Evolutionary history of plants

There is no evidence that early land plants of the Silurian and early Devonian had roots, although fossil evidence of rhizoids occurs for several species, such as Horneophyton. The earliest land plants did not have vascular systems for transport of water and nutrients either. Aglaophyton, a rootless vascular plant known from Devonian fossils in the Rhynie chert[36] was the first land plant discovered to have had a symbiotic relationship with fungi [37] which formed arbuscular mycorrhizas, literally "tree-like fungal roots", in a well-defined cylinder of cells (ring in cross section) in the cortex of its stems. The fungi fed on the plant's sugars, in exchange for nutrients generated or extracted from the soil (especially phosphate), to which the plant would otherwise have had no access. Like other rootless land plants of the Silurian and early Devonian Aglaophyton may have relied on arbuscular mycorrhizal fungi for acquisition of water and nutrients from the soil.

The fungi were of the phylum Glomeromycota,[38] a group that probably first appeared 1 billion years ago and still forms arbuscular mycorrhizal associations today with all major land plant groups from bryophytes to pteridophytes, gymnosperms and angiosperms and with more than 80% of vascular plants.[39]

Evidence from DNA sequence analysis indicates that the arbuscular mycorrhizal mutualism arose in the common ancestor of these land plant groups during their transition to land[40] and it may even have been the critical step that enabled them to colonise the land.[41] Appearing as they did before these plants had evolved roots, mycorrhizal fungi would have assisted plants in the acquisition of water and mineral nutrients such as phosphorus, in exchange for organic compounds which they could not synthesize themselves.[39] Such fungi increase the productivity even of simple plants such as liverworts.[42][43]

To photosynthesise, plants must absorb CO
2 from the atmosphere. However, making the tissues available for CO
2 to enter allows water to evaporate, so this comes at a price.[44] Water is lost much faster than CO
2 is absorbed, so plants need to replace it. Early land plants transported water apoplastically, within the porous walls of their cells. Later, they evolved three anatomical features that provided the ability to control the inevitable water loss that accompanied  CO
2 acquisition.  First, a waterproof outer covering or cuticle evolved that reduced water loss. Secondly, variable apertures, the  stomata that could open and close to regulate the amount of water lost by evaporation during CO
2 uptake and thirdly intercellular space between photosynthetic parenchyma cells that allowed improved internal distribution of the CO
2 to the chloroplasts. This three-part system provided improved homoiohydry, the regulation of water content of the tissues, providing a particular advantage when water supply is not constant. [45] The high CO
2 concentrations of the Silurian and early Devonian, when plants were first colonising land, meant that they used water relatively efficiently. As CO
2 was withdrawn from the atmosphere by plants, more water was lost in its capture, and more elegant water acquisition and transport mechanisms evolved.[44] Plants growing upwards into the air needed a system for transporting water from the soil to all the different parts of the above-soil plant, especially to photosynthesising parts. By the end of the Carboniferous, when CO
2 concentrations had been reduced to something approaching that of today, around 17 times more water was lost per unit of CO
2 uptake.[44] However, even in the "easy" early days, water was always at a premium, and had to be transported to parts of the plant from the wet soil to avoid desiccation.[45]

Water can be wicked by capillary action along a fabric with small spaces. In narrow columns of water, such as those within the plant cell walls or in tracheids, when molecules evaporate from one end, they pull the molecules behind them along the channels. Therefore, evaporation alone provides the driving force for water transport in plants.[44]  However, without specialized transport vessels, this cohesion-tension mechanism can cause negative pressures sufficient to collapse water conducting cells, limiting the transport water to no more than a few cm, and therefore limiting the size of the earliest plants.[44]

A banded tube from the Late Silurian/Early Devonian. The bands are difficult to see on this specimen, as an opaque carbonaceous coating conceals much of the tube. Bands are just visible in places on the left half of the image. Scale bar: 20 μm

To be free from the constraints of small size and constant moisture that the parenchymatic transport system inflicted, plants needed a more efficient water transport system.  As plants grew upwards, specialised water transport vascular tissues evolved, first in the form of simple hydroids of the type found in the setae of moss sporophytes. These simple elongated cells were dead and water-filled at maturity, providing a channel for water transport, but their thin, unreinforced walls would collapse under modest water tension, limiting the plant height.  Xylem tracheids, wider cells with lignin-reinforced cell walls that were more resistant to collapse under the tension caused by water stress, occur  in more than one plant group by mid-Silurian, and may have a single evolutionary origin, possibly within the hornworts,[46] uniting all tracheophytes.  Alternatively,  they may have evolved more than once.[44]  Much later, in the Cretaceous, tracheids were followed by vessels in flowering plants.[44] As water transport mechanisms and waterproof cuticles evolved, plants could survive without being continually covered by a film of water. This transition from poikilohydry to homoiohydry opened up new potential for colonisation.[44][45]

The early Devonian pretracheophytes Aglaophyton and Horneophyton have unreinforced water transport tubes with wall structures very similar to moss hydroids, but they grew alongside several species of tracheophytes, such as Rhynia gwynne-vaughanii that had xylem tracheids that were well reinforced by bands of lignin. The earliest macrofossils known to have xylem tracheids are small, mid-Silurian plants of the genus Cooksonia.[47] However, thickened bands on the walls of isolated tube fragments are apparent from the early Silurian onwards.[48]

Plants continued to innovate ways of reducing the resistance to flow within their cells, progressively  increasing the efficiency of their water transport and to increase the resistance of the tracheids to collapse under tension.[49][50] During the early Devonian, maximum tracheid diameter increased with time, but may have plateaued in the zosterophylls by mid-Devonian.[49]  Overall transport rate also depends on the overall cross-sectional area of the xylem bundle itself, and some mid-Devonian plants, such as the Trimerophytes, had much larger steles than their early ancestors.[49] While wider tracheids provided higher rates of water transport, they increased the risk of cavitation, the formation of air bubbles resulting from the breakage of the water column under tension.[44] Small pits in tracheid walls allow water to by-pass a defective tracheid while preventing air bubbles from passing through[44] but at the cost of restricted flow rates. By the Carboniferous, Gymnosperms had developed bordered pits,[51][52] valve-like structures that allow high-conductivity  pits to seal when one side of a tracheid is depressurized.

Tracheids have perforated end walls, which impose a great deal of resistance on water flow,[49] but may have had the advantage of isolating air embolisms caused by cavitation or freezing. Vessels first evolved during the dry, low CO
2 periods of the Late Permian, in the horsetails, ferns and Selaginellales independently, and later appeared in the mid Cretaceous in gnetophytes and angiosperms.[44]  Vessel members are open  tubes with no end walls, and are arranged end to end  to operate as if they were one continuous vessel.[49] Vessels allowed the same cross-sectional area of wood to transport much more water than tracheids. [44] This allowed plants to fill more of their stems with structural fibres and also opened a new niche to vines, which could transport water without being as thick as the tree they grew on.[44] Despite these advantages, tracheid-based wood is a lot lighter, thus cheaper to make, as vessels need to be much more reinforced to avoid cavitation.[44] Once plants had evolved this level of control over water evaporation and water transport, they were truly homoiohydric, able to extract water from their environment through root-like organs rather than relying on a film of surface moisture, enabling them to grow to much greater size[45][44] but as a result of their increased independence from their surroundings, most vascular plants lost their ability to survive desiccation - a costly trait to lose.[44] In early land plants, support was mainly provided by turgor pressure, particularly of the outer layer of cells known as the sterome tracheids, and not by the xylem, which was too small, too weak and in too central a position to provide much structural support.[44]  Plants with secondary xylem that  had appeared by mid-Devonian, such as the Trimerophytes and Progymnosperms had much larger vascular cross sections producing strong woody tissue.

An endodermis may have evolved in the earliest plant roots during the Devonian, but the first fossil evidence for such a structure is Carboniferous.[44] The endodermis in the roots surrounds the water transport tissue and regulates ion exchange between the groundwater and the tissues and prevents unwanted pathogens etc. from entering the water transport system. The endodermis can also provide an upwards pressure, forcing water out of the roots when transpiration is not enough of a driver.

The lycopod Isoetes bears microphylls (leaves with a single vascular trace).

The branching pattern of megaphyll veins may indicate their origin as webbed, dichotomising branches.

Leaf lamina. The megaphyllous leaf architecture arose multiple times in different plant lineages

Leaves are the primary photosynthetic organs of a modern plant. The origin of leaves was almost certainly triggered by falling concentrations of atmospheric CO
2 during the Devonian period, increasing the efficiency with which carbon dioxide could be captured for photosynthesis.[53][54]

Leaves certainly evolved more than once. Based on their structure, they are classified into two types: microphylls, which lack complex venation and may have originated as spiny outgrowths known as enations, and megaphylls, which are large and have complex venation that may have arisen from the modification of groups of branches. It has been proposed that these structures arose independently.[55] Megaphylls, according to Walter Zimmerman's telome theory,[56] have evolved from plants that showed a three-dimensional branching architecture, through three transformations—overtopping, which led to the lateral position typical of leaves, planation, which involved formation of a planar architecture, webbing or fusion, which united the planar branches, thus leading to the formation of a proper leaf lamina. All three steps happened multiple times in the evolution of today's leaves.[57]

It is widely believed that the telome theory is well supported by fossil evidence. However, Wolfgang Hagemann questioned it for morphological and ecological reasons and proposed an alternative theory.[58][59] Whereas according to the telome theory the most primitive land plants have a three-dimensional branching system of radially symmetrical axes (telomes), according to Hagemann's alternative the opposite is proposed: the most primitive land plants that gave rise to vascular plants were flat, thalloid, leaf-like, without axes, somewhat like a liverwort or fern prothallus. Axes such as stems and roots evolved later as new organs. Rolf Sattler proposed an overarching process-oriented view that leaves some limited room for both the telome theory and Hagemann's alternative and in addition takes into consideration the whole continuum between dorsiventral (flat) and radial (cylindrical) structures that can be found in fossil and living land plants.[60][61] This view is supported by research in molecular genetics. Thus, James (2009)[62] concluded that "it is now widely accepted that... radiality [characteristic of axes such as stems] and dorsiventrality [characteristic of leaves] are but extremes of a continuous spectrum. In fact, it is simply the timing of the KNOX gene expression!"

Before the evolution of leaves, plants had the photosynthetic apparatus on the stems. Today's megaphyll leaves probably became commonplace some 360mya, about 40my after the simple leafless plants had colonized the land in the Early Devonian. This spread has been linked to the fall in the atmospheric carbon dioxide concentrations in the Late Paleozoic era associated with a rise in density of stomata on leaf surface.[53] This would have resulted in greater transpiration rates and gas exchange, but especially at high CO
2 concentrations, large leaves with fewer stomata would have heated to lethal temperatures in full sunlight. Increasing the stomatal density allowed for a better-cooled leaf, thus making its spread feasible, but increased CO2 uptake at the expense of decreased water use efficiency.[54][63]

The rhyniophytes of the Rhynie chert consisted of nothing more than slender, unornamented axes. The early to middle Devonian trimerophytes may be considered leafy. This group of vascular plants are recognisable by their masses of terminal sporangia, which adorn the ends of axes which may bifurcate or trifurcate.[6] Some organisms, such as Psilophyton, bore enations. These are small, spiny outgrowths of the stem, lacking their own vascular supply.

The zosterophylls were already important in the late Silurian, much earlier than any rhyniophytes of comparable complexity.[64] This group, recognisable by their kidney-shaped sporangia which grew on short lateral branches close to the main axes, sometimes branched in a distinctive H-shape.[6] Many zosterophylls bore pronounced spines on their axes but none of these had a vascular trace. The first evidence of vascularised enations occurs in a fossil clubmoss known as Baragwanathia that had already appeared in the fossil record in the Late Silurian.[65] In this organism, these leaf traces continue into the leaf to form their mid-vein.[66] One theory, the "enation theory", holds that the microphyllous leaves of clubmosses developed by outgrowths of the protostele connecting with existing enations[6] The leaves of the Rhynie genus Asteroxylon, which was preserved in the Rhynie chert almost 20 Million years later than Baragwanathia had a primitive vascular supply – in the form of leaf traces departing from the central protostele towards each individual "leaf".[67] Asteroxylon and Baragwanathia are widely regarded as primitive lycopods,[6] a group still extant today, represented by the quillworts, the spikemosses and the club mosses. Lycopods bear distinctive microphylls, defined as leaves with a single vascular trace. Microphylls could grow to some size, those of Lepidodendrales reaching over a meter in length, but almost all just bear the one vascular bundle. An exception is the rare branching in some Selaginella species.

The more familiar leaves, megaphylls, are thought to have originated four times independently, in the ferns, horsetails, progymnosperms and seed plants.[68] They appear to have originated by modifying dichotomising branches, which first overlapped (or "overtopped") one another, became flattened or planated and eventually developed "webbing" and evolved gradually into more leaf-like structures.[66] Megaphylls, by Zimmerman's telome theory, are composed of a group of webbed branches[66] and hence the "leaf gap" left where the leaf's vascular bundle leaves that of the main branch resembles two axes splitting.[66] In each of the four groups to evolve megaphylls, their leaves first evolved during the Late Devonian to Early Carboniferous, diversifying rapidly until the designs settled down in the mid Carboniferous.[68]

The cessation of further diversification can be attributed to developmental constraints,[68] but why did it take so long for leaves to evolve in the first place? Plants had been on the land for at least 50 million years before megaphylls became significant. However, small, rare mesophylls are known from the early Devonian genus Eophyllophyton – so development could not have been a barrier to their appearance.[69] The best explanation so far incorporates observations that atmospheric CO
2 was declining rapidly during this time – falling by around 90% during the Devonian.[70] This required an increase in stomatal density by 100 times to maintain rates of photosynthesis. When stomata open to allow water to evaporate from leaves it has a cooling effect, resulting from the loss of latent heat of evaporation. It appears that the low stomatal density in the early Devonian meant that evaporation and evaporative cooling were limited, and that leaves would have overheated if they grew to any size. The stomatal density could not increase, as the primitive steles and limited root systems would not be able to supply water quickly enough to match the rate of transpiration.[54] Clearly, leaves are not always beneficial, as illustrated by the frequent occurrence of secondary loss of leaves, famously exemplified by cacti and the "whisk fern" Psilotum.

Secondary evolution can also disguise the true evolutionary origin of some leaves. Some genera of ferns display complex leaves which are attached to the pseudostele by an outgrowth of the vascular bundle, leaving no leaf gap.[66] Further, horsetail (Equisetum) leaves bear only a single vein, and appear to be microphyllous; however, both the fossil record and molecular evidence indicate that their forebears bore leaves with complex venation, and the current state is a result of secondary simplification.[71]

Deciduous trees deal with another disadvantage to having leaves. The popular belief that plants shed their leaves when the days get too short is misguided; evergreens prospered in the Arctic circle during the most recent greenhouse earth.[72] The generally accepted reason for shedding leaves during winter is to cope with the weather – the force of wind and weight of snow are much more comfortably weathered without leaves to increase surface area. Seasonal leaf loss has evolved independently several times and is exhibited in the ginkgoales, some pinophyta and certain angiosperms.[73] Leaf loss may also have arisen as a response to pressure from insects; it may have been less costly to lose leaves entirely during the winter or dry season than to continue investing resources in their repair.[74]

Various physical and physiological factors such as light intensity, humidity, temperature, wind speeds etc. have influenced evolution of leaf shape and size. High trees rarely have large leaves, because they are damaged by high winds. Similarly, trees that grow in temperate or taiga regions have pointed leaves, presumably to prevent nucleation of ice onto the leaf surface and reduce water loss due to transpiration. Herbivory, by mammals and insects, has been a driving force in leaf evolution. An example is that plants of the New Zealand genus Aciphylla have spines on their laminas, which probably functioned to discourage the extinct Moas from feeding on them. Other members of Aciphylla, which did not co-exist with the moas, do not have these spines.[75]

At the genetic level, developmental studies have shown that repression of KNOX genes is required for initiation of the leaf primordium. This is brought about by ARP genes, which encode transcription factors. Repression of KNOX genes in leaf primordia seems to be quite conserved, while expression of KNOX genes in leaves produces complex leaves. The ARP function appears to have arisen early in vascular plant evolution, because members of the primitive group Lycophytes also have a functionally similar gene.[76] Other players that have a conserved role in defining leaf primordia are the phytohormones auxin, gibberelin and cytokinin.

The diversity of leaves

The arrangement of leaves or phyllotaxy on the plant body can maximally harvest light and might be expected to be genetically robust. However, in maize, a mutation in only one gene called ABPHYL (ABnormal PHYLlotaxy) is enough to change the phyllotaxy of the leaves, implying that mutational adjustment of a single locus on the genome is enough to generate diversity.[77]

Once the leaf primordial cells are established from the SAM cells, the new axes for leaf growth are defined, among them being the abaxial-adaxial (lower-upper surface) axes. The genes involved in defining this, and the other axes seem to be more or less conserved among higher plants. Proteins of the HD-ZIPIII family have been implicated in defining the adaxial identity. These proteins deviate some cells in the leaf primordium from the default abaxial state, and make them adaxial. In early plants with leaves, the leaves probably just had one type of surface — the abaxial one, the underside of today's leaves. The definition of the adaxial identity occurred some 200 million years after the abaxial identity was established.[78]

How the wide variety of observed plant leaf morphology is generated is a subject of intense research. Some common themes have emerged. One of the most significant is the involvement of KNOX genes in generating compound leaves, as in the tomato (see above). But, this is not universal. For example, the pea uses a different mechanism for doing the same thing.[79][80] Mutations in genes affecting leaf curvature can also change leaf form, by changing the leaf from flat, to a crinkly shape,[81] like the shape of cabbage leaves. There also exist different morphogen gradients in a developing leaf which define the leaf's axis and may also affect the leaf form. Another class of regulators of leaf development are the microRNAs.[82][83]

The roots (bottom image) of Lepidodendrales (Stigmaria) are thought to be developmentally equivalent to the stems (top), as the similar appearance of "leaf scars" and "root scars" on these specimens from different species demonstrates.

Roots are important to plants for two main reasons: Firstly, they provide anchorage to the substrate; more importantly, they provide a source of water and nutrients from the soil. Roots allowed plants to grow taller and faster.

The evolution of roots had consequences on a global scale. By disturbing the soil and promoting its acidification (by taking up nutrients such as nitrate and phosphate[84]), they enabled it to weather more deeply, injecting carbon compounds deeper into soils[85] with huge implications for climate.[86] These effects may have been so profound they led to a mass extinction.[87]

While there are traces of root-like impressions in fossil soils in the Late Silurian,[88] body fossils show the earliest plants to be devoid of roots. Many had prostrate branches that sprawled along the ground, with upright axes or thalli dotted here and there, and some even had non-photosynthetic subterranean branches which lacked stomata. The distinction between root and specialised branch is developmental. differing in their branching pattern and in possession of a root cap.[11] So while Siluro-Devonian plants such as Rhynia and Horneophyton possessed the physiological equivalent of roots,[89][90] roots – defined as organs differentiated from stems – did not arrive until later.[11] Unfortunately, roots are rarely preserved in the fossil record, and our understanding of their evolutionary origin is sparse.[11]

Rhizoids – small structures performing the same role as roots, usually a cell in diameter – probably evolved very early, perhaps even before plants colonised the land; they are recognised in the Characeae, an algal sister group to land plants.[11] That said, rhizoids probably evolved more than once; the rhizines of lichens, for example, perform a similar role. Even some animals (Lamellibrachia) have root-like structures.[11] Rhizoids are clearly visible in the Rhynie chert fossils, and were present in most of the earliest vascular plants, and on this basis seem to have presaged true plant roots.[91]

More advanced structures are common in the Rhynie chert, and many other fossils of comparable early Devonian age bear structures that look like, and acted like, roots.[11] The rhyniophytes bore fine rhizoids, and the trimerophytes and herbaceous lycopods of the chert bore root-like structure penetrating a few centimetres into the soil.[92] However, none of these fossils display all the features borne by modern roots,[11] with the exception of Asteroxylon, which has recently been recognized as bearing roots that evolved independently from those of extant vascular plants.[93] Roots and root-like structures became increasingly common and deeper penetrating during the Devonian, with lycopod trees forming roots around 20 cm long during the Eifelian and Givetian. These were joined by progymnosperms, which rooted up to about a metre deep, during the ensuing Frasnian stage.[92] True gymnosperms and zygopterid ferns also formed shallow rooting systems during the Famennian.[92]

The rhizophores of the lycopods provide a slightly different approach to rooting. They were equivalent to stems, with organs equivalent to leaves performing the role of rootlets.[11] A similar construction is observed in the extant lycopod Isoetes, and this appears to be evidence that roots evolved independently at least twice, in the lycophytes and other plants,[11] a proposition supported by studies showing that roots are initiated and their growth promoted by different mechanisms in lycophytes and euphyllophytes.[94]

A vascular system is indispensable to rooted plants, as non-photosynthesising roots need a supply of sugars, and a vascular system is required to transport water and nutrients from the roots to the rest of the plant.[10] Rooted plants are little more advanced than their Silurian forebears, without a dedicated root system; however, the flat-lying axes can be clearly seen to have growths similar to the rhizoids of bryophytes today.[95]

By the Middle to Late Devonian, most groups of plants had independently developed a rooting system of some nature.[95] As roots became larger, they could support larger trees, and the soil was weathered to a greater depth.[87] This deeper weathering had effects not only on the aforementioned drawdown of CO
2, but also opened up new habitats for colonisation by fungi and animals.[92]

Roots today have developed to the physical limits. They penetrate as much as 60 metres of soil to tap the water table.[96] The narrowest roots are a mere 40 μm in diameter, and could not physically transport water if they were any narrower.[11] The earliest fossil roots recovered, by contrast, narrowed from 3 mm to under 700 μm in diameter; of course, taphonomy is the ultimate control of what thickness can be seen.[11]

The trunk of early tree fern Psaronius, showing internal structure. The top of the plant would have been to the left of the image

External mold of Lepidodendron trunk showing leaf scars from the Upper Carboniferous of Ohio

The early Devonian landscape was devoid of vegetation taller than waist height. Greater height provided a competitive advantage in the harvesting of sunlight for photosynthesis, overshadowing of competitors and in spore distribution, as spores (and later, seeds) could be blown for greater distances if they started higher. An effective vascular system was required in order to achieve greater heights. To attain arborescence, plants had to develop woody tissue that provided both support and water transport, and thus needed to evolve the capacity for secondary growth. The stele of plants undergoing secondary growth is surrounded by a vascular cambium, a ring of meristematic cells which produces more xylem on the inside and phloem on the outside. Since xylem cells comprise dead, lignified tissue, subsequent rings of xylem are added to those already present, forming wood. Fossils of plants from the early Devonian show that a simple form of wood first appeared at least 400 million years ago, at a time when all land plants were small and herbaceous.[97] Because wood evolved long before shrubs and trees, it is likely that its original purpose was for water transport, and that it was only used for mechanical support later.[98]

The first plants to develop secondary growth and a woody habit, were apparently the ferns, and as early as the Middle Devonian one species, Wattieza, had already reached heights of 8 m and a tree-like habit.[99]

Other clades did not take long to develop a tree-like stature. The Late Devonian Archaeopteris, a precursor to gymnosperms which evolved from the trimerophytes,[100] reached 30 m in height. The progymnosperms were the first plants to develop true wood, grown from a bifacial cambium. The first appearance of one of them, Rellimia, was in the Middle Devonian.[101] True wood is only thought to have evolved once, giving rise to the concept of a "lignophyte" clade.

Archaeopteris forests were soon supplemented by arborescent lycopods, in the form of Lepidodendrales, which exceeded 50m in height and 2m across at the base. These arborescent lycopods rose to dominate Late Devonian and Carboniferous forests that gave rise to coal deposits.[102] Lepidodendrales differ from modern trees in exhibiting determinate growth: after building up a reserve of nutrients at a lower height, the plants would "bolt" as a single trunk to a genetically determined height, branch at that level, spread their spores and die.[103] They consisted of "cheap" wood to allow their rapid growth, with at least half of their stems comprising a pith-filled cavity.[6] Their wood was also generated by a unifacial vascular cambium – it did not produce new phloem, meaning that the trunks could not grow wider over time.

The horsetail Calamites appeared in the Carboniferous. Unlike the modern horsetail Equisetum, Calamites had a unifacial vascular cambium, allowing them to develop wood and grow to heights in excess of 10 m and to branch repeatedly.

While the form of early trees was similar to that of today's, the Spermatophytes or seed plants, the group that contain all modern trees, had yet to evolve. The dominant tree groups today are all seed plants, the gymnosperms, which include the coniferous trees, and the angiosperms, which contain all fruiting and flowering trees. No free-sporing trees like Archaeopteris exist in the extant flora. It was long thought that the angiosperms arose from within the gymnosperms, but recent molecular evidence suggests that their living representatives form two distinct groups.[104][105][106] The molecular data has yet to be fully reconciled with morphological data,[107][108][109] but it is becoming accepted that the morphological support for paraphyly is not especially strong.[110] This would lead to the conclusion that both groups arose from within the pteridosperms, probably as early as the Permian.[110]

The angiosperms and their ancestors played a very small role until they diversified during the Cretaceous. They started out as small, damp-loving organisms in the understorey, and have been diversifying ever since the mid-Cretaceous, to become the dominant member of non-boreal forests today.

The fossil seed Trigonocarpus

The transitional fossil Runcaria

Early land plants reproduced in the fashion of ferns: spores germinated into small gametophytes, which produced eggs and/or sperm. These sperm would swim across moist soils to find the female organs (archegonia) on the same or another gametophyte, where they would fuse with an egg to produce an embryo, which would germinate into a sporophyte.[92]

Heterosporic plants, as their name suggests, bear spores of two sizes – microspores and megaspores. These would germinate to form microgametophytes and megagametophytes, respectively. This system paved the way for ovules and seeds: taken to the extreme, the megasporangia could bear only a single megaspore tetrad, and to complete the transition to true ovules, three of the megaspores in the original tetrad could be aborted, leaving one megaspore per megasporangium.

The transition to ovules continued with this megaspore being "boxed in" to its sporangium while it germinated. Then, the megagametophyte was contained within a waterproof integument, which enclosed the seed. The pollen grain, which contained a microgametophyte germinated from a microspore , was employed for dispersal of the male gamete, only releasing its desiccation-prone flagellate sperm when it reached a receptive megagametophyte.[6]

Lycopods and sphenopsids got a fair way down the path to the seed habit without ever crossing the threshold. Fossil lycopod megaspores reaching 1 cm in diameter, and surrounded by vegetative tissue, are known (Lepidocarpon, Achlamydocarpon);– these even germinated into a megagametophyte in situ. However, they fell short of being ovules, since the nucellus, an inner spore-covering layer, does not completely enclose the spore. A very small slit (micropyle) remains, meaning that the megasporangium is still exposed to the atmosphere. This has two consequences – firstly, it means it is not fully resistant to desiccation, and secondly, sperm do not have to "burrow" to access the archegonia of the megaspore.[6]

A Middle Devonian precursor to seed plants from Belgium has been identified predating the earliest seed plants by about 20 million years. Runcaria, small and radially symmetrical, is an integumented megasporangium surrounded by a cupule. The megasporangium bears an unopened distal extension protruding above the multilobed integument. It is suspected that the extension was involved in anemophilous pollination. Runcaria sheds new light on the sequence of character acquisition leading to the seed. Runcaria has all of the qualities of seed plants except for a solid seed coat and a system to guide the pollen to the ovule.[111]

The first spermatophytes (literally: "seed plants") – that is, the first plants to bear true seeds – are called pteridosperms: literally, "seed ferns", so called because their foliage consisted of fern-like fronds, although they were not closely related to ferns. The oldest fossil evidence of seed plants is of Late Devonian age, and they appear to have evolved out of an earlier group known as the progymnosperms. These early seed plants ranged from trees to small, rambling shrubs; like most early progymnosperms, they were woody plants with fern-like foliage. They all bore ovules, but no cones, fruit or similar. While it is difficult to track the early evolution of seeds, the lineage of the seed ferns may be traced from the simple trimerophytes through homosporous Aneurophytes.[6]

This seed model is shared by basically all gymnosperms (literally: "naked seeds"), most of which encase their seeds in a woody cone or fleshy aril (the yew, for example), but none of which fully enclose their seeds. The angiosperms ("vessel seeds") are the only group to fully enclose the seed, in a carpel.

Fully enclosed seeds opened up a new pathway for plants to follow: that of seed dormancy. The embryo, completely isolated from the external atmosphere and hence protected from desiccation, could survive some years of drought before germinating. Gymnosperm seeds from the Late Carboniferous have been found to contain embryos, suggesting a lengthy gap between fertilisation and germination.[112] This period is associated with the entry into a greenhouse earth period, with an associated increase in aridity. This suggests that dormancy arose as a response to drier climatic conditions, where it became advantageous to wait for a moist period before germinating.[112] This evolutionary breakthrough appears to have opened a floodgate: previously inhospitable areas, such as dry mountain slopes, could now be tolerated, and were soon covered by trees.[112]

Seeds offered further advantages to their bearers: they increased the success rate of fertilised gametophytes, and because a nutrient store could be "packaged" in with the embryo, the seeds could germinate rapidly in inhospitable environments, reaching a size where it could fend for itself more quickly.[92] For example, without an endosperm, seedlings growing in arid environments would not have the reserves to grow roots deep enough to reach the water table before they expired from dehydration.[92] Likewise, seeds germinating in a gloomy understory require an additional reserve of energy to quickly grow high enough to capture sufficient light for self-sustenance.[92] A combination of these advantages gave seed plants the ecological edge over the previously dominant genus Archaeopteris, thus increasing the biodiversity of early forests.[92]

Despite these advantages, it is common for fertilized ovules to fail to mature as seeds.[113] Also during seed dormancy (often associated with unpredictable and stressful conditions) DNA damage accumulates.[114][115][116] Thus DNA damage appears to be a basic problem for survival of seed plants, just as DNA damage is a A major problem for life in general.[117]

The pollen bearing organs of the early "flower" Crossotheca

Flowers are modified leaves possessed only by the angiosperms, which are relatively late to appear in the fossil record. The group originated and diversified during the Early Cretaceous and became ecologically significant thereafter.[118] Flower-like structures first appear in the fossil records some ~130 mya, in the Cretaceous.[119] However, in 2018, scientists reported the finding of a fossil flower from about 180 million years ago, 50 million years earlier than thought earlier.[120] The interpretation has been however highly disputed.[121]

Colorful and/or pungent structures surround the cones of plants such as cycads and Gnetales, making a strict definition of the term "flower" elusive.[109]

The main function of a flower is reproduction, which, before the evolution of the flower and angiosperms, was the job of microsporophylls and megasporophylls. A flower can be considered a powerful evolutionary innovation, because its presence allowed the plant world to access new means and mechanisms for reproduction.

The evolution of syncarps.
a: sporangia borne at tips of leaf
b: Leaf curls up to protect sporangia
c: leaf curls to form enclosed roll
d: grouping of three rolls into a syncarp

The flowering plants have long been assumed to have evolved from within the gymnosperms; according to the traditional morphological view, they are closely allied to the Gnetales. However, as noted above, recent molecular evidence is at odds with this hypothesis,[105][106] and further suggests that Gnetales are more closely related to some gymnosperm groups than angiosperms,[104] and that extant gymnosperms form a distinct clade to the angiosperms,[104][105][106] the two clades diverging some 300 million years ago.[122]

The relationship of stem groups to the angiosperms is important in determining the evolution of flowers. Stem groups provide an insight into the state of earlier "forks" on the path to the current state. Convergence increases the risk of misidentifying stem groups. Since the protection of the megagametophyte is evolutionarily desirable, probably many separate groups evolved protective encasements independently. In flowers, this protection takes the form of a carpel, evolved from a leaf and recruited into a protective role, shielding the ovules. These ovules are further protected by a double-walled integument.

Penetration of these protective layers needs something more than a free-floating microgametophyte. Angiosperms have pollen grains comprising just three cells. One cell is responsible for drilling down through the integuments, and creating a conduit for the two sperm cells to flow down. The megagametophyte has just seven cells; of these, one fuses with a sperm cell, forming the nucleus of the egg itself, and another joins with the other sperm, and dedicates itself to forming a nutrient-rich endosperm. The other cells take auxiliary roles. This process of "double fertilisation" is unique and common to all angiosperms.

The inflorescences of the Bennettitales are strikingly similar to flowers

In the fossil record, there are three intriguing groups which bore flower-like structures. The first is the Permian pteridosperm Glossopteris, which already bore recurved leaves resembling carpels. The Mesozoic Caytonia is more flower-like still, with enclosed ovules – but only a single integument. Further, details of their pollen and stamens set them apart from true flowering plants.

The Bennettitales bore remarkably flower-like organs, protected by whorls of bracts which may have played a similar role to the petals and sepals of true flowers; however, these flower-like structures evolved independently, as the Bennettitales are more closely related to cycads and ginkgos than to the angiosperms.[123]

However, no true flowers are found in any groups save those extant today. Most morphological and molecular analyses place Amborella, the nymphaeales and Austrobaileyaceae in a basal clade called "ANA". This clade appear to have diverged in the early Cretaceous, around 130 million years ago – around the same time as the earliest fossil angiosperm,[124][125] and just after the first angiosperm-like pollen, 136 million years ago.[110] The magnoliids diverged soon after, and a rapid radiation had produced eudicots and monocots by 125 million years ago.[110] By the end of the Cretaceous 66 million years ago, over 50% of today's angiosperm orders had evolved, and the clade accounted for 70% of global species.[126] It was around this time that flowering trees became dominant over conifers.[6]^:498

The features of the basal "ANA" groups suggest that angiosperms originated in dark, damp, frequently disturbed areas.[127] It appears that the angiosperms remained constrained to such habitats throughout the Cretaceous – occupying the niche of small herbs early in the successional series.[126] This may have restricted their initial significance, but given them the flexibility that accounted for the rapidity of their later diversifications in other habitats.[127]

Some propose that the Angiosperms arose from an unknown Seed Fern, Pteridophyte, and view Cycads as living Seed Ferns with both Seed-Bearing and sterile leaves (Cycas revoluta)[108]

In August 2017, scientists presented a detailed description and 3D image of a reconstruction of possibly the first flower that lived about 140 million years ago.[128][129]

A male flower of Amborella trichopoda. Amborellaceae is considered the sister family of all other flowering plants.

The family Amborellaceae is regarded as being the sister clade to all other living flowering plants. A draft genome of Amborella trichopoda was published in December, 2013.[130] By comparing its genome with those of all other living flowering plants, it will be possible to work out the most likely characteristics of the ancestor of A. trichopoda and all other flowering plants, i.e. the ancestral flowering plant.[131]

It seems that on the level of the organ, the leaf may be the ancestor of the flower, or at least some floral organs. When some crucial genes involved in flower development are mutated, clusters of leaf-like structures arise in place of flowers. Thus, sometime in history, the developmental program leading to formation of a leaf must have been altered to generate a flower. There probably also exists an overall robust framework within which the floral diversity has been generated. An example of that is a gene called LEAFY (LFY), which is involved in flower development in Arabidopsis thaliana. The homologs of this gene are found in angiosperms as diverse as tomato, snapdragon, pea, maize and even gymnosperms. Expression of Arabidopsis thaliana LFY in distant plants like poplar and citrus also results in flower-production in these plants. The LFY gene regulates the expression of some genes belonging to the MADS-box family. These genes, in turn, act as direct controllers of flower development.

The members of the MADS-box family of transcription factors play a very important and evolutionarily conserved role in flower development. According to the ABC Model of flower development, three zones — A, B and C — are generated within the developing flower primordium, by the action of some transcription factors, that are members of the MADS-box family. Among these, the functions of the B and C domain genes have been evolutionarily more conserved than the A domain gene. Many of these genes have arisen through gene duplications of ancestral members of this family. Quite a few of them show redundant functions.

The evolution of the MADS-box family has been extensively studied. These genes are present even in pteridophytes, but the spread and diversity is many times higher in angiosperms.[132] There appears to be quite a bit of pattern into how this family has evolved. Consider the evolution of the C-region gene AGAMOUS (AG). It is expressed in today's flowers in the stamens, and the carpel, which are reproductive organs. Its ancestor in gymnosperms also has the same expression pattern. Here, it is expressed in the strobili, an organ that produces pollen or ovules.[133] Similarly, the B-genes' (AP3 and PI) ancestors are expressed only in the male organs in gymnosperms. Their descendants in the modern angiosperms also are expressed only in the stamens, the male reproductive organ. Thus, the same, then-existing components were used by the plants in a novel manner to generate the first flower. This is a recurring pattern in evolution.

There is enormous variation in floral structure in plants, typically due to changes in the MADS-box genes and their expression pattern. For example, grasses possess unique floral structures. The carpels and stamens are surrounded by scale-like lodicules and two bracts, the lemma and the palea, but genetic evidence and morphology suggest that lodicules are homologous to eudicot petals.[134] The palea and lemma may be homologous to sepals in other groups, or may be unique grass structures.

Another example is that of Linaria vulgaris, which has two kinds of flower symmetries-radial and bilateral. These symmetries are due to epigenetic changes in just one gene called CYCLOIDEA.[119]

Large number of petals in roses is the result of human selection

Arabidopsis thaliana has a gene called AGAMOUS that plays an important role in defining how many petals and sepals and other organs are generated. Mutations in this gene give rise to the floral meristem obtaining an indeterminate fate, and proliferation of floral organs in double-flowered forms of roses, carnations and morning glory. These phenotypes have been selected by horticulturists for their increased number of petals.[135] Several studies on diverse plants like petunia, tomato, Impatiens, maize etc. have suggested that the enormous diversity of flowers is a result of small changes in genes controlling their development.[136]

The Floral Genome Project confirmed that the ABC Model of flower development is not conserved across all angiosperms. Sometimes expression domains change, as in the case of many monocots, and also in some basal angiosperms like Amborella. Different models of flower development like the Fading boundaries model, or the Overlapping-boundaries model which propose non-rigid domains of expression, may explain these architectures.[137] There is a possibility that from the basal to the modern angiosperms, the domains of floral architecture have become more and more fixed through evolution.

Another floral feature that has been a subject of natural selection is flowering time. Some plants flower early in their life cycle, others require a period of vernalization before flowering. This outcome is based on factors like temperature, light intensity, presence of pollinators and other environmental signals: genes like CONSTANS (CO), Flowering Locus C (FLC) and FRIGIDA regulate integration of environmental signals into the pathway for flower development. Variations in these loci have been associated with flowering time variations between plants. For example, Arabidopsis thaliana ecotypes that grow in the cold, temperate regions require prolonged vernalization before they flower, while the tropical varieties, and the most common lab strains, don't. This variation is due to mutations in the FLC and FRIGIDA genes, rendering them non-functional.[138]

Many of the genes involved in this process are conserved across all the plants studied. Sometimes though, despite genetic conservation, the mechanism of action turns out to be different. For example, rice is a short-day plant, while Arabidopsis thaliana is a long-day plant. Both plants have the proteins CO and FLOWERING LOCUS T (FT), but, in Arabidopsis thaliana, CO enhances FT production, while in rice, the CO homolog represses FT production, resulting in completely opposite downstream effects.[139]

The Anthophyte theory was based on the observation that a gymnospermic group Gnetales has a flower-like ovule. It has partially developed vessels as found in the angiosperms, and the megasporangium is covered by three envelopes, like the ovary structure of angiosperm flowers. However, many other lines of evidence show that Gnetales is not related to angiosperms.[123]

The Mostly Male theory has a more genetic basis. Proponents of this theory point out that the gymnosperms have two very similar copies of the gene LFY, while angiosperms just have one. Molecular clock analysis has shown that the other LFY paralog was lost in angiosperms around the same time as flower fossils become abundant, suggesting that this event might have led to floral evolution.[140] According to this theory, loss of one of the LFY paralog led to flowers that were more male, with the ovules being expressed ectopically. These ovules initially performed the function of attracting pollinators, but sometime later, may have been integrated into the core flower.

The stem-loop secondary structure of a pre-microRNA from Brassica oleracea

While environmental factors are significantly responsible for evolutionary change, they act merely as agents for natural selection. Change is inherently brought about via phenomena at the genetic level: mutations, chromosomal rearrangements, and epigenetic changes. While the general types of mutations hold true across the living world, in plants, some other mechanisms have been implicated as highly significant.

Genome doubling is a relatively common occurrence in plant evolution and results in polyploidy, which is consequently a common feature in plants. It is estimated that at least half (and probably all) plants have seen genome doubling in their history. Genome doubling entails gene duplication, thus generating functional redundancy in most genes. The duplicated genes may attain new function, either by changes in expression pattern or changes in activity. Polyploidy and gene duplication are believed to be among the most powerful forces in evolution of plant form; though it is not known why genome doubling is such a frequent process in plants. One probable reason is the production of large amounts of secondary metabolites in plant cells. Some of them might interfere in the normal process of chromosomal segregation, causing genome duplication.

Top: teosinte, bottom: maize, middle: maize-teosinte hybrid

In recent times, plants have been shown to possess significant microRNA families, which are conserved across many plant lineages. In comparison to animals, while the number of plant miRNA families are lesser than animals, the size of each family is much larger. The miRNA genes are also much more spread out in the genome than those in animals, where they are more clustered. It has been proposed that these miRNA families have expanded by duplications of chromosomal regions.[141] Many miRNA genes involved in regulation of plant development have been found to be quite conserved between plants studied.

Domestication of plants like maize, rice, barley, wheat etc. has also been a significant driving force in their evolution. Research concerning the origin of maize has found that it is a domesticated derivative of a wild plant from Mexico called teosinte. Teosinte belongs to the genus Zea, just as maize, but bears very small inflorescence, 5–10 hard cobs and a highly branched and spread out stem.

Cauliflower – Brassica oleracea var. botrytis

Crosses between a particular teosinte variety and maize yields fertile offspring that are intermediate in phenotype between maize and teosinte. QTL analysis has also revealed some loci that, when mutated in maize, yield a teosinte-like stem or teosinte-like cobs. Molecular clock analysis of these genes estimates their origins to some 9,000 years ago, well in accordance with other records of maize domestication. It is believed that a small group of farmers must have selected some maize-like natural mutant of teosinte some 9,000 years ago in Mexico, and subjected it to continuous selection to yield the familiar maize plant of today.[142]

The edible cauliflower is a domesticated version of the wild plant Brassica oleracea, which does not possess the dense undifferentiated inflorescence, called the curd, that cauliflower possesses.

Wikispecies has information related to Brassicaceae.

Cauliflower possesses a single mutation in a gene called CAL, controlling meristem differentiation into inflorescence. This causes the cells at the floral meristem to gain an undifferentiated identity and, instead of growing into a flower, they grow into a dense mass of inflorescence meristem cells in arrested development.[143] This mutation has been selected through domestication since at least the time of the Greek empire.

C₄ plants evolved carbon concentrating mechanisms that work by increasing the concentration of CO
2 around RuBisCO, and excluding oxygen, thereby increasing the efficiency of photosynthesis by decreasing photorespiration. The process of concentrating CO
2 around RuBisCO requires more energy than allowing gases to diffuse, but under certain conditions – i.e. warm temperatures (>25 °C), low CO
2 concentrations, or high oxygen concentrations – pays off in terms of the decreased loss of sugars through photorespiration.

One type of C₄ metabolism employs a so-called Kranz anatomy. This transports CO
2 through an outer mesophyll layer, via a range of organic molecules, to the central bundle sheath cells, where the CO
2 is released. In this way, CO
2 is concentrated near the site of RuBisCO operation. Because RuBisCO is operating in an environment with much more CO
2 than it otherwise would be, it performs more efficiently.

A second mechanism, CAM photosynthesis, temporally separates photosynthesis from the action of RuBisCO. RuBisCO only operates during the day, when stomata are sealed and CO
2 is provided by the breakdown of the chemical malate. More CO
2 is then harvested from the atmosphere when stomata open, during the cool, moist nights, reducing water loss.

These two pathways, with the same effect on RuBisCO, evolved a number of times independently – indeed, C₄ alone arose 62 times in 18 different plant families. A number of 'pre-adaptations' seem to have paved the way for C4, leading to its clustering in certain clades: it has most frequently been innovated in plants that already had features such as extensive vascular bundle sheath tissue.[145] Many potential evolutionary pathways resulting in the C₄ phenotype are possible and have been characterised using Bayesian inference,[144] confirming that non-photosynthetic adaptations often provide evolutionary stepping stones for the further evolution of C₄.

The C₄ construction is used by a subset of grasses, while CAM is employed by many succulents and cacti. The C₄ trait appears to have emerged during the Oligocene, around 25 to 32 million years ago;[146] however, they did not become ecologically significant until the Miocene, 6 to 7 million years ago.[147] Remarkably, some charcoalified fossils preserve tissue organised into the Kranz anatomy, with intact bundle sheath cells,[148] allowing the presence C₄ metabolism to be identified. Isotopic markers are used to deduce their distribution and significance. C₃ plants preferentially use the lighter of two isotopes of carbon in the atmosphere, ¹²C, which is more readily involved in the chemical pathways involved in its fixation. Because C₄ metabolism involves a further chemical step, this effect is accentuated. Plant material can be analysed to deduce the ratio of the heavier ¹³C to ¹²C. This ratio is denoted δ¹³C. C₃ plants are on average around 14‰ (parts per thousand) lighter than the atmospheric ratio, while C₄ plants are about 28‰ lighter. The δ¹³C of CAM plants depends on the percentage of carbon fixed at night relative to what is fixed in the day, being closer to C₃ plants if they fix most carbon in the day and closer to C₄ plants if they fix all their carbon at night.[149]

Original fossil material in sufficient quantity to analyse the grass itself is scarce, but horses provide a good proxy. They were globally widespread in the period of interest, and browsed almost exclusively on grasses. There's an old phrase in isotope paleontology, "you are what you eat (plus a little bit)" – this refers to the fact that organisms reflect the isotopic composition of whatever they eat, plus a small adjustment factor. There is a good record of horse teeth throughout the globe, and their δ¹³C record shows a sharp negative inflection around 6 to 7 million years ago, during the Messinian that is interpreted as resulting from the rise of C₄ plants on a global scale.[147]

While C₄ enhances the efficiency of RuBisCO, the concentration of carbon is highly energy intensive. This means that C₄ plants only have an advantage over C₃ organisms in certain conditions: namely, high temperatures and low rainfall. C₄ plants also need high levels of sunlight to thrive.[150] Models suggest that, without wildfires removing shade-casting trees and shrubs, there would be no space for C₄ plants.[151] But, wildfires have occurred for 400 million years – why did C₄ take so long to arise, and then appear independently so many times? The Carboniferous (~300 million years ago) had notoriously high oxygen levels – almost enough to allow spontaneous combustion[152] – and very low CO
2, but there is no C₄ isotopic signature to be found. And there doesn't seem to be a sudden trigger for the Miocene rise.

During the Miocene, the atmosphere and climate were relatively stable. If anything, CO
2 increased gradually from 14 to 9 million years ago before settling down to concentrations similar to the Holocene.[153] This suggests that it did not have a key role in invoking C₄ evolution.[146] Grasses themselves (the group which would give rise to the most occurrences of C₄) had probably been around for 60 million years or more, so had had plenty of time to evolve C₄,[154][155] which, in any case, is present in a diverse range of groups and thus evolved independently. There is a strong signal of climate change in South Asia;[146] increasing aridity – hence increasing fire frequency and intensity – may have led to an increase in the importance of grasslands.[156] However, this is difficult to reconcile with the North American record.[146] It is possible that the signal is entirely biological, forced by the fire- (and elephant?)-[157] driven acceleration of grass evolution – which, both by increasing weathering and incorporating more carbon into sediments, reduced atmospheric CO
2 levels.[157] Finally, there is evidence that the onset of C₄ from 9 to 7 million years ago is a biased signal, which only holds true for North America, from where most samples originate; emerging evidence suggests that grasslands evolved to a dominant state at least 15Ma earlier in South America.