Phosphatidylinositol diacylglycerol-lyase

In enzymology, a phosphatidylinositol diacylglycerol-lyase (EC 4.6.1.13) is an enzyme that catalyzes the chemical reaction

1-phosphatidyl-1D-myo-inositol 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
phosphatidylinositol diacylglycerol-lyase
Identifiers
EC number4.6.1.13
CAS number37288-19-0
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Phosphatidylinositol-specific phospholipase C, image from OPM database

Hence, this enzyme has one substrate, 1-phosphatidyl-1D-myo-inositol, and two products, 1D-myo-inositol 1,2-cyclic phosphate and 1,2-diacyl-sn-glycerol.

This enzyme belongs to the family of lyases, specifically the class of phosphorus-oxygen lyases. The systematic name of this enzyme class is 1-phosphatidyl-1D-myo-inositol 1,2-diacyl-sn-glycerol-lyase (1D-myo-inositol-1,2-cyclic-phosphate-forming). Other names in common use include monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, 1-phosphatidylinositol phosphodiesterase, 1-phosphatidyl-D-myo-inositol inositolphosphohydrolase, (cyclic-phosphate-forming), 1-phosphatidyl-1D-myo-inositol diacylglycerol-lyase, and (1,2-cyclic-phosphate-forming). This enzyme participates in inositol phosphate metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1T6M and 2OR2.

References

    • Allan D, Michell RH (1974). "Phosphatidylinositol cleavage catalysed by the soluble fraction from lymphocytes. Activity at pH5.5 and pH7.0". Biochem. J. 142 (3): 591–7. doi:10.1042/bj1420591. PMC 1168323. PMID 4377210.
    • Friedel RO, Brown JD, Durell (1967). "Monophosphatidyl inositol inositolphosphohydrolase in guinea-pig brain". Biochim. Biophys. Acta. 144 (3): 684–6. doi:10.1016/0005-2760(67)90059-8. PMID 4294905.
    • Irvine RF (1982). "The enzymology of stimulated inositol lipid turnover". Cell Calcium. 3 (4–5): 295–309. doi:10.1016/0143-4160(82)90018-5. PMID 6297738.
    • Michell RH, Allan D (1975). "Inositol cyclis phosphate as a product of phosphatidylinositol breakdown by phospholipase C (Bacillus cereus)". FEBS Lett. 53 (3): 302–4. doi:10.1016/0014-5793(75)80041-X. PMID 236918. S2CID 5813981.
    • Low MG, Finean JB (1977). "Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C". Biochem. J. 167 (1): 281–4. doi:10.1042/bj1670281. PMC 1183647. PMID 588258.
    • Henner DJ, Yang M, Chen E, Hellmiss R, Rodriguez H, Low MG (1988). "Sequence of the Bacillus thuringiensis phosphatidylinositol specific phospholipase C". Nucleic Acids Res. 16 (21): 10383. doi:10.1093/nar/16.21.10383. PMC 338883. PMID 3194218.


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