantation trees by genetic modification could reduce pulping costs by up to $15 per cubic metre.[20] Lignin removal from wood fibres conventionally relies on costly and environmentally hazardous chemicals.[21] By developing low-lignin GM trees it is hoped that pulping and bleaching processes will require fewer inputs,[22] therefore, mills supplied by low-lignin GM trees may have a reduced impact on their surrounding ecosystems and communities.[23] However, it is argued that reductions in lignin may compromise the structural integrity of the plant, thereby making it more susceptible to wind, snow, pathogens and disease,[24] which could necessitate pesticide use exceeding that on traditional plantations.[25] This has proven correct, and an alternative approach followed by the University of Columbia was developed. This approach was to introduce chemically labile linkages instead (by inserting a gene from the plant Angelica sinensis ), which allows the lignin to break down much more easy.[26] Due to this new approach, the lignin from the trees not only easily breaks apart when treated with a mild base at temperatures of 100 degrees C, but the trees also maintained their growth potential and strength.[27]

Frost tolerance

Genetic modification can allow trees to cope with abiotic stresses such that their geographic range is broadened.[28] Freeze-tolerant GM eucalyptus trees for use in southern US plantations are currently being tested in open air sites with such an objective in mind. ArborGen, a tree biotechnology company and joint venture of pulp and paper firms Rubicon (New Zealand), MeadWestvaco (US) and International Paper (US)[29] is leading this research.[30] Until now the cultivation of eucalyptus has only been possible on the southern tip of Florida, freeze-tolerance would substantially extend the cultivation range northwards.[31]

Reduced vigour

Orchard trees require a rootstock with reduced vigour to allow them to remain small. Genetic modification could allow the elimination of the rootstock, by making the tree less vigorous, hence reducing its height when fully mature. Research is being done into which genes are responsible for the vigour in orchard trees (such as apples, pears, ...).[32][33]

Accelerated growth

In Brazil, field trials of fast growing GM eucalyptus are currently underway, they are set to conclude in 2015–2016 with commercialization to result.[34] FuturaGene, a biotechnology company owned by Suzano, a Brazilian pulp and paper company, has been leading this research. Stanley Hirsch, chief executive of FuturaGene has stated: "Our trees grow faster and thicker. We are ahead of everyone. We have shown we can increase the yields and growth rates of trees more than anything grown by traditional breeding."[35] The company is looking to reduce harvest cycles from 7 to 5.5 years with 20-30% more mass than conventional eucalyptus.[35] There is concern that such objectives may further exacerbate the negative impacts of plantation forestry. Increased water and soil nutrient demand from faster growing species may lead to irrecoverable losses in site productivity and further impinge upon neighbouring communities and ecosystems.[36][37][38] Researchers at the University of Manchester's Faculty of Life Sciences modified two genes in poplar trees, called PXY and CLE, which are responsible for the rate of cell division in tree trunks. As a result, the trees are growing twice as fast as normal, and also end up being taller, wider and with more leaves.[39]

Disease resistance

Ecologically motivated research into genetic modification is underway. There are ongoing schemes that aim to foster disease resistance in trees such as the American chestnut[40] (see Chestnut blight) and the English elm[41] (see Dutch elm disease) for the purpose of their reintroduction to the wild. Specific diseases have reduced the populations of these emblematic species to the extent that they are mostly lost in the wild. Genetic modification is being pursued concurrently with traditional breeding techniques in an attempt to endow these species with disease resistance.[42]

Current uses

Poplars in China

In 2002 China's State Forestry Administration approved GM poplar trees for commercial use.[43] Subsequently, 1.4 million Bt (insecticide) producing GM poplars were planted in China. They were planted both for their wood and as part of China's 'Green Wall' project, which aims to impede desertification.[44] Reports indicate that the GM poplars have spread beyond the area of original planting [45] and that contamination of native poplars with the Bt gene is occurring.[46] There is concern with these developments, particularly because the pesticide producing trait may impart a positive selective advantage on the poplar, allowing it a high level of invasiveness.[47]

See also

References
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  2. Sedjo, R.A. (2005). "Will Developing Countries be the Early Adopters of Genetically Engineered Forests?" (PDF). AgBioForum. 8 (4): 205.
  3. "Brazil approves transgenic eucalyptus". Nature Biotechnology. 33 (6): 577. 9 June 2015. doi:10.1038/nbt0615-577c. PMID 26057961.
  4. Sedjo, R.A. (2010). "Transgenic Trees for Biomass: The Effects of Regulatory Restrictions and Court Decisions on the Pace of Commercialization" (PDF). AgBioForum. 13 (4): 391.
  5. Sedjo, R.A. (2010). "Transgenic Trees for Biomass: The Effects of Regulatory Restrictions and Court Decisions on the Pace of Commercialization" (PDF). AgBioForum. 13 (4): 393.
  6. Kanowski, Peter. "Genetically-modified trees: opportunities for dialogue A scoping paper for The Forests Dialogue" (PDF). The Forest Dialogue. Retrieved 16 January 2014.
  7. Walter, C. (2010). "The 20-year environmental safety record of GM trees". Nature Biotechnology. 28 (7): 656–658. doi:10.1038/nbt0710-656. PMID 20622831.
  8. "COP 8 Decision VIII/19 Forest biological diversity: implementation of the programme of work". Convention on Biological Diversity. Retrieved 16 January 2014.
  9. Sedjo, R.A. (2004). "Genetically Engineered Trees: Promise and Concerns" (PDF). Resources for the Future: 20–21. Archived from the original (PDF) on 2012-05-12. Retrieved 2013-11-15.
  10. Bradshaw, A.H. (2001). "Plotting a course for GM forestry". Nature Biotechnology. 19 (12): 1103–1104. doi:10.1038/nbt1201-1103b. PMID 11731771.
  11. Strauss, S.H. (2009). "Strangled at birth? Forest biotech and the Convention on Biological Diversity". Nature Biotechnology. 27 (6): 519–27. doi:10.1038/nbt0609-519. PMID 19513052.
  12. Williams, C.G. (2010). "Long-distance pine pollen still germinates after meso-scale dispersal". American Journal of Botany. 97 (5): 846–855. doi:10.3732/ajb.0900255. PMID 21622450.
  13. Kuparinen, A. (2008). "Assessing the risk of gene flow from genetically modified trees carrying mitigation transgenes". Biological Invasions. 10 (3): 282. doi:10.1007/s10530-007-9129-6.
  14. James, R.R. (1997). "Utilizing a social ethic toward the environment in assessing genetically engineered insect-resistance in trees". Agriculture and Human Values. 14 (3): 237–249. doi:10.1023/A:1007408811726.
  15. Ahuja, M.R. (2011). "Fate of transgenes in the forest tree genome". Tree Genetics & Genomes. 7 (2): 226. doi:10.1007/s11295-010-0339-1.
  16. "USDA Weighs Plan to Bring GM Eucalyptus to Southeast Pinelands". New York Times. January 29, 2010.
  17. Genetically modified low-lignin eucalyptus yields twice the sugar
  18. Poplars “designed for deconstruction” a major boon to biofuels
  19. Researchers design trees that make it easier to produce pulp
  20. Sedjo, R.A. (2004). "Genetically Engineered Trees: Promise and Concerns" (PDF). Resources for the Future: 15. Archived from the original (PDF) on 2012-05-12. Retrieved 2013-11-15.
  21. Owusu, R.A. (1999). "GM technology in the forest sector - A scoping study for WWF" (PDF). WWF: 10.
  22. Nottingham, S. (2002). Genescapes - The Ecology of Genetic Engineering. Zed Books. ISBN 978-1842770375.
  23. Doering, D. S. (2001). "Will the Marketplace See the Sustainable Forest for the Transgenic Trees?" (PDF). Proceedings of the First International Symposium on Ecological and Societal Aspects of Transgenic Plantations: 70–81. Archived from the original (PDF) on 2014-02-02. Retrieved 2014-01-25. The communities at or near the plantations and the paper mills may receive a net environmental benefit of cleaner water and air in their communities. (p. 73)
  24. Meilan, R. (2007). "Manipulating Lignin Biosynthesis to Improve Populus as a Bio-Energy Feedstock" (PDF). Institute of Forest Biotechnology, Genetically Engineered Forest Trees - Identifying Priorities for Ecological Risk Assessment: 55–61. Some scientists believe ... that reducing lignin content may lead to increases in cellulose content. But critics argue that reductions in lignin will compromise the structural integrity of the plant and make it more susceptible to pathogens, and diseases. (p. 59)
  25. Hall, C. (2007). "GM technology in forestry: lessons from the GM food 'debate'". International Journal of Biotechnology. 9 (5): 436–447. doi:10.1504/ijbt.2007.014270. Altering the quality or quantity of lignin may have significant impacts on the survival abilities of the tree, such as impairing its pest or disease resistance and necessitating the use of additional pesticides.
  26. Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone
  27. Genetically Modified Trees Could Clean Up Paper Industry
  28. Mathews, J.H.; Campbell, M.M. (2000). "The advantages and disadvantages of the application of genetic engineering to forest trees: a discussion". Forestry. 73 (4): 371–380. doi:10.1093/forestry/73.4.371. As Pullman et al.(1998) pointed out, modification of trees’ adaptation to environmental stresses will enable foresters to grow more desirable commercial tree species on a broader range of soil types and planting sites. (p.375)
  29. Harfouche, A.; et al. (2011). "Tree genetic engineering and applications to sustainable forestry and biomass production". Trends in Biotechnology. 29 (1): 9–17. doi:10.1016/j.tibtech.2010.09.003. PMID 20970211. ArborGen is a joint venture between International Paper Company (USA) MeadWestvaco (USA) and Rubicon Limited (New Zealand) (p.13)
  30. Institute of Forest Biotechnology (2007). "Genetically Engineered Forest Trees - Identifying Priorities for Ecological Risk Assessment - Summary of a Multistakeholder Workshop" (PDF). private company ArborGen is reportedly focusing on the development of three GE varieties: fast-growing loblolly pine for Southern pine plantations, low-lignin eucalyptus for use in South America, and cold-hardy eucalyptus for the Southern U.S. (p. ix) Cite journal requires |journal= (help)
  31. "Deliberate release of genetically modified trees An abundance of poplars". GMO Safety. June 1, 2012. A gene has been introduced into the trees that makes them less sensitive to cold. Until now cultivation of eucalyptus in the US was only possible on the southern tip of Florida; frost tolerance could mean that cultivation would be possible in other parts of the USA.
  32. Knäbel M, Friend AP, Palmer JW, Diack R, Wiedow C, Alspach P, Deng C, Gardiner SE, Tustin DS, Schaffer R, Foster T, Chagné D (2015). "Genetic control of pear rootstock-induced dwarfing and precocity is linked to a chromosomal region syntenic to the apple Dw1 loci". BMC Plant Biol. 15: 230. doi:10.1186/s12870-015-0620-4. PMC 4580296. PMID 26394845.
  33. Foster TM, McAtee PA, Waite CN, Boldingh HL, McGhie TK (2017). "Apple dwarfing rootstocks exhibit an imbalance in carbohydrate allocation and reduced cell growth and metabolism". Hortic Res. 4: 17009. doi:10.1038/hortres.2017.9. PMC 5381684. PMID 28435686.
  34. Overbeek W. (2012). "An overview of industrial tree plantation conflicts in the global South. Conflicts, trends, and resistance struggles" (PDF). EJOLT. 3: 84.
  35. Vidal, J. (November 15, 2012). "The GM tree plantations bred to satisfy the world's energy needs - Israeli biotech firm says its modified eucalyptus trees can displace the fossil fuel industry". Guardian.
  36. Gerber, J.F. (2011). "Conflicts over industrial tree plantations in the South: Who, how and why?". Global Environmental Change. 21: 165–176. doi:10.1016/j.gloenvcha.2010.09.005. Fast-wood plantations tend to destabilize water cycles provoking reduced water flow throughout the year, the disappearance of streams during the dry season, and damages to other (agro-)ecosystems (p.167)
  37. Owusu, R.A. (1999). "GM technology in the forest sector - A scoping study for WWF" (PDF). WWF. Biotechnology may inadvertently become yet another driver for inappropriate plantation development. Increased soil nutrient and water demand of fast growing species on short rotations could lead to irrecoverable loss of site productivity. (p.5)
  38. Nottingham, S. (2002). Genescapes - The Ecology of Genetic Engineering. Zed Books. ISBN 9781842770375. fast-growing transgenic trees will make additional demands on soil nutrients and water, with consequences for the long-term fertility of soils. Substantial fertilizer inputs might be necessary to maintain high yields
  39. Gene manipulation boosts tree growth rate and size
  40. "Into the Wildwood". The Economist. May 4, 2013.
  41. Harfouche, A. (2011). "Tree genetic engineering and applications to sustainable forestry and biomass production". Trends in Biotechnology. 29 (1): 13. doi:10.1016/j.tibtech.2010.09.003. PMID 20970211.
  42. Powell, William (March 2014) "the American Chestnut's Genetic Rebirth", Scientific American, Volume 310, Number 3, Page 52
  43. Lang, Chris (2004). "China: Genetically modified madness". The World Rainforest Movement. Archived from the original on 3 February 2014. Retrieved 29 January 2014. Two years ago, China's State Forestry Administration approved genetically modified (GM) poplar trees for commercial planting.
  44. Then, C.; Hamberger, S. (2010). "Genetically engineered trees – a ticking "time bomb"?" (PDF). Testbiotech.de.
  45. Sedjo, R.A. (2005). "Will Developing Countries be the Early Adopters of Genetically Engineered Forests? Resources for the Future" (PDF). AgBioForum. 8 (4): 205–211. the engineered gene has probably spread beyond the area of the original plantings (p.206)
  46. Carman, N. (2006). "Ecological and Social Impacts of Fast Growing Timber Plantations and Genetically Engineered Trees" (PDF). Dogwood Alliance. The Nanjing Institute of Environmental Science has reported that contamination of native poplars with the Bt gene is already occurring. (p.4)
  47. Then, C.; Hamberger, S. (2010). "Genetically engineered trees – a ticking "time bomb"?" (PDF). Testbiotech. Bt poplars are grown alongside non-transgenic trees, possibly delaying the emergence of resistances. If this is the case, the transgenic poplars will have a higher fitness in comparison to the other trees, thus conceivably fostering their invasiveness in the mid or even long term. (p.16)
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