Living building material

A living building material (LBM) is a material used in construction or industrial design that behaves in a way resembling a living organism. Examples include: self-mending biocement,[1] self-replicating concrete replacement,[2] and mycelium-based composites for construction and packaging.[3][4] Artistic projects include building components and household items.[5][6][7][8]

History

The development of living building materials began with research of methods for mineralizing concrete, that were inspired by coral mineralization. The use of microbiologically induced calcite precipitation (MICP) in concrete was pioneered by Adolphe et al. in 1990, as a method of applying a protective coating to building facades.[9]

In 2007, "Greensulate", a mycelium-based building insulation material was introduced by Ecovative Design, a spin off of research conducted at the Rensselaer Polytechnic Institute.[10][11] Mycelium composites were later developed for packaging, sound absorption, and structural building materials such as bricks.[12][13][14]

In the United Kingdom, the Materials for Life (M4L) project was founded at Cardiff University in 2013 to "create a built environment and infrastructure which is a sustainable and resilient system comprising materials and structures that continually monitor, regulate, adapt and repair themselves without the need for external intervention."[15] M4L led to the UK's first self-healing concrete trials.[16] In 2017 the project expanded into a consortium led by the universities of Cardiff, Cambridge, Bath and Bradford, changing its name to Resilient Materials 4 Life (RM4L) and receiving funding from the Engineering and Physical Sciences Research Council.[16] This consortium focuses on four aspects of material engineering: self-healing of cracks at multiple scales; self-healing of time-dependent and cycling loading damage; self-diagnosis and healing of chemical damage; and self-diagnosis and immunization against physical damage.[17]

In 2016 the United States Department of Defense's Defense Advanced Research Projects Agency (DARPA) launched the Engineered Living Materials (ELM) program.[18] The goal of this program is to "develop design tools and methods that enable the engineering of structural features into cellular systems that function as living materials, thereby opening up a new design space for building technology... [and] to validate these new methods through the production of living materials that can reproduce, self-organize, and self-heal."[19] In 2017 the ELM program contracted Ecovative Design to produce "a living hybrid composite building material... [to] genetically re-program that living material with responsive functionality [such as] wound repair... [and to] rapidly reuse and redeploy [the] material into new shapes, forms, and applications."[20] In 2020 a research group at the University of Colorado, funded by an ELM grant, published a paper after successfully creating exponentially regenerating concrete.[2][21][22]

Self-Replicating Concrete
The fracture energy of a living building material compared with two controls: one with no cyanobacteria, and one with no cyanobacteria and a high pH.[2]

Synthesis and Fabrication

Self-replicating concrete is composed of a sand and hydrogel scaffold that houses synechococcus bacteria. The sand and hydrogel combination has a lower pH, lower ionic strength, and lower curing temperatures than a typical concrete mix, allowing it to serve as a growth medium for the bacteria. As the bacteria reproduce they spread through the scaffold, and biomineralize it with calcium carbonate, which is the main contributor to the overall strength and durability of the material. After mineralization the sand-hydrogel compound is strong enough to be used in construction, as concrete or mortar.[2]

The bacteria in self-replicating concrete react to humidity changes: they are most active - and reproduce the fastest - in an environment with 100% humidity, though a drop to 50% does not have a large impact on the cellular activity. Lower humidity does result in a stronger material than high humidity.[2]

As the bacteria reproduce, their biomineralization activity increases; this allows production capacity to scale exponentially.[2]

Properties

The structural properties of this material are similar to those of Portland cement-based mortars: it has an elastic modulus of 293.9 MPa, and a tensile strength of 3.6 MPa (the minimum required value for Portland-cement based concrete is approximately 3.5 MPa);[2] however it has a fracture energy of 170 N, which is much less than most standard concrete formulations, which can reach up to several kN.

Uses

Self-replicating concrete can be used in a variety of applications and environments, but the effect of humidity on the properties of the end material (see above) means that the application of the material must be tailored to its environment. In humid environments the material can be used as to fill cracks in roads, walls and sidewalks, sipping into cavities and growing into a solid mass as it sets;[23] while in drier environments it can be used structurally, due to its increased strength in low-humidity environments.

Unlike traditional concrete, the production of which releases massive amounts of carbon dioxide to the atmosphere, the bacteria used in self-replicating concrete absorb carbon dioxide, resulting in a lower carbon footprint.[24]

This self-replicating concrete is not meant to replace standard concrete, but to create a new class of materials, with a mixture of strength, ecological benefits, and biological functionality.[25]

Self-Mending Biocement
Biocement application in bee nesting. Figure (a) shows a virtual diagram of the biocement brick and housing area for bees. Figure (b) shows the cross section of the design and the holes the bees can nest in. Figure (c) shows the prototype of the bee block made of biocement.[26]

Definition

Advancements in optimizing methods to use microorganisms to facilitate carbonate precipitation are rapidly developing.[27] Biocement specifically is a material that is most well known for its self-healing properties due to microscopic organisms such as bacteria and fungi that are used along with calcium carbonate (CaCO3) in the formation process of the material.[27][26]

Synthesis and Fabrication

Microscopic organisms are the key component in the formation of bioconcrete as they provide the nucleation site for CaCO3 to precipitate on the surface.[26] Microorganisms such as Sporosarcina pasteurii are useful in these fabrications as they create alkaline environments where high is pH and dissolved inorganic carbon(DIC) count are both high.[28] These factors are essential for micro biologically induced calcite precipitation(MICP) which is the main mechanism in which bioconcrete is formed.[27][26][28][29] Other organisms that can be used to induce this process are photosynthetic microorganisms such as microalgae and cyanobacteria, or sulphate reducing bacteria(SRB) such as Desulfovibrio desulfuricans.[27][30] The nucleation of calcium carbonate is dependent on four major factors: 1. Calcium concentration, 2. DIC concentration, 3. pH level, and 4. availability of nucleation sites. As long as calcium ion concentration is high enough, the microorganisms previously described can create such an environment through processes such as ureolysis.[27][31]

Properties

Biocement is able to "self-heal" due to bacteria, calcium lactate, nitrogen, and phosphorus components that are mixed into the material.[32] These components have the ability to remain active in biocement for up to 200 years. Biocement like any other concrete can crack due to external forces and stresses. Unlike normal concrete however, the microorganisms in biocement can germinate when introduced to water.[33] Rain can supply this water which is an environment that biocement would find itself in. Once introduced to water, the bacteria will activate and feed on the calcium lactate that was part of the mixture.[33] This feeding process also consumes oxygen which converts the originally water soluble calcium lactate into insoluble limestone. This limestone then solidifies on surface it is lying on, which in this case is the cracked area, thereby sealing the crack up.[33]

Oxygen is one of the main elements that cause corrosion in materials such as metals. When biocement is used in steel reinforced concrete structures, the microorganisms consume the oxygen thereby increasing corrosion resistance.This property also allows for water resistance as it actually induces healing, and reducing overall corrosion.[33] Water concrete aggregates are what are used to prevent corrosion and these also have the ability to be recycled.[33] There are different methods to form these such as through crushing or grinding of the biocement.[27]

The permeability of biocement is also higher compared to normal cement.[26] This is due to the higher porosity of biocement and this can leader to larger crack propagation when exposed to strong enough forces. The fact that biocement is now roughly 20% composed of a self healing agent also decreases its mechanical strength.[26][34] The mechanical strength of bioconcrete is about 25% weaker than normal concrete, making its compressive strength significantly lower.[34] There are also some organisms such as Pesudomonas aeruginosa that are effective in creating biocement but are unsafe to be near humans so these must be avoided.[35]

Uses

Biocement is currently used in applications such as in sidewalks and pavements in buildings.[36] There are ideas of biological building constructions as well. The uses of biocement are still not widespread because there is currently not a feasible method of mass producing biocement to such a high extent.[37] There is also much more definitive testing that needs to be done to confidently use biocement in such large scale applications where mechanical strength can not be compromised. The cost of biocement is also twice as much as normal concrete.[38] Different uses in smaller applications however include spray bars, hoses, drop lines, and bee nesting. Biocement is still in its developmental stages however its potential proves promising for its future uses.

Mycelium-Based Composites
One of the examples of the structure of a mycelium based composites.[39]

Mycelium composites are products that uses mycelium, which is one of the main components of a fungus. Fungus depends on mycelium to get nutrients from the environment. There are several uses of mycelium composites in the industry because it is economically and environmentally sustainable. There are several ways to fabricate and synthesize mycelium composites which can alter the properties to produce different types of materials for different types of uses.

Synthesis and fabrication

Mycelium- Based Composites are usually synthesized by using different kinds of fungus, especially mushroom.[40] An individual microbe of fungi is introduced to different types of organic substances to form a composite.[41] The selection of fungal species is important for creating a product with specific properties. Some of the fungal species that are used to make composites are G. lucidum, Ganoderma sp. P. ostretus, Pleurotus sp., T. versicolor, Trametes sp., etc.[42] A dense network is formed when the mycelium of the microbe of fungi degrades and colonizes the organic substance. Plant waste is a common organic substrate that is used in mycelium based composites. Fungal mycelium is incubated with a plants waste product to produce sustainable alternatives mostly for petroleum based materials.[42][3] The mycelium and organic substrate needed to incubate properly and this time is crucial as it is the period that these particles interact together and bind into one to form a dense network and hence form a composite. During this incubation period, mycelium uses the essential nutrients such as carbon, minerals, and water from the waste plant product.[41] Some of the organic substrate components include cotton, wheat grain, rice husk, sorghum fiber, agricultural waste, sawdust, bread particles, banana peel, coffee residue, etc.[42]  The composites are synthesized and fabricated using different techniques such as adding carbohydrates, altering fermentation conditions, using different fabrication technology, altering post- processing stages, and modifying genetics or biochemicals to form products with certain properties.[40] Fabrication of most of the mycelium composites are by using plastic molds, so the mycelium can be grown directly into the desired shape.[41][42]  Other fabrication methods include laminate skin mold, vacuum skin mold, glass mold, plywood mold, wooden mold, petri dish mold, tile mold, etc.[42] During fabrication process, it is essential to have sterilized environment, a controlled environment condition of light, temperature (25-35°C) and humidity around 60-65% for the best results.[41] One way to synthesize a mycelium based composite is by mixing different composition ratio of fibers, water and mycelium together and putting in a PVC molds in layers while compressing each layer and letting it incubate for couple of days.[43] Mycelium based composites can be processed in foam, laminate and mycelium sheet by using processing techniques such as laster cutting, cold and heat compression, etc.[41][42] Mycelium composites tend to absorb water when they are newly fabricated, therefore this property can be changed by oven drying the product.[42]

Properties

One of the advantages about using mycelium based composites is that properties can be altered depending on fabrication process and the use of different fungus. Properties depend on type of fungus used and where they are grown.[42] Additionally, fungi has an ability to degrade the cellulose component of the plant to make composites in a preferable manner.[3] Some important mechanical properties such as compressive strength, morphology, tensile strength, hydrophobicity, and flexural strength can be modified as well for different use of the composite.[42] To increase the tensile strength, the composite can go through heat pressing.[40] A mycelium composite made out of 75 wt% rice hulls have density of 193 kg/m3, while 75 wt% wheat grains has 359 kg/m3, which shows how different mycelium substance has effect on its property.[3] One the methods to increase the density of the composite would be by deleting a hydrophobin gene.[42] These composites also have the ability of self fusion which increases their strength.[42] Mycelium based composites are usually compact, porous, lightweight and a good insulator. The main property of these composites is that they are entirely natural, therefore sustainable. Other advantage of mycelium based composites is that this substance acts as an insulator, is fireproof, nontoxic, water resistance, rapidly growing, and has an ability to bond with neighboring mycelium products.[44] Mycelium based foams (MBFs) and sandwich components are two common type of the composites.[3] MBFs are the most efficient type because of their low density property, high quality, and sustainability.[39] The density of MBFs can be decreased by using substrates that are smaller than 2mm in diameter.[39] These composites have higher thermal conductivity as well.[39]

Uses

One of the most common use of mycelium based composites is for the alternatives for petroleum and polystyrene based materials.[42] These synthetic foams are usually used for sustainable design and architecture products. The use of mycelium based composites are based on their properties. There are several bio-sustainable companies such as Ecovative Design LLC, MycoWorks, MyCoPlast, etc. that use mycelium based composites that make protective packaging for electronics and food, bricks, leather substitutes, alternatives for floors and acoustic tiles, thermal and acoustic insulation, construction panels, etc.[42] The property of being able to bond with neighboring composite helps the mycelium based composite to form strong bonds for a brick which are widely used.[44] There is a 40 foot tall tower in MoMa PS1 at New York City, Hy-Fi made using 1000 bricks made from corn stalks and mycelium.[45] This product won the annual Young Architects Program (YAP) contest in 2014.[46] There are also other several commonly used products such as lamps, kitchen utensils, ceiling panels, decorative items, fashion items, chair, etc made out of mycelium.[44] In architecture, mycelium based composites are widely used because they have better insulation performance and fire resistance than currently used products.[42] Mycelium is being used more in industry to replace common plastic materials that are harming the environment. These products are manufactured using low energy, natural manufacturing process and are biodegradable.[47]

Further applications

Beyond the use of living building materials, the application of microbially induced calcium carbonate precipitation (MICP) has the possibility of helping remove pollutants from wastewater, soil, and the air. Currently, heavy metals and radionuclei provide a challenge to remove from water sources and soil. Radionuclei in ground water do not respond to traditional methods of pumping and treating the water, and for heavy metals contaminating soil, the methods of removal include phytoremediation and chemical leaching do work; however, these treatments are expensive, lack longevity in effectiveness, and can destroy the productivity of the soil for future uses.[48] By using ureolytic bacteria that is capable of CaCO3 precipitation, the pollutants can move into the calcite structure, thereby removing them from the soil or water. This works through substitution of calcium ions for pollutants that then form solid particles and can be removed.[48] It's reported that 95% of these solid particles can be removed by using ureolytic bacteria.[48] However, when calcium scaling in pipelines occurs, MICP cannot be used as it is calcium-based. Instead of calcium, it is possible to add a low concentration of urea to remove up to 90% of the calcium ions.[48]

Another further application involves a self-constructed foundation that forms in response to pressure through the use of engineering bacteria. The engineered bacteria could be used to detect increased pressure in soil, and then cement the soil particles in place, effectively solidifying the soil.[1] Within soil, pore pressure consists of two factors: the amount of applied stress, and how quickly water in the soil is able to drain. Through analyzing the biological behavior of the bacteria in response to a load and the mechanical behavior of the soil, a computational model can be created.[1] With this model, certain genes within the bacteria can be identified and modified to respond a certain way to a certain pressure. However, the bacteria analyzed in this study was grown in a highly-controlled lab, so real soil environments may not be as ideal.[1] This is a limitation of the model and study it originated from, but it still remains a possible application of living building materials.

References
  1. Dade-Robertson, Martyn; Mitrani, Helen; Corral, Javier Rodriguez; Zhang, Meng; Hernan, Luis; Guyet, Aurelie; Wipat, Anil (2018-05-24). "Design and modelling of an engineered bacteria-based, pressure-sensitive soil". Bioinspiration & Biomimetics. 13 (4): 046004. Bibcode:2018BiBi...13d6004D. doi:10.1088/1748-3190/aabe15. ISSN 1748-3190. PMID 29652250.
  2. Heveran, Chelsea M.; Williams, Sarah L.; Qiu, Jishen; Artier, Juliana; Hubler, Mija H.; Cook, Sherri M.; Cameron, Jeffrey C.; Srubar, Wil V. (2020-01-15). "Biomineralization and Successive Regeneration of Engineered Living Building Materials". Matter. 0 (2): 481–494. doi:10.1016/j.matt.2019.11.016. ISSN 2590-2393.
  3. Jones, Mitchell; Bhat, Tanmay; Huynh, Tien; Kandare, Everson; Yuen, Richard; Wang, Chun H.; John, Sabu (2018). "Waste-derived low-cost mycelium composite construction materials with improved fire safety". Fire and Materials. 42 (7): 816–825. doi:10.1002/fam.2637. ISSN 1099-1018.
  4. Abhijith, R.; Ashok, Anagha; Rejeesh, C. R. (2018-01-01). "Sustainable packaging applications from mycelium to substitute polystyrene: a review". Materials Today: Proceedings. Second International Conference on Materials Science (ICMS2017) during 16 – 18 February 2017. 5 (1, Part 2): 2139–2145. doi:10.1016/j.matpr.2017.09.211. ISSN 2214-7853.
  5. Boyer, Mark (2014-06-25). "Philip Ross Molds Fast-Growing Fungi Into Mushroom Building Bricks That Are Stronger than Concrete". inhabitat. Retrieved 2020-01-18.
  6. "Building with Mushrooms". Critical Concrete. 2018-04-23. Retrieved 2020-01-18.
  7. "Pavilion grown from mycelium acts as pop-up performance space". Dezeen. 2019-10-29. Retrieved 2020-01-18.
  8. Hitti, Natashah (2019-01-07). "Nir Meiri makes sustainable lamp shades from mushroom mycelium". Dezeen. Retrieved 2020-01-18.
  9. EP 0388304B1, Adolphe, Jean Pierre & Loubiere, "Procédé de traitement biologique d'une surface artificielle", published 1990-09-19, issued 1994-09-28, assigned to Universite Pierre et Marie Curie
  10. US 9485917B2, Bayer & McIntyre, "Method for producing grown materials and products made thereby", published 2008-06-19, issued 216-11-08, assigned to Ecovative Design LLC
  11. Pasko, Jessica (25 June 2007). "Mushrooms are eco-friendly insulation". USA Today. Retrieved 2 April 2020.
  12. Holt, G. A.; Mcintyre, G.; Flagg, D.; Bayer, E.; Wanjura, J. D.; Pelletier, M. G. (2012-08-01). "Fungal Mycelium and Cotton Plant Materials in the Manufacture of Biodegradable Molded Packaging Material: Evaluation Study of Select Blends of Cotton Byproducts". Journal of Biobased Materials and Bioenergy. 6 (4): 431–439. doi:10.1166/jbmb.2012.1241. ISSN 1556-6560.
  13. Pelletier, M.G.; Holt, G.A.; Wanjura, J.D.; Bayer, E.; McIntyre, G. (November 2013). "An evaluation study of mycelium based acoustic absorbers grown on agricultural by-product substrates". Industrial Crops and Products. 51: 480–485. doi:10.1016/j.indcrop.2013.09.008. ISSN 0926-6690.
  14. Jones, Mitchell; Huynh, Tien; Dekiwadia, Chaitali; Daver, Fugen; John, Sabu (2017-08-01). "Mycelium Composites: A Review of Engineering Characteristics and Growth Kinetics". Journal of Bionanoscience. 11 (4): 241–257. doi:10.1166/jbns.2017.1440. ISSN 1557-7910.
  15. "Materials for Life (M4L)". Cardiff University. Retrieved 2020-03-30.
  16. "Boost for ground-breaking research into self-healing construction materials - EPSRC website". epsrc.ukri.org. Retrieved 2020-03-30.
  17. "Research Themes". M4L. Retrieved 2020-04-02.
  18. "Living Structural Materials Could Open New Horizons for Engineers and Architects". www.darpa.mil. Retrieved 2020-03-30.
  19. "Engineered Living Materials". www.darpa.mil. Retrieved 2020-03-30.
  20. "Ecovative Design Awarded $9.1 Million U.S. Department of Defense Research Contract to Develop & Scale a New Generation of Living Building Materials". GROW.bio. Retrieved 2020-03-30.
  21. Timmer, John (2020-01-18). ""Living concrete" is an interesting first step". Ars Technica. Retrieved 2020-04-02.
  22. "Environmentally friendly 'living concrete' capable of self-healing". BBC Science Focus Magazine. Retrieved 2020-04-02.
  23. Kubrick, Kaitlyn (2020-01-16). "Scientists Produced Self-Replicating Materials". Somag News. Retrieved 2020-04-23.
  24. Rodgers, Lucy (2018-12-17). "The massive CO2 emitter you may not know about". BBC News. Retrieved 2020-04-23.
  25. Wilson, Mark (2020-01-27). "These DARPA-funded bricks can self-repair—and replicate". Fast Company. Retrieved 2020-04-23.
  26. Lee, Chungmin; Lee, Hyesun; Kim, Ok Bin (November 2018). "Biocement Fabrication and Design Application for a Sustainable Urban Area". Sustainability. 10 (11): 4079. doi:10.3390/su10114079.
  27. Irfan, M. F.; Hossain, S. M. Z.; Khalid, H.; Sadaf, F.; Al-Thawadi, S.; Alshater, A.; Hossain, M. M.; Razzak, S. A. (2019-09-01). "Optimization of bio-cement production from cement kiln dust using microalgae". Biotechnology Reports. 23: e00356. doi:10.1016/j.btre.2019.e00356. ISSN 2215-017X. PMC 6609786. PMID 31312609.
  28. Seifan, Mostafa; Samani, Ali Khajeh; Berenjian, Aydin (2016-03-01). "Bioconcrete: next generation of self-healing concrete". Applied Microbiology and Biotechnology. 100 (6): 2591–2602. doi:10.1007/s00253-016-7316-z. hdl:10289/11244. ISSN 1432-0614. PMID 26825821.
  29. Wiesmann, U. N.; DiDonato, S.; Herschkowitz, N. N. (1975-10-27). "Effect of chloroquine on cultured fibroblasts: release of lysosomal hydrolases and inhibition of their uptake". Biochemical and Biophysical Research Communications. 66 (4): 1338–1343. doi:10.1016/0006-291x(75)90506-9. ISSN 1090-2104. PMID 4.
  30. Hagiya, Hideharu; Kimura, Keigo; Nishi, Isao; Yamamoto, Norihisa; Yoshida, Hisao; Akeda, Yukihiro; Tomono, Kazunori (2018-02-01). "Desulfovibrio desulfuricans bacteremia: A case report and literature review". Anaerobe. 49: 112–115. doi:10.1016/j.anaerobe.2017.12.013. ISSN 1075-9964. PMID 29305996.
  31. Wu, Jun; Wang, Xian-Bin; Wang, Hou-Feng; Zeng, Raymond J. (2017-07-24). "Microbially induced calcium carbonate precipitation driven by ureolysis to enhance oil recovery". RSC Advances. 7 (59): 37382–37391. doi:10.1039/C7RA05748B. ISSN 2046-2069.
  32. Stabnikov, V.; Ivanov, V. (2016-01-01), Pacheco-Torgal, Fernando; Ivanov, Volodymyr; Karak, Niranjan; Jonkers, Henk (eds.), "3 - Biotechnological production of biopolymers and admixtures for eco-efficient construction materials", Biopolymers and Biotech Admixtures for Eco-Efficient Construction Materials, Woodhead Publishing, pp. 37–56, ISBN 978-0-08-100214-8, retrieved 2020-04-16
  33. "Articles - Self-Healing Concrete". www.ingenia.org.uk. Retrieved 2020-04-16.
  34. Stabnikov, V.; Ivanov, V. (2016-01-01), Pacheco-Torgal, Fernando; Ivanov, Volodymyr; Karak, Niranjan; Jonkers, Henk (eds.), "3 - Biotechnological production of biopolymers and admixtures for eco-efficient construction materials", Biopolymers and Biotech Admixtures for Eco-Efficient Construction Materials, Woodhead Publishing, pp. 37–56, ISBN 978-0-08-100214-8, retrieved 2020-04-16
  35. Dhami, Navdeep K.; Alsubhi, Walaa R.; Watkin, Elizabeth; Mukherjee, Abhijit (2017-07-11). "Bacterial Community Dynamics and Biocement Formation during Stimulation and Augmentation: Implications for Soil Consolidation". Frontiers in Microbiology. 8: 1267. doi:10.3389/fmicb.2017.01267. ISSN 1664-302X. PMC 5504299. PMID 28744265.
  36. Stewart, Andrew. "The 'living concrete' that can heal itself". CNN. Retrieved 2020-04-16.
  37. "Bioconcrete: The Construction Phenomenon". Cobalt Recruitment. Retrieved 2020-04-16.
  38. Iezzi, Brian; Brady, Richard; Sardag, Selim; Eu, Benjamin; Skerlos, Steven (2019-01-01). "Growing bricks: Assessing biocement for lower embodied carbon structures". Procedia CIRP. 26th CIRP Conference on Life Cycle Engineering (LCE) Purdue University, West Lafayette, IN, USA May 7-9, 2019. 80: 470–475. doi:10.1016/j.procir.2019.01.061. ISSN 2212-8271.
  39. Girometta, Carolina; Picco, Anna Maria; Baiguera, Rebecca Michela; Dondi, Daniele; Babbini, Stefano; Cartabia, Marco; Pellegrini, Mirko; Savino, Elena (January 2019). "Physico-Mechanical and Thermodynamic Properties of Mycelium-Based Biocomposites: A Review". Sustainability. 11 (1): 281. doi:10.3390/su11010281.
  40. Appels, Freek V. W.; Camere, Serena; Montalti, Maurizio; Karana, Elvin; Jansen, Kaspar M. B.; Dijksterhuis, Jan; Krijgsheld, Pauline; Wösten, Han A. B. (2019-01-05). "Fabrication factors influencing mechanical, moisture- and water-related properties of mycelium-based composites". Materials & Design. 161: 64–71. doi:10.1016/j.matdes.2018.11.027. ISSN 0264-1275.
  41. "When the Material Grows: A Case Study on Designing (with) Mycelium-based Materials". International Journal of Dsign. Retrieved 2020-04-16.
  42. Attias, Noam; Danai, Ofer; Abitbol, Tiffany; Tarazi, Ezri; Ezov, Nirit; Pereman, Idan; Grobman, Yasha J. (2020-02-10). "Mycelium bio-composites in industrial design and architecture: Comparative review and experimental analysis". Journal of Cleaner Production. 246: 119037. doi:10.1016/j.jclepro.2019.119037. ISSN 0959-6526.
  43. Elsacker, Elise; Vandelook, Simon; Brancart, Joost; Peeters, Eveline; Laet, Lars De (2019-07-22). "Mechanical, physical and chemical characterisation of mycelium-based composites with different types of lignocellulosic substrates". PLOS ONE. 14 (7): e0213954. doi:10.1371/journal.pone.0213954. ISSN 1932-6203. PMC 6645453. PMID 31329589.
  44. "Building with Mushrooms". Critical Concrete. 2018-04-23. Retrieved 2020-01-18.
  45. Stinson, Liz (2014-07-08). "A 40-Foot Tower Made of Living Fungus Bricks". Wired. ISSN 1059-1028. Retrieved 2020-04-16.
  46. "Tower of "grown" bio-bricks by The Living opens at MoMA PS1". Dezeen. 2014-07-01. Retrieved 2020-04-16.
  47. Jones, Mitchell; Mautner, Andreas; Luenco, Stefano; Bismarck, Alexander; John, Sabu (2020-02-01). "Engineered mycelium composite construction materials from fungal biorefineries: A critical review". Materials & Design. 187: 108397. doi:10.1016/j.matdes.2019.108397. ISSN 0264-1275.
  48. Dhami, Navdeep K.; Reddy, M. Sudhakara; Mukherjee, Abhijit (2013). "Biomineralization of calcium carbonates and their engineered applications: a review". Frontiers in Microbiology. 4: 314. doi:10.3389/fmicb.2013.00314. PMC 3810791. PMID 24194735.
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