MRPS5

28S ribosomal protein S5, mitochondrial, otherwise called uS5m, is a protein that in humans is encoded by the MRPS5 gene.[4]

MRPS5
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesMRPS5, MRP-S5, S5mt, mitochondrial ribosomal protein S5, 28S ribosomal protein S5, mitochondrial
External IDsOMIM: 611972 MGI: 1924971 HomoloGene: 32726 GeneCards: MRPS5
Gene location (Human)
Chr.Chromosome 2 (human)[1]
Band2q11.1Start95,085,369 bp[1]
End95,149,434 bp[1]
Orthologs
SpeciesHumanMouse
Entrez

64969

77721

Ensembl

ENSG00000144029

ENSMUSG00000027374

UniProt

P82675

Q99N87

RefSeq (mRNA)

NM_031902
NM_001321995
NM_001321996
NM_001321997

NM_029963

RefSeq (protein)

NP_001308924
NP_001308925
NP_001308926
NP_114108

NP_084239

Location (UCSC)Chr 2: 95.09 – 95.15 Mbn/a
PubMed search[2][3]
Wikidata
View/Edit HumanView/Edit Mouse

Function

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q.[4]

Model organisms

Model organisms have been used in the study of MRPS5 function. A conditional knockout mouse line called Mrps5tm1b(EUCOMM)Wtsi was generated at the Wellcome Trust Sanger Institute.[5] Male and female animals underwent a standardized phenotypic screen[6] to determine the effects of deletion.[7][8][9][10] Additional screens performed: - In-depth immunological phenotyping[11]

Mrps5 knockout mouse phenotype
CharacteristicPhenotype
All data available at.[6][11]
Peripheral blood leukocytes 6 WeeksAbnormal
Haematology 6 WeeksAbnormal
InsulinNormal
Homozygous viability at P14Abnormal
Body weightNormal
Neurological assessmentNormal
Grip strengthNormal
DysmorphologyNormal
Indirect calorimetryNormal
Glucose tolerance testNormal
Auditory brainstem responseNormal
DEXANormal
RadiographyNormal
Eye morphologyNormal
Clinical chemistryNormal
Haematology 16 WeeksNormal
Peripheral blood leukocytes 16 WeeksNormal
Heart weightNormal
Salmonella infectionNormal
Cytotoxic T Cell FunctionNormal
Spleen ImmunophenotypingNormal
Mesenteric Lymph Node ImmunophenotypingNormal
Bone Marrow ImmunophenotypingNormal
Influenza ChallengeNormal

References
  1. GRCh38: Ensembl release 89: ENSG00000144029 - Ensembl, May 2017
  2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Entrez Gene: MRPS5 mitochondrial ribosomal protein S5".
  5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
  6. "International Mouse Phenotyping Consortium".
  7. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  8. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  9. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
  10. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
  11. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading
  • Overview of all the structural information available in the PDB for UniProt: P82675 (28S ribosomal protein S5, mitochondrial) at the PDBe-KB.
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