SPI1

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene.[5]

SPI1
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesSPI1, OF, PU.1, SFPI1, SPI-1, SPI-A, Spi-1 proto-oncogene
External IDsOMIM: 165170 MGI: 98282 HomoloGene: 2346 GeneCards: SPI1
Gene location (Human)
Chr.Chromosome 11 (human)[1]
Band11p11.2Start47,354,860 bp[1]
End47,378,576 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

6688

20375

Ensembl

ENSG00000066336

ENSMUSG00000002111

UniProt

P17947

P17433

RefSeq (mRNA)

NM_001080547
NM_003120

NM_011355
NM_001378898
NM_001378899

RefSeq (protein)

NP_001074016
NP_003111

NP_035485
NP_001365827
NP_001365828

Location (UCSC)Chr 11: 47.35 – 47.38 MbChr 2: 91.08 – 91.12 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Function

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development.[6] The nuclear protein binds to a purine-rich sequence known as the PU-box found on enhancers of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.[7]

Structure

The ETS domain is the DNA-binding module of PU.1 and other ETS-family transcription factors.

Interactions

SPI1 has been shown to interact with:

References

  1. GRCh38: Ensembl release 89: ENSG00000066336 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000002111 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990). "The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors". Oncogene. 5 (5): 663–668. PMID 1693183.
  6. Oikawa, T; Yamada, T; Kihara-Negishi, F; Yamamoto, H; Kondoh, N; Hitomi, Y; Hashimoto, Y (July 1999). "The role of Ets family transcription factor PU.1 in hematopoietic cell differentiation, proliferation and apoptosis". Cell Death Differ. 6 (7): 599–608. doi:10.1038/sj.cdd.4400534. PMID 10453070.
  7. "Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1".
  8. Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (February 1998). "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS". J. Biol. Chem. 273 (9): 4838–4842. doi:10.1074/jbc.273.9.4838. PMID 9478924.
  9. Zhang P, Behre G, Pan J, Iwama A, Wara-Aswapati N, Radomska HS, Auron PE, Tenen DG, Sun Z (July 1999). "Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1". Proc. Natl. Acad. Sci. U.S.A. 96 (15): 8705–8710. Bibcode:1999PNAS...96.8705Z. doi:10.1073/pnas.96.15.8705. PMC 17580. PMID 10411939.
  10. Brass AL, Zhu AQ, Singh H (February 1999). "Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers". EMBO J. 18 (4): 977–991. doi:10.1093/emboj/18.4.977. PMC 1171190. PMID 10022840.
  11. Escalante CR, Shen L, Escalante MC, Brass AL, Edwards TA, Singh H, Aggarwal AK (July 2002). "Crystallization and characterization of PU.1/IRF-4/DNA ternary complex". J. Struct. Biol. 139 (1): 55–59. doi:10.1016/s1047-8477(02)00514-2. PMID 12372320.
  12. Hallier M, Tavitian A, Moreau-Gachelin F (May 1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. 271 (19): 11177–11181. doi:10.1074/jbc.271.19.11177. PMID 8626664.

Further reading

This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.